(Linear) (Six-base) MAPSORT of: KIEE6403.seq Check: 5827 from: 1 to: 1533 KIEE6403.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 78 1533 Size: 78 1455 Acc65I G'GTAC_C Cuts at: 0 1442 1533 Size: 1442 91 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 767 1143 1533 Size: 118 649 376 390 Fragments arranged by size: 649 390 376 118 AleI CACnn'nnGTG Cuts at: 0 1072 1533 Size: 1072 461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 814 1533 Size: 814 719 AlwNI CAG_nnn'CTG Cuts at: 0 359 1166 1455 1471 1533 Size: 359 807 289 16 62 Fragments arranged by size: 807 359 289 62 16 ApaI G_GGCC'C Cuts at: 0 261 1533 Size: 261 1272 ApoI r'AATT_y Cuts at: 0 645 1533 Size: 645 888 AvaI C'yCGr_G Cuts at: 0 156 444 1533 Size: 156 288 1089 Fragments arranged by size: 1089 288 156 BamHI G'GATC_C Cuts at: 0 1518 1533 Size: 1518 15 BanI G'GyrC_C Cuts at: 0 280 1442 1533 Size: 280 1162 91 Fragments arranged by size: 1162 280 91 BanII G_rGCy'C Cuts at: 0 261 386 1015 1533 Size: 261 125 629 518 Fragments arranged by size: 629 518 261 125 BbsI GAAGACnn'nnnn_ Cuts at: 0 1133 1260 1533 Size: 1133 127 273 Fragments arranged by size: 1133 273 127 BglII A'GATC_T Cuts at: 0 107 1533 Size: 107 1426 BlpI GC'TnA_GC Cuts at: 0 1246 1533 Size: 1246 287 Bme1580I G_kGCm'C Cuts at: 0 261 283 1533 Size: 261 22 1250 Fragments arranged by size: 1250 261 22 BmrI ACTGGGnnnn_n' Cuts at: 0 286 1533 Size: 286 1247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 1432 1533 Size: 343 1089 101 Fragments arranged by size: 1089 343 101 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 999 1225 1533 Size: 999 226 308 Fragments arranged by size: 999 308 226 BsaI GGTCTCn'nnnn_ Cuts at: 0 214 1533 Size: 214 1319 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 184 214 1533 Size: 184 30 1319 Fragments arranged by size: 1319 184 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1511 1533 Size: 1511 22 BseYI C'CCAG_C Cuts at: 0 32 722 1533 Size: 32 690 811 Fragments arranged by size: 811 690 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1263 1533 Size: 1263 270 BsiEI CG_ry'CG Cuts at: 0 1405 1533 Size: 1405 128 BsiHKAI G_wGCw'C Cuts at: 0 188 1533 Size: 188 1345 Bsp1286I G_dGCh'C Cuts at: 0 188 261 283 386 1015 1533 Size: 188 73 22 103 629 518 Fragments arranged by size: 629 518 188 103 73 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 78 782 1533 Size: 78 704 751 Fragments arranged by size: 751 704 78 BsrBI CCG'CTC Cuts at: 0 1062 1533 Size: 1062 471 BsrDI GCAATG_nn' Cuts at: 0 401 680 1337 1343 1533 Size: 401 279 657 6 190 Fragments arranged by size: 657 401 279 190 6 BstXI CCAn_nnnn'nTGG Cuts at: 0 426 1290 1533 Size: 426 864 243 Fragments arranged by size: 864 426 243 BstYI r'GATC_y Cuts at: 0 107 1518 1533 Size: 107 1411 15 Fragments arranged by size: 1411 107 15 Bsu36I CC'TnA_GG Cuts at: 0 38 1533 Size: 38 1495 BtsI GCAGTG_nn' Cuts at: 0 112 677 1533 Size: 112 565 856 Fragments arranged by size: 856 565 112 EaeI y'GGCC_r Cuts at: 0 618 872 1533 Size: 618 254 661 Fragments arranged by size: 661 618 254 EarI CTCTTCn'nnn_ Cuts at: 0 247 445 743 1093 1533 Size: 247 198 298 350 440 Fragments arranged by size: 440 350 298 247 198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 343 767 1143 1432 1533 Size: 118 225 424 376 289 101 Fragments arranged by size: 424 376 289 225 118 101 EcoO109I rG'GnC_Cy Cuts at: 0 237 257 258 1452 1533 Size: 237 20 1 1194 81 Fragments arranged by size: 1194 237 81 20 1 EcoRI G'AATT_C Cuts at: 0 645 1533 Size: 645 888 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 646 678 744 776 1510 1533 Size: 646 32 66 32 734 23 Fragments arranged by size: 734 646 66 32 32 23 HincII GTy'rAC Cuts at: 0 6 1533 Size: 6 1527 KpnI G_GTAC'C Cuts at: 0 1446 1533 Size: 1446 87 MscI TGG'CCA Cuts at: 0 620 874 1533 Size: 620 254 659 Fragments arranged by size: 659 620 254 MslI CAynn'nnrTG Cuts at: 0 796 1045 1072 1384 1533 Size: 796 249 27 312 149 Fragments arranged by size: 796 312 249 149 27 PasI CC'CwG_GG Cuts at: 0 76 1533 Size: 76 1457 PfoI T'CCnGG_A Cuts at: 0 320 1533 Size: 320 1213 PpuMI rG'GwC_Cy Cuts at: 0 1452 1533 Size: 1452 81 PspOMI G'GGCC_C Cuts at: 0 257 1533 Size: 257 1276 PstI C_TGCA'G Cuts at: 0 1476 1533 Size: 1476 57 SapI GCTCTTCn'nnn_ Cuts at: 0 1093 1533 Size: 1093 440 SfcI C'TryA_G Cuts at: 0 1152 1472 1533 Size: 1152 320 61 Fragments arranged by size: 1152 320 61 SmlI C'TyrA_G Cuts at: 0 1014 1240 1533 Size: 1014 226 293 Fragments arranged by size: 1014 293 226 SspI AAT'ATT Cuts at: 0 920 1533 Size: 920 613 StuI AGG'CCT Cuts at: 0 328 1358 1533 Size: 328 1030 175 Fragments arranged by size: 1030 328 175 StyI C'CwwG_G Cuts at: 0 176 1533 Size: 176 1357 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 49 664 1533 Size: 49 615 869 Fragments arranged by size: 869 615 49 TatI w'GTAC_w Cuts at: 0 695 1533 Size: 695 838 Tth111I GACn'n_nGTC Cuts at: 0 1232 1533 Size: 1232 301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 477 1290 1533 Size: 477 813 243 Fragments arranged by size: 813 477 243 Enzymes that do cut: AarI Acc65I AcuI AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstXI BstYI Bsu36I BtsI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I HincII KpnI MscI MslI PasI PfoI PpuMI PspOMI PstI SapI SfcI SmlI SspI StuI StyI TaqII TatI Tth111I XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI XbaI XhoI XmaI XmnI ZraI