Restriction map for
pFN21AE1415
(Linear) (Six-base) MAPSORT of: KIEE1415.seq Check: 4789 from: 1 to: 771
KIEE1415.orf
With 182 enzymes: SgfI *
August 9, 2016 17:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 288 319 345 460 771
Size: 288 31 26 115 311
Fragments arranged by size:
311 288 115 31 26
AccI GT'mk_AC
Cuts at: 0 171 771
Size: 171 600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 771
Size: 473 298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 300 771
Size: 300 471
AlwNI CAG_nnn'CTG
Cuts at: 0 27 771
Size: 27 744
ApaI G_GGCC'C
Cuts at: 0 344 713 771
Size: 344 369 58
Fragments arranged by size:
369 344 58
AvaI C'yCGr_G
Cuts at: 0 145 771
Size: 145 626
BamHI G'GATC_C
Cuts at: 0 347 771
Size: 347 424
BanI G'GyrC_C
Cuts at: 0 276 771
Size: 276 495
BanII G_rGCy'C
Cuts at: 0 344 713 771
Size: 344 369 58
Fragments arranged by size:
369 344 58
BlpI GC'TnA_GC
Cuts at: 0 5 736 766 771
Size: 5 731 30 5
Fragments arranged by size:
731 30 5 5
Bme1580I G_kGCm'C
Cuts at: 0 344 398 713 771
Size: 344 54 315 58
Fragments arranged by size:
344 315 58 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 771
Size: 198 573
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 771
Size: 310 30 431
Fragments arranged by size:
431 310 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 682 771
Size: 682 89
BseYI C'CCAG_C
Cuts at: 0 645 706 771
Size: 645 61 65
Fragments arranged by size:
645 65 61
BsiHKAI G_wGCw'C
Cuts at: 0 328 771
Size: 328 443
BsmI GAATG_Cn'
Cuts at: 0 192 771
Size: 192 579
Bsp1286I G_dGCh'C
Cuts at: 0 328 344 398 713 771
Size: 328 16 54 315 58
Fragments arranged by size:
328 315 58 54 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 288 319 345 460 771
Size: 288 31 26 115 311
Fragments arranged by size:
311 288 115 31 26
BsrBI CCG'CTC
Cuts at: 0 381 771
Size: 381 390
BsrDI GCAATG_nn'
Cuts at: 0 427 573 771
Size: 427 146 198
Fragments arranged by size:
427 198 146
BstYI r'GATC_y
Cuts at: 0 347 771
Size: 347 424
BtsI GCAGTG_nn'
Cuts at: 0 172 515 771
Size: 172 343 256
Fragments arranged by size:
343 256 172
EaeI y'GGCC_r
Cuts at: 0 618 771
Size: 618 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 473 771
Size: 198 275 298
Fragments arranged by size:
298 275 198
EcoO109I rG'GnC_Cy
Cuts at: 0 142 340 341 710 771
Size: 142 198 1 369 61
Fragments arranged by size:
369 198 142 61 1
FspI TGC'GCA
Cuts at: 0 507 771
Size: 507 264
HincII GTy'rAC
Cuts at: 0 172 771
Size: 172 599
MslI CAynn'nnrTG
Cuts at: 0 227 631 771
Size: 227 404 140
Fragments arranged by size:
404 227 140
MspA1I CmG'CkG
Cuts at: 0 9 24 623 771
Size: 9 15 599 148
Fragments arranged by size:
599 148 15 9
PasI CC'CwG_GG
Cuts at: 0 651 713 771
Size: 651 62 58
Fragments arranged by size:
651 62 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 771
Size: 357 414
PfoI T'CCnGG_A
Cuts at: 0 302 771
Size: 302 469
PspOMI G'GGCC_C
Cuts at: 0 340 709 771
Size: 340 369 62
Fragments arranged by size:
369 340 62
PstI C_TGCA'G
Cuts at: 0 693 771
Size: 693 78
PvuII CAG'CTG
Cuts at: 0 24 771
Size: 24 747
SalI G'TCGA_C
Cuts at: 0 170 771
Size: 170 601
SfcI C'TryA_G
Cuts at: 0 50 603 689 771
Size: 50 553 86 82
Fragments arranged by size:
553 86 82 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 567 771
Size: 33 534 204
Fragments arranged by size:
534 204 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 249 281 771
Size: 249 32 490
Fragments arranged by size:
490 249 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 669 771
Size: 669 102
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaI AvaI BamHI
BanI BanII BlpI Bme1580I BpmI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BstYI BtsI
EaeI Eco57MI EcoO109I FspI HincII MslI MspA1I PasI
PflMI PfoI PspOMI PstI PvuII SalI SfcI TaqII
TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspAI HaeII Hin4I
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI Tth111I XbaI XhoI XmaI
XmnI ZraI