(Linear) (Six-base) MAPSORT of: KIEE4425.seq Check: 5272 from: 1 to: 1320 KIEE4425.orf With 182 enzymes: SgfI * March 22, 2012 17:59 .. Acc65I G'GTAC_C Cuts at: 0 1064 1320 Size: 1064 256 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 596 982 1110 1320 Size: 596 386 128 210 Fragments arranged by size: 596 386 210 128 AflIII A'CryG_T Cuts at: 0 868 1320 Size: 868 452 AhdI GACnn_n'nnGTC Cuts at: 0 783 1320 Size: 783 537 AlwNI CAG_nnn'CTG Cuts at: 0 208 681 1015 1320 Size: 208 473 334 305 Fragments arranged by size: 473 334 305 208 ApaI G_GGCC'C Cuts at: 0 795 1320 Size: 795 525 ApoI r'AATT_y Cuts at: 0 99 145 939 1102 1320 Size: 99 46 794 163 218 Fragments arranged by size: 794 218 163 99 46 AvaI C'yCGr_G Cuts at: 0 415 788 1320 Size: 415 373 532 Fragments arranged by size: 532 415 373 AvrII C'CTAG_G Cuts at: 0 264 1320 Size: 264 1056 BamHI G'GATC_C Cuts at: 0 313 1320 Size: 313 1007 BanI G'GyrC_C Cuts at: 0 1064 1185 1320 Size: 1064 121 135 Fragments arranged by size: 1064 135 121 BanII G_rGCy'C Cuts at: 0 795 1023 1163 1320 Size: 795 228 140 157 Fragments arranged by size: 795 228 157 140 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 838 872 1320 Size: 838 34 448 Fragments arranged by size: 838 448 34 BciVI GTATCCnnnnn_n' Cuts at: 0 427 1320 Size: 427 893 Bme1580I G_kGCm'C Cuts at: 0 795 1320 Size: 795 525 BmgBI CAC'GTC Cuts at: 0 869 1320 Size: 869 451 BmrI ACTGGGnnnn_n' Cuts at: 0 1017 1320 Size: 1017 303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 944 1037 1320 Size: 944 93 283 Fragments arranged by size: 944 283 93 Bpu10I CC'TnA_GC Cuts at: 0 827 1320 Size: 827 493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 856 1054 1320 Size: 856 198 266 Fragments arranged by size: 856 266 198 BsaI GGTCTCn'nnnn_ Cuts at: 0 1030 1320 Size: 1030 290 BsaBI GATnn'nnATC Cuts at: 0 254 1320 Size: 254 1066 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 889 1320 Size: 889 431 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 257 1320 Size: 257 1063 BsiHKAI G_wGCw'C Cuts at: 0 17 390 881 1163 1320 Size: 17 373 491 282 157 Fragments arranged by size: 491 373 282 157 17 Bsp1286I G_dGCh'C Cuts at: 0 17 390 795 881 1023 1163 1320 Size: 17 373 405 86 142 140 157 Fragments arranged by size: 405 373 157 142 140 86 17 BsrBI CCG'CTC Cuts at: 0 428 1320 Size: 428 892 BsrDI GCAATG_nn' Cuts at: 0 854 1320 Size: 854 466 BssSI C'ACGA_G Cuts at: 0 400 1320 Size: 400 920 BstAPI GCAn_nnn'nTGC Cuts at: 0 208 244 1320 Size: 208 36 1076 Fragments arranged by size: 1076 208 36 BstYI r'GATC_y Cuts at: 0 313 772 1320 Size: 313 459 548 Fragments arranged by size: 548 459 313 BtgI C'CryG_G Cuts at: 0 157 1073 1181 1259 1320 Size: 157 916 108 78 61 Fragments arranged by size: 916 157 108 78 61 BtsI GCAGTG_nn' Cuts at: 0 53 573 1320 Size: 53 520 747 Fragments arranged by size: 747 520 53 EaeI y'GGCC_r Cuts at: 0 1076 1320 Size: 1076 244 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 780 1320 Size: 780 540 EcoICRI GAG'CTC Cuts at: 0 1161 1320 Size: 1161 159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 596 944 982 1037 1110 1320 Size: 596 348 38 55 73 210 Fragments arranged by size: 596 348 210 73 55 38 EcoNI CCTnn'n_nnAGG Cuts at: 0 1193 1320 Size: 1193 127 EcoO109I rG'GnC_Cy Cuts at: 0 792 1320 Size: 792 528 EcoRI G'AATT_C Cuts at: 0 145 1320 Size: 145 1175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 764 769 796 801 1320 Size: 764 5 27 5 519 Fragments arranged by size: 764 519 27 5 5 KpnI G_GTAC'C Cuts at: 0 1068 1320 Size: 1068 252 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 341 800 1320 Size: 341 459 520 Fragments arranged by size: 520 459 341 MslI CAynn'nnrTG Cuts at: 0 189 1320 Size: 189 1131 MspA1I CmG'CkG Cuts at: 0 655 1320 Size: 655 665 NcoI C'CATG_G Cuts at: 0 157 1073 1320 Size: 157 916 247 Fragments arranged by size: 916 247 157 PflMI CCAn_nnn'nTGG Cuts at: 0 163 1320 Size: 163 1157 PspOMI G'GGCC_C Cuts at: 0 791 1320 Size: 791 529 PstI C_TGCA'G Cuts at: 0 933 1320 Size: 933 387 PvuII CAG'CTG Cuts at: 0 655 1320 Size: 655 665 SacI G_AGCT'C Cuts at: 0 1163 1320 Size: 1163 157 SfcI C'TryA_G Cuts at: 0 665 929 1190 1320 Size: 665 264 261 130 Fragments arranged by size: 665 264 261 130 SmaI CCC'GGG Cuts at: 0 790 1320 Size: 790 530 SmlI C'TyrA_G Cuts at: 0 835 1033 1320 Size: 835 198 287 Fragments arranged by size: 835 287 198 StyI C'CwwG_G Cuts at: 0 157 264 342 562 1073 1231 1320 Size: 157 107 78 220 511 158 89 Fragments arranged by size: 511 220 158 157 107 89 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 7 1320 Size: 7 1313 XmaI C'CCGG_G Cuts at: 0 788 1320 Size: 788 532 XmnI GAAnn'nnTTC Cuts at: 0 116 956 1320 Size: 116 840 364 Fragments arranged by size: 840 364 116 Enzymes that do cut: Acc65I AcuI AflIII AhdI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BcgI BciVI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BseRI BsgI BsiHKAI Bsp1286I BsrBI BsrDI BssSI BstAPI BstYI BtgI BtsI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I KpnI MmeI MslI MspA1I NcoI PflMI PspOMI PstI PvuII SacI SfcI SmaI SmlI StyI TaqII XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI BbeI BbsI BbvCI BclI BfrBI BglI BglII BlpI BmtI BplI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI