Restriction map for
pFN21AE4467
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsaI
BseAI BseRI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI DrdI Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SfiI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 219 GTAC GT^AC
AflIII 1208 ACACGT A^C[GA][CT]GT
AhdI 1354 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 862 GTGCAC G^TGCAC
AlwI 984 GATCC GGATC(4/5)
AocI 1416 CCTAAGG CC^T[ACGT]AGG
ApaI 160 GGGCCC GGGCC^C
ApaLI 862 GTGCAC G^TGCAC
AspEI 1354 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI 1030 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 300 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 300 GCTCAGC GC^T[ACGT]AGC
BsaAI 859 TACGTG [CT]AC^GT[GA]
BsaWI 65 TCCGGT [AT]^CCGG[AT]
BsrI 1128 CCAGT ACTGG(1/-1)
BstAPI 925 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 938 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 983 AGATCC [GA]^GATC[CT]
Bsu36I 1416 CCTAAGG CC^T[ACGT]AGG
CelII 300 GCTCAGC GC^T[ACGT]AGC
CpoI 1280 CGGTCCG CG^G[AT]CCG
DraIII 340 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1354 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1416 CCTAAGG CC^T[ACGT]AGG
HincII 1204 GTCAAC GT[CT]^[GA]AC
HindII 1204 GTCAAC GT[CT]^[GA]AC
MflI 983 AGATCC [GA]^GATC[CT]
NotI 789 GCGGCCGC GC^GGCCGC
PstI 292 CTGCAG CTGCA^G
RsaI 219 GTAC GT^AC
RsrII 1280 CGGTCCG CG^G[AT]CCG
SapI 369 GCTCTTC GCTCTTC(1/4)
SexAI 845 ACCTGGT A^CC[AT]GGT
TfiI 690 GAATC G^A[AT]TC
XhoII 983 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 853 GTCTAC GT^[AC][GT]AC
910 GTCTAC
AvaI 58 CCCGAG C^[CT]CG[GA]G
1074 CCCGAG
BbeI 77 GGCGCC GGCGC^C
949 GGCGCC
BpmI 9 CTCCAG CTGGAG(16/14)
679 CTGGAG
BsoBI 58 CCCGAG C^[CT]CG[GA]G
1074 CCCGAG
DpnII 201 GATC ^GATC
984 GATC
DraII 159 GGGGCCC [GA]G^G[ACGT]CC[CT]
1265 GGGGCCT
EagI 790 CGGCCG C^GGCCG
833 CGGCCG
EclXI 790 CGGCCG C^GGCCG
833 CGGCCG
Eco52I 790 CGGCCG C^GGCCG
833 CGGCCG
EcoO109I 159 GGGGCCC [GA]G^G[ACGT]CC[CT]
1265 GGGGCCT
EcoT14I 386 CCATGG C^C[AT][AT]GG
1431 CCATGG
HgaI 380 GCGTC GACGC(5/10)
1022 GCGTC
KasI 77 GGCGCC G^GCGCC
949 GGCGCC
KspI 968 CCGCGG CCGC^GG
1116 CCGCGG
MboI 201 GATC ^GATC
984 GATC
MslI 204 CACGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
276 CACGCCGGTG
NaeI 457 GCCGGC GCC^GGC
835 GCCGGC
NarI 77 GGCGCC GG^CGCC
949 GGCGCC
NcoI 386 CCATGG C^CATGG
1431 CCATGG
NdeII 201 GATC ^GATC
984 GATC
NgoMIV 457 GCCGGC G^CCGGC
835 GCCGGC
NspI 353 ACATGC [GA]CATG^[CT]
1305 GCATGT
PleI 742 GACTC GAGTC(4/5)
536 GAGTC
PvuII 514 CAGCTG CAG^CTG
796 CAGCTG
SacII 968 CCGCGG CCGC^GG
1116 CCGCGG
Sau3AI 201 GATC ^GATC
984 GATC
SfcI 292 CTGCAG C^T[GA][CT]AG
375 CTACAG
SfoI 77 GGCGCC GGC^GCC
949 GGCGCC
SgrAI 206 CGCCGGTG C[GA]^CCGG[CT]G
278 CGCCGGTG
StyI 386 CCATGG C^C[AT][AT]GG
1431 CCATGG
XcmI 11 CCAGCCCAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
497 CCAGCTCCGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpuAI BsaHI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BssHII BssKI BssSI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PflMI SacI Sau96I ScrFI
SfaNI TseI Tsp45I TspRI Van91I