Restriction map for
pFN21AE4425
(Circular) (Six-base) MAPSORT of: pFN21AE4425.seq Check: 5305 from: 1 to: 6025
pFN21AE4425 6025 bp
With 182 enzymes: SgfI *
March 28, 2012 14:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3368 1937
Size: 1431 4594
Acc65I G'GTAC_C
Cuts at: 3069 3346 3069
Size: 277 5748
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2601 2987 3115 4088 5472 5740 1632
Size: 969 386 128 973 1384 268 1917
Fragments arranged by size:
1917 1384 973 969 386 268 128
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 2873 4924 5901 1203
Size: 1670 2051 977 1327
Fragments arranged by size:
2051 1670 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 2788 4754 2788
Size: 1966 4059
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AlwNI CAG_nnn'CTG
Cuts at: 2213 2686 3020 3768 5340 2213
Size: 473 334 748 1572 2898
Fragments arranged by size:
2898 1572 748 473 334
ApaI G_GGCC'C
Cuts at: 2800 2800
Size: 6025
ApaLI G'TGCA_C
Cuts at: 4082 5238 4082
Size: 1156 4869
ApoI r'AATT_y
Cuts at: 1493 2104 2150 2944 3107 3334 3560 3712 5821
Size: 611 46 794 163 227 226 152 2109
Cuts at: 5821 1493
Size: 1697
Fragments arranged by size:
2109 1697 794 611 227 226 163 152
46
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2420 2793 3350 1870
Size: 73 477 373 557 4545
Fragments arranged by size:
4545 557 477 373 73
AvrII C'CTAG_G
Cuts at: 2269 2269
Size: 6025
BamHI G'GATC_C
Cuts at: 2318 3355 3722 2318
Size: 1037 367 4621
Fragments arranged by size:
4621 1037 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3069 3190 3346 4801 618
Size: 325 217 627 1282 121 156 1455 1842
Fragments arranged by size:
1842 1455 1282 627 325 217 156 121
BanII G_rGCy'C
Cuts at: 1391 2800 3028 3168 3344 1391
Size: 1409 228 140 176 4072
Fragments arranged by size:
4072 1409 228 176 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2843 2877 4216 4250 1903
Size: 34 906 34 1339 34 3678
Fragments arranged by size:
3678 1339 906 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2432 3917 5127 2432
Size: 1485 1210 3330
Fragments arranged by size:
3330 1485 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4636 136
Size: 107 122 71 631 605 2964 1525
Fragments arranged by size:
2964 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 2800 4086 5242 1790
Size: 1010 1286 1156 2573
Fragments arranged by size:
2573 1286 1156 1010
BmgBI CAC'GTC
Cuts at: 1318 2874 1318
Size: 1556 4469
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3022 3756 4714
Size: 416 53 83 399 24 1605 734 958
Cuts at: 4714 442
Size: 1753
Fragments arranged by size:
1753 1605 958 734 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2949 3042 4685 1239
Size: 1710 93 1643 2579
Fragments arranged by size:
2579 1710 1643 93
Bpu10I CC'TnA_GC
Cuts at: 2832 2832
Size: 6025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2861 3059 4150 5015 5313 2861
Size: 198 1091 865 298 3573
Fragments arranged by size:
3573 1091 865 298 198
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3035 4688 882
Size: 617 1536 1653 2219
Fragments arranged by size:
2219 1653 1536 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 2259 6019 2259
Size: 3760 2265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3832 4214 4849 275
Size: 53 83 186 936 2299 382 635 1451
Fragments arranged by size:
2299 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4458 4856 5130 5277 5688 4458
Size: 398 274 147 411 4795
Fragments arranged by size:
4795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5800
Fragments arranged by size:
5800 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2894 1186
Size: 1708 4317
BseYI C'CCAG_C
Cuts at: 5228 5724 5228
Size: 496 5529
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2262 1163
Size: 573 526 4926
Fragments arranged by size:
4926 573 526
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2395 2886 3168 4086 4171 5242 2022
Size: 373 491 282 918 85 1071 2805
Fragments arranged by size:
2805 1071 918 491 373 282 85
BsmI GAATG_Cn'
Cuts at: 3536 3629 3536
Size: 93 5932
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2395 2800 2886 3028 3168 3344
Size: 399 232 373 405 86 142 140 176
Cuts at: 3344 4086 4171 5242 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 373 232 176
142 140 86 85
BspEI T'CCGG_A
Cuts at: 5688 5688
Size: 6025
BspHI T'CATG_A
Cuts at: 3914 5644 3914
Size: 1730 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3380 844
Size: 2536 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2433 3912 1394
Size: 429 610 1479 3507
Fragments arranged by size:
3507 1479 610 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2859 4520 4694 65
Size: 1183 1611 1661 174 1396
Fragments arranged by size:
1661 1611 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4669 4856 1700
Size: 110 2859 187 2869
Fragments arranged by size:
2869 2859 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6025
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 2405 4085 5097 2405
Size: 1680 1012 3333
Fragments arranged by size:
3333 1680 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2213 2249 794
Size: 1419 36 4570
Fragments arranged by size:
4570 1419 36
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 2318 2777 3355 3722 4107 4124 5576 5662
Size: 346 459 578 367 385 17 1452 86
Cuts at: 5662 5674 6020 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 578 459 385 367 346 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2162 3078 3186 3264 513
Size: 552 939 158 916 108 78 3274
Fragments arranged by size:
3274 939 916 552 158 108 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 2058 2578 3541 4335 4355 2058
Size: 520 963 794 20 3728
Fragments arranged by size:
3728 963 794 520 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3081 3472 4361
Size: 54 1425 50 275 18 1251 391 889
Cuts at: 4361 5990 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1251 889 391 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3955 1353
Size: 2602 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2785 4612 4986 5132 387
Size: 1064 138 1196 1827 374 146 1280
Fragments arranged by size:
1827 1280 1196 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3166 3166
Size: 6025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2601 2949 2987 3042 3115 4088 4685
Size: 393 969 348 38 55 73 973 597
Cuts at: 4685 5472 5740 1239
Size: 787 268 1524
Fragments arranged by size:
1524 973 969 787 597 393 348 268
73 55 38
EcoNI CCTnn'n_nnAGG
Cuts at: 3198 3198
Size: 6025
EcoO109I rG'GnC_Cy
Cuts at: 2797 2797
Size: 6025
EcoRI G'AATT_C
Cuts at: 2150 3334 3712 2150
Size: 1184 378 4463
Fragments arranged by size:
4463 1184 378
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 5172 1678
Size: 3494 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2769 2774 2801 2806 4666
Size: 32 135 32 1197 5 27 5 1860
Cuts at: 4666 4698 4740 4772 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1860 1197 135 42 32 32 32
32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3615 669
Size: 988 281 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6025
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KpnI G_GTAC'C
Cuts at: 3073 3350 3073
Size: 277 5748
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2346 2805 5139 5323 1305
Size: 1041 459 2334 184 2007
Fragments arranged by size:
2334 2007 1041 459 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4203
Fragments arranged by size:
4203 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2194 3983 4342 4501 518
Size: 648 1028 1789 359 159 2042
Fragments arranged by size:
2042 1789 1028 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2660 3441 4118 5266 5511 1606
Size: 1054 781 677 1148 245 2120
Fragments arranged by size:
2120 1148 1054 781 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2162 3078 513
Size: 552 939 158 916 3460
Fragments arranged by size:
3460 939 916 552 158
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 3383 4928 1132
Size: 97 2154 1545 2229
Fragments arranged by size:
2229 2154 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6025
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2168 1108
Size: 1060 4965
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PspOMI G'GGCC_C
Cuts at: 2796 2796
Size: 6025
PstI C_TGCA'G
Cuts at: 830 2938 3377 830
Size: 2108 439 3478
Fragments arranged by size:
3478 2108 439
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 2660 2660
Size: 6025
SacI G_AGCT'C
Cuts at: 3168 3168
Size: 6025
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SfcI C'TryA_G
Cuts at: 826 1045 2670 2934 3195 3373 4508 5189 5380
Size: 219 1625 264 261 178 1135 681 191
Cuts at: 5380 826
Size: 1471
Fragments arranged by size:
1625 1471 1135 681 264 261 219 191
178
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 2795 3352 1872
Size: 923 557 4545
Fragments arranged by size:
4545 923 557
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2840 3038 4129 5030 5292 820
Size: 197 926 897 198 1091 901 262 1553
Fragments arranged by size:
1553 1091 926 901 897 262 198 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 3383 1132
Size: 2251 3774
SspI AAT'ATT
Cuts at: 5 52 3949 5
Size: 47 3897 2081
Fragments arranged by size:
3897 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6025
StyI C'CwwG_G
Cuts at: 513 1065 2004 2162 2269 2347 2567 3078 3236
Size: 552 939 158 107 78 220 511 158
Cuts at: 3236 3464 513
Size: 228 3074
Fragments arranged by size:
3074 939 552 511 228 220 158 158
107 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2012 4052 4069 4222 4407 4852 325
Size: 1255 432 2040 17 153 185 445 1498
Fragments arranged by size:
2040 1498 1255 445 432 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4271 5983 96
Size: 274 80 33 51 1443 2294 1712 138
Fragments arranged by size:
2294 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5985 6017 5985
Size: 32 5993
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6025
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6025
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 2793 3350 1870
Size: 923 557 4545
Fragments arranged by size:
4545 923 557
XmnI GAAnn'nnTTC
Cuts at: 2121 2961 4154 2121
Size: 840 1193 3992
Fragments arranged by size:
3992 1193 840
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI