(Circular) (Six-base) MAPSORT of: pFN21AB5749.seq Check: 5779 from: 1 to: 7129 pFN21AB5749 7129 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2661 4939 278 Size: 53 83 186 936 1125 2278 2468 Fragments arranged by size: 2468 2278 1125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2580 3369 4472 1937 Size: 643 789 1103 4594 Fragments arranged by size: 4594 1103 789 643 Acc65I G'GTAC_C Cuts at: 2012 4450 2012 Size: 2438 4691 AclI AA'CG_TT Cuts at: 1563 5256 5629 1563 Size: 3693 373 3063 Fragments arranged by size: 3693 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2755 4117 4332 5192 6576 6844 1632 Size: 1123 1362 215 860 1384 268 1917 Fragments arranged by size: 1917 1384 1362 1123 860 268 215 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6932 AflIII A'CryG_T Cuts at: 1203 6028 7005 1203 Size: 4825 977 1327 Fragments arranged by size: 4825 1327 977 AgeI A'CCGG_T Cuts at: 5960 5960 Size: 7129 AhdI GACnn_n'nnGTC Cuts at: 3244 5858 3244 Size: 2614 4515 AleI CACnn'nnGTG Cuts at: 1166 2498 3009 1166 Size: 1332 511 5286 Fragments arranged by size: 5286 1332 511 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2974 1184 Size: 1790 5339 AlwNI CAG_nnn'CTG Cuts at: 3067 4872 6444 3067 Size: 1805 1572 3752 Fragments arranged by size: 3752 1805 1572 ApaLI G'TGCA_C Cuts at: 5186 6342 5186 Size: 1156 5973 ApoI r'AATT_y Cuts at: 1493 2094 3233 4438 4664 4816 6925 1493 Size: 601 1139 1205 226 152 2109 1697 Fragments arranged by size: 2109 1697 1205 1139 601 226 152 AseI AT'TA_AT Cuts at: 160 1032 5683 160 Size: 872 4651 1606 Fragments arranged by size: 4651 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 AvaI C'yCGr_G Cuts at: 1870 1943 3093 4454 1870 Size: 73 1150 1361 4545 Fragments arranged by size: 4545 1361 1150 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2004 2037 2004 Size: 33 7096 BamHI G'GATC_C Cuts at: 3472 4459 4826 3472 Size: 987 367 5775 Fragments arranged by size: 5775 987 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 4450 5905 618 Size: 325 217 627 225 2438 1455 1842 Fragments arranged by size: 2438 1842 1455 627 325 225 217 BanII G_rGCy'C Cuts at: 1391 3122 3818 4341 4448 1391 Size: 1731 696 523 107 4072 Fragments arranged by size: 4072 1731 696 523 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 2468 4088 928 Size: 971 192 377 1620 3969 Fragments arranged by size: 3969 1620 971 377 192 BbvCI CC'TCA_GC Cuts at: 2366 2838 2366 Size: 472 6657 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5320 5354 1903 Size: 34 3383 34 3678 Fragments arranged by size: 3678 3383 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2933 5021 6231 2933 Size: 2088 1210 3831 Fragments arranged by size: 3831 2088 1210 BclI T'GATC_A Cuts at: 1548 2121 1548 Size: 573 6556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5740 136 Size: 107 122 71 631 605 4068 1525 Fragments arranged by size: 4068 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3492 7124 3492 Size: 3632 3497 BlpI GC'TnA_GC Cuts at: 2820 4546 2820 Size: 1726 5403 Bme1580I G_kGCm'C Cuts at: 1790 5190 6346 1790 Size: 3400 1156 2573 Fragments arranged by size: 3400 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2335 1318 Size: 1017 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4860 5818 442 Size: 416 53 83 399 24 3443 958 1753 Fragments arranged by size: 3443 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7129 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4360 4360 Size: 7129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2497 3147 3822 3998 5789 1239 Size: 1258 650 675 176 1791 2579 Fragments arranged by size: 2579 1791 1258 675 650 176 Bpu10I CC'TnA_GC Cuts at: 2366 2838 3477 2366 Size: 472 639 6018 Fragments arranged by size: 6018 639 472 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2576 4143 5254 6119 6417 2576 Size: 1567 1111 865 298 3288 Fragments arranged by size: 3288 1567 1111 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2979 3033 4187 5792 882 Size: 617 1480 54 1154 1605 2219 Fragments arranged by size: 2219 1605 1480 1154 617 54 BsaAI yAC'GTr Cuts at: 493 1204 2302 4920 493 Size: 711 1098 2618 2702 Fragments arranged by size: 2702 2618 1098 711 BsaBI GATnn'nnATC Cuts at: 4389 7123 4389 Size: 2734 4395 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2658 4936 5318 5953 Size: 53 83 186 936 1125 2278 382 635 Cuts at: 5953 275 Size: 1451 Fragments arranged by size: 2278 1451 1125 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5562 5960 6234 6381 6792 5562 Size: 398 274 147 411 5899 Fragments arranged by size: 5899 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6904 Fragments arranged by size: 6904 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2306 2555 3112 4052 4160 1186 Size: 1045 75 249 557 940 108 4155 Fragments arranged by size: 4155 1045 940 557 249 108 75 BseYI C'CCAG_C Cuts at: 2629 6332 6828 2629 Size: 3703 496 2930 Fragments arranged by size: 3703 2930 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3072 3717 1163 Size: 573 1336 645 4575 Fragments arranged by size: 4575 1336 645 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4579 5340 6368 1540 Size: 275 186 2578 761 1028 2301 Fragments arranged by size: 2578 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3532 3818 5190 5275 6346 3532 Size: 286 1372 85 1071 4315 Fragments arranged by size: 4315 1372 1071 286 85 BsmI GAATG_Cn' Cuts at: 3337 3701 4241 4640 4733 3337 Size: 364 540 399 93 5733 Fragments arranged by size: 5733 540 399 364 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2341 2341 Size: 7129 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3122 3532 3818 4341 4448 5190 5275 Size: 399 1332 410 286 523 107 742 85 Cuts at: 5275 6346 1391 Size: 1071 2174 Fragments arranged by size: 2174 1332 1071 742 523 410 399 286 107 85 BspEI T'CCGG_A Cuts at: 6792 6792 Size: 7129 BspHI T'CATG_A Cuts at: 3705 5018 6748 3705 Size: 1313 1730 4086 Fragments arranged by size: 4086 1730 1313 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 3366 3927 4484 844 Size: 1328 1194 561 557 3489 Fragments arranged by size: 3489 1328 1194 561 557 BsrBI CCG'CTC Cuts at: 1394 1823 2649 5016 1394 Size: 429 826 2367 3507 Fragments arranged by size: 3507 2367 826 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3300 3771 5624 5798 65 Size: 1183 2052 471 1853 174 1396 Fragments arranged by size: 2052 1853 1396 1183 471 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2139 2193 2633 5773 5960 1700 Size: 110 329 54 440 3140 187 2869 Fragments arranged by size: 3140 2869 440 329 187 110 54 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7129 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7129 BssSI C'ACGA_G Cuts at: 5189 6201 5189 Size: 1012 6117 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7129 BstBI TT'CG_AA Cuts at: 5933 5933 Size: 7129 BstEII G'GTnAC_C Cuts at: 1184 2699 1184 Size: 1515 5614 BstXI CCAn_nnnn'nTGG Cuts at: 2500 2911 3011 3202 2500 Size: 411 100 191 6427 Fragments arranged by size: 6427 411 191 100 BstYI r'GATC_y Cuts at: 1972 3472 3492 4356 4459 4826 5211 5228 6680 Size: 1500 20 864 103 367 385 17 1452 Cuts at: 6680 6766 6778 7124 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1500 1452 864 385 367 346 103 86 20 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2342 2926 3010 3195 513 Size: 552 939 12 326 584 84 185 4447 Fragments arranged by size: 4447 939 584 552 326 185 84 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3564 487 Size: 683 56 2338 4052 Fragments arranged by size: 4052 2338 683 56 BtsI GCAGTG_nn' Cuts at: 2440 3084 3349 3425 4645 5439 5459 2440 Size: 644 265 76 1220 794 20 4110 Fragments arranged by size: 4110 1220 794 644 265 76 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7129 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3754 3789 529 Size: 35 3190 35 3869 Fragments arranged by size: 3869 3190 35 35 DraI TTT'AAA Cuts at: 4433 4433 Size: 7129 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3757 4394 6136 809 Size: 507 2441 637 1742 1802 Fragments arranged by size: 2441 1802 1742 637 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2635 2901 3112 Size: 54 1425 50 275 18 805 266 211 Cuts at: 3112 3585 3726 3762 3876 4576 5465 7094 8 Size: 473 141 36 114 700 889 1629 43 Fragments arranged by size: 1629 1425 889 805 700 473 275 266 211 141 114 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 4576 1537 Size: 275 2764 4090 Fragments arranged by size: 4090 2764 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 3455 3774 4029 4061 4067 4329 4351 Size: 824 1278 319 255 32 6 262 22 Cuts at: 4351 5059 1353 Size: 708 3423 Fragments arranged by size: 3423 1278 824 708 319 262 255 32 22 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5716 6090 6236 387 Size: 1064 138 4127 374 146 1280 Fragments arranged by size: 4127 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3816 3816 Size: 7129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2497 2755 3147 3822 3998 4117 4332 Size: 393 865 258 392 675 176 119 215 Cuts at: 4332 5192 5789 6576 6844 1239 Size: 860 597 787 268 1524 Fragments arranged by size: 1524 865 860 787 675 597 393 392 268 258 215 176 119 EcoNI CCTnn'n_nnAGG Cuts at: 3752 3752 Size: 7129 EcoO109I rG'GnC_Cy Cuts at: 2626 2766 2626 Size: 140 6989 EcoRI G'AATT_C Cuts at: 4438 4816 4438 Size: 378 6751 EcoRV GAT'ATC Cuts at: 2887 3580 2887 Size: 693 6436 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2905 2905 Size: 7129 FspI TGC'GCA Cuts at: 2168 3797 4284 5635 2168 Size: 1629 487 1351 3662 Fragments arranged by size: 3662 1629 1351 487 HaeII r_GCGC'y Cuts at: 1678 3178 6276 1678 Size: 1500 3098 2531 Fragments arranged by size: 3098 2531 1500 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3230 3262 4001 4033 5770 Size: 32 135 32 1658 32 739 32 1737 Cuts at: 5770 5802 5844 5876 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1737 1658 739 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4473 4719 669 Size: 988 281 2535 246 3079 Fragments arranged by size: 3079 2535 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7129 HpaI GTT'AAC Cuts at: 4719 4719 Size: 7129 KpnI G_GTAC'C Cuts at: 2016 4454 2016 Size: 2438 4691 MfeI C'AATT_G Cuts at: 4728 4728 Size: 7129 MluI A'CGCG_T Cuts at: 7005 7005 Size: 7129 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6243 6427 1305 Size: 4938 184 2007 Fragments arranged by size: 4938 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2903 3587 3728 3878 10 Size: 54 1425 343 1071 684 141 150 3261 Fragments arranged by size: 3261 1425 1071 684 343 150 141 54 MslI CAynn'nnrTG Cuts at: 518 1166 2498 2931 2937 3009 3722 5087 5446 Size: 648 1332 433 6 72 713 1365 359 Cuts at: 5446 5605 518 Size: 159 2042 Fragments arranged by size: 2042 1365 1332 713 648 433 359 159 72 6 MspA1I CmG'CkG Cuts at: 1606 2236 2344 2370 2899 3339 3356 4545 5222 Size: 630 108 26 529 440 17 1189 677 Cuts at: 5222 6370 6615 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1189 1148 677 630 529 440 245 108 26 17 NaeI GCC'GGC Cuts at: 2635 2635 Size: 7129 NcoI C'CATG_G Cuts at: 513 1065 2004 2926 3195 513 Size: 552 939 922 269 4447 Fragments arranged by size: 4447 939 922 552 269 NdeI CA'TA_TG Cuts at: 387 387 Size: 7129 NgoMIV G'CCGG_C Cuts at: 2633 2633 Size: 7129 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7129 NotI GC'GGCC_GC Cuts at: 4576 4576 Size: 7129 NspI r_CATG'y Cuts at: 1132 1229 4487 6032 1132 Size: 97 3258 1545 2229 Fragments arranged by size: 3258 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7129 PciI A'CATG_T Cuts at: 6028 6028 Size: 7129 PflMI CCAn_nnn'nTGG Cuts at: 1108 4213 1108 Size: 3105 4024 PfoI T'CCnGG_A Cuts at: 2070 2607 2737 4269 2070 Size: 537 130 1532 4930 Fragments arranged by size: 4930 1532 537 130 PmeI GTTT'AAAC Cuts at: 4433 4433 Size: 7129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5198 5230 6711 6743 5198 Size: 32 1481 32 5584 Fragments arranged by size: 5584 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2766 2766 Size: 7129 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7129 PsiI TTA'TAA Cuts at: 4699 4699 Size: 7129 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2279 2311 2279 Size: 32 7097 PstI C_TGCA'G Cuts at: 830 3344 3924 4481 830 Size: 2514 580 557 3478 Fragments arranged by size: 3478 2514 580 557 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7129 PvuII CAG'CTG Cuts at: 2370 2899 3356 2370 Size: 529 457 6143 Fragments arranged by size: 6143 529 457 SacI G_AGCT'C Cuts at: 3818 3818 Size: 7129 SacII CC_GC'GG Cuts at: 2345 2345 Size: 7129 SalI G'TCGA_C Cuts at: 1936 4471 1936 Size: 2535 4594 SapI GCTCTTCn'nnn_ Cuts at: 3455 4329 3455 Size: 874 6255 SbfI CC_TGCA'GG Cuts at: 3924 4481 3924 Size: 557 6572 ScaI AGT'ACT Cuts at: 5377 7089 5377 Size: 1712 5417 SfcI C'TryA_G Cuts at: 826 1045 3340 3416 3920 4477 5612 6293 6484 Size: 219 2295 76 504 557 1135 681 191 Cuts at: 6484 826 Size: 1471 Fragments arranged by size: 2295 1471 1135 681 557 504 219 191 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7129 SmaI CCC'GGG Cuts at: 1872 4456 1872 Size: 2584 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 4158 5233 6134 6396 820 Size: 197 926 612 1603 1075 901 262 1553 Fragments arranged by size: 1603 1553 1075 926 901 612 262 197 SnaBI TAC'GTA Cuts at: 493 2302 4920 493 Size: 1809 2618 2702 Fragments arranged by size: 2702 2618 1809 SpeI A'CTAG_T Cuts at: 152 152 Size: 7129 SphI G_CATG'C Cuts at: 1132 4487 1132 Size: 3355 3774 SspI AAT'ATT Cuts at: 5 52 3025 5053 5 Size: 47 2973 2028 2081 Fragments arranged by size: 2973 2081 2028 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7129 StyI C'CwwG_G Cuts at: 513 1065 2004 2532 2880 2926 3195 3306 3561 Size: 552 939 528 348 46 269 111 255 Cuts at: 3561 4197 4568 513 Size: 636 371 3074 Fragments arranged by size: 3074 939 636 552 528 371 348 269 255 111 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3225 3644 5156 5173 5326 5511 5956 Size: 1255 1645 419 1512 17 153 185 445 Cuts at: 5956 325 Size: 1498 Fragments arranged by size: 1645 1512 1498 1255 445 419 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3714 4162 5375 Size: 274 80 33 51 1443 1737 448 1213 Cuts at: 5375 7087 96 Size: 1712 138 Fragments arranged by size: 1737 1712 1443 1213 448 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3580 3612 7089 7121 3580 Size: 32 3477 32 3588 Fragments arranged by size: 3588 3477 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2939 3503 1316 Size: 1623 564 4942 Fragments arranged by size: 4942 1623 564 XbaI T'CTAG_A Cuts at: 4465 4465 Size: 7129 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7129 XmaI C'CCGG_G Cuts at: 1870 4454 1870 Size: 2584 4545 XmnI GAAnn'nnTTC Cuts at: 4241 5258 4241 Size: 1017 6112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2659 4937 276 Size: 53 83 186 936 1125 2278 2468 Fragments arranged by size: 2468 2278 1125 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI