################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspI AvaII AviII BalI BbeI BbrPI BbsI BbvCI BclI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 1064 GGTACC G^GTACC AccII 1041 CGCG CG^CG AflIII 868 ACGTGT A^C[GA][CT]GT AhdI 778 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 791 GGGCCC GGGCC^C Asp718I 1064 GGTACC G^GTACC AspEI 778 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 264 CCTAGG C^CTAGG BamHI 313 GGATCC G^GATCC BcgI 849 CGAGGACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 433 GGATAC GTATCC(6/5) BlnI 264 CCTAGG C^CTAGG BsaBI 250 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1036 GAGACC GGTCTC(1/5) BseRI 874 GAGGAG GAGGAG(10/8) BsgI 236 GTGCAG GTGCAG(16/14) BsrBI 426 GAGCGG CCGCTC(-3/-3) BsrDI 855 CATTGC GCAATG(2/0) BssSI 400 CTCGTG CACGAG(-5/-1) BstNI 9 CCTGG CC^[AT]GG BstUI 1041 CGCG CG^CG DraII 791 GGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 1076 TGGCCG [CT]^GGCC[GA] Eam1105I 778 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1189 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 791 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 145 GAATTC G^AATTC EcoRII 9 CCTGG ^CC[AT]GG HapII 789 CCGG C^CGG HinfI 739 GAGTC G^A[ACGT]TC HpaII 789 CCGG C^CGG KpnI 1064 GGTACC GGTAC^C MaeII 868 ACGT A^CGT MaeIII 167 GTCAC ^GT[ACGT]AC MamI 250 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 185 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 653 CAGCTG C[AC]G^C[GT]G MspI 789 CCGG C^CGG MvaI 9 CCTGG CC^[AT]GG MvnI 1041 CGCG CG^CG PflMI 157 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 739 GAGTC GAGTC(4/5) PstI 929 CTGCAG CTGCA^G PvuII 653 CAGCTG CAG^CTG SacI 1159 GAGCTC GAGCT^C SmaI 788 CCCGGG CCC^GGG Tsp45I 167 GTCAC ^GT[GC]AC Van91I 157 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 788 CCCGGG C^CCGGG XmaI 788 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 32 GTAC GT^AC 1065 GTAC Asp700I 112 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 952 GAAGTTCTTC AvaI 415 CTCGGG C^[CT]CG[GA]G 788 CCCGGG BanI 1064 GGTACC G^G[CT][GA]CC 1185 GGCACC BcnI 788 CCCGG CC^[GC]GG 789 CCGGG BpmI 923 CTGGAG CTGGAG(16/14) 1016 CTGGAG BsmAI 1036 GAGAC GTCTC(1/5) 741 GTCTC BsoBI 415 CTCGGG C^[CT]CG[GA]G 788 CCCGGG BstAPI 202 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 238 GCAGTTGATGC BstYI 313 GGATCC [GA]^GATC[CT] 772 AGATCC CfoI 379 GCGC GCG^C 393 GCGC Cfr13I 791 GGGCC G^G[ACGT]CC 792 GGCCC FauI 1069 CCCGC CCCGC(4/6) 1199 GCGGG HhaI 379 GCGC GCG^C 393 GCGC HinP1I 379 GCGC G^CGC 393 GCGC HphI 24 GGTGA GGTGA(8/7) 358 GGTGA MflI 313 GGATCC [GA]^GATC[CT] 772 AGATCC NciI 788 CCCGG CC^[GC]GG 789 CCGGG NcoI 157 CCATGG C^CATGG 1073 CCATGG NlaIII 158 CATG CATG^ 1074 CATG RsaI 32 GTAC GT^AC 1065 GTAC Sau96I 791 GGGCC G^G[ACGT]CC 792 GGCCC SmlI 835 CTTGAG C^T[CT][GA]AG 1033 CTTGAG TaqI 83 TCGA T^CGA 97 TCGA TthHB8I 83 TCGA T^CGA 97 TCGA XhoII 313 GGATCC [GA]^GATC[CT] 772 AGATCC XmnI 112 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 952 GAAGTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI AspHI BanII BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I Cac8I DdeI DpnII DsaI EcoT14I Fnu4HI FokI HaeIII ItaI MaeI MboI MboII MnlI MseI MwoI NdeII NlaIV Sau3AI ScrFI SfaNI SfcI StyI Tru9I TseI Tsp509I TspRI