(Circular) (Six-base) MAPSORT of: pFN21AE6403.seq Check: 5794 from: 1 to: 6238 pFN21AE6403 6238 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2083 2083 Size: 6238 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3581 1937 Size: 1644 4594 Acc65I G'GTAC_C Cuts at: 3447 3559 3447 Size: 112 6126 AclI AA'CG_TT Cuts at: 1563 3537 4365 4738 1563 Size: 1974 828 373 3063 Fragments arranged by size: 3063 1974 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2772 3148 4301 5685 5953 1632 Size: 491 649 376 1153 1384 268 1917 Fragments arranged by size: 1917 1384 1153 649 491 376 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 5137 6114 1203 Size: 3934 977 1327 Fragments arranged by size: 3934 1327 977 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 3077 1166 Size: 1911 4327 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2819 1184 Size: 1635 4603 AlwNI CAG_nnn'CTG Cuts at: 2364 3171 3460 3476 3981 5553 2364 Size: 807 289 16 505 1572 3049 Fragments arranged by size: 3049 1572 807 505 289 16 ApaI G_GGCC'C Cuts at: 2266 2266 Size: 6238 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 2650 3547 3773 3925 6034 1493 Size: 1157 897 226 152 2109 1697 Fragments arranged by size: 2109 1697 1157 897 226 152 AseI AT'TA_AT Cuts at: 160 1032 4792 160 Size: 872 3760 1606 Fragments arranged by size: 3760 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 2161 2449 3563 1870 Size: 73 218 288 1114 4545 Fragments arranged by size: 4545 1114 288 218 73 BamHI G'GATC_C Cuts at: 3523 3568 3935 3523 Size: 45 367 5826 Fragments arranged by size: 5826 367 45 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2285 3447 3559 5014 618 Size: 325 217 627 498 1162 112 1455 1842 Fragments arranged by size: 1842 1455 1162 627 498 325 217 112 BanII G_rGCy'C Cuts at: 1391 2266 2391 3020 3557 1391 Size: 875 125 629 537 4072 Fragments arranged by size: 4072 875 629 537 125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3138 3265 928 Size: 971 1239 127 3901 Fragments arranged by size: 3901 1239 971 127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4429 4463 1903 Size: 34 2492 34 3678 Fragments arranged by size: 3678 2492 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 1548 Size: 6238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4849 136 Size: 107 122 71 631 605 3177 1525 Fragments arranged by size: 3177 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2112 6233 2112 Size: 4121 2117 BlpI GC'TnA_GC Cuts at: 3251 3655 3251 Size: 404 5834 Bme1580I G_kGCm'C Cuts at: 1790 2266 2288 4299 5455 1790 Size: 476 22 2011 1156 2573 Fragments arranged by size: 2573 2011 1156 476 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2291 3969 4927 Size: 416 53 83 399 24 874 1678 958 Cuts at: 4927 442 Size: 1753 Fragments arranged by size: 1753 1678 958 874 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 3437 4898 1239 Size: 1109 1089 1461 2579 Fragments arranged by size: 2579 1461 1109 1089 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3004 3230 4363 5228 5526 3004 Size: 226 1133 865 298 3716 Fragments arranged by size: 3716 1133 865 298 226 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2219 4901 882 Size: 617 720 2682 2219 Fragments arranged by size: 2682 2219 720 617 BsaAI yAC'GTr Cuts at: 493 1204 4029 493 Size: 711 2825 2702 Fragments arranged by size: 2825 2702 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4045 4427 5062 275 Size: 53 83 186 936 2512 382 635 1451 Fragments arranged by size: 2512 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4671 5069 5343 5490 5901 4671 Size: 398 274 147 411 5008 Fragments arranged by size: 5008 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 1178 Size: 30 165 30 786 30 5197 Fragments arranged by size: 5197 786 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3516 1186 Size: 2330 3908 BseYI C'CCAG_C Cuts at: 2037 2727 5441 5937 2037 Size: 690 2714 496 2338 Fragments arranged by size: 2714 2338 690 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3268 1163 Size: 573 1532 4133 Fragments arranged by size: 4133 1532 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3410 3688 4449 5477 1540 Size: 275 186 1409 278 761 1028 2301 Fragments arranged by size: 2301 1409 1028 761 278 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 4299 4384 5455 2193 Size: 2106 85 1071 2976 Fragments arranged by size: 2976 2106 1071 85 BsmI GAATG_Cn' Cuts at: 3749 3842 3749 Size: 93 6145 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2266 2288 2391 3020 3557 4299 Size: 399 403 73 22 103 629 537 742 Cuts at: 4299 4384 5455 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 629 537 403 399 103 85 73 22 BspEI T'CCGG_A Cuts at: 5901 5901 Size: 6238 BspHI T'CATG_A Cuts at: 4127 5857 4127 Size: 1730 4508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2083 2787 3593 844 Size: 1239 704 806 3489 Fragments arranged by size: 3489 1239 806 704 BsrBI CCG'CTC Cuts at: 1394 1823 3067 4125 1394 Size: 429 1244 1058 3507 Fragments arranged by size: 3507 1244 1058 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2406 2685 3342 3348 4733 4907 65 Size: 1183 1158 279 657 6 1385 174 1396 Fragments arranged by size: 1396 1385 1183 1158 657 279 174 6 BsrFI r'CCGG_y Cuts at: 1700 1810 4882 5069 1700 Size: 110 3072 187 2869 Fragments arranged by size: 3072 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 4298 5310 4298 Size: 1012 5226 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6238 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6238 BstXI CCAn_nnnn'nTGG Cuts at: 2431 3295 2431 Size: 864 5374 BstYI r'GATC_y Cuts at: 1972 2112 3523 3568 3935 4320 4337 5789 5875 Size: 140 1411 45 367 385 17 1452 86 Cuts at: 5875 5887 6233 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1411 385 367 346 140 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 2043 2043 Size: 6238 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4747 Fragments arranged by size: 4747 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5499 Fragments arranged by size: 5499 683 56 BtsI GCAGTG_nn' Cuts at: 2117 2682 3754 4548 4568 2117 Size: 565 1072 794 20 3787 Fragments arranged by size: 3787 1072 794 565 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3542 3542 Size: 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2623 2877 3685 Size: 54 1425 50 275 18 793 254 808 Cuts at: 3685 4574 6203 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 808 793 275 254 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2252 2450 2748 3098 4168 1353 Size: 899 198 298 350 1070 3423 Fragments arranged by size: 3423 1070 899 350 298 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4825 5199 5345 387 Size: 1064 138 3236 374 146 1280 Fragments arranged by size: 3236 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2348 2772 3148 3437 4301 4898 Size: 393 491 225 424 376 289 864 597 Cuts at: 4898 5685 5953 1239 Size: 787 268 1524 Fragments arranged by size: 1524 864 787 597 491 424 393 376 289 268 225 EcoO109I rG'GnC_Cy Cuts at: 2242 2262 2263 3457 2242 Size: 20 1 1194 5023 Fragments arranged by size: 5023 1194 20 1 EcoRI G'AATT_C Cuts at: 2650 3547 3925 2650 Size: 897 378 4963 Fragments arranged by size: 4963 897 378 FspI TGC'GCA Cuts at: 4744 4744 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 5385 1678 Size: 3707 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2651 2683 2749 2781 3515 Size: 32 135 32 1079 32 66 32 734 Cuts at: 3515 3547 4879 4911 4953 4985 1373 Size: 32 1332 32 42 32 2626 Fragments arranged by size: 2626 1332 1079 734 135 66 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2011 3582 3828 669 Size: 988 281 73 1571 246 3079 Fragments arranged by size: 3079 1571 988 281 246 73 HindIII A'AGCT_T Cuts at: 748 748 Size: 6238 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KpnI G_GTAC'C Cuts at: 3451 3563 3451 Size: 112 6126 MfeI C'AATT_G Cuts at: 3837 3837 Size: 6238 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5352 5536 1305 Size: 4047 184 2007 Fragments arranged by size: 4047 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2625 2879 10 Size: 54 1425 343 793 254 3369 Fragments arranged by size: 3369 1425 793 343 254 54 MslI CAynn'nnrTG Cuts at: 518 1166 2801 3050 3077 3389 4196 4555 4714 Size: 648 1635 249 27 312 807 359 159 Cuts at: 4714 518 Size: 2042 Fragments arranged by size: 2042 1635 807 648 359 312 249 159 27 MspA1I CmG'CkG Cuts at: 1606 3654 4331 5479 5724 1606 Size: 2048 677 1148 245 2120 Fragments arranged by size: 2120 2048 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4747 Fragments arranged by size: 4747 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 3596 5141 1132 Size: 97 2367 1545 2229 Fragments arranged by size: 2367 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2081 1348 Size: 733 5505 PciI A'CATG_T Cuts at: 5137 5137 Size: 6238 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6238 PfoI T'CCnGG_A Cuts at: 2325 2325 Size: 6238 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3457 3457 Size: 6238 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6238 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PspOMI G'GGCC_C Cuts at: 2262 2262 Size: 6238 PstI C_TGCA'G Cuts at: 830 3481 3590 830 Size: 2651 109 3478 Fragments arranged by size: 3478 2651 109 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SapI GCTCTTCn'nnn_ Cuts at: 3098 3098 Size: 6238 SbfI CC_TGCA'GG Cuts at: 3590 3590 Size: 6238 ScaI AGT'ACT Cuts at: 4486 6198 4486 Size: 1712 4526 SfcI C'TryA_G Cuts at: 826 1045 3157 3477 3586 4721 5402 5593 826 Size: 219 2112 320 109 1135 681 191 1471 Fragments arranged by size: 2112 1471 1135 681 320 219 191 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3019 3245 4342 5243 5505 820 Size: 197 926 1076 226 1097 901 262 1553 Fragments arranged by size: 1553 1097 1076 926 901 262 226 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 3596 1132 Size: 2464 3774 SspI AAT'ATT Cuts at: 5 52 2925 4162 5 Size: 47 2873 1237 2081 Fragments arranged by size: 2873 2081 1237 47 StuI AGG'CCT Cuts at: 1517 2333 3363 1517 Size: 816 1030 4392 Fragments arranged by size: 4392 1030 816 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 3677 513 Size: 552 939 177 1496 3074 Fragments arranged by size: 3074 1496 939 552 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2054 2669 4265 4282 4435 4620 5065 Size: 1255 474 615 1596 17 153 185 445 Cuts at: 5065 325 Size: 1498 Fragments arranged by size: 1596 1498 1255 615 474 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2700 4484 6196 Size: 274 80 33 51 1443 723 1784 1712 Cuts at: 6196 96 Size: 138 Fragments arranged by size: 1784 1712 1443 723 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6198 6230 6198 Size: 32 6206 Tth111I GACn'n_nGTC Cuts at: 1316 3237 1316 Size: 1921 4317 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2482 3295 2482 Size: 813 5425 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6238 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 4367 4367 Size: 6238 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI