################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BbsI BbvCI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspEI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII Cfr10I DraI DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FseI HincII HindII HpaI KasI KpnI MaeI MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI SalI SapI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 145 GTCTAC GT^[AC][GT]AC AcyI 883 GACGCC G[GA]^CG[CT]C Asp700I 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 749 TGCGCA TGC^GCA BcgI 1013 GCAGCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1255 GTATCC GTATCC(6/5) BclI 68 TGATCA T^GATCA BpmI 130 CTGGAG CTGGAG(16/14) BsaAI 106 TACGTG [CT]AC^GT[GA] BsaHI 883 GACGCC G[GA]^CG[CT]C BsaI 592 GAGACC GGTCTC(1/5) BsiEI 95 CGGCCG CG[GA][CT]^CG BsmBI 164 CGTCTC CGTCTC(1/5) BsmFI 668 GGGAC GGGAC(10/14) BspDI 1330 ATCGAT AT^CGAT BspHI 488 TCATGA T^CATGA BspMI 746 ACCTGC ACCTGC(4/8) BsrBI 814 CCGCTC CCGCTC(-3/-3) BstEII 705 GGTCACC G^GT[ACGT]ACC BstPI 705 GGTCACC G^GT[ACGT]ACC BstYI 461 AGATCC [GA]^GATC[CT] ClaI 1330 ATCGAT AT^CGAT CpoI 479 CGGACCG CG^G[AT]CCG DraIII 679 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 95 CGGCCG C^GGCCG EarI 1321 GAAGAG CTCTTC(1/4) EclXI 95 CGGCCG C^GGCCG Eco52I 95 CGGCCG C^GGCCG EcoO65I 705 GGTCACC G^GT[ACGT]ACC FbaI 68 TGATCA T^GATCA FspI 749 TGCGCA TGC^GCA Hin1I 883 GACGCC G[GA]^CG[CT]C HindIII 1304 AAGCTT A^AGCTT Ksp632I 1321 GAAGAG CTCTTC(1/4) MaeIII 706 GTCAC ^GT[ACGT]AC MflI 461 AGATCC [GA]^GATC[CT] PpuMI 668 GGGACCT [GA]G^G[AT]CC[CT] RcaI 488 TCATGA T^CATGA RsrII 479 CGGACCG CG^G[AT]CCG SacI 862 GAGCTC GAGCT^C SbfI 387 CCTGCAGG CCTGCA^GG SmaI 726 CCCGGG CCC^GGG Sse8387I 387 CCTGCAGG CCTGCA^GG StuI 1175 AGGCCT AGG^CCT Tsp45I 706 GTCAC ^GT[GC]AC XhoII 461 AGATCC [GA]^GATC[CT] XmaCI 726 CCCGGG C^CCGGG XmaI 726 CCCGGG C^CCGGG XmnI 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Alw44I 13 GTGCAC G^TGCAC 565 GTGCAC AlwI 462 GATCC GGATC(4/5) 910 GATCC AlwNI 985 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 1123 CAGGAGCTG ApaI 845 GGGCCC GGGCC^C 955 GGGCCC ApaLI 13 GTGCAC G^TGCAC 565 GTGCAC AvaI 726 CCCGGG C^[CT]CG[GA]G 859 CCCGAG AvaII 480 GGACC G^G[AT]CC 669 GGACC BglI 237 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 386 GCCTGCAGGGC BseRI 469 GAGGAG GAGGAG(10/8) 1324 GAGGAG BsoBI 726 CCCGGG C^[CT]CG[GA]G 859 CCCGAG DraII 668 GGGACCT [GA]G^G[ACGT]CC[CT] 845 GGGCCCT EcoO109I 668 GGGACCT [GA]G^G[ACGT]CC[CT] 845 GGGCCCT HaeII 120 GGCGCT [GA]GCGC^[CT] 615 GGCGCT MslI 531 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 960 CATGTCCATG NcoI 215 CCATGG C^CATGG 918 CCATGG PflMI 53 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 570 CCAGCTGGTGG PleI 115 GACTC GAGTC(4/5) 157 GAGTC PstI 124 CTGCAG CTGCA^G 388 CTGCAG PvuII 127 CAGCTG CAG^CTG 571 CAGCTG SexAI 680 ACCTGGT A^CC[AT]GGT 1250 ACCTGGT SfcI 124 CTGCAG C^T[GA][CT]AG 388 CTGCAG TfiI 137 GAATC G^A[AT]TC 1032 GATTC Van91I 53 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 570 CCAGCTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AfaI AluI AspHI BalI BanI BanII BbvI BcnI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KspI MaeII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI SacII Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI TspRI TthHB8I XcmI