(Circular) (Six-base) MAPSORT of: pFN21AE5212.seq Check: 4387 from: 1 to: 5260 pFN21AE5212 5260 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2445 3070 278 Size: 53 83 186 936 909 625 2468 Fragments arranged by size: 2468 936 909 625 186 83 53 AccI GT'mk_AC Cuts at: 1937 2603 1937 Size: 666 4594 Acc65I G'GTAC_C Cuts at: 2581 2581 Size: 5260 AclI AA'CG_TT Cuts at: 1563 3387 3760 1563 Size: 1824 373 3063 Fragments arranged by size: 3063 1824 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3323 4707 4975 1632 Size: 1691 1384 268 1917 Fragments arranged by size: 1917 1691 1384 268 AfeI AGC'GCT Cuts at: 2302 2302 Size: 5260 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5063 AflIII A'CryG_T Cuts at: 1203 2127 4159 5136 1203 Size: 924 2032 977 1327 Fragments arranged by size: 2032 1327 977 924 AgeI A'CCGG_T Cuts at: 4091 4091 Size: 5260 AhdI GACnn_n'nnGTC Cuts at: 2446 3989 2446 Size: 1543 3717 AleI CACnn'nnGTG Cuts at: 1166 2177 1166 Size: 1011 4249 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5260 AlwNI CAG_nnn'CTG Cuts at: 2067 3003 4575 2067 Size: 936 1572 2752 Fragments arranged by size: 2752 1572 936 ApaLI G'TGCA_C Cuts at: 3317 4473 3317 Size: 1156 4104 ApoI r'AATT_y Cuts at: 1493 2569 2795 2947 5056 1493 Size: 1076 226 152 2109 1697 Fragments arranged by size: 2109 1697 1076 226 152 AseI AT'TA_AT Cuts at: 160 1032 3814 160 Size: 872 2782 1606 Fragments arranged by size: 2782 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5260 AvaI C'yCGr_G Cuts at: 1870 1943 2080 2585 1870 Size: 73 137 505 4545 Fragments arranged by size: 4545 505 137 73 BamHI G'GATC_C Cuts at: 2590 2957 2590 Size: 367 4893 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2581 4036 618 Size: 325 217 627 794 1455 1842 Fragments arranged by size: 1842 1455 794 627 325 217 BanII G_rGCy'C Cuts at: 1391 2015 2025 2579 1391 Size: 624 10 554 4072 Fragments arranged by size: 4072 624 554 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4289 BbvCI CC'TCA_GC Cuts at: 2193 2297 2193 Size: 104 5156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3451 3485 1903 Size: 34 1514 34 3678 Fragments arranged by size: 3678 1514 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3152 4362 3152 Size: 1210 4050 BclI T'GATC_A Cuts at: 1548 1548 Size: 5260 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3871 136 Size: 107 122 71 631 605 2199 1525 Fragments arranged by size: 2199 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5255 5255 Size: 5260 BlpI GC'TnA_GC Cuts at: 2677 2677 Size: 5260 Bme1580I G_kGCm'C Cuts at: 1790 3321 4477 1790 Size: 1531 1156 2573 Fragments arranged by size: 2573 1531 1156 BmgBI CAC'GTC Cuts at: 1318 2215 1318 Size: 897 4363 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2991 3949 442 Size: 416 53 83 399 24 1574 958 1753 Fragments arranged by size: 1753 1574 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5260 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3920 1239 Size: 2681 2579 Bpu10I CC'TnA_GC Cuts at: 2139 2193 2297 2456 2139 Size: 54 104 159 4943 Fragments arranged by size: 4943 159 104 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3385 4250 4548 3385 Size: 865 298 4097 Fragments arranged by size: 4097 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3923 882 Size: 617 2424 2219 Fragments arranged by size: 2424 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2119 3051 493 Size: 711 915 932 2702 Fragments arranged by size: 2702 932 915 711 BsaBI GATnn'nnATC Cuts at: 5254 5254 Size: 5260 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2442 3067 3449 4084 Size: 53 83 186 936 909 625 382 635 Cuts at: 4084 275 Size: 1451 Fragments arranged by size: 1451 936 909 635 625 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3693 4091 4365 4512 4923 3693 Size: 398 274 147 411 4030 Fragments arranged by size: 4030 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2228 2258 2534 2564 1178 Size: 30 165 30 825 30 276 30 3874 Fragments arranged by size: 3874 825 276 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5260 BseYI C'CCAG_C Cuts at: 2508 4463 4959 2508 Size: 1955 496 2809 Fragments arranged by size: 2809 1955 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 1163 Size: 573 315 4372 Fragments arranged by size: 4372 573 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2710 3471 4499 1540 Size: 275 186 709 761 1028 2301 Fragments arranged by size: 2301 1028 761 709 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 2367 3321 3406 4477 2025 Size: 342 954 85 1071 2808 Fragments arranged by size: 2808 1071 954 342 85 BsmI GAATG_Cn' Cuts at: 2771 2864 2771 Size: 93 5167 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2025 2367 2579 3321 3406 4477 Size: 399 225 10 342 212 742 85 1071 Cuts at: 4477 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 342 225 212 85 10 BspEI T'CCGG_A Cuts at: 4923 4923 Size: 5260 BspHI T'CATG_A Cuts at: 2421 3149 4879 2421 Size: 728 1730 2802 Fragments arranged by size: 2802 1730 728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2055 2190 2615 844 Size: 1211 135 425 3489 Fragments arranged by size: 3489 1211 425 135 BsrBI CCG'CTC Cuts at: 1394 1823 2394 3147 1394 Size: 429 571 753 3507 Fragments arranged by size: 3507 753 571 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3755 3929 65 Size: 1183 2507 174 1396 Fragments arranged by size: 2507 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3904 4091 1700 Size: 110 2094 187 2869 Fragments arranged by size: 2869 2094 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5260 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5260 BssSI C'ACGA_G Cuts at: 3320 4332 3320 Size: 1012 4248 BstAPI GCAn_nnn'nTGC Cuts at: 794 2067 794 Size: 1273 3987 BstBI TT'CG_AA Cuts at: 4064 4064 Size: 5260 BstEII G'GTnAC_C Cuts at: 1184 2101 1184 Size: 917 4343 BstYI r'GATC_y Cuts at: 1972 2035 2112 2590 2957 3342 3359 4811 4897 Size: 63 77 478 367 385 17 1452 86 Cuts at: 4897 4909 5255 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 478 385 367 346 86 77 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 2108 513 Size: 552 1043 3665 Fragments arranged by size: 3665 1043 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4521 Fragments arranged by size: 4521 683 56 BtsI GCAGTG_nn' Cuts at: 2371 2776 3570 3590 2371 Size: 405 794 20 4041 Fragments arranged by size: 4041 794 405 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5260 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5225 DraI TTT'AAA Cuts at: 2564 2564 Size: 5260 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4267 809 Size: 507 2951 1802 Fragments arranged by size: 2951 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2356 2707 3596 Size: 54 1425 50 275 18 526 351 889 Cuts at: 3596 5225 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 526 351 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2707 1537 Size: 275 895 4090 Fragments arranged by size: 4090 895 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3190 1353 Size: 1837 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3847 4221 4367 387 Size: 1064 138 2258 374 146 1280 Fragments arranged by size: 2258 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2023 2023 Size: 5260 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3323 3920 4707 4975 1239 Size: 393 1691 597 787 268 1524 Fragments arranged by size: 1691 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2202 2202 Size: 5260 EcoRI G'AATT_C Cuts at: 2569 2947 2569 Size: 378 4882 FspI TGC'GCA Cuts at: 3766 3766 Size: 5260 HaeII r_GCGC'y Cuts at: 1678 2304 4407 1678 Size: 626 2103 2531 Fragments arranged by size: 2531 2103 626 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 2186 2218 2432 Size: 32 135 32 464 32 118 32 214 Cuts at: 2432 2464 3901 3933 3975 4007 1373 Size: 32 1437 32 42 32 2626 Fragments arranged by size: 2626 1437 464 214 135 118 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2218 2604 2850 669 Size: 988 281 280 386 246 3079 Fragments arranged by size: 3079 988 386 281 280 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5260 HpaI GTT'AAC Cuts at: 2850 2850 Size: 5260 KpnI G_GTAC'C Cuts at: 2585 2585 Size: 5260 MfeI C'AATT_G Cuts at: 2859 2859 Size: 5260 MluI A'CGCG_T Cuts at: 2127 5136 2127 Size: 3009 2251 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4374 4558 1305 Size: 3069 184 2007 Fragments arranged by size: 3069 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3438 Fragments arranged by size: 3438 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 3218 3577 3736 518 Size: 648 1011 1041 359 159 2042 Fragments arranged by size: 2042 1041 1011 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2064 2110 2676 3353 4501 4746 1606 Size: 458 46 566 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 566 458 245 46 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4708 NdeI CA'TA_TG Cuts at: 387 387 Size: 5260 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5260 NotI GC'GGCC_GC Cuts at: 2707 2707 Size: 5260 NspI r_CATG'y Cuts at: 1132 1229 2618 4163 1132 Size: 97 1389 1545 2229 Fragments arranged by size: 2229 1545 1389 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5260 PciI A'CATG_T Cuts at: 4159 4159 Size: 5260 PflMI CCAn_nnn'nTGG Cuts at: 1108 2341 1108 Size: 1233 4027 PmeI GTTT'AAAC Cuts at: 2564 2564 Size: 5260 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3329 3361 4842 4874 3329 Size: 32 1481 32 3715 Fragments arranged by size: 3715 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2202 2202 Size: 5260 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5260 PsiI TTA'TAA Cuts at: 2830 2830 Size: 5260 PstI C_TGCA'G Cuts at: 830 2160 2612 830 Size: 1330 452 3478 Fragments arranged by size: 3478 1330 452 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5260 SacI G_AGCT'C Cuts at: 2025 2025 Size: 5260 SacII CC_GC'GG Cuts at: 2111 2111 Size: 5260 SalI G'TCGA_C Cuts at: 1936 2602 1936 Size: 666 4594 SbfI CC_TGCA'GG Cuts at: 2612 2612 Size: 5260 ScaI AGT'ACT Cuts at: 3508 5220 3508 Size: 1712 3548 SfcI C'TryA_G Cuts at: 826 1045 2071 2156 2608 3743 4424 4615 826 Size: 219 1026 85 452 1135 681 191 1471 Fragments arranged by size: 1471 1135 1026 681 452 219 191 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5260 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5260 SmaI CCC'GGG Cuts at: 1872 2587 1872 Size: 715 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3364 4265 4527 820 Size: 197 926 1421 901 262 1553 Fragments arranged by size: 1553 1421 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3051 493 Size: 2558 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5260 SphI G_CATG'C Cuts at: 1132 2618 1132 Size: 1486 3774 SspI AAT'ATT Cuts at: 5 52 3184 5 Size: 47 3132 2081 Fragments arranged by size: 3132 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5260 StyI C'CwwG_G Cuts at: 513 1065 2538 2699 513 Size: 552 1473 161 3074 Fragments arranged by size: 3074 1473 552 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 3287 3304 3457 3642 4087 325 Size: 1255 609 1098 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1098 609 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3506 5218 96 Size: 274 80 33 51 1443 1529 1712 138 Fragments arranged by size: 1712 1529 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5220 5252 5220 Size: 32 5228 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5260 XbaI T'CTAG_A Cuts at: 2596 2596 Size: 5260 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5260 XmaI C'CCGG_G Cuts at: 1870 2585 1870 Size: 715 4545 XmnI GAAnn'nnTTC Cuts at: 3389 3389 Size: 5260 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2443 3068 276 Size: 53 83 186 936 909 625 2468 Fragments arranged by size: 2468 936 909 625 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI