Restriction map for
pFN21AE1004
(Circular) (Six-base) MAPSORT of: pFN21AE1004.seq Check: 2364 from: 1 to: 5191
pFN21AE1004 5191 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3001 278
Size: 53 83 186 936 1465 2468
Fragments arranged by size:
2468 1465 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2534 1937
Size: 597 4594
Acc65I G'GTAC_C
Cuts at: 2512 2512
Size: 5191
AclI AA'CG_TT
Cuts at: 1563 3318 3691 1563
Size: 1755 373 3063
Fragments arranged by size:
3063 1755 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3254 4638 4906 1632
Size: 1622 1384 268 1917
Fragments arranged by size:
1917 1622 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4994
AflIII A'CryG_T
Cuts at: 1203 4090 5067 1203
Size: 2887 977 1327
Fragments arranged by size:
2887 1327 977
AgeI A'CCGG_T
Cuts at: 4022 4022
Size: 5191
AhdI GACnn_n'nnGTC
Cuts at: 3920 3920
Size: 5191
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2088 2120 2088
Size: 32 5159
AlwNI CAG_nnn'CTG
Cuts at: 2073 2138 2934 4506 2073
Size: 65 796 1572 2758
Fragments arranged by size:
2758 1572 796 65
ApaLI G'TGCA_C
Cuts at: 3248 4404 3248
Size: 1156 4035
ApoI r'AATT_y
Cuts at: 1493 2500 2726 2878 4987 1493
Size: 1007 226 152 2109 1697
Fragments arranged by size:
2109 1697 1007 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3745 160
Size: 872 2713 1606
Fragments arranged by size:
2713 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5191
AvaI C'yCGr_G
Cuts at: 1870 1943 2516 1870
Size: 73 573 4545
Fragments arranged by size:
4545 573 73
BamHI G'GATC_C
Cuts at: 2521 2888 2521
Size: 367 4824
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2512 3967 618
Size: 325 217 627 725 1455 1842
Fragments arranged by size:
1842 1455 725 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2510 1391
Size: 1119 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3382 3416 1903
Size: 34 50 34 1361 34 3678
Fragments arranged by size:
3678 1361 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3083 4293 3083
Size: 1210 3981
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5191
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3802 136
Size: 107 122 71 631 605 2130 1525
Fragments arranged by size:
2130 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5186 5186
Size: 5191
BlpI GC'TnA_GC
Cuts at: 2608 2608
Size: 5191
Bme1580I G_kGCm'C
Cuts at: 1790 3252 4408 1790
Size: 1462 1156 2573
Fragments arranged by size:
2573 1462 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5191
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 2922 3880
Size: 416 53 83 399 24 666 839 958
Cuts at: 3880 442
Size: 1753
Fragments arranged by size:
1753 958 839 666 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 3851 1239
Size: 816 1796 2579
Fragments arranged by size:
2579 1796 816
Bpu10I CC'TnA_GC
Cuts at: 2028 2028
Size: 5191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2452 3316 4181 4479 2452
Size: 864 865 298 3164
Fragments arranged by size:
3164 865 864 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2031 3854 882
Size: 617 532 1823 2219
Fragments arranged by size:
2219 1823 617 532
BsaAI yAC'GTr
Cuts at: 493 1204 2982 493
Size: 711 1778 2702
Fragments arranged by size:
2702 1778 711
BsaBI GATnn'nnATC
Cuts at: 5185 5185
Size: 5191
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2998 3380 4015 275
Size: 53 83 186 936 1465 382 635 1451
Fragments arranged by size:
1465 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3624 4022 4296 4443 4854 3624
Size: 398 274 147 411 3961
Fragments arranged by size:
3961 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4966
Fragments arranged by size:
4966 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 1186
Size: 1123 4068
BseYI C'CCAG_C
Cuts at: 4394 4890 4394
Size: 496 4695
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2163 1163
Size: 573 427 4191
Fragments arranged by size:
4191 573 427
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2641 3402 4430 1540
Size: 275 186 640 761 1028 2301
Fragments arranged by size:
2301 1028 761 640 275 186
BsiHKAI G_wGCw'C
Cuts at: 2319 2349 3252 3337 4408 2319
Size: 30 903 85 1071 3102
Fragments arranged by size:
3102 1071 903 85 30
BsmI GAATG_Cn'
Cuts at: 2702 2795 2702
Size: 93 5098
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2319 2349 2510 3252 3337 4408 1391
Size: 399 529 30 161 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 529 399 161 85 30
BspEI T'CCGG_A
Cuts at: 4854 4854
Size: 5191
BspHI T'CATG_A
Cuts at: 3080 4810 3080
Size: 1730 3461
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2546 844
Size: 1702 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3078 1394
Size: 429 1255 3507
Fragments arranged by size:
3507 1255 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3686 3860 65
Size: 1183 2438 174 1396
Fragments arranged by size:
2438 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3835 4022 1700
Size: 110 2025 187 2869
Fragments arranged by size:
2869 2025 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5191
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5191
BssSI C'ACGA_G
Cuts at: 3251 4263 3251
Size: 1012 4179
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 2420 794
Size: 1520 106 3565
Fragments arranged by size:
3565 1520 106
BstBI TT'CG_AA
Cuts at: 3995 3995
Size: 5191
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5191
BstYI r'GATC_y
Cuts at: 1972 2521 2888 3273 3290 4742 4828 4840 5186
Size: 549 367 385 17 1452 86 12 346
Cuts at: 5186 1972
Size: 1977
Fragments arranged by size:
1977 1452 549 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4639
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2106 2392 487
Size: 683 56 880 286 3286
Fragments arranged by size:
3286 880 683 286 56
BtsI GCAGTG_nn'
Cuts at: 2389 2707 3501 3521 2389
Size: 318 794 20 4059
Fragments arranged by size:
4059 794 318 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5191
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5156
DraI TTT'AAA
Cuts at: 2495 2495
Size: 5191
DraIII CAC_nnn'GTG
Cuts at: 2353 2353
Size: 5191
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4198 809
Size: 507 2882 1802
Fragments arranged by size:
2882 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2638 3527
Size: 54 1425 50 275 18 378 430 889
Cuts at: 3527 5156 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 430 378 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2638 1537
Size: 275 826 4090
Fragments arranged by size:
4090 826 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3121 1353
Size: 1768 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2149 2306 3778 4152 4298 387
Size: 1064 138 560 157 1472 374 146 1280
Fragments arranged by size:
1472 1280 1064 560 374 157 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 3254 3851 4638 4906 1239
Size: 393 423 1199 597 787 268 1524
Fragments arranged by size:
1524 1199 787 597 423 393 268
EcoRI G'AATT_C
Cuts at: 2500 2878 2500
Size: 378 4813
FspI TGC'GCA
Cuts at: 3697 3697
Size: 5191
HaeII r_GCGC'y
Cuts at: 1678 4338 1678
Size: 2660 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 2388 2420 3832
Size: 32 135 32 668 32 116 32 1412
Cuts at: 3832 3864 3906 3938 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1412 668 135 116 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2233 2535 2781 669
Size: 988 281 295 302 246 3079
Fragments arranged by size:
3079 988 302 295 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5191
HpaI GTT'AAC
Cuts at: 2781 2781
Size: 5191
KpnI G_GTAC'C
Cuts at: 2516 2516
Size: 5191
MfeI C'AATT_G
Cuts at: 2790 2790
Size: 5191
MluI A'CGCG_T
Cuts at: 5067 5067
Size: 5191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4305 4489 1305
Size: 3000 184 2007
Fragments arranged by size:
3000 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3369
Fragments arranged by size:
3369 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3149 3508 3667 518
Size: 648 1983 359 159 2042
Fragments arranged by size:
2042 1983 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2263 2287 2607 3284 4432 4677 1606
Size: 657 24 320 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 657 320 245 24
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4639
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5191
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5191
NotI GC'GGCC_GC
Cuts at: 2638 2638
Size: 5191
NspI r_CATG'y
Cuts at: 1132 1229 2549 4094 1132
Size: 97 1320 1545 2229
Fragments arranged by size:
2229 1545 1320 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5191
PciI A'CATG_T
Cuts at: 4090 4090
Size: 5191
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5191
PmeI GTTT'AAAC
Cuts at: 2495 2495
Size: 5191
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 4773 4805 3260
Size: 32 1481 32 3646
Fragments arranged by size:
3646 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5191
PsiI TTA'TAA
Cuts at: 2761 2761
Size: 5191
PstI C_TGCA'G
Cuts at: 830 2273 2543 830
Size: 1443 270 3478
Fragments arranged by size:
3478 1443 270
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5191
SalI G'TCGA_C
Cuts at: 1936 2533 1936
Size: 597 4594
SbfI CC_TGCA'GG
Cuts at: 2543 2543
Size: 5191
ScaI AGT'ACT
Cuts at: 2255 3439 5151 2255
Size: 1184 1712 2295
Fragments arranged by size:
2295 1712 1184
SexAI A'CCwGG_T
Cuts at: 2226 2388 2226
Size: 162 5029
SfcI C'TryA_G
Cuts at: 826 1045 2065 2269 2539 3674 4355 4546 826
Size: 219 1020 204 270 1135 681 191 1471
Fragments arranged by size:
1471 1135 1020 681 270 219 204 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5191
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5191
SmaI CCC'GGG
Cuts at: 1872 2518 1872
Size: 646 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2467 3295 4196 4458 820
Size: 197 926 524 828 901 262 1553
Fragments arranged by size:
1553 926 901 828 524 262 197
SnaBI TAC'GTA
Cuts at: 493 2982 493
Size: 2489 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5191
SphI G_CATG'C
Cuts at: 1132 2549 1132
Size: 1417 3774
SspI AAT'ATT
Cuts at: 5 52 3115 5
Size: 47 3063 2081
Fragments arranged by size:
3063 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5191
StyI C'CwwG_G
Cuts at: 513 1065 2630 513
Size: 552 1565 3074
Fragments arranged by size:
3074 1565 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3218 3235 3388 3573 4018 325
Size: 1255 1638 17 153 185 445 1498
Fragments arranged by size:
1638 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 3437 5149
Size: 274 80 33 51 1443 276 1184 1712
Cuts at: 5149 96
Size: 138
Fragments arranged by size:
1712 1443 1184 276 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5151 5183 5151
Size: 32 5159
Tth111I GACn'n_nGTC
Cuts at: 1316 2390 1316
Size: 1074 4117
XbaI T'CTAG_A
Cuts at: 2527 2527
Size: 5191
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5191
XmaI C'CCGG_G
Cuts at: 1870 2516 1870
Size: 646 4545
XmnI GAAnn'nnTTC
Cuts at: 3320 3320
Size: 5191
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2999 276
Size: 53 83 186 936 1465 2468
Fragments arranged by size:
2468 1465 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI