Restriction map for pFN21AB5749
 (Linear) (Six-base) MAPSORT of: KIBB5749.seq  Check: 5695  from: 1  to: 2424



KIBB5749.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AatII G_ACGT'C

Cuts at:      0     656    2424 
   Size:        656    1768
 
AccI GT'mk_AC

Cuts at:      0     575    1364    2424 
   Size:        575     789    1060

  Fragments arranged by size: 

                1060     789     575
 
Acc65I G'GTAC_C

Cuts at:      0       7    2424 
   Size:          7    2417
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     750    2112    2327    2424 
   Size:        750    1362     215      97

  Fragments arranged by size: 

                1362     750     215      97
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1239    2424 
   Size:       1239    1185
 
AleI CACnn'nnGTG

Cuts at:      0     493    1004    2424 
   Size:        493     511    1420

  Fragments arranged by size: 

                1420     511     493
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     969    2424 
   Size:        969    1455
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1062    2424 
   Size:       1062    1362
 
ApoI r'AATT_y

Cuts at:      0      89    1228    2424 
   Size:         89    1139    1196

  Fragments arranged by size: 

                1196    1139      89
 
AvaI C'yCGr_G

Cuts at:      0    1088    2424 
   Size:       1088    1336
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      32    2424 
   Size:         32    2392
 
BamHI G'GATC_C

Cuts at:      0    1467    2424 
   Size:       1467     957
 
BanI G'GyrC_C

Cuts at:      0       7    2424 
   Size:          7    2417
 
BanII G_rGCy'C

Cuts at:      0    1117    1813    2336    2424 
   Size:       1117     696     523      88

  Fragments arranged by size: 

                1117     696     523      88
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      86     463    2083    2424 
   Size:         86     377    1620     341

  Fragments arranged by size: 

                1620     377     341      86
 
BbvCI CC'TCA_GC

Cuts at:      0     361     833    2424 
   Size:        361     472    1591

  Fragments arranged by size: 

                1591     472     361
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     928    2424 
   Size:        928    1496
 
BclI T'GATC_A

Cuts at:      0     116    2424 
   Size:        116    2308
 
BglII A'GATC_T

Cuts at:      0    1487    2424 
   Size:       1487     937
 
BlpI GC'TnA_GC

Cuts at:      0     815    2424 
   Size:        815    1609
 
BmgBI CAC'GTC

Cuts at:      0     330    2424 
   Size:        330    2094
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2355    2424 
   Size:       2355      69
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     492    1142    1817    1993    2424 
   Size:        492     650     675     176     431

  Fragments arranged by size: 

                 675     650     492     431     176
 
Bpu10I CC'TnA_GC

Cuts at:      0     361     833    1472    2424 
   Size:        361     472     639     952

  Fragments arranged by size: 

                 952     639     472     361
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    2138    2424 
   Size:        571    1567     286

  Fragments arranged by size: 

                1567     571     286
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     974    1028    2182    2424 
   Size:        974      54    1154     242

  Fragments arranged by size: 

                1154     974     242      54
 
BsaAI yAC'GTr

Cuts at:      0     297    2424 
   Size:        297    2127
 
BsaBI GATnn'nnATC

Cuts at:      0    2384    2424 
   Size:       2384      40
 
BsaHI Gr'CG_yC

Cuts at:      0     653    2424 
   Size:        653    1771
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     226     301     550    1107    2047    2155    2424 
   Size:        226      75     249     557     940     108     269

  Fragments arranged by size: 

                 940     557     269     249     226     108      75
 
BseYI C'CCAG_C

Cuts at:      0     624    2424 
   Size:        624    1800
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1067    1712    2424 
   Size:       1067     645     712

  Fragments arranged by size: 

                1067     712     645
 
BsiHKAI G_wGCw'C

Cuts at:      0    1527    1813    2424 
   Size:       1527     286     611

  Fragments arranged by size: 

                1527     611     286
 
BsmI GAATG_Cn'

Cuts at:      0    1332    1696    2236    2424 
   Size:       1332     364     540     188

  Fragments arranged by size: 

                1332     540     364     188
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     336    2424 
   Size:        336    2088
 
Bsp1286I G_dGCh'C

Cuts at:      0    1117    1527    1813    2336    2424 
   Size:       1117     410     286     523      88

  Fragments arranged by size: 

                1117     523     410     286      88
 
BspHI T'CATG_A

Cuts at:      0    1700    2424 
   Size:       1700     724
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     167    1361    1922    2424 
   Size:        167    1194     561     502

  Fragments arranged by size: 

                1194     561     502     167
 
BsrBI CCG'CTC

Cuts at:      0     644    2424 
   Size:        644    1780
 
BsrDI GCAATG_nn'

Cuts at:      0    1295    1766    2424 
   Size:       1295     471     658

  Fragments arranged by size: 

                1295     658     471
 
BsrFI r'CCGG_y

Cuts at:      0     134     188     628    2424 
   Size:        134      54     440    1796

  Fragments arranged by size: 

                1796     440     134      54
 
BstEII G'GTnAC_C

Cuts at:      0     694    2424 
   Size:        694    1730
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     495     906    1006    1197    2424 
   Size:        495     411     100     191    1227

  Fragments arranged by size: 

                1227     495     411     191     100
 
BstYI r'GATC_y

Cuts at:      0    1467    1487    2351    2424 
   Size:       1467      20     864      73

  Fragments arranged by size: 

                1467     864      73      20
 
BtgI C'CryG_G

Cuts at:      0      11     337     921    1005    1190    2424 
   Size:         11     326     584      84     185    1234

  Fragments arranged by size: 

                1234     584     326     185      84      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1559    2424 
   Size:       1559     865
 
BtsI GCAGTG_nn'

Cuts at:      0     435    1079    1344    1420    2424 
   Size:        435     644     265      76    1004

  Fragments arranged by size: 

                1004     644     435     265      76
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1749    1784    2424 
   Size:       1749      35     640

  Fragments arranged by size: 

                1749     640      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1752    2389    2424 
   Size:       1752     637      35

  Fragments arranged by size: 

                1752     637      35
 
EaeI y'GGCC_r

Cuts at:      0     630     896    1107    1580    1721    1757    1871    2424 
   Size:        630     266     211     473     141      36     114     553

  Fragments arranged by size: 

                 630     553     473     266     211     141     114      36
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     172    1450    1769    2024    2056    2062    2324    2346 
   Size:        172    1278     319     255      32       6     262      22

Cuts at:   2346    2424 
   Size:         78

  Fragments arranged by size: 

                1278     319     262     255     172      78      32      22
                   6
 
EcoICRI GAG'CTC

Cuts at:      0    1811    2424 
   Size:       1811     613
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     492     750    1142    1817    1993    2112    2327    2424 
   Size:        492     258     392     675     176     119     215      97

  Fragments arranged by size: 

                 675     492     392     258     215     176     119      97
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1747    2424 
   Size:       1747     677
 
EcoO109I rG'GnC_Cy

Cuts at:      0     621     761    2424 
   Size:        621     140    1663

  Fragments arranged by size: 

                1663     621     140
 
EcoRV GAT'ATC

Cuts at:      0     882    1575    2424 
   Size:        882     693     849

  Fragments arranged by size: 

                 882     849     693
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     900    2424 
   Size:        900    1524
 
FspI TGC'GCA

Cuts at:      0     163    1792    2279    2424 
   Size:        163    1629     487     145

  Fragments arranged by size: 

                1629     487     163     145
 
HaeII r_GCGC'y

Cuts at:      0    1173    2424 
   Size:       1173    1251
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1225    1257    1996    2028    2424 
   Size:       1225      32     739      32     396

  Fragments arranged by size: 

                1225     739     396      32      32
 
KpnI G_GTAC'C

Cuts at:      0      11    2424 
   Size:         11    2413
 
MscI TGG'CCA

Cuts at:      0     898    1582    1723    1873    2424 
   Size:        898     684     141     150     551

  Fragments arranged by size: 

                 898     684     551     150     141
 
MslI CAynn'nnrTG

Cuts at:      0     493     926     932    1004    1717    2424 
   Size:        493     433       6      72     713     707

  Fragments arranged by size: 

                 713     707     493     433      72       6
 
MspA1I CmG'CkG

Cuts at:      0     231     339     365     894    1334    1351    2424 
   Size:        231     108      26     529     440      17    1073

  Fragments arranged by size: 

                1073     529     440     231     108      26      17
 
NaeI GCC'GGC

Cuts at:      0     630    2424 
   Size:        630    1794
 
NcoI C'CATG_G

Cuts at:      0     921    1190    2424 
   Size:        921     269    1234

  Fragments arranged by size: 

                1234     921     269
 
NgoMIV G'CCGG_C

Cuts at:      0     628    2424 
   Size:        628    1796
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2208    2424 
   Size:       2208     216
 
PfoI T'CCnGG_A

Cuts at:      0      65     602     732    2264    2424 
   Size:         65     537     130    1532     160

  Fragments arranged by size: 

                1532     537     160     130      65
 
PpuMI rG'GwC_Cy

Cuts at:      0     761    2424 
   Size:        761    1663
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     274     306    2424 
   Size:        274      32    2118

  Fragments arranged by size: 

                2118     274      32
 
PstI C_TGCA'G

Cuts at:      0    1339    1919    2424 
   Size:       1339     580     505

  Fragments arranged by size: 

                1339     580     505
 
PvuII CAG'CTG

Cuts at:      0     365     894    1351    2424 
   Size:        365     529     457    1073

  Fragments arranged by size: 

                1073     529     457     365
 
SacI G_AGCT'C

Cuts at:      0    1813    2424 
   Size:       1813     611
 
SacII CC_GC'GG

Cuts at:      0     340    2424 
   Size:        340    2084
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1450    2324    2424 
   Size:       1450     874     100

  Fragments arranged by size: 

                1450     874     100
 
SbfI CC_TGCA'GG

Cuts at:      0    1919    2424 
   Size:       1919     505
 
SfcI C'TryA_G

Cuts at:      0    1335    1411    1915    2424 
   Size:       1335      76     504     509

  Fragments arranged by size: 

                1335     509     504      76
 
SmlI C'TyrA_G

Cuts at:      0     550    2153    2424 
   Size:        550    1603     271

  Fragments arranged by size: 

                1603     550     271
 
SnaBI TAC'GTA

Cuts at:      0     297    2424 
   Size:        297    2127
 
SspI AAT'ATT

Cuts at:      0    1020    2424 
   Size:       1020    1404
 
StyI C'CwwG_G

Cuts at:      0     527     875     921    1190    1301    1556    2192    2424 
   Size:        527     348      46     269     111     255     636     232

  Fragments arranged by size: 

                 636     527     348     269     255     232     111      46
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1220    1639    2424 
   Size:       1220     419     785

  Fragments arranged by size: 

                1220     785     419
 
TatI w'GTAC_w

Cuts at:      0    1709    2157    2424 
   Size:       1709     448     267

  Fragments arranged by size: 

                1709     448     267
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1575    1607    2424 
   Size:       1575      32     817

  Fragments arranged by size: 

                1575     817      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     934    1498    2424 
   Size:        934     564     926

  Fragments arranged by size: 

                 934     926     564
 
XmnI GAAnn'nnTTC

Cuts at:      0    2236    2424 
   Size:       2236     188
 
ZraI GAC'GTC

Cuts at:      0     654    2424 
   Size:        654    1770
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AhdI     AleI     AlfI    AlwNI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI
    BciVI     BclI    BglII     BlpI    BmgBI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FspI    HaeII    Hin4I     KpnI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV    PflMI     PfoI    PpuMI     PsrI     PstI
    PvuII     SacI    SacII     SapI     SbfI     SfcI     SmlI    SnaBI
     SspI     StyI    TaqII     TatI     TstI  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII   AflIII     AgeI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BbeI     BcgI    BfrBI
     BglI Bme1580I     BmrI     BmtI    BsaWI    BsaXI    BsiEI    BsiWI
    BspEI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I
     ClaI     DraI   DraIII     EagI     EciI    EcoRI     FseI    FspAI
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     NarI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PvuI    RsrII     SalI    SanDI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI     StuI     SwaI
     XbaI     XcmI     XhoI     XmaI
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