################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AflII AgeI Alw44I Aor51HI ApaLI AscI Asp718I AspI AviII AvrII BbeI BbrPI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsmBI BsmI Bsp1407I BspDI BspEI BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI FspI HaeII HincII HindII HpaI KasI KpnI Ksp632I KspI MluI MroI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AclI 974 AACGTT AA^CGTT AfaI 328 GTAC GT^AC AflIII 131 ACATGT A^C[GA][CT]GT AhdI 3 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 19 CCTCAGG CC^T[ACGT]AGG ApaI 1252 GGGCCC GGGCC^C AseI 448 ATTAAT AT^TAAT AsnI 448 ATTAAT AT^TAAT Asp700I 87 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 727 CCCGGG C^[CT]CG[GA]G BanI 460 GGTGCC G^G[CT][GA]CC BanII 1252 GGGCCC G[GA]GC[CT]^C BbsI 1165 GTCTTC GAAGAC(2/6) BbvCI 1273 GCTGAGG CCTCAGC(-5/-2) BfaI 544 CTAG C^TAG BpmI 797 CTGGAG CTGGAG(16/14) BpuAI 1165 GTCTTC GAAGAC(2/6) BsaBI 432 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 769 GAGGAG GAGGAG(10/8) BsgI 394 GTGCAG GTGCAG(16/14) BsiWI 327 CGTACG C^GTACG BsmFI 135 GTCCC GGGAC(10/14) BsoBI 727 CCCGGG C^[CT]CG[GA]G BspLU11I 131 ACATGT A^CATGT BsrBI 214 GAGCGG CCGCTC(-3/-3) BstXI 353 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 19 CCTCAGG CC^T[ACGT]AGG CfoI 578 GCGC GCG^C Eam1105I 3 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 19 CCTCAGG CC^T[ACGT]AGG EcoNI 384 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 688 GAATTC G^AATTC EcoT14I 149 CCTTGG C^C[AT][AT]GG HhaI 578 GCGC GCG^C HinP1I 578 GCGC G^CGC HindIII 715 AAGCTT A^AGCTT HinfI 793 GAATC G^A[ACGT]TC MaeI 544 CTAG C^TAG MaeII 975 ACGT A^CGT MamI 432 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1361 CAATTG C^AATTG MunI 1361 CAATTG C^AATTG PpuMI 723 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1158 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 448 ATTAAT AT^TAAT Psp1406I 974 AACGTT AA^CGTT RsaI 328 GTAC GT^AC SbfI 468 CCTGCAGG CCTGCA^GG SmaI 727 CCCGGG CCC^GGG SmlI 712 CTCAAG C^T[CT][GA]AG Sse8387I 468 CCTGCAGG CCTGCA^GG StyI 149 CCTTGG C^C[AT][AT]GG TaqI 1169 TCGA T^CGA TfiI 793 GAATC G^A[AT]TC TthHB8I 1169 TCGA T^CGA XmaCI 727 CCCGGG C^CCGGG XmaI 727 CCCGGG C^CCGGG XmnI 87 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 87 GAATTT [GA]^AATT[CT] 688 GAATTC AcyI 175 GACGCC G[GA]^CG[CT]C 183 GGCGTC ApoI 87 GAATTT [GA]^AATT[CT] 688 GAATTC AspHI 442 GTGCTC G[AT]GC[AT]^C 1171 GAGCAC AvaII 724 GGACC G^G[AT]CC 750 GGACC BamHI 314 GGATCC G^GATCC 332 GGATCC BcgI 1170 CGAGCACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1241 GCATGTGCTTCG BclI 371 TGATCA T^GATCA 431 TGATCA BsaHI 175 GACGCC G[GA]^CG[CT]C 183 GGCGTC BsaI 739 GAGACC GGTCTC(1/5) 1282 GAGACC BsiHKAI 442 GTGCTC G[AT]GC[AT]^C 1171 GAGCAC BstYI 314 GGATCC [GA]^GATC[CT] 332 GGATCC DraII 539 AGGGCCT [GA]G^G[ACGT]CC[CT] 723 AGGACCC DsaI 82 CCGTGG C^C[GA][CT]GG 563 CCGTGG EcoO109I 539 AGGGCCT [GA]G^G[ACGT]CC[CT] 723 AGGACCC FauI 680 CCCGC CCCGC(4/6) 1145 CCCGC FbaI 371 TGATCA T^GATCA 431 TGATCA HgaI 175 GACGC GACGC(5/10) 184 GCGTC Hin1I 175 GACGCC G[GA]^CG[CT]C 183 GGCGTC MflI 314 GGATCC [GA]^GATC[CT] 332 GGATCC MseI 449 TTAA T^TAA 1139 TTAA NspI 131 ACATGT [GA]CATG^[CT] 1241 GCATGT PstI 197 CTGCAG CTGCA^G 469 CTGCAG SfcI 197 CTGCAG C^T[GA][CT]AG 469 CTGCAG Tru9I 449 TTAA T^TAA 1139 TTAA XhoII 314 GGATCC [GA]^GATC[CT] 332 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwI AlwNI BalI BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BspHI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII RcaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI