(Circular) (Six-base) MAPSORT of: pFN21AE1377.seq Check: 8121 from: 1 to: 6283 pFN21AE1377 6283 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4093 278 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3626 1937 Size: 1689 4594 Acc65I G'GTAC_C Cuts at: 3604 3604 Size: 6283 AclI AA'CG_TT Cuts at: 1563 4410 4783 1563 Size: 2847 373 3063 Fragments arranged by size: 3063 2847 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2074 2174 2187 2445 2698 3385 4346 5730 Size: 442 100 13 258 253 687 961 1384 Cuts at: 5730 5998 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 961 687 442 268 258 253 100 13 AflII C'TTAA_G Cuts at: 820 1017 3371 820 Size: 197 2354 3732 Fragments arranged by size: 3732 2354 197 AflIII A'CryG_T Cuts at: 1203 5182 6159 1203 Size: 3979 977 1327 Fragments arranged by size: 3979 1327 977 AgeI A'CCGG_T Cuts at: 5114 5114 Size: 6283 AhdI GACnn_n'nnGTC Cuts at: 3139 5012 3139 Size: 1873 4410 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6283 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6283 AlwNI CAG_nnn'CTG Cuts at: 2031 4026 5598 2031 Size: 1995 1572 2716 Fragments arranged by size: 2716 1995 1572 ApaLI G'TGCA_C Cuts at: 3071 4340 5496 3071 Size: 1269 1156 3858 Fragments arranged by size: 3858 1269 1156 ApoI r'AATT_y Cuts at: 1493 3592 3818 3970 6079 1493 Size: 2099 226 152 2109 1697 Fragments arranged by size: 2109 2099 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4837 160 Size: 872 3805 1606 Fragments arranged by size: 3805 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 AvaI C'yCGr_G Cuts at: 1870 1943 3107 3192 3608 1870 Size: 73 1164 85 416 4545 Fragments arranged by size: 4545 1164 416 85 73 AvrII C'CTAG_G Cuts at: 2599 2599 Size: 6283 BamHI G'GATC_C Cuts at: 2904 3613 3980 2904 Size: 709 367 5207 Fragments arranged by size: 5207 709 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3211 3604 5059 618 Size: 325 217 627 1424 393 1455 1842 Fragments arranged by size: 1842 1455 1424 627 393 325 217 BanII G_rGCy'C Cuts at: 1391 2080 2346 2466 2722 2781 3114 3159 3602 Size: 689 266 120 256 59 333 45 443 Cuts at: 3602 1391 Size: 4072 Fragments arranged by size: 4072 689 443 333 266 256 120 59 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2992 3104 3231 3546 928 Size: 971 1093 112 127 315 3665 Fragments arranged by size: 3665 1093 971 315 127 112 BbvCI CC'TCA_GC Cuts at: 2913 3366 2913 Size: 453 5830 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4474 4508 1903 Size: 34 2537 34 3678 Fragments arranged by size: 3678 2537 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4175 5385 4175 Size: 1210 5073 BclI T'GATC_A Cuts at: 1548 1548 Size: 6283 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4894 136 Size: 107 122 71 631 605 3222 1525 Fragments arranged by size: 3222 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2709 6278 2709 Size: 3569 2714 BlpI GC'TnA_GC Cuts at: 2577 2679 3179 3700 2577 Size: 102 500 521 5160 Fragments arranged by size: 5160 521 500 102 Bme1580I G_kGCm'C Cuts at: 1790 3075 3216 4344 5500 1790 Size: 1285 141 1128 1156 2573 Fragments arranged by size: 2573 1285 1156 1128 141 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6283 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2842 4014 4972 Size: 416 53 83 399 24 1425 1172 958 Cuts at: 4972 442 Size: 1753 Fragments arranged by size: 1753 1425 1172 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6283 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2258 2953 2258 Size: 695 5588 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2330 2765 3173 3452 4943 1239 Size: 1091 435 408 279 1491 2579 Fragments arranged by size: 2579 1491 1091 435 408 279 Bpu10I CC'TnA_GC Cuts at: 2913 3366 2913 Size: 453 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3050 4408 5273 5571 3050 Size: 1358 865 298 3762 Fragments arranged by size: 3762 1358 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4946 882 Size: 617 3447 2219 Fragments arranged by size: 3447 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4074 493 Size: 711 2870 2702 Fragments arranged by size: 2870 2702 711 BsaBI GATnn'nnATC Cuts at: 6277 6277 Size: 6283 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4090 4472 5107 275 Size: 53 83 186 936 2557 382 635 1451 Fragments arranged by size: 2557 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4716 5114 5388 5535 5946 4716 Size: 398 274 147 411 5053 Fragments arranged by size: 5053 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 3141 3171 1178 Size: 30 165 30 693 30 1015 30 4290 Fragments arranged by size: 4290 1015 693 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2952 1186 Size: 1766 4517 BseYI C'CCAG_C Cuts at: 2956 2991 5486 5982 2956 Size: 35 2495 496 3257 Fragments arranged by size: 3257 2495 496 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5710 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2616 3733 4494 5522 1540 Size: 275 186 615 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 615 275 186 BsiHKAI G_wGCw'C Cuts at: 2781 3075 3159 4344 4429 5500 2781 Size: 294 84 1185 85 1071 3564 Fragments arranged by size: 3564 1185 1071 294 85 84 BsmI GAATG_Cn' Cuts at: 3794 3887 3794 Size: 93 6190 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2346 2466 2722 2781 3075 3114 Size: 399 290 266 120 256 59 294 39 Cuts at: 3114 3159 3216 3602 4344 4429 5500 1391 Size: 45 57 386 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 386 294 290 266 256 120 85 59 57 45 39 BspEI T'CCGG_A Cuts at: 5946 5946 Size: 6283 BspHI T'CATG_A Cuts at: 3093 4172 5902 3093 Size: 1079 1730 3474 Fragments arranged by size: 3474 1730 1079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3638 844 Size: 2794 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4170 1394 Size: 429 2347 3507 Fragments arranged by size: 3507 2347 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2382 2830 3208 4778 4952 65 Size: 1183 1134 448 378 1570 174 1396 Fragments arranged by size: 1570 1396 1183 1134 448 378 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4927 5114 1700 Size: 110 3117 187 2869 Fragments arranged by size: 3117 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2142 96 Size: 2046 4237 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6283 BssSI C'ACGA_G Cuts at: 2446 4343 5355 2446 Size: 1897 1012 3374 Fragments arranged by size: 3374 1897 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6283 BstBI TT'CG_AA Cuts at: 5087 5087 Size: 6283 BstEII G'GTnAC_C Cuts at: 1184 3296 1184 Size: 2112 4171 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6283 BstYI r'GATC_y Cuts at: 1972 2334 2709 2904 3613 3980 4365 4382 5834 Size: 362 375 195 709 367 385 17 1452 Cuts at: 5834 5920 5932 6278 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 709 385 375 367 362 346 195 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3027 3027 Size: 6283 BtgI C'CryG_G Cuts at: 513 1065 2838 513 Size: 552 1773 3958 Fragments arranged by size: 3958 1773 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2504 487 Size: 683 56 1278 4266 Fragments arranged by size: 4266 1278 683 56 BtsI GCAGTG_nn' Cuts at: 2223 3799 4593 4613 2223 Size: 1576 794 20 3893 Fragments arranged by size: 3893 1576 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6283 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2598 2633 529 Size: 35 2034 35 4179 Fragments arranged by size: 4179 2034 35 35 DraI TTT'AAA Cuts at: 3587 3587 Size: 6283 DraIII CAC_nnn'GTG Cuts at: 2743 2743 Size: 6283 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5290 809 Size: 507 3974 1802 Fragments arranged by size: 3974 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2442 2583 3730 Size: 54 1425 50 275 18 612 141 1147 Cuts at: 3730 4619 6248 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1147 889 612 275 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3730 1537 Size: 275 1918 4090 Fragments arranged by size: 4090 1918 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2769 3015 3404 4213 1353 Size: 1416 246 389 809 3423 Fragments arranged by size: 3423 1416 809 389 246 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4870 5244 5390 387 Size: 1064 138 3281 374 146 1280 Fragments arranged by size: 3281 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2779 3157 2779 Size: 378 5905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2074 2174 2187 2330 2445 2698 2765 Size: 393 442 100 13 143 115 253 67 Cuts at: 2765 3173 3385 3452 4346 4943 5730 5998 1239 Size: 408 212 67 894 597 787 268 1524 Fragments arranged by size: 1524 894 787 597 442 408 393 268 253 212 143 115 100 67 67 13 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2603 2015 Size: 588 5695 EcoO109I rG'GnC_Cy Cuts at: 2104 3427 2104 Size: 1323 4960 EcoRI G'AATT_C Cuts at: 3592 3970 3592 Size: 378 5905 FspI TGC'GCA Cuts at: 3547 4789 3547 Size: 1242 5041 HaeII r_GCGC'y Cuts at: 1678 5430 1678 Size: 3752 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2630 2662 2896 Size: 32 135 32 524 32 502 32 234 Cuts at: 2896 2928 3125 3157 4924 4956 4998 5030 1373 Size: 32 197 32 1767 32 42 32 2626 Fragments arranged by size: 2626 1767 524 502 234 197 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3627 3873 669 Size: 988 281 1689 246 3079 Fragments arranged by size: 3079 1689 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6283 HpaI GTT'AAC Cuts at: 3873 3873 Size: 6283 KpnI G_GTAC'C Cuts at: 3608 3608 Size: 6283 MfeI C'AATT_G Cuts at: 3882 3882 Size: 6283 MluI A'CGCG_T Cuts at: 6159 6159 Size: 6283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2254 2973 5397 5581 1305 Size: 949 719 2424 184 2007 Fragments arranged by size: 2424 2007 949 719 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2444 2585 10 Size: 54 1425 343 612 141 3708 Fragments arranged by size: 3708 1425 612 343 141 54 MslI CAynn'nnrTG Cuts at: 518 1166 2450 2843 4241 4600 4759 518 Size: 648 1284 393 1398 359 159 2042 Fragments arranged by size: 2042 1398 1284 648 393 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 2320 2581 2678 2995 3699 4376 5524 Size: 448 266 261 97 317 704 677 1148 Cuts at: 5524 5769 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 704 677 448 317 266 261 245 97 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5731 NdeI CA'TA_TG Cuts at: 387 387 Size: 6283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6283 NotI GC'GGCC_GC Cuts at: 3730 3730 Size: 6283 NspI r_CATG'y Cuts at: 1132 1229 2143 3641 5186 1132 Size: 97 914 1498 1545 2229 Fragments arranged by size: 2229 1545 1498 914 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6283 PciI A'CATG_T Cuts at: 5182 5182 Size: 6283 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6283 PfoI T'CCnGG_A Cuts at: 2100 2114 3129 2100 Size: 14 1015 5254 Fragments arranged by size: 5254 1015 14 PmeI GTTT'AAAC Cuts at: 3587 3587 Size: 6283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4352 4384 5865 5897 4352 Size: 32 1481 32 4738 Fragments arranged by size: 4738 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 3427 2104 Size: 1323 4960 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6283 PsiI TTA'TAA Cuts at: 3853 3853 Size: 6283 PstI C_TGCA'G Cuts at: 830 3635 830 Size: 2805 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6283 PvuII CAG'CTG Cuts at: 2320 2581 2678 2995 2320 Size: 261 97 317 5608 Fragments arranged by size: 5608 317 261 97 SacI G_AGCT'C Cuts at: 2781 3159 2781 Size: 378 5905 SalI G'TCGA_C Cuts at: 1936 3625 1936 Size: 1689 4594 SapI GCTCTTCn'nnn_ Cuts at: 2769 3015 2769 Size: 246 6037 SbfI CC_TGCA'GG Cuts at: 3635 3635 Size: 6283 ScaI AGT'ACT Cuts at: 4531 6243 4531 Size: 1712 4571 SfcI C'TryA_G Cuts at: 826 1045 3118 3552 3631 4766 5447 5638 826 Size: 219 2073 434 79 1135 681 191 1471 Fragments arranged by size: 2073 1471 1135 681 434 219 191 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6283 SmaI CCC'GGG Cuts at: 1872 3109 3610 1872 Size: 1237 501 4545 Fragments arranged by size: 4545 1237 501 SmlI C'TyrA_G Cuts at: 820 1017 1943 3065 3371 4387 5288 5550 820 Size: 197 926 1122 306 1016 901 262 1553 Fragments arranged by size: 1553 1122 1016 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 4074 493 Size: 3581 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6283 SphI G_CATG'C Cuts at: 1132 3641 1132 Size: 2509 3774 SrfI GCCC'GGGC Cuts at: 3109 3109 Size: 6283 SspI AAT'ATT Cuts at: 5 52 4207 5 Size: 47 4155 2081 Fragments arranged by size: 4155 2081 47 StuI AGG'CCT Cuts at: 1517 2180 1517 Size: 663 5620 StyI C'CwwG_G Cuts at: 513 1065 2599 3301 3321 3722 513 Size: 552 1534 702 20 401 3074 Fragments arranged by size: 3074 1534 702 552 401 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2704 2710 3284 4310 4327 4480 4665 Size: 1255 1124 6 574 1026 17 153 185 Cuts at: 4665 5110 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1124 1026 574 445 185 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 3081 4529 Size: 274 80 33 51 1443 165 939 1448 Cuts at: 4529 6241 96 Size: 1712 138 Fragments arranged by size: 1712 1448 1443 939 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3482 3514 6243 6275 3482 Size: 32 2729 32 3490 Fragments arranged by size: 3490 2729 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6283 XbaI T'CTAG_A Cuts at: 3619 3619 Size: 6283 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6283 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6283 XmaI C'CCGG_G Cuts at: 1870 3107 3608 1870 Size: 1237 501 4545 Fragments arranged by size: 4545 1237 501 XmnI GAAnn'nnTTC Cuts at: 4412 4412 Size: 6283 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4091 276 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SwaI