Restriction map for pFN21AE5212
 (Linear) (Six-base) MAPSORT of: KIEE5212.seq  Check: 1679  from: 1  to: 555



KIEE5212.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:51  ..

AatII G_ACGT'C

Cuts at:      0     440     555 
   Size:        440     115
 
AfeI AGC'GCT

Cuts at:      0     297     555 
   Size:        297     258
 
AflIII A'CryG_T

Cuts at:      0     122     555 
   Size:        122     433
 
AhdI GACnn_n'nnGTC

Cuts at:      0     441     555 
   Size:        441     114
 
AleI CACnn'nnGTG

Cuts at:      0     172     555 
   Size:        172     383
 
AlwNI CAG_nnn'CTG

Cuts at:      0      62     555 
   Size:         62     493
 
AvaI C'yCGr_G

Cuts at:      0      75     555 
   Size:         75     480
 
BanII G_rGCy'C

Cuts at:      0      10      20     555 
   Size:         10      10     535

  Fragments arranged by size: 

                 535      10      10
 
BbvCI CC'TCA_GC

Cuts at:      0     188     292     555 
   Size:        188     104     263

  Fragments arranged by size: 

                 263     188     104
 
BmgBI CAC'GTC

Cuts at:      0     210     555 
   Size:        210     345
 
Bpu10I CC'TnA_GC

Cuts at:      0     134     188     292     451     555 
   Size:        134      54     104     159     104

  Fragments arranged by size: 

                 159     134     104     104      54
 
BsaAI yAC'GTr

Cuts at:      0     114     555 
   Size:        114     441
 
BsaHI Gr'CG_yC

Cuts at:      0     437     555 
   Size:        437     118
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     223     253     529     555 
   Size:        223      30     276      26

  Fragments arranged by size: 

                 276     223      30      26
 
BseYI C'CCAG_C

Cuts at:      0     503     555 
   Size:        503      52
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     555 
   Size:         46     509
 
BsiHKAI G_wGCw'C

Cuts at:      0      20     362     555 
   Size:         20     342     193

  Fragments arranged by size: 

                 342     193      20
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      20     362     555 
   Size:         10      10     342     193

  Fragments arranged by size: 

                 342     193      10      10
 
BspHI T'CATG_A

Cuts at:      0     416     555 
   Size:        416     139
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      50     185     555 
   Size:         50     135     370

  Fragments arranged by size: 

                 370     135      50
 
BsrBI CCG'CTC

Cuts at:      0     389     555 
   Size:        389     166
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      62     555 
   Size:         62     493
 
BstEII G'GTnAC_C

Cuts at:      0      96     555 
   Size:         96     459
 
BstYI r'GATC_y

Cuts at:      0      30     107     555 
   Size:         30      77     448

  Fragments arranged by size: 

                 448      77      30
 
BtgI C'CryG_G

Cuts at:      0     103     555 
   Size:        103     452
 
BtsI GCAGTG_nn'

Cuts at:      0     366     555 
   Size:        366     189
 
EaeI y'GGCC_r

Cuts at:      0     351     555 
   Size:        351     204
 
EcoICRI GAG'CTC

Cuts at:      0      18     555 
   Size:         18     537
 
EcoO109I rG'GnC_Cy

Cuts at:      0     197     555 
   Size:        197     358
 
HaeII r_GCGC'y

Cuts at:      0     299     555 
   Size:        299     256
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      31      63     181     213     427     459     555 
   Size:         31      32     118      32     214      32      96

  Fragments arranged by size: 

                 214     118      96      32      32      32      31
 
HincII GTy'rAC

Cuts at:      0     213     555 
   Size:        213     342
 
MluI A'CGCG_T

Cuts at:      0     122     555 
   Size:        122     433
 
MslI CAynn'nnrTG

Cuts at:      0     172     555 
   Size:        172     383
 
MspA1I CmG'CkG

Cuts at:      0      59     105     555 
   Size:         59      46     450

  Fragments arranged by size: 

                 450      59      46
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     336     555 
   Size:        336     219
 
PpuMI rG'GwC_Cy

Cuts at:      0     197     555 
   Size:        197     358
 
PstI C_TGCA'G

Cuts at:      0     155     555 
   Size:        155     400
 
SacI G_AGCT'C

Cuts at:      0      20     555 
   Size:         20     535
 
SacII CC_GC'GG

Cuts at:      0     106     555 
   Size:        106     449
 
SfcI C'TryA_G

Cuts at:      0      66     151     555 
   Size:         66      85     404

  Fragments arranged by size: 

                 404      85      66
 
StyI C'CwwG_G

Cuts at:      0     533     555 
   Size:        533      22
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     184     555 
   Size:        184     371
 
ZraI GAC'GTC

Cuts at:      0     438     555 
   Size:        438     117
 

 Enzymes that do cut:

    AatII     AfeI   AflIII     AhdI     AleI    AlwNI     AvaI    BanII
    BbvCI    BmgBI   Bpu10I    BsaAI    BsaHI    BsaXI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI   BstAPI   BstEII    BstYI
     BtgI     BtsI     EaeI  EcoICRI EcoO109I    HaeII    Hin4I   HincII
     MluI     MslI   MspA1I    PflMI    PpuMI     PstI     SacI    SacII
     SfcI     StyI    TaqII     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaBI    BsaWI    BseRI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
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