Restriction map for pFN21AE1378
 (Circular) (Six-base) MAPSORT of: pFN21AE1378.seq  Check: 9730  from: 1  to: 5452



pFN21AE1378  5452 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:46  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3262     278 
   Size:         53      83     186     936    1726    2468

  Fragments arranged by size: 

                2468    1726     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2795    1937 
   Size:        858    4594
 
Acc65I G'GTAC_C

Cuts at:   2773    2773 
   Size:       5452
 
AclI AA'CG_TT

Cuts at:   1563    3579    3952    1563 
   Size:       2016     373    3063

  Fragments arranged by size: 

                3063    2016     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3515    4899    5167    1632 
   Size:       1883    1384     268    1917

  Fragments arranged by size: 

                1917    1883    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5255
 
AflIII A'CryG_T

Cuts at:   1203    2065    4351    5328    1203 
   Size:        862    2286     977    1327

  Fragments arranged by size: 

                2286    1327     977     862
 
AgeI A'CCGG_T

Cuts at:   4283    4283 
   Size:       5452
 
AhdI GACnn_n'nnGTC

Cuts at:   4181    4181 
   Size:       5452
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5452
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5452
 
AlwNI CAG_nnn'CTG

Cuts at:   2237    3195    4767    2237 
   Size:        958    1572    2922

  Fragments arranged by size: 

                2922    1572     958
 
ApaI G_GGCC'C

Cuts at:   2032    2032 
   Size:       5452
 
ApaLI G'TGCA_C

Cuts at:   2123    3509    4665    2123 
   Size:       1386    1156    2910

  Fragments arranged by size: 

                2910    1386    1156
 
ApoI r'AATT_y

Cuts at:   1493    2074    2761    2987    3139    5248    1493 
   Size:        581     687     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     687     581     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4006     160 
   Size:        872    2974    1606

  Fragments arranged by size: 

                2974    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5452
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2031    2777    1870 
   Size:         73      88     746    4545

  Fragments arranged by size: 

                4545     746      88      73
 
BamHI G'GATC_C

Cuts at:   2782    3149    2782 
   Size:        367    5085
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2773    4228     618 
   Size:        325     217     627     986    1455    1842

  Fragments arranged by size: 

                1842    1455     986     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2032    2628    2771    1391 
   Size:        641     596     143    4072

  Fragments arranged by size: 

                4072     641     596     143
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2068     928 
   Size:        971     169    4312

  Fragments arranged by size: 

                4312     971     169
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3643    3677    1903 
   Size:         34    1706      34    3678

  Fragments arranged by size: 

                3678    1706      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3344    4554    3344 
   Size:       1210    4242
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5452
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2036    4063     136 
   Size:        107     122      71     631     605     364    2027    1525

  Fragments arranged by size: 

                2027    1525     631     605     364     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5447    5447 
   Size:       5452
 
BlpI GC'TnA_GC

Cuts at:   2869    2869 
   Size:       5452
 
Bme1580I G_kGCm'C

Cuts at:   1790    2032    2127    3513    4669    1790 
   Size:        242      95    1386    1156    2573

  Fragments arranged by size: 

                2573    1386    1156     242      95
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5452
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2065    3183    4141 
   Size:        416      53      83     399      24     648    1118     958

Cuts at:   4141     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1118     958     648     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5452
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2127    2471    2709    4112    1239 
   Size:        888     344     238    1403    2579

  Fragments arranged by size: 

                2579    1403     888     344     238
 
Bpu10I CC'TnA_GC

Cuts at:   2598    2598 
   Size:       5452
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2612    3577    4442    4740    2612 
   Size:        965     865     298    3324

  Fragments arranged by size: 

                3324     965     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4115     882 
   Size:        617    2616    2219

  Fragments arranged by size: 

                2616    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3243     493 
   Size:        711    2039    2702

  Fragments arranged by size: 

                2702    2039     711
 
BsaBI GATnn'nnATC

Cuts at:   5446    5446 
   Size:       5452
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3259    3641    4276     275 
   Size:         53      83     186     936    1726     382     635    1451

  Fragments arranged by size: 

                1726    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3885    4283    4557    4704    5115    3885 
   Size:        398     274     147     411    4222

  Fragments arranged by size: 

                4222     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5227

  Fragments arranged by size: 

                5227     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2124    1186 
   Size:        938    4514
 
BseYI C'CCAG_C

Cuts at:   4655    5151    4655 
   Size:        496    4956
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2392    1163 
   Size:        573     656    4223

  Fragments arranged by size: 

                4223     656     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2902    3663    4691    1540 
   Size:        275     186     901     761    1028    2301

  Fragments arranged by size: 

                2301    1028     901     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2127    2628    3513    3598    4669    2127 
   Size:        501     885      85    1071    2910

  Fragments arranged by size: 

                2910    1071     885     501      85
 
BsmI GAATG_Cn'

Cuts at:   2963    3056    2963 
   Size:         93    5359
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2032    2127    2628    2771    3513    3598    4669 
   Size:        399     242      95     501     143     742      85    1071

Cuts at:   4669    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     501     399     242     143      95
                  85
 
BspEI T'CCGG_A

Cuts at:   5115    5115 
   Size:       5452
 
BspHI T'CATG_A

Cuts at:   3341    5071    3341 
   Size:       1730    3722
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2807     844 
   Size:       1963    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3339    1394 
   Size:        429    1516    3507

  Fragments arranged by size: 

                3507    1516     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3947    4121      65 
   Size:       1183    2699     174    1396

  Fragments arranged by size: 

                2699    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4096    4283    1700 
   Size:        110    2286     187    2869

  Fragments arranged by size: 

                2869    2286     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2737      96 
   Size:       2641    2811
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5452
 
BssSI C'ACGA_G

Cuts at:   3512    4524    3512 
   Size:       1012    4440
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5452
 
BstBI TT'CG_AA

Cuts at:   2431    4256    2431 
   Size:       1825    3627
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5452
 
BstYI r'GATC_y

Cuts at:   1972    2782    3149    3534    3551    5003    5089    5101    5447 
   Size:        810     367     385      17    1452      86      12     346

Cuts at:   5447    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     810     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2016     513 
   Size:        552     951    3949

  Fragments arranged by size: 

                3949     951     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4713

  Fragments arranged by size: 

                4713     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2968    3762    3782    2968 
   Size:        794      20    4638

  Fragments arranged by size: 

                4638     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5452
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5417
 
DraI TTT'AAA

Cuts at:   2756    2756 
   Size:       5452
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4459     809 
   Size:        507    3143    1802

  Fragments arranged by size: 

                3143    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2899    3788    5417 
   Size:         54    1425      50     275      18    1069     889    1629

Cuts at:   5417       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1069     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2899    1537 
   Size:        275    1087    4090

  Fragments arranged by size: 

                4090    1087     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3382    1353 
   Size:       2029    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4039    4413    4559     387 
   Size:       1064     138    2450     374     146    1280

  Fragments arranged by size: 

                2450    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2626    2626 
   Size:       5452
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2127    2471    2709    3515    4112    4899    5167 
   Size:        393     495     344     238     806     597     787     268

Cuts at:   5167    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     806     787     597     495     393     344     268
                 238
 
EcoO109I rG'GnC_Cy

Cuts at:   2538    2723    2538 
   Size:        185    5267
 
EcoRI G'AATT_C

Cuts at:   2761    3139    2761 
   Size:        378    5074
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2765    2765 
   Size:       5452
 
FspI TGC'GCA

Cuts at:   3958    3958 
   Size:       5452
 
HaeII r_GCGC'y

Cuts at:   1678    2024    4599    1678 
   Size:        346    2575    2531

  Fragments arranged by size: 

                2575    2531     346
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4093    4125    4167    4199    1373 
   Size:         32     135      32    2521      32      42      32    2626

  Fragments arranged by size: 

                2626    2521     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2796    3042     669 
   Size:        988     281     858     246    3079

  Fragments arranged by size: 

                3079     988     858     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2468     748 
   Size:       1720    3732
 
HpaI GTT'AAC

Cuts at:   3042    3042 
   Size:       5452
 
KpnI G_GTAC'C

Cuts at:   2777    2777 
   Size:       5452
 
MfeI C'AATT_G

Cuts at:   2199    3051    2199 
   Size:        852    4600
 
MluI A'CGCG_T

Cuts at:   5328    5328 
   Size:       5452
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2365    4566    4750    1305 
   Size:       1060    2201     184    2007

  Fragments arranged by size: 

                2201    2007    1060     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3630

  Fragments arranged by size: 

                3630    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3410    3769    3928     518 
   Size:        648    2244     359     159    2042

  Fragments arranged by size: 

                2244    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2018    2237    2687    2868    3545    4693    4938    1606 
   Size:        412     219     450     181     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     450     412     245     219     181
            
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4900
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5452
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5452
 
NotI GC'GGCC_GC

Cuts at:   2899    2899 
   Size:       5452
 
NspI r_CATG'y

Cuts at:   1132    1229    2069    2428    2810    4355    1132 
   Size:         97     840     359     382    1545    2229

  Fragments arranged by size: 

                2229    1545     840     382     359      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5452
 
PciI A'CATG_T

Cuts at:   2065    4351    2065 
   Size:       2286    3166
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2663    1108 
   Size:       1555    3897
 
PfoI T'CCnGG_A

Cuts at:   2250    2250 
   Size:       5452
 
PmeI GTTT'AAAC

Cuts at:   2756    2756 
   Size:       5452
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3521    3553    5034    5066    3521 
   Size:         32    1481      32    3907

  Fragments arranged by size: 

                3907    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2723    2723 
   Size:       5452
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5452
 
PsiI TTA'TAA

Cuts at:   3022    3022 
   Size:       5452
 
PspOMI G'GGCC_C

Cuts at:   2028    2028 
   Size:       5452
 
PstI C_TGCA'G

Cuts at:    830    2804     830 
   Size:       1974    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5452
 
PvuII CAG'CTG

Cuts at:   2237    2687    2237 
   Size:        450    5002
 
SacI G_AGCT'C

Cuts at:   2628    2628 
   Size:       5452
 
SacII CC_GC'GG

Cuts at:   2019    2019 
   Size:       5452
 
SalI G'TCGA_C

Cuts at:   1936    2794    1936 
   Size:        858    4594
 
SbfI CC_TGCA'GG

Cuts at:   2804    2804 
   Size:       5452
 
ScaI AGT'ACT

Cuts at:   3700    5412    3700 
   Size:       1712    3740
 
SfcI C'TryA_G

Cuts at:    826    1045    2800    3935    4616    4807     826 
   Size:        219    1755    1135     681     191    1471

  Fragments arranged by size: 

                1755    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5452
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5452
 
SmaI CCC'GGG

Cuts at:   1872    2033    2779    1872 
   Size:        161     746    4545

  Fragments arranged by size: 

                4545     746     161
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2627    3556    4457    4719     820 
   Size:        197     926     684     929     901     262    1553

  Fragments arranged by size: 

                1553     929     926     901     684     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3243     493 
   Size:       2750    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5452
 
SphI G_CATG'C

Cuts at:   1132    2428    2810    1132 
   Size:       1296     382    3774

  Fragments arranged by size: 

                3774    1296     382
 
SrfI GCCC'GGGC

Cuts at:   2033    2033 
   Size:       5452
 
SspI AAT'ATT

Cuts at:      5      52    3376       5 
   Size:         47    3324    2081

  Fragments arranged by size: 

                3324    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2748    1517 
   Size:       1231    4221
 
StyI C'CwwG_G

Cuts at:    513    1065    2657    2718    2891     513 
   Size:        552    1592      61     173    3074

  Fragments arranged by size: 

                3074    1592     552     173      61
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2265    2731    3479    3496    3649    3834    4279 
   Size:       1255     685     466     748      17     153     185     445

Cuts at:   4279     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255     748     685     466     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2737    3698    5410 
   Size:        274      80      33      51    1443     760     961    1712

Cuts at:   5410      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443     961     760     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5412    5444    5412 
   Size:         32    5420
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5452
 
XbaI T'CTAG_A

Cuts at:   2788    2788 
   Size:       5452
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2270    2270 
   Size:       5452
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5452
 
XmaI C'CCGG_G

Cuts at:   1870    2031    2777    1870 
   Size:        161     746    4545

  Fragments arranged by size: 

                4545     746     161
 
XmnI GAAnn'nnTTC

Cuts at:   3581    3581 
   Size:       5452
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3260     276 
   Size:         53      83     186     936    1726    2468

  Fragments arranged by size: 

                2468    1726     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PstI     PvuI    PvuII     SacI    SacII     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI    PspXI     PsrI    RsrII    SanDI
     SapI    SexAI     SfiI     SfoI     SwaI
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