Restriction map for
pFN21AE1456
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AccI AccII AccIII AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaWI BseAI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KasI KspI MaeI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 178 GACGTC GACGT^C
Acc65I 120 GGTACC G^GTACC
AcyI 178 GACGTC G[GA]^CG[CT]C
AflIII 212 ACATGT A^C[GA][CT]GT
AhdI 178 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 324 GATCC GGATC(4/5)
Asp718I 120 GGTACC G^GTACC
AspEI 178 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 208 GAGCAC G[AT]GC[AT]^C
AvaII 336 GGACC G^G[AT]CC
BanI 120 GGTACC G^G[CT][GA]CC
BglI 43 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 163 CTGGAG CTGGAG(16/14)
BsaAI 349 TACGTG [CT]AC^GT[GA]
BsaBI 265 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 178 GACGTC G[GA]^CG[CT]C
BsiHKAI 208 GAGCAC G[AT]GC[AT]^C
BsmBI 292 GAGACG CGTCTC(1/5)
BsmFI 335 GGGAC GGGAC(10/14)
BsmI 385 GAATGC GAATGC(1/-1)
BspHI 8 TCATGA T^CATGA
BspLU11I 212 ACATGT A^CATGT
BstF5I 224 CATCC GGATG(2/0)
BstXI 197 CCAGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 323 AGATCC [GA]^GATC[CT]
DdeI 368 CTCAG C^T[ACGT]AG
DpnII 324 GATC ^GATC
DraIII 277 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 178 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 444 GAAGAG CTCTTC(1/4)
EcoT14I 431 CCAAGG C^C[AT][AT]GG
FauI 399 GCGGG CCCGC(4/6)
FokI 224 CATCC GGATG(9/13)
Hin1I 178 GACGTC G[GA]^CG[CT]C
HinfI 270 GAATC G^A[ACGT]TC
HphI 305 TCACC GGTGA(8/7)
KpnI 120 GGTACC GGTAC^C
Ksp632I 444 GAAGAG CTCTTC(1/4)
MaeIII 304 GTCAC ^GT[ACGT]AC
MamI 265 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 324 GATC ^GATC
MflI 323 AGATCC [GA]^GATC[CT]
NaeI 389 GCCGGC GCC^GGC
NdeII 324 GATC ^GATC
NgoMIV 389 GCCGGC G^CCGGC
NspI 212 ACATGT [GA]CATG^[CT]
PstI 31 CTGCAG CTGCA^G
RcaI 8 TCATGA T^CATGA
Sau3AI 324 GATC ^GATC
SfaNI 493 GATGC GCATC(5/9)
SfcI 31 CTGCAG C^T[GA][CT]AG
StyI 431 CCAAGG C^C[AT][AT]GG
TfiI 270 GAATC G^A[AT]TC
Tsp45I 304 GTCAC ^GT[GC]AC
XhoII 323 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 121 GTAC GT^AC
192 GTAC
AlwNI 34 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
147 CAGCTACTG
BmyI 64 GTGCCC G[AGT]GC[ACT]^C
208 GAGCAC
BsaI 426 GAGACC GGTCTC(1/5)
465 GAGACC
BsaJI 431 CCAAGG C^C[ACGT][ACGT]GG
502 CCCCGG
BsgI 246 CTGCAC GTGCAG(16/14)
274 CTGCAC
Bsp1286I 64 GTGCCC G[AGT]GC[ACT]^C
208 GAGCAC
BstNI 39 CCTGG CC^[AT]GG
129 CCAGG
EaeI 41 TGGCCG [CT]^GGCC[GA]
296 CGGCCA
EcoRII 39 CCTGG ^CC[AT]GG
129 CCAGG
HaeII 51 GGCGCT [GA]GCGC^[CT]
159 GGCGCT
MboII 405 GAAGA GAAGA(8/7)
444 GAAGA
MvaI 39 CCTGG CC^[AT]GG
129 CCAGG
RsaI 121 GTAC GT^AC
192 GTAC
TspRI 76 CAGTG CA[GC]TG[ACGT][ACGT]^
277 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BcnI BseRI
BsiYI BslI BsmAI BsrFI BssKI
BstAPI Cac8I CfoI Cfr10I Cfr13I
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MnlI MspI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI TseI