Restriction map for pFN21AE1415
 (Circular) (Six-base) MAPSORT of: pFN21AE1415.seq  Check: 5884  from: 1  to: 5476



pFN21AE1415  5476 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2293    2324    2350    2465    2293 
   Size:         31      26     115    5304

  Fragments arranged by size: 

                5304     115      31      26
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3286     278 
   Size:         53      83     186     936    1750    2468

  Fragments arranged by size: 

                2468    1750     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2176    2819    1937 
   Size:        239     643    4594

  Fragments arranged by size: 

                4594     643     239
 
Acc65I G'GTAC_C

Cuts at:   2797    2797 
   Size:       5476
 
AclI AA'CG_TT

Cuts at:   1563    3603    3976    1563 
   Size:       2040     373    3063

  Fragments arranged by size: 

                3063    2040     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2478    3539    4923    5191    1632 
   Size:        846    1061    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1061     846     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5279
 
AflIII A'CryG_T

Cuts at:   1203    4375    5352    1203 
   Size:       3172     977    1327

  Fragments arranged by size: 

                3172    1327     977
 
AgeI A'CCGG_T

Cuts at:   4307    4307 
   Size:       5476
 
AhdI GACnn_n'nnGTC

Cuts at:   4205    4205 
   Size:       5476
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5476
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2305    1184 
   Size:       1121    4355
 
AlwNI CAG_nnn'CTG

Cuts at:   2032    3219    4791    2032 
   Size:       1187    1572    2717

  Fragments arranged by size: 

                2717    1572    1187
 
ApaI G_GGCC'C

Cuts at:   2349    2718    2349 
   Size:        369    5107
 
ApaLI G'TGCA_C

Cuts at:   3533    4689    3533 
   Size:       1156    4320
 
ApoI r'AATT_y

Cuts at:   1493    2785    3011    3163    5272    1493 
   Size:       1292     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1292     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4030     160 
   Size:        872    2998    1606

  Fragments arranged by size: 

                2998    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5476
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2150    2801    1870 
   Size:         73     207     651    4545

  Fragments arranged by size: 

                4545     651     207      73
 
BamHI G'GATC_C

Cuts at:   2352    2806    3173    2352 
   Size:        454     367    4655

  Fragments arranged by size: 

                4655     454     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2281    2797    4252     618 
   Size:        325     217     627     494     516    1455    1842

  Fragments arranged by size: 

                1842    1455     627     516     494     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2349    2718    2795    1391 
   Size:        958     369      77    4072

  Fragments arranged by size: 

                4072     958     369      77
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4505
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3667    3701    1903 
   Size:         34    1730      34    3678

  Fragments arranged by size: 

                3678    1730      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3368    4578    3368 
   Size:       1210    4266
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5476
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4087     136 
   Size:        107     122      71     631     605    2415    1525

  Fragments arranged by size: 

                2415    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5471    5471 
   Size:       5476
 
BlpI GC'TnA_GC

Cuts at:   2010    2741    2771    2893    2010 
   Size:        731      30     122    4593

  Fragments arranged by size: 

                4593     731     122      30
 
Bme1580I G_kGCm'C

Cuts at:   1790    2349    2403    2718    3537    4693    1790 
   Size:        559      54     315     819    1156    2573

  Fragments arranged by size: 

                2573    1156     819     559     315      54
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5476
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3207    4165     442 
   Size:        416      53      83     399      24    1790     958    1753

  Fragments arranged by size: 

                1790    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5476
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2203    4136    1239 
   Size:        964    1933    2579

  Fragments arranged by size: 

                2579    1933     964
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3601    4466    4764    3601 
   Size:        865     298    4313

  Fragments arranged by size: 

                4313     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4139     882 
   Size:        617    2640    2219

  Fragments arranged by size: 

                2640    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3267     493 
   Size:        711    2063    2702

  Fragments arranged by size: 

                2702    2063     711
 
BsaBI GATnn'nnATC

Cuts at:   5470    5470 
   Size:       5476
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3283    3665    4300     275 
   Size:         53      83     186     936    1750     382     635    1451

  Fragments arranged by size: 

                1750    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3909    4307    4581    4728    5139    3909 
   Size:        398     274     147     411    4246

  Fragments arranged by size: 

                4246     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2315    2345    1178 
   Size:         30     165      30     912      30    4309

  Fragments arranged by size: 

                4309     912     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2687    1186 
   Size:       1501    3975
 
BseYI C'CCAG_C

Cuts at:   2650    2711    4679    5175    2650 
   Size:         61    1968     496    2951

  Fragments arranged by size: 

                2951    1968     496      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4903
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2926    3687    4715    1540 
   Size:        275     186     925     761    1028    2301

  Fragments arranged by size: 

                2301    1028     925     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2333    3537    3622    4693    2333 
   Size:       1204      85    1071    3116

  Fragments arranged by size: 

                3116    1204    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2197    2987    3080    2197 
   Size:        790      93    4593

  Fragments arranged by size: 

                4593     790      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2333    2349    2403    2718    2795    3537    3622 
   Size:        399     543      16      54     315      77     742      85

Cuts at:   3622    4693    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     543     399     315      85      77
                  54      16
 
BspEI T'CCGG_A

Cuts at:   5139    5139 
   Size:       5476
 
BspHI T'CATG_A

Cuts at:   3365    5095    3365 
   Size:       1730    3746
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2293    2324    2350    2465    2831     844 
   Size:       1449      31      26     115     366    3489

  Fragments arranged by size: 

                3489    1449     366     115      31      26
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2386    3363    1394 
   Size:        429     563     977    3507

  Fragments arranged by size: 

                3507     977     563     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2432    2578    3971    4145      65 
   Size:       1183    1184     146    1393     174    1396

  Fragments arranged by size: 

                1396    1393    1184    1183     174     146
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4120    4307    1700 
   Size:        110    2310     187    2869

  Fragments arranged by size: 

                2869    2310     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5476
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5476
 
BssSI C'ACGA_G

Cuts at:   3536    4548    3536 
   Size:       1012    4464
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5476
 
BstBI TT'CG_AA

Cuts at:   4280    4280 
   Size:       5476
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5476
 
BstYI r'GATC_y

Cuts at:   1972    2352    2806    3173    3558    3575    5027    5113    5125 
   Size:        380     454     367     385      17    1452      86      12

Cuts at:   5125    5471    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     454     385     380     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3985

  Fragments arranged by size: 

                3985     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4737

  Fragments arranged by size: 

                4737     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2177    2520    2992    3786    3806    2177 
   Size:        343     472     794      20    3847

  Fragments arranged by size: 

                3847     794     472     343      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5476
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5441
 
DraI TTT'AAA

Cuts at:   2780    2780 
   Size:       5476
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4483     809 
   Size:        507    3167    1802

  Fragments arranged by size: 

                3167    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2623    2923    3812 
   Size:         54    1425      50     275      18     793     300     889

Cuts at:   3812    5441       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     793     300     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2923    1537 
   Size:        275    1111    4090

  Fragments arranged by size: 

                4090    1111     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3406    1353 
   Size:       2053    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4063    4437    4583     387 
   Size:       1064     138    2474     374     146    1280

  Fragments arranged by size: 

                2474    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2203    2478    3539    4136    4923    5191    1239 
   Size:        393     571     275    1061     597     787     268    1524

  Fragments arranged by size: 

                1524    1061     787     597     571     393     275     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2147    2345    2346    2715    2147 
   Size:        198       1     369    4908

  Fragments arranged by size: 

                4908     369     198       1
 
EcoRI G'AATT_C

Cuts at:   2785    3163    2785 
   Size:        378    5098
 
FspI TGC'GCA

Cuts at:   2512    3982    2512 
   Size:       1470    4006
 
HaeII r_GCGC'y

Cuts at:   1678    4623    1678 
   Size:       2945    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4117    4149    4191    4223    1373 
   Size:         32     135      32    2545      32      42      32    2626

  Fragments arranged by size: 

                2626    2545     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2177    2820    3066     669 
   Size:        988     281     239     643     246    3079

  Fragments arranged by size: 

                3079     988     643     281     246     239
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5476
 
HpaI GTT'AAC

Cuts at:   3066    3066 
   Size:       5476
 
KpnI G_GTAC'C

Cuts at:   2801    2801 
   Size:       5476
 
MfeI C'AATT_G

Cuts at:   3075    3075 
   Size:       5476
 
MluI A'CGCG_T

Cuts at:   5352    5352 
   Size:       5476
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4590    4774    1305 
   Size:       3285     184    2007

  Fragments arranged by size: 

                3285    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3654

  Fragments arranged by size: 

                3654    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2232    2636    3434    3793    3952     518 
   Size:        648    1066     404     798     359     159    2042

  Fragments arranged by size: 

                2042    1066     798     648     404     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2014    2029    2628    2892    3569    4717    4962    1606 
   Size:        408      15     599     264     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     599     408     264     245      15
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3985

  Fragments arranged by size: 

                3985     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5476
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5476
 
NotI GC'GGCC_GC

Cuts at:   2923    2923 
   Size:       5476
 
NspI r_CATG'y

Cuts at:   1132    1229    2834    4379    1132 
   Size:         97    1605    1545    2229

  Fragments arranged by size: 

                2229    1605    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2656    2718    1348 
   Size:       1308      62    4106

  Fragments arranged by size: 

                4106    1308      62
 
PciI A'CATG_T

Cuts at:   4375    4375 
   Size:       5476
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2362    1108 
   Size:       1254    4222
 
PfoI T'CCnGG_A

Cuts at:   2307    2307 
   Size:       5476
 
PmeI GTTT'AAAC

Cuts at:   2780    2780 
   Size:       5476
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3545    3577    5058    5090    3545 
   Size:         32    1481      32    3931

  Fragments arranged by size: 

                3931    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5476
 
PsiI TTA'TAA

Cuts at:   3046    3046 
   Size:       5476
 
PspOMI G'GGCC_C

Cuts at:   2345    2714    2345 
   Size:        369    5107
 
PstI C_TGCA'G

Cuts at:    830    2698    2828     830 
   Size:       1868     130    3478

  Fragments arranged by size: 

                3478    1868     130
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5476
 
PvuII CAG'CTG

Cuts at:   2029    2029 
   Size:       5476
 
SalI G'TCGA_C

Cuts at:   1936    2175    2818    1936 
   Size:        239     643    4594

  Fragments arranged by size: 

                4594     643     239
 
SbfI CC_TGCA'GG

Cuts at:   2828    2828 
   Size:       5476
 
ScaI AGT'ACT

Cuts at:   3724    5436    3724 
   Size:       1712    3764
 
SfcI C'TryA_G

Cuts at:    826    1045    2055    2608    2694    2824    3959    4640    4831 
   Size:        219    1010     553      86     130    1135     681     191

Cuts at:   4831     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135    1010     681     553     219     191     130
                  86
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5476
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5476
 
SmaI CCC'GGG

Cuts at:   1872    2803    1872 
   Size:        931    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3580    4481    4743     820 
   Size:        197     926    1637     901     262    1553

  Fragments arranged by size: 

                1637    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3267     493 
   Size:       2774    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5476
 
SphI G_CATG'C

Cuts at:   1132    2834    1132 
   Size:       1702    3774
 
SspI AAT'ATT

Cuts at:      5      52    3400       5 
   Size:         47    3348    2081

  Fragments arranged by size: 

                3348    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5476
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2915     513 
   Size:        552     939     911    3074

  Fragments arranged by size: 

                3074     939     911     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2038    2572    3503    3520    3673    3858    4303 
   Size:       1255     458     534     931      17     153     185     445

Cuts at:   4303     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255     931     534     458     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3722    5434      96 
   Size:        274      80      33      51    1443    1745    1712     138

  Fragments arranged by size: 

                1745    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2254    2286    5436    5468    2254 
   Size:         32    3150      32    2262

  Fragments arranged by size: 

                3150    2262      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5476
 
XbaI T'CTAG_A

Cuts at:   2812    2812 
   Size:       5476
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2674    2674 
   Size:       5476
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5476
 
XmaI C'CCGG_G

Cuts at:   1870    2801    1870 
   Size:        931    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3605    3605 
   Size:       5476
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3284     276 
   Size:         53      83     186     936    1750    2468

  Fragments arranged by size: 

                2468    1750     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com