(Linear) (Six-base) MAPSORT of: KIEE1530.seq Check: 5120 from: 1 to: 1338 KIEE1530.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AccI GT'mk_AC Cuts at: 0 146 1338 Size: 146 1192 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 483 664 1102 1338 Size: 483 181 438 236 Fragments arranged by size: 483 438 236 181 AleI CACnn'nnGTG Cuts at: 0 535 1338 Size: 535 803 AlwNI CAG_nnn'CTG Cuts at: 0 990 1128 1338 Size: 990 138 210 Fragments arranged by size: 990 210 138 ApaI G_GGCC'C Cuts at: 0 849 959 1338 Size: 849 110 379 Fragments arranged by size: 849 379 110 ApaLI G'TGCA_C Cuts at: 0 13 565 1338 Size: 13 552 773 Fragments arranged by size: 773 552 13 AvaI C'yCGr_G Cuts at: 0 726 859 1338 Size: 726 133 479 Fragments arranged by size: 726 479 133 BanI G'GyrC_C Cuts at: 0 49 211 771 1338 Size: 49 162 560 567 Fragments arranged by size: 567 560 162 49 BanII G_rGCy'C Cuts at: 0 433 545 849 866 959 1338 Size: 433 112 304 17 93 379 Fragments arranged by size: 433 379 304 112 93 17 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1002 1036 1338 Size: 1002 34 302 Fragments arranged by size: 1002 302 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1266 1338 Size: 1266 72 BclI T'GATC_A Cuts at: 0 68 1338 Size: 68 1270 BglI GCCn_nnn'nGGC Cuts at: 0 243 392 1338 Size: 243 149 946 Fragments arranged by size: 946 243 149 Bme1580I G_kGCm'C Cuts at: 0 17 182 314 445 569 776 849 860 Size: 17 165 132 131 124 207 73 11 Cuts at: 860 959 1338 Size: 99 379 Fragments arranged by size: 379 207 165 132 131 124 99 73 17 11 BmgBI CAC'GTC Cuts at: 0 354 1338 Size: 354 984 BmrI ACTGGGnnnn_n' Cuts at: 0 309 1338 Size: 309 1029 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1170 1338 Size: 1170 168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 1338 Size: 151 1187 Bpu10I CC'TnA_GC Cuts at: 0 432 1338 Size: 432 906 BsaI GGTCTCn'nnnn_ Cuts at: 0 586 1338 Size: 586 752 BsaAI yAC'GTr Cuts at: 0 108 1338 Size: 108 1230 BsaHI Gr'CG_yC Cuts at: 0 884 1338 Size: 884 454 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 92 122 1338 Size: 92 30 1216 Fragments arranged by size: 1216 92 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 484 1338 Size: 484 854 BseYI C'CCAG_C Cuts at: 0 414 869 1338 Size: 414 455 469 Fragments arranged by size: 469 455 414 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 308 772 1168 1338 Size: 308 464 396 170 Fragments arranged by size: 464 396 308 170 BsiEI CG_ry'CG Cuts at: 0 98 1338 Size: 98 1240 BsiHKAI G_wGCw'C Cuts at: 0 17 83 377 569 866 1338 Size: 17 66 294 192 297 472 Fragments arranged by size: 472 297 294 192 66 17 BsmBI CGTCTCn'nnnn_ Cuts at: 0 170 1338 Size: 170 1168 Bsp1286I G_dGCh'C Cuts at: 0 17 83 182 314 377 433 445 545 Size: 17 66 99 132 63 56 12 100 Cuts at: 545 569 776 849 860 866 959 1338 Size: 24 207 73 11 6 93 379 Fragments arranged by size: 379 207 132 100 99 93 73 66 63 56 24 17 12 11 6 BspHI T'CATG_A Cuts at: 0 488 1338 Size: 488 850 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 755 1338 Size: 755 583 BsrBI CCG'CTC Cuts at: 0 816 1338 Size: 816 522 BstEII G'GTnAC_C Cuts at: 0 705 1338 Size: 705 633 BstYI r'GATC_y Cuts at: 0 461 1338 Size: 461 877 BtgI C'CryG_G Cuts at: 0 215 825 918 935 951 1074 1338 Size: 215 610 93 17 16 123 264 Fragments arranged by size: 610 264 215 123 93 17 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 89 1338 Size: 89 1249 BtsI GCAGTG_nn' Cuts at: 0 350 375 1338 Size: 350 25 963 Fragments arranged by size: 963 350 25 ClaI AT'CG_AT Cuts at: 0 1331 1338 Size: 1331 7 DraIII CAC_nnn'GTG Cuts at: 0 684 1338 Size: 684 654 EaeI y'GGCC_r Cuts at: 0 95 276 899 921 938 992 1052 1115 Size: 95 181 623 22 17 54 60 63 Cuts at: 1115 1338 Size: 223 Fragments arranged by size: 623 223 181 95 63 60 54 22 17 EagI C'GGCC_G Cuts at: 0 95 1338 Size: 95 1243 EarI CTCTTCn'nnn_ Cuts at: 0 1316 1338 Size: 1316 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 678 1246 1338 Size: 678 568 92 Fragments arranged by size: 678 568 92 EcoICRI GAG'CTC Cuts at: 0 864 1338 Size: 864 474 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 483 664 1102 1338 Size: 151 332 181 438 236 Fragments arranged by size: 438 332 236 181 151 EcoO109I rG'GnC_Cy Cuts at: 0 669 846 1338 Size: 669 177 492 Fragments arranged by size: 669 492 177 FspI TGC'GCA Cuts at: 0 751 1338 Size: 751 587 HaeII r_GCGC'y Cuts at: 0 124 619 1338 Size: 124 495 719 Fragments arranged by size: 719 495 124 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 148 180 1240 1272 1338 Size: 148 32 1060 32 66 Fragments arranged by size: 1060 148 66 32 32 HindIII A'AGCT_T Cuts at: 0 1304 1338 Size: 1304 34 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 506 1338 Size: 506 832 MscI TGG'CCA Cuts at: 0 278 940 994 1338 Size: 278 662 54 344 Fragments arranged by size: 662 344 278 54 MslI CAynn'nnrTG Cuts at: 0 535 964 1338 Size: 535 429 374 Fragments arranged by size: 535 429 374 MspA1I CmG'CkG Cuts at: 0 129 573 827 953 1076 1338 Size: 129 444 254 126 123 262 Fragments arranged by size: 444 262 254 129 126 123 NcoI C'CATG_G Cuts at: 0 215 918 1338 Size: 215 703 420 Fragments arranged by size: 703 420 215 PasI CC'CwG_GG Cuts at: 0 182 1338 Size: 182 1156 PflMI CCAn_nnn'nTGG Cuts at: 0 59 576 1338 Size: 59 517 762 Fragments arranged by size: 762 517 59 PfoI T'CCnGG_A Cuts at: 0 476 1121 1338 Size: 476 645 217 Fragments arranged by size: 645 476 217 PpuMI rG'GwC_Cy Cuts at: 0 669 1338 Size: 669 669 PspOMI G'GGCC_C Cuts at: 0 845 955 1338 Size: 845 110 383 Fragments arranged by size: 845 383 110 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1000 1032 1338 Size: 1000 32 306 Fragments arranged by size: 1000 306 32 PstI C_TGCA'G Cuts at: 0 128 392 1338 Size: 128 264 946 Fragments arranged by size: 946 264 128 PvuII CAG'CTG Cuts at: 0 129 573 1338 Size: 129 444 765 Fragments arranged by size: 765 444 129 RsrII CG'GwC_CG Cuts at: 0 480 1338 Size: 480 858 SacI G_AGCT'C Cuts at: 0 866 1338 Size: 866 472 SacII CC_GC'GG Cuts at: 0 828 954 1077 1338 Size: 828 126 123 261 Fragments arranged by size: 828 261 126 123 SbfI CC_TGCA'GG Cuts at: 0 392 1338 Size: 392 946 SexAI A'CCwGG_T Cuts at: 0 680 1250 1338 Size: 680 570 88 Fragments arranged by size: 680 570 88 SfcI C'TryA_G Cuts at: 0 124 388 1338 Size: 124 264 950 Fragments arranged by size: 950 264 124 SmaI CCC'GGG Cuts at: 0 728 1338 Size: 728 610 StuI AGG'CCT Cuts at: 0 1177 1338 Size: 1177 161 StyI C'CwwG_G Cuts at: 0 215 518 918 1338 Size: 215 303 400 420 Fragments arranged by size: 420 400 303 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 883 1338 Size: 883 455 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 92 124 670 702 1338 Size: 92 32 546 32 636 Fragments arranged by size: 636 546 92 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 189 894 972 1338 Size: 189 705 78 366 Fragments arranged by size: 705 366 189 78 XmaI C'CCGG_G Cuts at: 0 726 1338 Size: 726 612 XmnI GAAnn'nnTTC Cuts at: 0 496 1338 Size: 496 842 Enzymes that do cut: AccI AcuI AleI AlwNI ApaI ApaLI AvaI BanI BanII BcgI BciVI BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BstEII BstYI BtgI BtgZI BtsI ClaI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI PasI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII RsrII SacI SacII SbfI SexAI SfcI SmaI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BfrBI BglII BlpI BmtI BpuEI BsaBI BsaWI BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I CspCI DraI DrdI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PvuI SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI ZraI