(Circular) (Six-base) MAPSORT of: pFN21AB5784.seq Check: 9392 from: 1 to: 7015 pFN21AB5784 7015 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4003 4003 Size: 7015 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4825 278 Size: 53 83 186 936 3289 2468 Fragments arranged by size: 3289 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2663 2987 4358 1937 Size: 726 324 1371 4594 Fragments arranged by size: 4594 1371 726 324 Acc65I G'GTAC_C Cuts at: 4336 4336 Size: 7015 AclI AA'CG_TT Cuts at: 1563 5142 5515 1563 Size: 3579 373 3063 Fragments arranged by size: 3579 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3333 3387 3600 5078 6462 6730 1632 Size: 1701 54 213 1478 1384 268 1917 Fragments arranged by size: 1917 1701 1478 1384 268 213 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6818 AflIII A'CryG_T Cuts at: 1203 2412 2658 5914 6891 1203 Size: 1209 246 3256 977 1327 Fragments arranged by size: 3256 1327 1209 977 246 AgeI A'CCGG_T Cuts at: 5846 5846 Size: 7015 AhdI GACnn_n'nnGTC Cuts at: 5744 5744 Size: 7015 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7015 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4011 1184 Size: 2827 4188 AlwNI CAG_nnn'CTG Cuts at: 3713 3809 4758 6330 3713 Size: 96 949 1572 4398 Fragments arranged by size: 4398 1572 949 96 ApaI G_GGCC'C Cuts at: 3009 3495 3728 3009 Size: 486 233 6296 Fragments arranged by size: 6296 486 233 ApaLI G'TGCA_C Cuts at: 5072 6228 5072 Size: 1156 5859 ApoI r'AATT_y Cuts at: 1493 2746 2971 4324 4550 4702 6811 1493 Size: 1253 225 1353 226 152 2109 1697 Fragments arranged by size: 2109 1697 1353 1253 226 225 152 AseI AT'TA_AT Cuts at: 160 1032 5569 160 Size: 872 4537 1606 Fragments arranged by size: 4537 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 AvaI C'yCGr_G Cuts at: 1870 1943 4340 1870 Size: 73 2397 4545 Fragments arranged by size: 4545 2397 73 BamHI G'GATC_C Cuts at: 4291 4345 4712 4291 Size: 54 367 6594 Fragments arranged by size: 6594 367 54 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3742 4336 5791 618 Size: 325 217 627 1955 594 1455 1842 Fragments arranged by size: 1955 1842 1455 627 594 325 217 BanII G_rGCy'C Cuts at: 1391 3009 3495 3728 4334 1391 Size: 1618 486 233 606 4072 Fragments arranged by size: 4072 1618 606 486 233 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3339 3556 928 Size: 971 1440 217 4387 Fragments arranged by size: 4387 1440 971 217 BbvCI CC'TCA_GC Cuts at: 3864 3864 Size: 7015 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5206 5240 1903 Size: 34 3269 34 3678 Fragments arranged by size: 3678 3269 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4907 6117 4907 Size: 1210 5805 BclI T'GATC_A Cuts at: 1548 1548 Size: 7015 BfrBI ATG'CAT Cuts at: 4202 4202 Size: 7015 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4253 5626 136 Size: 107 122 71 631 605 2581 1373 1525 Fragments arranged by size: 2581 1525 1373 631 605 122 107 71 BglII A'GATC_T Cuts at: 3051 7010 3051 Size: 3959 3056 BlpI GC'TnA_GC Cuts at: 4432 4432 Size: 7015 Bme1580I G_kGCm'C Cuts at: 1790 3009 3495 3728 4005 5076 6232 1790 Size: 1219 486 233 277 1071 1156 2573 Fragments arranged by size: 2573 1219 1156 1071 486 277 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7015 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2340 3479 4746 Size: 416 53 83 399 24 923 1139 1267 Cuts at: 4746 5704 442 Size: 958 1753 Fragments arranged by size: 1753 1267 1139 958 923 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2937 3690 3886 5675 1239 Size: 1698 753 196 1789 2579 Fragments arranged by size: 2579 1789 1698 753 196 Bpu10I CC'TnA_GC Cuts at: 3081 3864 3081 Size: 783 6232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5140 6005 6303 5140 Size: 865 298 5852 Fragments arranged by size: 5852 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2262 5678 882 Size: 617 763 3416 2219 Fragments arranged by size: 3416 2219 763 617 BsaAI yAC'GTr Cuts at: 493 1204 2413 4806 493 Size: 711 1209 2393 2702 Fragments arranged by size: 2702 2393 1209 711 BsaBI GATnn'nnATC Cuts at: 2115 4116 7009 2115 Size: 2001 2893 2121 Fragments arranged by size: 2893 2121 2001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3413 4822 5204 5839 Size: 53 83 186 936 1880 1409 382 635 Cuts at: 5839 275 Size: 1451 Fragments arranged by size: 1880 1451 1409 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3060 5448 5846 6120 6267 6678 3060 Size: 2388 398 274 147 411 3397 Fragments arranged by size: 3397 2388 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3460 3490 3688 3718 1178 Size: 30 165 30 2057 30 198 30 4475 Fragments arranged by size: 4475 2057 198 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2436 3375 3832 3849 3852 1186 Size: 1250 939 457 17 3 4349 Fragments arranged by size: 4349 1250 939 457 17 3 BseYI C'CCAG_C Cuts at: 3117 6218 6714 3117 Size: 3101 496 3418 Fragments arranged by size: 3418 3101 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6442 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4465 5226 6254 1540 Size: 275 186 2464 761 1028 2301 Fragments arranged by size: 2464 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3478 5076 5161 6232 3478 Size: 1598 85 1071 4261 Fragments arranged by size: 4261 1598 1071 85 BsmI GAATG_Cn' Cuts at: 4077 4096 4526 4619 4077 Size: 19 430 93 6473 Fragments arranged by size: 6473 430 93 19 BsmBI CGTCTCn'nnnn_ Cuts at: 3397 3397 Size: 7015 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3009 3478 3495 3728 4005 4334 5076 Size: 399 1219 469 17 233 277 329 742 Cuts at: 5076 5161 6232 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1219 1071 742 469 399 329 277 233 85 17 BspEI T'CCGG_A Cuts at: 6678 6678 Size: 7015 BspHI T'CATG_A Cuts at: 4904 6634 4904 Size: 1730 5285 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4003 4370 844 Size: 3159 367 3489 Fragments arranged by size: 3489 3159 367 BsrBI CCG'CTC Cuts at: 1394 1823 4902 1394 Size: 429 3079 3507 Fragments arranged by size: 3507 3079 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2373 5510 5684 65 Size: 1183 1125 3137 174 1396 Fragments arranged by size: 3137 1396 1183 1125 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5659 5846 1700 Size: 110 3849 187 2869 Fragments arranged by size: 3849 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7015 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7015 BssSI C'ACGA_G Cuts at: 5075 6087 5075 Size: 1012 6003 BstAPI GCAn_nnn'nTGC Cuts at: 794 3749 794 Size: 2955 4060 BstBI TT'CG_AA Cuts at: 5819 5819 Size: 7015 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7015 BstXI CCAn_nnnn'nTGG Cuts at: 3454 3454 Size: 7015 BstYI r'GATC_y Cuts at: 1972 2280 2716 3051 3457 4291 4345 4712 5097 Size: 308 436 335 406 834 54 367 385 Cuts at: 5097 5114 6566 6652 6664 7010 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 834 436 406 385 367 346 335 308 86 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2814 3531 4083 2814 Size: 717 552 5746 Fragments arranged by size: 5746 717 552 BtgI C'CryG_G Cuts at: 513 1065 3092 3542 513 Size: 552 2027 450 3986 Fragments arranged by size: 3986 2027 552 450 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2154 487 Size: 683 56 928 5348 Fragments arranged by size: 5348 928 683 56 BtsI GCAGTG_nn' Cuts at: 3132 3915 4531 5325 5345 3132 Size: 783 616 794 20 4802 Fragments arranged by size: 4802 794 783 616 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7015 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6980 DraI TTT'AAA Cuts at: 4222 4319 4222 Size: 97 6918 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3343 6022 809 Size: 507 2027 2679 1802 Fragments arranged by size: 2679 2027 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4462 5351 6980 Size: 54 1425 50 275 18 2632 889 1629 Cuts at: 6980 8 Size: 43 Fragments arranged by size: 2632 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4462 1537 Size: 275 2650 4090 Fragments arranged by size: 4090 2650 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2802 3319 3467 4945 1353 Size: 1449 517 148 1478 3423 Fragments arranged by size: 3423 1478 1449 517 148 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5602 5976 6122 387 Size: 1064 138 4013 374 146 1280 Fragments arranged by size: 4013 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2937 3333 3387 3600 3690 3886 5078 Size: 393 1305 396 54 213 90 196 1192 Cuts at: 5078 5675 6462 6730 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1305 1192 787 597 396 393 268 213 196 90 54 EcoNI CCTnn'n_nnAGG Cuts at: 3213 3534 3213 Size: 321 6694 EcoO109I rG'GnC_Cy Cuts at: 2096 2587 3006 3491 3492 3724 3725 2096 Size: 491 419 485 1 232 1 5386 Fragments arranged by size: 5386 491 485 419 232 1 1 EcoRI G'AATT_C Cuts at: 2746 2971 4324 4702 2746 Size: 225 1353 378 5059 Fragments arranged by size: 5059 1353 378 225 EcoRV GAT'ATC Cuts at: 2704 2704 Size: 7015 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3265 3265 Size: 7015 FspI TGC'GCA Cuts at: 5521 5521 Size: 7015 HaeII r_GCGC'y Cuts at: 1678 6162 1678 Size: 4484 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2708 2740 3038 3070 3592 Size: 32 135 32 1136 32 298 32 522 Cuts at: 3592 3624 5656 5688 5730 5762 1373 Size: 32 2032 32 42 32 2626 Fragments arranged by size: 2626 2032 1136 522 298 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2290 2635 3025 3611 3759 4359 Size: 988 281 352 345 390 586 148 600 Cuts at: 4359 4605 669 Size: 246 3079 Fragments arranged by size: 3079 988 600 586 390 352 345 281 246 148 HindIII A'AGCT_T Cuts at: 748 748 Size: 7015 HpaI GTT'AAC Cuts at: 4605 4605 Size: 7015 KpnI G_GTAC'C Cuts at: 4340 4340 Size: 7015 MfeI C'AATT_G Cuts at: 4614 4614 Size: 7015 MluI A'CGCG_T Cuts at: 6891 6891 Size: 7015 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2711 3647 4003 6129 6313 1305 Size: 1406 936 356 2126 184 2007 Fragments arranged by size: 2126 2007 1406 936 356 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5193 Fragments arranged by size: 5193 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4973 5332 5491 518 Size: 648 3807 359 159 2042 Fragments arranged by size: 3807 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2249 2995 3656 4431 5108 6256 6501 1606 Size: 643 746 661 775 677 1148 245 2120 Fragments arranged by size: 2120 1148 775 746 677 661 643 245 NcoI C'CATG_G Cuts at: 513 1065 3092 3542 513 Size: 552 2027 450 3986 Fragments arranged by size: 3986 2027 552 450 NdeI CA'TA_TG Cuts at: 387 387 Size: 7015 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7015 NotI GC'GGCC_GC Cuts at: 4462 4462 Size: 7015 NsiI A_TGCA'T Cuts at: 4204 4204 Size: 7015 NspI r_CATG'y Cuts at: 1132 1229 2662 4373 5918 1132 Size: 97 1433 1711 1545 2229 Fragments arranged by size: 2229 1711 1545 1433 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7015 PciI A'CATG_T Cuts at: 2658 5914 2658 Size: 3256 3759 PflMI CCAn_nnn'nTGG Cuts at: 1108 3753 1108 Size: 2645 4370 PfoI T'CCnGG_A Cuts at: 3589 3589 Size: 7015 PmeI GTTT'AAAC Cuts at: 4319 4319 Size: 7015 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3155 3187 5084 5116 6597 6629 3155 Size: 32 1897 32 1481 32 3541 Fragments arranged by size: 3541 1897 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7015 PsiI TTA'TAA Cuts at: 4585 4585 Size: 7015 PspOMI G'GGCC_C Cuts at: 3005 3491 3724 3005 Size: 486 233 6296 Fragments arranged by size: 6296 486 233 PstI C_TGCA'G Cuts at: 830 3444 4367 830 Size: 2614 923 3478 Fragments arranged by size: 3478 2614 923 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7015 PvuII CAG'CTG Cuts at: 2249 3656 2249 Size: 1407 5608 SalI G'TCGA_C Cuts at: 1936 4357 1936 Size: 2421 4594 SbfI CC_TGCA'GG Cuts at: 4367 4367 Size: 7015 ScaI AGT'ACT Cuts at: 5263 6975 5263 Size: 1712 5303 SfcI C'TryA_G Cuts at: 826 1045 3432 3440 4363 5498 6179 6370 826 Size: 219 2387 8 923 1135 681 191 1471 Fragments arranged by size: 2387 1471 1135 923 681 219 191 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7015 SmaI CCC'GGG Cuts at: 1872 4342 1872 Size: 2470 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5119 6020 6282 820 Size: 197 926 3176 901 262 1553 Fragments arranged by size: 3176 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4806 493 Size: 4313 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7015 SphI G_CATG'C Cuts at: 1132 4373 1132 Size: 3241 3774 SspI AAT'ATT Cuts at: 5 52 3796 4939 5 Size: 47 3744 1143 2081 Fragments arranged by size: 3744 2081 1143 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7015 StyI C'CwwG_G Cuts at: 513 1065 3092 3542 3891 4454 513 Size: 552 2027 450 349 563 3074 Fragments arranged by size: 3074 2027 563 552 450 349 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2244 3433 3593 3760 5042 5059 5212 Size: 1255 664 1189 160 167 1282 17 153 Cuts at: 5212 5397 5842 325 Size: 185 445 1498 Fragments arranged by size: 1498 1282 1255 1189 664 445 185 167 160 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5261 6973 96 Size: 274 80 33 51 1443 3284 1712 138 Fragments arranged by size: 3284 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2860 2892 4027 4059 6975 7007 2860 Size: 32 1135 32 2916 32 2868 Fragments arranged by size: 2916 2868 1135 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7015 XbaI T'CTAG_A Cuts at: 2599 4351 2599 Size: 1752 5263 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7015 XmaI C'CCGG_G Cuts at: 1870 4340 1870 Size: 2470 4545 XmnI GAAnn'nnTTC Cuts at: 3167 4214 5144 3167 Size: 1047 930 5038 Fragments arranged by size: 5038 1047 930 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4823 276 Size: 53 83 186 936 3289 2468 Fragments arranged by size: 3289 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI