(Circular) (Six-base) MAPSORT of: pFN21AE1415.seq Check: 5884 from: 1 to: 5476 pFN21AE1415 5476 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2293 2324 2350 2465 2293 Size: 31 26 115 5304 Fragments arranged by size: 5304 115 31 26 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3286 278 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2176 2819 1937 Size: 239 643 4594 Fragments arranged by size: 4594 643 239 Acc65I G'GTAC_C Cuts at: 2797 2797 Size: 5476 AclI AA'CG_TT Cuts at: 1563 3603 3976 1563 Size: 2040 373 3063 Fragments arranged by size: 3063 2040 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2478 3539 4923 5191 1632 Size: 846 1061 1384 268 1917 Fragments arranged by size: 1917 1384 1061 846 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5279 AflIII A'CryG_T Cuts at: 1203 4375 5352 1203 Size: 3172 977 1327 Fragments arranged by size: 3172 1327 977 AgeI A'CCGG_T Cuts at: 4307 4307 Size: 5476 AhdI GACnn_n'nnGTC Cuts at: 4205 4205 Size: 5476 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2305 1184 Size: 1121 4355 AlwNI CAG_nnn'CTG Cuts at: 2032 3219 4791 2032 Size: 1187 1572 2717 Fragments arranged by size: 2717 1572 1187 ApaI G_GGCC'C Cuts at: 2349 2718 2349 Size: 369 5107 ApaLI G'TGCA_C Cuts at: 3533 4689 3533 Size: 1156 4320 ApoI r'AATT_y Cuts at: 1493 2785 3011 3163 5272 1493 Size: 1292 226 152 2109 1697 Fragments arranged by size: 2109 1697 1292 226 152 AseI AT'TA_AT Cuts at: 160 1032 4030 160 Size: 872 2998 1606 Fragments arranged by size: 2998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 AvaI C'yCGr_G Cuts at: 1870 1943 2150 2801 1870 Size: 73 207 651 4545 Fragments arranged by size: 4545 651 207 73 BamHI G'GATC_C Cuts at: 2352 2806 3173 2352 Size: 454 367 4655 Fragments arranged by size: 4655 454 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2281 2797 4252 618 Size: 325 217 627 494 516 1455 1842 Fragments arranged by size: 1842 1455 627 516 494 325 217 BanII G_rGCy'C Cuts at: 1391 2349 2718 2795 1391 Size: 958 369 77 4072 Fragments arranged by size: 4072 958 369 77 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4505 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3667 3701 1903 Size: 34 1730 34 3678 Fragments arranged by size: 3678 1730 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3368 4578 3368 Size: 1210 4266 BclI T'GATC_A Cuts at: 1548 1548 Size: 5476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4087 136 Size: 107 122 71 631 605 2415 1525 Fragments arranged by size: 2415 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5471 5471 Size: 5476 BlpI GC'TnA_GC Cuts at: 2010 2741 2771 2893 2010 Size: 731 30 122 4593 Fragments arranged by size: 4593 731 122 30 Bme1580I G_kGCm'C Cuts at: 1790 2349 2403 2718 3537 4693 1790 Size: 559 54 315 819 1156 2573 Fragments arranged by size: 2573 1156 819 559 315 54 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5476 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3207 4165 442 Size: 416 53 83 399 24 1790 958 1753 Fragments arranged by size: 1790 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2203 4136 1239 Size: 964 1933 2579 Fragments arranged by size: 2579 1933 964 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3601 4466 4764 3601 Size: 865 298 4313 Fragments arranged by size: 4313 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4139 882 Size: 617 2640 2219 Fragments arranged by size: 2640 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3267 493 Size: 711 2063 2702 Fragments arranged by size: 2702 2063 711 BsaBI GATnn'nnATC Cuts at: 5470 5470 Size: 5476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3283 3665 4300 275 Size: 53 83 186 936 1750 382 635 1451 Fragments arranged by size: 1750 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3909 4307 4581 4728 5139 3909 Size: 398 274 147 411 4246 Fragments arranged by size: 4246 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 1178 Size: 30 165 30 912 30 4309 Fragments arranged by size: 4309 912 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2687 1186 Size: 1501 3975 BseYI C'CCAG_C Cuts at: 2650 2711 4679 5175 2650 Size: 61 1968 496 2951 Fragments arranged by size: 2951 1968 496 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4903 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2926 3687 4715 1540 Size: 275 186 925 761 1028 2301 Fragments arranged by size: 2301 1028 925 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2333 3537 3622 4693 2333 Size: 1204 85 1071 3116 Fragments arranged by size: 3116 1204 1071 85 BsmI GAATG_Cn' Cuts at: 2197 2987 3080 2197 Size: 790 93 4593 Fragments arranged by size: 4593 790 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2333 2349 2403 2718 2795 3537 3622 Size: 399 543 16 54 315 77 742 85 Cuts at: 3622 4693 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 543 399 315 85 77 54 16 BspEI T'CCGG_A Cuts at: 5139 5139 Size: 5476 BspHI T'CATG_A Cuts at: 3365 5095 3365 Size: 1730 3746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2293 2324 2350 2465 2831 844 Size: 1449 31 26 115 366 3489 Fragments arranged by size: 3489 1449 366 115 31 26 BsrBI CCG'CTC Cuts at: 1394 1823 2386 3363 1394 Size: 429 563 977 3507 Fragments arranged by size: 3507 977 563 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2432 2578 3971 4145 65 Size: 1183 1184 146 1393 174 1396 Fragments arranged by size: 1396 1393 1184 1183 174 146 BsrFI r'CCGG_y Cuts at: 1700 1810 4120 4307 1700 Size: 110 2310 187 2869 Fragments arranged by size: 2869 2310 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5476 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5476 BssSI C'ACGA_G Cuts at: 3536 4548 3536 Size: 1012 4464 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5476 BstBI TT'CG_AA Cuts at: 4280 4280 Size: 5476 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5476 BstYI r'GATC_y Cuts at: 1972 2352 2806 3173 3558 3575 5027 5113 5125 Size: 380 454 367 385 17 1452 86 12 Cuts at: 5125 5471 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 454 385 380 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3985 Fragments arranged by size: 3985 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4737 Fragments arranged by size: 4737 683 56 BtsI GCAGTG_nn' Cuts at: 2177 2520 2992 3786 3806 2177 Size: 343 472 794 20 3847 Fragments arranged by size: 3847 794 472 343 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5441 DraI TTT'AAA Cuts at: 2780 2780 Size: 5476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4483 809 Size: 507 3167 1802 Fragments arranged by size: 3167 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2623 2923 3812 Size: 54 1425 50 275 18 793 300 889 Cuts at: 3812 5441 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 793 300 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2923 1537 Size: 275 1111 4090 Fragments arranged by size: 4090 1111 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3406 1353 Size: 2053 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4063 4437 4583 387 Size: 1064 138 2474 374 146 1280 Fragments arranged by size: 2474 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2203 2478 3539 4136 4923 5191 1239 Size: 393 571 275 1061 597 787 268 1524 Fragments arranged by size: 1524 1061 787 597 571 393 275 268 EcoO109I rG'GnC_Cy Cuts at: 2147 2345 2346 2715 2147 Size: 198 1 369 4908 Fragments arranged by size: 4908 369 198 1 EcoRI G'AATT_C Cuts at: 2785 3163 2785 Size: 378 5098 FspI TGC'GCA Cuts at: 2512 3982 2512 Size: 1470 4006 HaeII r_GCGC'y Cuts at: 1678 4623 1678 Size: 2945 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4117 4149 4191 4223 1373 Size: 32 135 32 2545 32 42 32 2626 Fragments arranged by size: 2626 2545 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2177 2820 3066 669 Size: 988 281 239 643 246 3079 Fragments arranged by size: 3079 988 643 281 246 239 HindIII A'AGCT_T Cuts at: 748 748 Size: 5476 HpaI GTT'AAC Cuts at: 3066 3066 Size: 5476 KpnI G_GTAC'C Cuts at: 2801 2801 Size: 5476 MfeI C'AATT_G Cuts at: 3075 3075 Size: 5476 MluI A'CGCG_T Cuts at: 5352 5352 Size: 5476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4590 4774 1305 Size: 3285 184 2007 Fragments arranged by size: 3285 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3654 Fragments arranged by size: 3654 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2232 2636 3434 3793 3952 518 Size: 648 1066 404 798 359 159 2042 Fragments arranged by size: 2042 1066 798 648 404 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2029 2628 2892 3569 4717 4962 1606 Size: 408 15 599 264 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 599 408 264 245 15 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3985 Fragments arranged by size: 3985 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5476 NotI GC'GGCC_GC Cuts at: 2923 2923 Size: 5476 NspI r_CATG'y Cuts at: 1132 1229 2834 4379 1132 Size: 97 1605 1545 2229 Fragments arranged by size: 2229 1605 1545 97 PasI CC'CwG_GG Cuts at: 1348 2656 2718 1348 Size: 1308 62 4106 Fragments arranged by size: 4106 1308 62 PciI A'CATG_T Cuts at: 4375 4375 Size: 5476 PflMI CCAn_nnn'nTGG Cuts at: 1108 2362 1108 Size: 1254 4222 PfoI T'CCnGG_A Cuts at: 2307 2307 Size: 5476 PmeI GTTT'AAAC Cuts at: 2780 2780 Size: 5476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 5058 5090 3545 Size: 32 1481 32 3931 Fragments arranged by size: 3931 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5476 PsiI TTA'TAA Cuts at: 3046 3046 Size: 5476 PspOMI G'GGCC_C Cuts at: 2345 2714 2345 Size: 369 5107 PstI C_TGCA'G Cuts at: 830 2698 2828 830 Size: 1868 130 3478 Fragments arranged by size: 3478 1868 130 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5476 PvuII CAG'CTG Cuts at: 2029 2029 Size: 5476 SalI G'TCGA_C Cuts at: 1936 2175 2818 1936 Size: 239 643 4594 Fragments arranged by size: 4594 643 239 SbfI CC_TGCA'GG Cuts at: 2828 2828 Size: 5476 ScaI AGT'ACT Cuts at: 3724 5436 3724 Size: 1712 3764 SfcI C'TryA_G Cuts at: 826 1045 2055 2608 2694 2824 3959 4640 4831 Size: 219 1010 553 86 130 1135 681 191 Cuts at: 4831 826 Size: 1471 Fragments arranged by size: 1471 1135 1010 681 553 219 191 130 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5476 SmaI CCC'GGG Cuts at: 1872 2803 1872 Size: 931 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3580 4481 4743 820 Size: 197 926 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3267 493 Size: 2774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5476 SphI G_CATG'C Cuts at: 1132 2834 1132 Size: 1702 3774 SspI AAT'ATT Cuts at: 5 52 3400 5 Size: 47 3348 2081 Fragments arranged by size: 3348 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5476 StyI C'CwwG_G Cuts at: 513 1065 2004 2915 513 Size: 552 939 911 3074 Fragments arranged by size: 3074 939 911 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 2572 3503 3520 3673 3858 4303 Size: 1255 458 534 931 17 153 185 445 Cuts at: 4303 325 Size: 1498 Fragments arranged by size: 1498 1255 931 534 458 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3722 5434 96 Size: 274 80 33 51 1443 1745 1712 138 Fragments arranged by size: 1745 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2254 2286 5436 5468 2254 Size: 32 3150 32 2262 Fragments arranged by size: 3150 2262 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5476 XbaI T'CTAG_A Cuts at: 2812 2812 Size: 5476 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2674 2674 Size: 5476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5476 XmaI C'CCGG_G Cuts at: 1870 2801 1870 Size: 931 4545 XmnI GAAnn'nnTTC Cuts at: 3605 3605 Size: 5476 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3284 276 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI