Restriction map for pFN21AE4439
 (Linear) (Six-base) MAPSORT of: KIEE4439.seq  Check: 4944  from: 1  to: 1368



KIEE4439.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:01  ..

AclI AA'CG_TT

Cuts at:      0     975    1368 
   Size:        975     393
 
AflIII A'CryG_T

Cuts at:      0     131    1368 
   Size:        131    1237
 
AhdI GACnn_n'nnGTC

Cuts at:      0       8    1368 
   Size:          8    1360
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     419    1368 
   Size:        419     949
 
AlwNI CAG_nnn'CTG

Cuts at:      0     968    1076    1238    1368 
   Size:        968     108     162     130

  Fragments arranged by size: 

                 968     162     130     108
 
ApaI G_GGCC'C

Cuts at:      0    1256    1368 
   Size:       1256     112
 
ApoI r'AATT_y

Cuts at:      0      87     688    1368 
   Size:         87     601     680

  Fragments arranged by size: 

                 680     601      87
 
AseI AT'TA_AT

Cuts at:      0     449    1368 
   Size:        449     919
 
AvaI C'yCGr_G

Cuts at:      0     727    1368 
   Size:        727     641
 
BamHI G'GATC_C

Cuts at:      0     314     332    1368 
   Size:        314      18    1036

  Fragments arranged by size: 

                1036     314      18
 
BanI G'GyrC_C

Cuts at:      0     460    1368 
   Size:        460     908
 
BanII G_rGCy'C

Cuts at:      0    1256    1368 
   Size:       1256     112
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1158    1368 
   Size:       1158     210
 
BbvCI CC'TCA_GC

Cuts at:      0    1274    1368 
   Size:       1274      94
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1159    1193    1230    1264    1368 
   Size:       1159      34      37      34     104

  Fragments arranged by size: 

                1159     104      37      34      34
 
BclI T'GATC_A

Cuts at:      0     371     431    1368 
   Size:        371      60     937

  Fragments arranged by size: 

                 937     371      60
 
BglI GCCn_nnn'nGGC

Cuts at:      0     548     565    1300    1368 
   Size:        548      17     735      68

  Fragments arranged by size: 

                 735     548      68      17
 
Bme1580I G_kGCm'C

Cuts at:      0    1256    1368 
   Size:       1256     112
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     315    1339    1368 
   Size:        315    1024      29

  Fragments arranged by size: 

                1024     315      29
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     237    1368 
   Size:        237    1131
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     818    1368 
   Size:        818     550
 
Bpu10I CC'TnA_GC

Cuts at:      0     212    1274    1368 
   Size:        212    1062      94

  Fragments arranged by size: 

                1062     212      94
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     697    1368 
   Size:        697     671
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     733    1276    1368 
   Size:        733     543      92

  Fragments arranged by size: 

                 733     543      92
 
BsaBI GATnn'nnATC

Cuts at:      0     436    1368 
   Size:        436     932
 
BsaHI Gr'CG_yC

Cuts at:      0     176     184    1368 
   Size:        176       8    1184

  Fragments arranged by size: 

                1184     176       8
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     280     310     730     760     791     821    1368 
   Size:        280      30     420      30      31      30     547

  Fragments arranged by size: 

                 547     420     280      31      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     784    1368 
   Size:        784     584
 
BseYI C'CCAG_C

Cuts at:      0      60    1368 
   Size:         60    1308
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415    1368 
   Size:        415     953
 
BsiHKAI G_wGCw'C

Cuts at:      0     446    1175    1368 
   Size:        446     729     193

  Fragments arranged by size: 

                 729     446     193
 
BsiWI C'GTAC_G

Cuts at:      0     327    1368 
   Size:        327    1041
 
Bsp1286I G_dGCh'C

Cuts at:      0     446    1175    1256    1368 
   Size:        446     729      81     112

  Fragments arranged by size: 

                 729     446     112      81
 
BspHI T'CATG_A

Cuts at:      0      95     248     527    1368 
   Size:         95     153     279     841

  Fragments arranged by size: 

                 841     279     153      95
 
BsrBI CCG'CTC

Cuts at:      0     216    1368 
   Size:        216    1152
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     360    1368 
   Size:        360    1008
 
BstYI r'GATC_y

Cuts at:      0     314     332    1368 
   Size:        314      18    1036

  Fragments arranged by size: 

                1036     314      18
 
Bsu36I CC'TnA_GG

Cuts at:      0      20    1368 
   Size:         20    1348
 
BtgI C'CryG_G

Cuts at:      0      82     563    1368 
   Size:         82     481     805

  Fragments arranged by size: 

                 805     481      82
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     936    1368 
   Size:        936     432
 
BtsI GCAGTG_nn'

Cuts at:      0     155     374    1117    1368 
   Size:        155     219     743     251

  Fragments arranged by size: 

                 743     251     219     155
 
EaeI y'GGCC_r

Cuts at:      0     101     350     534     566    1368 
   Size:        101     249     184      32     802

  Fragments arranged by size: 

                 802     249     184     101      32
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     818    1368 
   Size:        818     550
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     388    1368 
   Size:        388     980
 
EcoO109I rG'GnC_Cy

Cuts at:      0     540     724    1368 
   Size:        540     184     644

  Fragments arranged by size: 

                 644     540     184
 
EcoRI G'AATT_C

Cuts at:      0     688    1368 
   Size:        688     680
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26      90     122     791     823     928     960    1368 
   Size:         26      64      32     669      32     105      32     408

  Fragments arranged by size: 

                 669     408     105      64      32      32      32      26
            
 
HindIII A'AGCT_T

Cuts at:      0     715    1368 
   Size:        715     653
 
MfeI C'AATT_G

Cuts at:      0    1361    1368 
   Size:       1361       7
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     728     836    1241    1368 
   Size:        728     108     405     127

  Fragments arranged by size: 

                 728     405     127     108
 
MscI TGG'CCA

Cuts at:      0     352     536     568    1368 
   Size:        352     184      32     800

  Fragments arranged by size: 

                 800     352     184      32
 
MslI CAynn'nnrTG

Cuts at:      0     385     427     913     988    1368 
   Size:        385      42     486      75     380

  Fragments arranged by size: 

                 486     385     380      75      42
 
MspA1I CmG'CkG

Cuts at:      0     399    1045    1076    1273    1368 
   Size:        399     646      31     197      95

  Fragments arranged by size: 

                 646     399     197      95      31
 
NspI r_CATG'y

Cuts at:      0     135    1245    1368 
   Size:        135    1110     123

  Fragments arranged by size: 

                1110     135     123
 
PciI A'CATG_T

Cuts at:      0     131    1368 
   Size:        131    1237
 
PpuMI rG'GwC_Cy

Cuts at:      0     724    1368 
   Size:        724     644
 
PshAI GACnn'nnGTC

Cuts at:      0    1162    1368 
   Size:       1162     206
 
PspOMI G'GGCC_C

Cuts at:      0    1252    1368 
   Size:       1252     116
 
PstI C_TGCA'G

Cuts at:      0     201     473    1368 
   Size:        201     272     895

  Fragments arranged by size: 

                 895     272     201
 
PvuII CAG'CTG

Cuts at:      0     399    1045    1273    1368 
   Size:        399     646     228      95

  Fragments arranged by size: 

                 646     399     228      95
 
SbfI CC_TGCA'GG

Cuts at:      0     473    1368 
   Size:        473     895
 
SfcI C'TryA_G

Cuts at:      0     197     469    1368 
   Size:        197     272     899

  Fragments arranged by size: 

                 899     272     197
 
SmaI CCC'GGG

Cuts at:      0     729    1368 
   Size:        729     639
 
SmlI C'TyrA_G

Cuts at:      0     712    1368 
   Size:        712     656
 
StyI C'CwwG_G

Cuts at:      0     149    1368 
   Size:        149    1219
 
XmaI C'CCGG_G

Cuts at:      0     727    1368 
   Size:        727     641
 
XmnI GAAnn'nnTTC

Cuts at:      0      91    1368 
   Size:         91    1277
 

 Enzymes that do cut:

     AclI   AflIII     AhdI     AlfI    AlwNI     ApaI     ApoI     AseI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI     BclI
     BglI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI
 Bsp1286I    BspHI    BsrBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     EaeI  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I  HindIII
     MfeI     MmeI     MscI     MslI   MspA1I     NspI     PciI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SbfI     SfcI     SmaI     SmlI
     StyI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII     AgeI
     AleI     AloI    ApaLI     AscI    AsiSI    AvrII     BaeI     BbeI
    BciVI    BfrBI    BglII     BlpI    BmgBI     BmtI    BsaAI    BsaWI
    BsiEI     BsmI    BsmBI    BspEI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     ZraI
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