Restriction map for
pFN21AE1303
(Linear) (Six-base) MAPSORT of: KIEE1303.seq Check: 501 from: 1 to: 1248
KIEE1303.orf
With 182 enzymes: SgfI *
May 19, 2017 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 51 737 1248
Size: 51 686 511
Fragments arranged by size:
686 511 51
AccI GT'mk_AC
Cuts at: 0 749 1248
Size: 749 499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1151 1248
Size: 929 222 97
Fragments arranged by size:
929 222 97
AhdI GACnn_n'nnGTC
Cuts at: 0 1140 1248
Size: 1140 108
AlwNI CAG_nnn'CTG
Cuts at: 0 142 745 1248
Size: 142 603 503
Fragments arranged by size:
603 503 142
AvaI C'yCGr_G
Cuts at: 0 191 934 1248
Size: 191 743 314
Fragments arranged by size:
743 314 191
BanI G'GyrC_C
Cuts at: 0 32 1248
Size: 32 1216
BanII G_rGCy'C
Cuts at: 0 476 530 1125 1248
Size: 476 54 595 123
Fragments arranged by size:
595 476 123 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 917 1139 1248
Size: 917 222 109
Fragments arranged by size:
917 222 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 275 309 604 638 925 959 1248
Size: 275 34 295 34 287 34 289
Fragments arranged by size:
295 289 287 275 34 34 34
BclI T'GATC_A
Cuts at: 0 173 1248
Size: 173 1075
BglII A'GATC_T
Cuts at: 0 180 1248
Size: 180 1068
BlpI GC'TnA_GC
Cuts at: 0 88 1248
Size: 88 1160
BmrI ACTGGGnnnn_n'
Cuts at: 0 316 1007 1248
Size: 316 691 241
Fragments arranged by size:
691 316 241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 221 912 1248
Size: 221 691 336
Fragments arranged by size:
691 336 221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 419 670 1080 1248
Size: 205 214 251 410 168
Fragments arranged by size:
410 251 214 205 168
Bpu10I CC'TnA_GC
Cuts at: 0 463 1153 1248
Size: 463 690 95
Fragments arranged by size:
690 463 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1248
Size: 445 803
BsaI GGTCTCn'nnnn_
Cuts at: 0 1087 1248
Size: 1087 161
BsaHI Gr'CG_yC
Cuts at: 0 80 573 989 1248
Size: 80 493 416 259
Fragments arranged by size:
493 416 259 80
BsaWI w'CCGG_w
Cuts at: 0 366 1220 1248
Size: 366 854 28
Fragments arranged by size:
854 366 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 573 1248
Size: 573 675
BseYI C'CCAG_C
Cuts at: 0 817 1248
Size: 817 431
BsiEI CG_ry'CG
Cuts at: 0 779 1248
Size: 779 469
BsiHKAI G_wGCw'C
Cuts at: 0 332 423 678 1248
Size: 332 91 255 570
Fragments arranged by size:
570 332 255 91
Bsp1286I G_dGCh'C
Cuts at: 0 332 423 476 530 678 1125 1248
Size: 332 91 53 54 148 447 123
Fragments arranged by size:
447 332 148 123 91 54 53
BspEI T'CCGG_A
Cuts at: 0 1220 1248
Size: 1220 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 51 737 1145 1248
Size: 51 686 408 103
Fragments arranged by size:
686 408 103 51
BsrFI r'CCGG_y
Cuts at: 0 35 1248
Size: 35 1213
BstXI CCAn_nnnn'nTGG
Cuts at: 0 384 633 859 1248
Size: 384 249 226 389
Fragments arranged by size:
389 384 249 226
BstYI r'GATC_y
Cuts at: 0 145 180 1248
Size: 145 35 1068
Fragments arranged by size:
1068 145 35
BtgI C'CryG_G
Cuts at: 0 669 879 1248
Size: 669 210 369
Fragments arranged by size:
669 369 210
BtsI GCAGTG_nn'
Cuts at: 0 483 1123 1248
Size: 483 640 125
Fragments arranged by size:
640 483 125
DraI TTT'AAA
Cuts at: 0 1239 1248
Size: 1239 9
DraIII CAC_nnn'GTG
Cuts at: 0 1015 1248
Size: 1015 233
EaeI y'GGCC_r
Cuts at: 0 480 1248
Size: 480 768
EarI CTCTTCn'nnn_
Cuts at: 0 47 212 1248
Size: 47 165 1036
Fragments arranged by size:
1036 165 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 419 670 929 1080 1151 1248
Size: 205 214 251 259 151 71 97
Fragments arranged by size:
259 251 214 205 151 97 71
EcoO109I rG'GnC_Cy
Cuts at: 0 107 971 1065 1248
Size: 107 864 94 183
Fragments arranged by size:
864 183 107 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 165 197 979 1011 1126 1158 1248
Size: 165 32 782 32 115 32 90
Fragments arranged by size:
782 165 115 90 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1248
Size: 572 676
MscI TGG'CCA
Cuts at: 0 482 1248
Size: 482 766
MslI CAynn'nnrTG
Cuts at: 0 1024 1248
Size: 1024 224
MspA1I CmG'CkG
Cuts at: 0 132 171 812 984 1248
Size: 132 39 641 172 264
Fragments arranged by size:
641 264 172 132 39
NaeI GCC'GGC
Cuts at: 0 37 1248
Size: 37 1211
NcoI C'CATG_G
Cuts at: 0 879 1248
Size: 879 369
NdeI CA'TA_TG
Cuts at: 0 113 1248
Size: 113 1135
NgoMIV G'CCGG_C
Cuts at: 0 35 1248
Size: 35 1213
NspI r_CATG'y
Cuts at: 0 252 1248
Size: 252 996
PasI CC'CwG_GG
Cuts at: 0 739 1248
Size: 739 509
PfoI T'CCnGG_A
Cuts at: 0 956 1057 1248
Size: 956 101 191
Fragments arranged by size:
956 191 101
PpuMI rG'GwC_Cy
Cuts at: 0 971 1065 1248
Size: 971 94 183
Fragments arranged by size:
971 183 94
PsiI TTA'TAA
Cuts at: 0 1190 1248
Size: 1190 58
PspXI vC'TCGA_Gb
Cuts at: 0 191 1248
Size: 191 1057
PstI C_TGCA'G
Cuts at: 0 62 734 1142 1248
Size: 62 672 408 106
Fragments arranged by size:
672 408 106 62
PvuII CAG'CTG
Cuts at: 0 171 812 984 1248
Size: 171 641 172 264
Fragments arranged by size:
641 264 172 171
SanDI GG'GwC_CC
Cuts at: 0 1065 1248
Size: 1065 183
SapI GCTCTTCn'nnn_
Cuts at: 0 47 212 1248
Size: 47 165 1036
Fragments arranged by size:
1036 165 47
SfcI C'TryA_G
Cuts at: 0 58 730 1138 1248
Size: 58 672 408 110
Fragments arranged by size:
672 408 110 58
SmlI C'TyrA_G
Cuts at: 0 191 424 1248
Size: 191 233 824
Fragments arranged by size:
824 233 191
StuI AGG'CCT
Cuts at: 0 856 1248
Size: 856 392
StyI C'CwwG_G
Cuts at: 0 879 1248
Size: 879 369
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 390 765 1026 1248
Size: 390 375 261 222
Fragments arranged by size:
390 375 261 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 755 787 1248
Size: 755 32 461
Fragments arranged by size:
755 461 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 859 1248
Size: 859 389
XhoI C'TCGA_G
Cuts at: 0 191 1248
Size: 191 1057
XmnI GAAnn'nnTTC
Cuts at: 0 796 1248
Size: 796 452
Enzymes that do cut:
AarI AccI AcuI AhdI AlwNI AvaI BanI BanII
BbsI BcgI BclI BglII BlpI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrFI BstXI BstYI BtgI
BtsI DraI DraIII EaeI EarI Eco57MI EcoO109I Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NspI PasI PfoI PpuMI PsiI PspXI PstI PvuII
SanDI SapI SfcI SmlI StuI StyI TaqII TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BfrBI BglI
Bme1580I BmgBI BmtI BsaAI BsaBI BsaXI BsgI BsiWI
BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PshAI PspOMI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XmaI
ZraI