################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KspI MaeII MamI MfeI MluI MroI MspA1I MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 1442 GGTACC G^GTACC AccII 588 CGCG CG^CG AcsI 645 GAATTC [GA]^AATT[CT] AocI 37 CCTGAGG CC^T[ACGT]AGG ApaI 257 GGGCCC GGGCC^C ApoI 645 GAATTC [GA]^AATT[CT] Asp718I 1442 GGTACC G^GTACC AspHI 184 GTGCTC G[AT]GC[AT]^C AspI 1229 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1518 GGATCC G^GATCC BglII 107 AGATCT A^GATCT BlpI 1245 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1245 GCTCAGC GC^T[ACGT]AGC BsaI 208 GGTCTC GGTCTC(1/5) BseRI 1496 GAGGAG GAGGAG(10/8) BsgI 1278 CTGCAC GTGCAG(16/14) BsiEI 1402 CGACCG CG[GA][CT]^CG BsiHKAI 184 GTGCTC G[AT]GC[AT]^C BsrBI 1060 GAGCGG CCGCTC(-3/-3) BstUI 588 CGCG CG^CG Bsu36I 37 CCTGAGG CC^T[ACGT]AGG CelII 1245 GCTCAGC GC^T[ACGT]AGC CfoI 1209 GCGC GCG^C Eco81I 37 CCTGAGG CC^T[ACGT]AGG EcoRI 645 GAATTC G^AATTC EcoT14I 176 CCAAGG C^C[AT][AT]GG HapII 82 CCGG C^CGG HhaI 1209 GCGC GCG^C HinP1I 1209 GCGC G^CGC HincII 4 GTTGAC GT[CT]^[GA]AC HindII 4 GTTGAC GT[CT]^[GA]AC HpaII 82 CCGG C^CGG KpnI 1442 GGTACC GGTAC^C MspI 82 CCGG C^CGG MvnI 588 CGCG CG^CG PflFI 1229 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 356 GACTC GAGTC(4/5) PpuMI 1451 AGGTCCT [GA]G^G[AT]CC[CT] PstI 1472 CTGCAG CTGCA^G SapI 1086 GCTCTTC GCTCTTC(1/4) SspI 918 AATATT AAT^ATT StyI 176 CCAAGG C^C[AT][AT]GG TaqI 1227 TCGA T^CGA Tth111I 1229 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1227 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AvaI 156 CTCGGG C^[CT]CG[GA]G 444 CCCGAG AvaII 362 GGACC G^G[AT]CC 1452 GGTCC BalI 618 TGGCCA TGG^CCA 872 TGGCCA BanI 280 GGCACC G^G[CT][GA]CC 1442 GGTACC BbsI 1126 GAAGAC GAAGAC(2/6) 1267 GTCTTC BfaI 853 CTAG C^TAG 995 CTAG BpmI 1447 CTCCAG CTGGAG(16/14) 322 CTGGAG BpuAI 1126 GAAGAC GAAGAC(2/6) 1267 GTCTTC BsmFI 725 GGGAC GGGAC(10/14) 1235 GTCCC BsoBI 156 CTCGGG C^[CT]CG[GA]G 444 CCCGAG BspMI 773 ACCTGC ACCTGC(4/8) 87 GCAGGT BstXI 419 CCAACTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1283 CCATCTTCCTGG BstYI 107 AGATCT [GA]^GATC[CT] 1518 GGATCC EaeI 618 TGGCCA [CT]^GGCC[GA] 872 TGGCCA MaeI 853 CTAG C^TAG 995 CTAG MflI 107 AGATCT [GA]^GATC[CT] 1518 GGATCC MluNI 618 TGGCCA TGG^CCA 872 TGGCCA MscI 618 TGGCCA TGG^CCA 872 TGGCCA SfcI 1152 CTACAG C^T[GA][CT]AG 1472 CTGCAG SmlI 1014 CTCAAG C^T[CT][GA]AG 1240 CTCAAG StuI 326 AGGCCT AGG^CCT 1356 AGGCCT TfiI 338 GATTC G^A[AT]TC 759 GAATC Tsp509I 646 AATT ^AATT 933 AATT XcmI 470 CCAGCCGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1283 CCATCTTCCTGGTGG XhoII 107 AGATCT [GA]^GATC[CT] 1518 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BanII BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I TspRI