Restriction map for
pFN21AE1456
(Circular) (Six-base) MAPSORT of: pFN21AE1456.seq Check: 2348 from: 1 to: 5215
pFN21AE1456 5215 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2187 3025 278
Size: 53 83 186 936 651 838 2468
Fragments arranged by size:
2468 936 838 651 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2558 1937
Size: 621 4594
Acc65I G'GTAC_C
Cuts at: 2125 2536 2125
Size: 411 4804
AclI AA'CG_TT
Cuts at: 1563 3342 3715 1563
Size: 1779 373 3063
Fragments arranged by size:
3063 1779 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3278 4662 4930 1632
Size: 1646 1384 268 1917
Fragments arranged by size:
1917 1646 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5018
AflIII A'CryG_T
Cuts at: 1203 2217 4114 5091 1203
Size: 1014 1897 977 1327
Fragments arranged by size:
1897 1327 1014 977
AgeI A'CCGG_T
Cuts at: 4046 4046
Size: 5215
AhdI GACnn_n'nnGTC
Cuts at: 2188 3944 2188
Size: 1756 3459
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5215
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 2351 2383 2204
Size: 32 115 32 5036
Fragments arranged by size:
5036 115 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2044 2157 2958 4530 2044
Size: 113 801 1572 2729
Fragments arranged by size:
2729 1572 801 113
ApaLI G'TGCA_C
Cuts at: 3272 4428 3272
Size: 1156 4059
ApoI r'AATT_y
Cuts at: 1493 2524 2750 2902 5011 1493
Size: 1031 226 152 2109 1697
Fragments arranged by size:
2109 1697 1031 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3769 160
Size: 872 2737 1606
Fragments arranged by size:
2737 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5215
AvaI C'yCGr_G
Cuts at: 1870 1943 2540 1870
Size: 73 597 4545
Fragments arranged by size:
4545 597 73
BamHI G'GATC_C
Cuts at: 2545 2912 2545
Size: 367 4848
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2125 2536 3991 618
Size: 325 217 627 338 411 1455 1842
Fragments arranged by size:
1842 1455 627 411 338 325 217
BanII G_rGCy'C
Cuts at: 1391 2534 1391
Size: 1143 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3406 3440 1903
Size: 34 50 34 1385 34 3678
Fragments arranged by size:
3678 1385 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3107 4317 3107
Size: 1210 4005
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5215
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 3826 136
Size: 107 122 71 631 605 382 1772 1525
Fragments arranged by size:
1772 1525 631 605 382 122 107 71
BglII A'GATC_T
Cuts at: 5210 5210
Size: 5215
BlpI GC'TnA_GC
Cuts at: 2632 2632
Size: 5215
Bme1580I G_kGCm'C
Cuts at: 1790 2073 3276 4432 1790
Size: 283 1203 1156 2573
Fragments arranged by size:
2573 1203 1156 283
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5215
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2946 3904 442
Size: 416 53 83 399 24 1529 958 1753
Fragments arranged by size:
1753 1529 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 3875 1239
Size: 950 1686 2579
Fragments arranged by size:
2579 1686 950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3340 4205 4503 3340
Size: 865 298 4052
Fragments arranged by size:
4052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2425 2464 3878 882
Size: 617 926 39 1414 2219
Fragments arranged by size:
2219 1414 926 617 39
BsaAI yAC'GTr
Cuts at: 493 1204 2356 3006 493
Size: 711 1152 650 2702
Fragments arranged by size:
2702 1152 711 650
BsaBI GATnn'nnATC
Cuts at: 2274 5209 2274
Size: 2935 2280
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 3022 3404 4039
Size: 53 83 186 936 651 838 382 635
Cuts at: 4039 275
Size: 1451
Fragments arranged by size:
1451 936 838 651 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3648 4046 4320 4467 4878 3648
Size: 398 274 147 411 3985
Fragments arranged by size:
3985 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 2333 2351 2363
Size: 30 165 30 801 30 99 18 12
Cuts at: 2363 2381 1178
Size: 18 4012
Fragments arranged by size:
4012 801 165 99 30 30 30 18
18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2443 2467 2470 1186
Size: 1257 24 3 3931
Fragments arranged by size:
3931 1257 24 3
BseYI C'CCAG_C
Cuts at: 2076 2096 2201 4418 4914 2076
Size: 20 105 2217 496 2377
Fragments arranged by size:
2377 2217 496 105 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2236 2264 1163
Size: 573 500 28 4114
Fragments arranged by size:
4114 573 500 28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2665 3426 4454 1540
Size: 275 186 664 761 1028 2301
Fragments arranged by size:
2301 1028 761 664 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 3276 3361 4432 2217
Size: 1059 85 1071 3000
Fragments arranged by size:
3000 1071 1059 85
BsmI GAATG_Cn'
Cuts at: 2396 2726 2819 2396
Size: 330 93 4792
Fragments arranged by size:
4792 330 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2291 2291
Size: 5215
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2217 2534 3276 3361 4432 1391
Size: 399 283 144 317 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 317 283 144 85
BspEI T'CCGG_A
Cuts at: 4878 4878
Size: 5215
BspHI T'CATG_A
Cuts at: 2013 3104 4834 2013
Size: 1091 1730 2394
Fragments arranged by size:
2394 1730 1091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2570 844
Size: 1726 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3102 1394
Size: 429 1279 3507
Fragments arranged by size:
3507 1279 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3710 3884 65
Size: 1183 2462 174 1396
Fragments arranged by size:
2462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2255 2290 2343 2394 3859 4046
Size: 110 198 247 35 53 51 1465 187
Cuts at: 4046 1700
Size: 2869
Fragments arranged by size:
2869 1465 247 198 187 110 53 51
35
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5215
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5215
BssSI C'ACGA_G
Cuts at: 3275 4287 3275
Size: 1012 4203
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2157 2259 2287 794
Size: 1363 102 28 3722
Fragments arranged by size:
3722 1363 102 28
BstBI TT'CG_AA
Cuts at: 4019 4019
Size: 5215
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5215
BstXI CCAn_nnnn'nTGG
Cuts at: 2209 2209
Size: 5215
BstYI r'GATC_y
Cuts at: 1972 2328 2545 2912 3297 3314 4766 4852 4864
Size: 356 217 367 385 17 1452 86 12
Cuts at: 4864 5210 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 356 346 217 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4663
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2283 2511 487
Size: 683 56 1057 228 3191
Fragments arranged by size:
3191 1057 683 228 56
BtsI GCAGTG_nn'
Cuts at: 2087 2731 3525 3545 2087
Size: 644 794 20 3757
Fragments arranged by size:
3757 794 644 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5180
DraI TTT'AAA
Cuts at: 2519 2519
Size: 5215
DraIII CAC_nnn'GTG
Cuts at: 2287 2287
Size: 5215
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4222 809
Size: 507 2906 1802
Fragments arranged by size:
2906 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2301 2662
Size: 54 1425 50 275 18 216 255 361
Cuts at: 2662 3551 5180 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 361 275 255 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2662 1537
Size: 275 850 4090
Fragments arranged by size:
4090 850 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 3145 1353
Size: 1091 701 3423
Fragments arranged by size:
3423 1091 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3802 4176 4322 387
Size: 1064 138 2213 374 146 1280
Fragments arranged by size:
2213 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 3278 3875 4662 4930 1239
Size: 393 557 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 787 597 557 393 268
EcoRI G'AATT_C
Cuts at: 2524 2902 2524
Size: 378 4837
FspI TGC'GCA
Cuts at: 3721 3721
Size: 5215
HaeII r_GCGC'y
Cuts at: 1678 2060 2168 4362 1678
Size: 382 108 2194 2531
Fragments arranged by size:
2531 2194 382 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 2333 2356 2365
Size: 32 135 32 602 32 127 23 9
Cuts at: 2365 2388 3856 3888 3930 3962 1373
Size: 23 1468 32 42 32 2626
Fragments arranged by size:
2626 1468 602 135 127 42 32 32
32 32 32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2559 2805 669
Size: 988 281 621 246 3079
Fragments arranged by size:
3079 988 621 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5215
HpaI GTT'AAC
Cuts at: 2805 2805
Size: 5215
KpnI G_GTAC'C
Cuts at: 2129 2540 2129
Size: 411 4804
MfeI C'AATT_G
Cuts at: 2814 2814
Size: 5215
MluI A'CGCG_T
Cuts at: 5091 5091
Size: 5215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4329 4513 1305
Size: 3024 184 2007
Fragments arranged by size:
3024 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3393
Fragments arranged by size:
3393 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3173 3532 3691 518
Size: 648 2007 359 159 2042
Fragments arranged by size:
2042 2007 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2631 3308 4456 4701 1606
Size: 1025 677 1148 245 2120
Fragments arranged by size:
2120 1148 1025 677 245
NaeI GCC'GGC
Cuts at: 2396 2396
Size: 5215
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4663
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5215
NgoMIV G'CCGG_C
Cuts at: 2394 2394
Size: 5215
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5215
NotI GC'GGCC_GC
Cuts at: 2662 2662
Size: 5215
NspI r_CATG'y
Cuts at: 1132 1229 2221 2573 4118 1132
Size: 97 992 352 1545 2229
Fragments arranged by size:
2229 1545 992 352 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5215
PciI A'CATG_T
Cuts at: 2217 4114 2217
Size: 1897 3318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5215
PfoI T'CCnGG_A
Cuts at: 2133 2133
Size: 5215
PmeI GTTT'AAAC
Cuts at: 2519 2519
Size: 5215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2204 2236 2351 2383 3284 3316 4797 4829 2204
Size: 32 115 32 901 32 1481 32 2590
Fragments arranged by size:
2590 1481 901 115 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5215
PsiI TTA'TAA
Cuts at: 2785 2785
Size: 5215
PstI C_TGCA'G
Cuts at: 830 2040 2567 830
Size: 1210 527 3478
Fragments arranged by size:
3478 1210 527
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5215
SalI G'TCGA_C
Cuts at: 1936 2557 1936
Size: 621 4594
SbfI CC_TGCA'GG
Cuts at: 2567 2567
Size: 5215
ScaI AGT'ACT
Cuts at: 3463 5175 3463
Size: 1712 3503
SfcI C'TryA_G
Cuts at: 826 1045 2036 2563 3698 4379 4570 826
Size: 219 991 527 1135 681 191 1471
Fragments arranged by size:
1471 1135 991 681 527 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5215
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5215
SmaI CCC'GGG
Cuts at: 1872 2542 1872
Size: 670 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3319 4220 4482 820
Size: 197 926 1376 901 262 1553
Fragments arranged by size:
1553 1376 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3006 493
Size: 2513 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5215
SphI G_CATG'C
Cuts at: 1132 2573 1132
Size: 1441 3774
SspI AAT'ATT
Cuts at: 5 52 3139 5
Size: 47 3087 2081
Fragments arranged by size:
3087 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5215
StyI C'CwwG_G
Cuts at: 513 1065 2436 2654 513
Size: 552 1371 218 3074
Fragments arranged by size:
3074 1371 552 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3242 3259 3412 3597 4042 325
Size: 1255 1662 17 153 185 445 1498
Fragments arranged by size:
1662 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3461 5173 96
Size: 274 80 33 51 1443 1484 1712 138
Fragments arranged by size:
1712 1484 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5175 5207 5175
Size: 32 5183
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5215
XbaI T'CTAG_A
Cuts at: 2551 2551
Size: 5215
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5215
XmaI C'CCGG_G
Cuts at: 1870 2540 1870
Size: 670 4545
XmnI GAAnn'nnTTC
Cuts at: 3344 3344
Size: 5215
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2185 3023 276
Size: 53 83 186 936 651 838 2468
Fragments arranged by size:
2468 936 838 651 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI