Restriction map for
pFN21AE3615
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BanI
BbeI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TaqI Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 140 CGCG CG^CG
AhdI 648 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 268 GTGCAC G^TGCAC
AlwNI 775 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 268 GTGCAC G^TGCAC
AspEI 648 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 268 GTGCAC G[AT]GC[AT]^C
AvaI 480 CTCGGG C^[CT]CG[GA]G
AvaII 647 GGACC G^G[AT]CC
BanII 729 GGGCTC G[GA]GC[CT]^C
BbrPI 492 CACGTG CAC^GTG
BbsI 528 GAAGAC GAAGAC(2/6)
BcnI 408 CCGGG CC^[GC]GG
BfaI 109 CTAG C^TAG
BpuAI 528 GAAGAC GAAGAC(2/6)
BsaAI 492 CACGTG [CT]AC^GT[GA]
BseRI 288 CTCCTC GAGGAG(10/8)
BsgI 723 CTGCAC GTGCAG(16/14)
BsiHKAI 268 GTGCAC G[AT]GC[AT]^C
BsoBI 480 CTCGGG C^[CT]CG[GA]G
BspHI 299 TCATGA T^CATGA
BspLU11I 356 ACATGT A^CATGT
BstAPI 803 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 140 CGCG CG^CG
CfoI 285 GCGC GCG^C
DraIII 489 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 824 CCGTGG C^C[GA][CT]GG
Eam1105I 648 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 592 CTCTTC CTCTTC(1/4)
FauI 765 GCGGG CCCGC(4/6)
HapII 408 CCGG C^CGG
HhaI 285 GCGC GCG^C
HinP1I 285 GCGC G^CGC
HpaII 408 CCGG C^CGG
Ksp632I 592 CTCTTC CTCTTC(1/4)
MaeI 109 CTAG C^TAG
MseI 151 TTAA T^TAA
MspA1I 962 CAGCTG C[AC]G^C[GT]G
MspI 408 CCGG C^CGG
MvnI 140 CGCG CG^CG
NciI 408 CCGGG CC^[GC]GG
PmaCI 492 CACGTG CAC^GTG
PmlI 492 CACGTG CAC^GTG
PpuMI 646 GGGACCT [GA]G^G[AT]CC[CT]
PstI 810 CTGCAG CTGCA^G
PvuII 962 CAGCTG CAG^CTG
RcaI 299 TCATGA T^CATGA
SfcI 810 CTGCAG C^T[GA][CT]AG
SmlI 40 CTCAAG C^T[CT][GA]AG
SspI 466 AATATT AAT^ATT
TfiI 196 GATTC G^A[AT]TC
Tru9I 151 TTAA T^TAA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AflIII 356 ACATGT A^C[GA][CT]GT
493 ACGTGT
BmyI 268 GTGCAC G[AGT]GC[ACT]^C
729 GGGCTC
BsmAI 514 GAGAC GTCTC(1/5)
576 GTCTC
Bsp1286I 268 GTGCAC G[AGT]GC[ACT]^C
729 GGGCTC
BspMI 649 ACCTGC ACCTGC(4/8)
761 ACCTGC
BsrDI 392 GCAATG GCAATG(2/0)
881 GCAATG
BssSI 673 CACGAG CACGAG(-5/-1)
796 CACGAG
DpnII 237 GATC ^GATC
783 GATC
DraII 604 GGGGCCT [GA]G^G[ACGT]CC[CT]
646 GGGACCT
EcoO109I 604 GGGGCCT [GA]G^G[ACGT]CC[CT]
646 GGGACCT
HphI 553 GGTGA GGTGA(8/7)
942 GGTGA
MboI 237 GATC ^GATC
783 GATC
MslI 547 CACACGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
819 CACGTCCGTG
NdeII 237 GATC ^GATC
783 GATC
NspI 337 GCATGT [GA]CATG^[CT]
356 ACATGT
PflMI 179 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
623 CCAAGCTCTGG
Sau3AI 237 GATC ^GATC
783 GATC
Van91I 179 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
623 CCAAGCTCTGG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BalI BbvI BsaJI
BsiYI BslI BsmFI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EaeI EcoRII Fnu4HI FokI HaeIII
HgaI HinfI ItaI MaeII MaeIII
MboII MluNI MnlI MscI MvaI
MwoI NlaIII NlaIV PleI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI