Restriction map for
pFN21AE1407
(Circular) (Six-base) MAPSORT of: pFN21AE1407.seq Check: 9860 from: 1 to: 5680
pFN21AE1407 5680 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2140 2140
Size: 5680
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 2065 3001 2065
Size: 936 4744
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3743 5127 5395 1632
Size: 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 2800 3423 4995 2800
Size: 623 1572 3485
Fragments arranged by size:
3485 1572 623
ApaI G_GGCC'C
Cuts at: 2973 2973
Size: 5680
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2092 2279 2782 2989 3215 3367 5476 1493
Size: 599 187 503 207 226 152 2109 1697
Fragments arranged by size:
2109 1697 599 503 226 207 187 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2948 3005 1870
Size: 73 1005 57 4545
Fragments arranged by size:
4545 1005 73 57
AvrII C'CTAG_G
Cuts at: 2777 2777
Size: 5680
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 3001 4456 618
Size: 325 217 627 278 936 1455 1842
Fragments arranged by size:
1842 1455 936 627 325 278 217
BanII G_rGCy'C
Cuts at: 1391 2973 2999 1391
Size: 1582 26 4072
Fragments arranged by size:
4072 1582 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2689 2762 2910 928
Size: 971 790 73 148 3698
Fragments arranged by size:
3698 971 790 148 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BfrBI ATG'CAT
Cuts at: 2202 2202
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2973 3741 4897 1790
Size: 1183 768 1156 2573
Fragments arranged by size:
2573 1183 1156 768
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2311 2512 3411
Size: 416 53 83 399 24 894 201 899
Cuts at: 3411 4369 442
Size: 958 1753
Fragments arranged by size:
1753 958 899 894 416 399 201 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2567 4340 1239
Size: 1328 1773 2579
Fragments arranged by size:
2579 1773 1328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2218 2284 3805 4670 4968 2218
Size: 66 1521 865 298 2930
Fragments arranged by size:
2930 1521 865 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2756 2786 1178
Size: 30 165 30 1353 30 4072
Fragments arranged by size:
4072 1353 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2708 1186
Size: 1522 4158
BseYI C'CCAG_C
Cuts at: 2029 2606 4883 5379 2029
Size: 577 2277 496 2330
Fragments arranged by size:
2330 2277 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3741 3826 4897 3741
Size: 85 1071 4524
Fragments arranged by size:
4524 1071 85
BsmI GAATG_Cn'
Cuts at: 2293 2361 3191 3284 2293
Size: 68 830 93 4689
Fragments arranged by size:
4689 830 93 68
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2973 2999 3741 3826 4897 1391
Size: 399 1183 26 742 85 1071 2174
Fragments arranged by size:
2174 1183 1071 742 399 85 26
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 3035 844
Size: 1296 895 3489
Fragments arranged by size:
3489 1296 895
BsrBI CCG'CTC
Cuts at: 1394 1823 2916 3567 1394
Size: 429 1093 651 3507
Fragments arranged by size:
3507 1093 651 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2895 4175 4349 65
Size: 1183 1647 1280 174 1396
Fragments arranged by size:
1647 1396 1280 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 2252 1184
Size: 1068 4612
BstYI r'GATC_y
Cuts at: 1972 3010 3377 3762 3779 5231 5317 5329 5675
Size: 1038 367 385 17 1452 86 12 346
Cuts at: 5675 1972
Size: 1977
Fragments arranged by size:
1977 1452 1038 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2552 513
Size: 552 939 548 3641
Fragments arranged by size:
3641 939 552 548
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4941
Fragments arranged by size:
4941 683 56
BtsI GCAGTG_nn'
Cuts at: 3196 3990 4010 3196
Size: 794 20 4866
Fragments arranged by size:
4866 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2531 2654 4687 809
Size: 507 1215 123 2033 1802
Fragments arranged by size:
2033 1802 1215 507 123
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2673 3127 4016
Size: 54 1425 50 275 18 843 454 889
Cuts at: 4016 5645 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 843 454 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2793 3610 1353
Size: 1440 817 3423
Fragments arranged by size:
3423 1440 817
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2567 3743 4340 5127 5395 1239
Size: 393 935 1176 597 787 268 1524
Fragments arranged by size:
1524 1176 935 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2533 2952 2969 2970 2533
Size: 419 17 1 5243
Fragments arranged by size:
5243 419 17 1
EcoRI G'AATT_C
Cuts at: 2092 2782 2989 3367 2092
Size: 690 207 378 4405
Fragments arranged by size:
4405 690 378 207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 5680
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 4827 1678
Size: 3149 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4321 4353 4395 4427 1373
Size: 32 135 32 2749 32 42 32 2626
Fragments arranged by size:
2749 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 2069 3005 2069
Size: 936 4744
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2841 4794 4978 1305
Size: 1536 1953 184 2007
Fragments arranged by size:
2007 1953 1536 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3858
Fragments arranged by size:
3858 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 3638 3997 4156 518
Size: 648 1518 954 359 159 2042
Fragments arranged by size:
2042 1518 954 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2940 3096 3773 4921 5166 1606
Size: 1334 156 677 1148 245 2120
Fragments arranged by size:
2120 1334 1148 677 245 156
NcoI C'CATG_G
Cuts at: 513 1065 2004 2552 513
Size: 552 939 548 3641
Fragments arranged by size:
3641 939 552 548
NdeI CA'TA_TG
Cuts at: 387 2388 387
Size: 2001 3679
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NsiI A_TGCA'T
Cuts at: 2204 2204
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 3038 4583 1132
Size: 97 1809 1545 2229
Fragments arranged by size:
2229 1809 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4778
PfoI T'CCnGG_A
Cuts at: 2663 2663
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2533 2952 2533
Size: 419 5261
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 2466 3250 2466
Size: 784 4896
PspOMI G'GGCC_C
Cuts at: 2969 2969
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2945 3032 830
Size: 2115 87 3478
Fragments arranged by size:
3478 2115 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2940 2940
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SanDI GG'GwC_CC
Cuts at: 2952 2952
Size: 5680
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 2370 3928 5640 2370
Size: 1558 1712 2410
Fragments arranged by size:
2410 1712 1558
SfcI C'TryA_G
Cuts at: 826 1045 2117 2941 3028 4163 4844 5035 826
Size: 219 1072 824 87 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 824 681 219 191 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2233 2263 3784 4685 4947 820
Size: 197 926 290 30 1521 901 262 1553
Fragments arranged by size:
1553 1521 926 901 290 262 197 30
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 2017 2552 2777 3119 513
Size: 552 939 13 535 225 342 3074
Fragments arranged by size:
3074 939 552 535 342 225 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2021 2683 3707 3724 3877 4062 4507
Size: 1255 441 662 1024 17 153 185 445
Cuts at: 4507 325
Size: 1498
Fragments arranged by size:
1498 1255 1024 662 445 441 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2368 3926 5638
Size: 274 80 33 51 1443 391 1558 1712
Cuts at: 5638 96
Size: 138
Fragments arranged by size:
1712 1558 1443 391 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2305 2337 5640 5672 2305
Size: 32 3303 32 2313
Fragments arranged by size:
3303 2313 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 2092 2502 3809 2092
Size: 410 1307 3963
Fragments arranged by size:
3963 1307 410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI