################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AlwI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI Hin1I HincII HindII HinfI HpaI KasI KpnI Ksp632I MaeI MaeII MaeIII MamI MboI MflI MluI MluNI MroI MscI MseI MslI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspI SwaI TfiI Tru9I Tsp45I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 12 CGCG CG^CG AcsI 69 GAATTT [GA]^AATT[CT] AflIII 60 ACATGT A^C[GA][CT]GT Alw44I 118 GTGCAC G^TGCAC AlwNI 227 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 23 GGGCCC GGGCC^C ApaLI 118 GTGCAC G^TGCAC ApoI 69 GAATTT [GA]^AATT[CT] AvaI 26 CCCGGG C^[CT]CG[GA]G AvaII 718 GGACC G^G[AT]CC BbsI 56 GAAGAC GAAGAC(2/6) BglI 25 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 56 GAAGAC GAAGAC(2/6) BseRI 104 GAGGAG GAGGAG(10/8) BsgI 402 CTGCAC GTGCAG(16/14) BsmAI 484 GAGAC GTCTC(1/5) BsmFI 717 GGGAC GGGAC(10/14) BsoBI 26 CCCGGG C^[CT]CG[GA]G Bsp1407I 732 TGTACA T^GTACA BspLU11I 60 ACATGT A^CATGT BsrGI 732 TGTACA T^GTACA BstBI 425 TTCGAA TT^CGAA BstUI 12 CGCG CG^CG CfoI 16 GCGC GCG^C DsaI 11 CCGCGG C^C[GA][CT]GG HaeII 15 GGCGCT [GA]GCGC^[CT] HgaI 662 GACGC GACGC(5/10) HhaI 16 GCGC GCG^C HinP1I 16 GCGC G^CGC HindIII 463 AAGCTT A^AGCTT HphI 41 TCACC GGTGA(8/7) KspI 11 CCGCGG CCGC^GG MfeI 194 CAATTG C^AATTG MunI 194 CAATTG C^AATTG MvnI 12 CGCG CG^CG NspV 425 TTCGAA TT^CGAA PflMI 652 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 717 GGGACCT [GA]G^G[AT]CC[CT] SacI 619 GAGCTC GAGCT^C SacII 11 CCGCGG CCGC^GG SfuI 425 TTCGAA TT^CGAA SmaI 26 CCCGGG CCC^GGG SmlI 622 CTCAAG C^T[CT][GA]AG SphI 419 GCATGC GCATG^C SspBI 732 TGTACA T^GTACA StuI 741 AGGCCT AGG^CCT TspRI 49 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 652 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 258 CCAAAGGCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 26 CCCGGG C^CCGGG XmaI 26 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 319 GTAC GT^AC 733 GTAC AspHI 118 GTGCAC G[AT]GC[AT]^C 619 GAGCTC BanII 23 GGGCCC G[GA]GC[CT]^C 619 GAGCTC BbvI 226 GCAGC GCAGC(8/12) 229 GCAGC BsiHKAI 118 GTGCAC G[AT]GC[AT]^C 619 GAGCTC DdeI 134 CTGAG C^T[ACGT]AG 593 CTAAG DraII 532 GGGGCCT [GA]G^G[ACGT]CC[CT] 717 GGGACCT EcoO109I 532 GGGGCCT [GA]G^G[ACGT]CC[CT] 717 GGGACCT EcoT14I 652 CCAAGG C^C[AT][AT]GG 713 CCAAGG HapII 21 CCGG C^CGG 27 CCGG HpaII 21 CCGG C^CGG 27 CCGG MspI 21 CCGG C^CGG 27 CCGG NlaIII 61 CATG CATG^ 420 CATG NspI 60 ACATGT [GA]CATG^[CT] 419 GCATGC PvuII 230 CAGCTG CAG^CTG 680 CAGCTG RsaI 319 GTAC GT^AC 733 GTAC StyI 652 CCAAGG C^C[AT][AT]GG 713 CCAAGG TaqI 369 TCGA T^CGA 426 TCGA TseI 226 GCAGC G^C[AT]GC 229 GCAGC TthHB8I 369 TCGA T^CGA 426 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AluI BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII ItaI MboII MnlI MspA1I MvaI MwoI NciI NlaIV Sau96I ScrFI Tsp509I