Restriction map for
pFN21AE1039
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BfrI BglI
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FseI
FspI HaeII HhaI Hin1I HinP1I
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 676 CGCG CG^CG
AseI 646 ATTAAT AT^TAAT
AsnI 646 ATTAAT AT^TAAT
Asp700I 895 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 984 GGACC G^G[AT]CC
BanI 830 GGCACC G^G[CT][GA]CC
BanII 489 GAGCCC G[GA]GC[CT]^C
BciVI 1078 GGATAC GTATCC(6/5)
BclI 410 TGATCA T^GATCA
BcnI 31 CCGGG CC^[GC]GG
BglII 1344 AGATCT A^GATCT
BlpI 553 GCTAAGC GC^T[ACGT]AGC
BmyI 489 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 553 GCTAAGC GC^T[ACGT]AGC
BsmFI 1167 GGGAC GGGAC(10/14)
Bsp1286I 489 GAGCCC G[AGT]GC[ACT]^C
BspHI 44 TCATGA T^CATGA
BspLU11I 848 ACATGT A^CATGT
BspMI 389 GCAGGT ACCTGC(4/8)
BstUI 676 CGCG CG^CG
Cac8I 487 GCGAGC GC[ACGT]^[ACGT]GC
CelII 553 GCTAAGC GC^T[ACGT]AGC
EaeI 11 TGGCCG [CT]^GGCC[GA]
EcoT22I 1161 ATGCAT ATGCA^T
FauI 620 GCGGG CCCGC(4/6)
FbaI 410 TGATCA T^GATCA
HincII 625 GTTAAC GT[CT]^[GA]AC
HindII 625 GTTAAC GT[CT]^[GA]AC
HindIII 249 AAGCTT A^AGCTT
HpaI 625 GTTAAC GTT^AAC
HphI 295 TCACC GGTGA(8/7)
MaeII 516 ACGT A^CGT
MvnI 676 CGCG CG^CG
NciI 31 CCGGG CC^[GC]GG
NsiI 1161 ATGCAT ATGCA^T
NspI 848 ACATGT [GA]CATG^[CT]
PshBI 646 ATTAAT AT^TAAT
PstI 277 CTGCAG CTGCA^G
RcaI 44 TCATGA T^CATGA
XmnI 895 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 844 GTAC GT^AC
852 GTAC
AflIII 514 ACACGT A^C[GA][CT]GT
848 ACATGT
AlwNI 887 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1051 CAGCTTCTG
BbsI 48 GAAGAC GAAGAC(2/6)
924 GAAGAC
BpmI 363 CTCCAG CTGGAG(16/14)
536 CTCCAG
BpuAI 48 GAAGAC GAAGAC(2/6)
924 GAAGAC
BsaJI 8 CCCTGG C^C[ACGT][ACGT]GG
1367 CCTGGG
BsiYI 14 CCGAGTCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
613 CCTGTTTGCGG
BslI 14 CCGAGTCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
613 CCTGTTTGCGG
BsmAI 511 GAGAC GTCTC(1/5)
18 GTCTC
Bsp1407I 843 TGTACA T^GTACA
851 TGTACA
BsrDI 99 CATTGC GCAATG(2/0)
103 GCAATG
BsrGI 843 TGTACA T^GTACA
851 TGTACA
BsrI 240 ACTGG ACTGG(1/-1)
1325 CCAGT
Cfr13I 33 GGGCC G^G[ACGT]CC
984 GGACC
DraI 375 TTTAAA TTT^AAA
765 TTTAAA
HapII 31 CCGG C^CGG
208 CCGG
HgaI 558 GCGTC GACGC(5/10)
936 GCGTC
HpaII 31 CCGG C^CGG
208 CCGG
MslI 99 CATTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1036 CATAAGAGTG
MspI 31 CCGG C^CGG
208 CCGG
NlaIV 830 GGCACC GG[ACGT]^[ACGT]CC
1239 GGAACC
PvuII 663 CAGCTG CAG^CTG
887 CAGCTG
RsaI 844 GTAC GT^AC
852 GTAC
Sau96I 33 GGGCC G^G[ACGT]CC
984 GGACC
SspBI 843 TGTACA T^GTACA
851 TGTACA
TspRI 118 CAGTG CA[GC]TG[ACGT][ACGT]^
175 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BfaI BssKI BstF5I BstNI
BstYI DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII PleI Sau3AI
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TthHB8I
XhoII