(Linear) (Six-base) MAPSORT of: KIEE5212.seq Check: 1679 from: 1 to: 555 KIEE5212.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. AatII G_ACGT'C Cuts at: 0 440 555 Size: 440 115 AfeI AGC'GCT Cuts at: 0 297 555 Size: 297 258 AflIII A'CryG_T Cuts at: 0 122 555 Size: 122 433 AhdI GACnn_n'nnGTC Cuts at: 0 441 555 Size: 441 114 AleI CACnn'nnGTG Cuts at: 0 172 555 Size: 172 383 AlwNI CAG_nnn'CTG Cuts at: 0 62 555 Size: 62 493 AvaI C'yCGr_G Cuts at: 0 75 555 Size: 75 480 BanII G_rGCy'C Cuts at: 0 10 20 555 Size: 10 10 535 Fragments arranged by size: 535 10 10 BbvCI CC'TCA_GC Cuts at: 0 188 292 555 Size: 188 104 263 Fragments arranged by size: 263 188 104 BmgBI CAC'GTC Cuts at: 0 210 555 Size: 210 345 Bpu10I CC'TnA_GC Cuts at: 0 134 188 292 451 555 Size: 134 54 104 159 104 Fragments arranged by size: 159 134 104 104 54 BsaAI yAC'GTr Cuts at: 0 114 555 Size: 114 441 BsaHI Gr'CG_yC Cuts at: 0 437 555 Size: 437 118 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 223 253 529 555 Size: 223 30 276 26 Fragments arranged by size: 276 223 30 26 BseYI C'CCAG_C Cuts at: 0 503 555 Size: 503 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 555 Size: 46 509 BsiHKAI G_wGCw'C Cuts at: 0 20 362 555 Size: 20 342 193 Fragments arranged by size: 342 193 20 Bsp1286I G_dGCh'C Cuts at: 0 10 20 362 555 Size: 10 10 342 193 Fragments arranged by size: 342 193 10 10 BspHI T'CATG_A Cuts at: 0 416 555 Size: 416 139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 50 185 555 Size: 50 135 370 Fragments arranged by size: 370 135 50 BsrBI CCG'CTC Cuts at: 0 389 555 Size: 389 166 BstAPI GCAn_nnn'nTGC Cuts at: 0 62 555 Size: 62 493 BstEII G'GTnAC_C Cuts at: 0 96 555 Size: 96 459 BstYI r'GATC_y Cuts at: 0 30 107 555 Size: 30 77 448 Fragments arranged by size: 448 77 30 BtgI C'CryG_G Cuts at: 0 103 555 Size: 103 452 BtsI GCAGTG_nn' Cuts at: 0 366 555 Size: 366 189 EaeI y'GGCC_r Cuts at: 0 351 555 Size: 351 204 EcoICRI GAG'CTC Cuts at: 0 18 555 Size: 18 537 EcoO109I rG'GnC_Cy Cuts at: 0 197 555 Size: 197 358 HaeII r_GCGC'y Cuts at: 0 299 555 Size: 299 256 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 31 63 181 213 427 459 555 Size: 31 32 118 32 214 32 96 Fragments arranged by size: 214 118 96 32 32 32 31 HincII GTy'rAC Cuts at: 0 213 555 Size: 213 342 MluI A'CGCG_T Cuts at: 0 122 555 Size: 122 433 MslI CAynn'nnrTG Cuts at: 0 172 555 Size: 172 383 MspA1I CmG'CkG Cuts at: 0 59 105 555 Size: 59 46 450 Fragments arranged by size: 450 59 46 PflMI CCAn_nnn'nTGG Cuts at: 0 336 555 Size: 336 219 PpuMI rG'GwC_Cy Cuts at: 0 197 555 Size: 197 358 PstI C_TGCA'G Cuts at: 0 155 555 Size: 155 400 SacI G_AGCT'C Cuts at: 0 20 555 Size: 20 535 SacII CC_GC'GG Cuts at: 0 106 555 Size: 106 449 SfcI C'TryA_G Cuts at: 0 66 151 555 Size: 66 85 404 Fragments arranged by size: 404 85 66 StyI C'CwwG_G Cuts at: 0 533 555 Size: 533 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 184 555 Size: 184 371 ZraI GAC'GTC Cuts at: 0 438 555 Size: 438 117 Enzymes that do cut: AatII AfeI AflIII AhdI AleI AlwNI AvaI BanII BbvCI BmgBI Bpu10I BsaAI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BstAPI BstEII BstYI BtgI BtsI EaeI EcoICRI EcoO109I HaeII Hin4I HincII MluI MslI MspA1I PflMI PpuMI PstI SacI SacII SfcI StyI TaqII ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AflII AgeI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI