(Circular) (Six-base) MAPSORT of: pFN21AE1039.seq Check: 666 from: 1 to: 6190 pFN21AE1039 6190 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3533 1937 Size: 1596 4594 Acc65I G'GTAC_C Cuts at: 3511 3511 Size: 6190 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4253 5637 5905 1632 Size: 2621 1384 268 1917 Fragments arranged by size: 2621 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5993 AflIII A'CryG_T Cuts at: 1203 2519 2853 5089 6066 1203 Size: 1316 334 2236 977 1327 Fragments arranged by size: 2236 1327 1316 977 334 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 4919 4919 Size: 6190 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6190 AlwNI CAG_nnn'CTG Cuts at: 2897 3061 3933 5505 2897 Size: 164 872 1572 3582 Fragments arranged by size: 3582 1572 872 164 ApaLI G'TGCA_C Cuts at: 4247 5403 4247 Size: 1156 5034 ApoI r'AATT_y Cuts at: 1493 2082 2159 2260 2471 2843 3050 3499 3725 Size: 589 77 101 211 372 207 449 226 Cuts at: 3725 3877 5986 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 589 449 372 226 211 207 152 101 77 AseI AT'TA_AT Cuts at: 160 1032 2652 4744 160 Size: 872 1620 2092 1606 Fragments arranged by size: 2092 1620 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 3515 1870 Size: 73 1572 4545 Fragments arranged by size: 4545 1572 73 BamHI G'GATC_C Cuts at: 3520 3887 3520 Size: 367 5823 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2835 3511 4966 618 Size: 325 217 627 1048 676 1455 1842 Fragments arranged by size: 1842 1455 1048 676 627 325 217 BanII G_rGCy'C Cuts at: 1391 2498 3509 1391 Size: 1107 1011 4072 Fragments arranged by size: 4072 1107 1011 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2060 2936 928 Size: 971 161 876 4182 Fragments arranged by size: 4182 971 876 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4381 4415 1903 Size: 34 2444 34 3678 Fragments arranged by size: 3678 2444 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3077 4082 5292 3077 Size: 1005 1210 3975 Fragments arranged by size: 3975 1210 1005 BclI T'GATC_A Cuts at: 1548 2415 1548 Size: 867 5323 BfrBI ATG'CAT Cuts at: 3168 3168 Size: 6190 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4801 136 Size: 107 122 71 631 605 3129 1525 Fragments arranged by size: 3129 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3349 6185 3349 Size: 2836 3354 BlpI GC'TnA_GC Cuts at: 2559 3607 2559 Size: 1048 5142 Bme1580I G_kGCm'C Cuts at: 1790 4251 5407 1790 Size: 2461 1156 2573 Fragments arranged by size: 2573 2461 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3921 4879 442 Size: 416 53 83 399 24 2504 958 1753 Fragments arranged by size: 2504 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2593 2593 Size: 6190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2353 2526 4850 1239 Size: 1114 173 2324 2579 Fragments arranged by size: 2579 2324 1114 173 Bpu10I CC'TnA_GC Cuts at: 2568 2568 Size: 6190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4315 5180 5478 4315 Size: 865 298 5027 Fragments arranged by size: 5027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4853 882 Size: 617 3354 2219 Fragments arranged by size: 3354 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3981 493 Size: 711 2777 2702 Fragments arranged by size: 2777 2702 711 BsaBI GATnn'nnATC Cuts at: 6184 6184 Size: 6190 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3997 4379 5014 275 Size: 53 83 186 936 2464 382 635 1451 Fragments arranged by size: 2464 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4623 5021 5295 5442 5853 4623 Size: 398 274 147 411 4960 Fragments arranged by size: 4960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5965 Fragments arranged by size: 5965 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6190 BseYI C'CCAG_C Cuts at: 5393 5889 5393 Size: 496 5694 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5617 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3640 4401 5429 1540 Size: 275 186 1639 761 1028 2301 Fragments arranged by size: 2301 1639 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4251 4336 5407 4251 Size: 85 1071 5034 Fragments arranged by size: 5034 1071 85 BsmI GAATG_Cn' Cuts at: 3701 3794 3701 Size: 93 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2498 3509 4251 4336 5407 1391 Size: 399 708 1011 742 85 1071 2174 Fragments arranged by size: 2174 1071 1011 742 708 399 85 BspEI T'CCGG_A Cuts at: 5853 5853 Size: 6190 BspHI T'CATG_A Cuts at: 2049 4079 5809 2049 Size: 2030 1730 2430 Fragments arranged by size: 2430 2030 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2385 3545 844 Size: 1541 1160 3489 Fragments arranged by size: 3489 1541 1160 BsrBI CCG'CTC Cuts at: 1394 1823 4077 1394 Size: 429 2254 3507 Fragments arranged by size: 3507 2254 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2103 2115 4685 4859 65 Size: 1183 855 12 2570 174 1396 Fragments arranged by size: 2570 1396 1183 855 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 4834 5021 1700 Size: 110 3024 187 2869 Fragments arranged by size: 3024 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2848 2856 96 Size: 2752 8 3430 Fragments arranged by size: 3430 2752 8 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6190 BssSI C'ACGA_G Cuts at: 4250 5262 4250 Size: 1012 5178 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6190 BstBI TT'CG_AA Cuts at: 4994 4994 Size: 6190 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6190 BstYI r'GATC_y Cuts at: 1972 2215 2545 3349 3520 3887 4272 4289 5741 Size: 243 330 804 171 367 385 17 1452 Cuts at: 5741 5827 5839 6185 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 804 385 367 346 330 243 171 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4699 Fragments arranged by size: 4699 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2886 487 Size: 683 56 1660 3791 Fragments arranged by size: 3791 1660 683 56 BtsI GCAGTG_nn' Cuts at: 3706 4500 4520 3706 Size: 794 20 5376 Fragments arranged by size: 5376 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6155 DraI TTT'AAA Cuts at: 2382 2772 3494 2382 Size: 390 722 5078 Fragments arranged by size: 5078 722 390 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 3637 4526 Size: 54 1425 50 275 18 186 1621 889 Cuts at: 4526 6155 8 Size: 1629 43 Fragments arranged by size: 1629 1621 1425 889 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3637 1537 Size: 275 1825 4090 Fragments arranged by size: 4090 1825 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2332 2526 2679 3252 3357 4120 1353 Size: 979 194 153 573 105 763 3423 Fragments arranged by size: 3423 979 763 573 194 153 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4777 5151 5297 387 Size: 1064 138 3188 374 146 1280 Fragments arranged by size: 3188 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2353 2526 4253 4850 5637 5905 1239 Size: 393 721 173 1727 597 787 268 1524 Fragments arranged by size: 1727 1524 787 721 597 393 268 173 EcoRI G'AATT_C Cuts at: 3499 3877 3499 Size: 378 5812 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3311 3311 Size: 6190 FspI TGC'GCA Cuts at: 4696 4696 Size: 6190 HaeII r_GCGC'y Cuts at: 1678 5337 1678 Size: 3659 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2104 2136 4831 4863 4905 Size: 32 135 32 532 32 2695 32 42 Cuts at: 4905 4937 1373 Size: 32 2626 Fragments arranged by size: 2695 2626 532 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2632 3534 3780 669 Size: 988 281 694 902 246 3079 Fragments arranged by size: 3079 988 902 694 281 246 HindIII A'AGCT_T Cuts at: 748 2254 748 Size: 1506 4684 HpaI GTT'AAC Cuts at: 2632 3780 2632 Size: 1148 5042 KpnI G_GTAC'C Cuts at: 3515 3515 Size: 6190 MfeI C'AATT_G Cuts at: 3789 3789 Size: 6190 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2210 5304 5488 1305 Size: 905 3094 184 2007 Fragments arranged by size: 3094 2007 905 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4368 Fragments arranged by size: 4368 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 3045 4148 4507 4666 518 Size: 648 942 937 1103 359 159 2042 Fragments arranged by size: 2042 1103 942 937 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2670 2731 2894 3446 3606 4283 5431 5676 Size: 1064 61 163 552 160 677 1148 245 Cuts at: 5676 1606 Size: 2120 Fragments arranged by size: 2120 1148 1064 677 552 245 163 160 61 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4699 Fragments arranged by size: 4699 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6190 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 3637 3637 Size: 6190 NsiI A_TGCA'T Cuts at: 3170 3170 Size: 6190 NspI r_CATG'y Cuts at: 1132 1229 2857 3548 5093 1132 Size: 97 1628 691 1545 2229 Fragments arranged by size: 2229 1628 1545 691 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6190 PciI A'CATG_T Cuts at: 2853 5089 2853 Size: 2236 3954 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6190 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6190 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PstI C_TGCA'G Cuts at: 830 2286 3542 830 Size: 1456 1256 3478 Fragments arranged by size: 3478 1456 1256 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 PvuII CAG'CTG Cuts at: 2670 2894 2670 Size: 224 5966 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SfcI C'TryA_G Cuts at: 826 1045 2282 2361 2526 3144 3538 4673 5354 Size: 219 1237 79 165 618 394 1135 681 Cuts at: 5354 5545 826 Size: 191 1471 Fragments arranged by size: 1471 1237 1135 681 618 394 219 191 165 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 3517 1872 Size: 1645 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4294 5195 5457 820 Size: 197 926 2351 901 262 1553 Fragments arranged by size: 2351 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 3548 1132 Size: 2416 3774 SspI AAT'ATT Cuts at: 5 52 4114 5 Size: 47 4062 2081 Fragments arranged by size: 4062 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6190 StyI C'CwwG_G Cuts at: 513 1065 2004 3629 513 Size: 552 939 1625 3074 Fragments arranged by size: 3074 1625 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4217 4234 4387 4572 5017 325 Size: 1255 2637 17 153 185 445 1498 Fragments arranged by size: 2637 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2848 2856 4436 Size: 274 80 33 51 1443 871 8 1580 Cuts at: 4436 6148 96 Size: 1712 138 Fragments arranged by size: 1712 1580 1443 871 274 138 80 51 33 8 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6150 6182 6150 Size: 32 6158 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6190 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6190 XmaI C'CCGG_G Cuts at: 1870 3515 1870 Size: 1645 4545 XmnI GAAnn'nnTTC Cuts at: 2904 4319 2904 Size: 1415 4775 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI