(Circular) (Six-base) MAPSORT of: pFN21AE1526.seq Check: 8396 from: 1 to: 5791 pFN21AE1526 5791 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2463 2548 2728 2463 Size: 85 180 5526 Fragments arranged by size: 5526 180 85 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3601 278 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3134 1937 Size: 1197 4594 Acc65I G'GTAC_C Cuts at: 2469 3112 2469 Size: 643 5148 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2058 2205 3854 5238 5506 1632 Size: 426 147 1649 1384 268 1917 Fragments arranged by size: 1917 1649 1384 426 268 147 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 4690 5667 1203 Size: 3487 977 1327 Fragments arranged by size: 3487 1327 977 AgeI A'CCGG_T Cuts at: 4622 4622 Size: 5791 AhdI GACnn_n'nnGTC Cuts at: 4520 4520 Size: 5791 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5791 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2449 2471 1184 Size: 1265 22 4504 Fragments arranged by size: 4504 1265 22 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3014 3046 3014 Size: 32 5759 AlwNI CAG_nnn'CTG Cuts at: 2236 2563 2782 2842 2995 3534 5106 2236 Size: 327 219 60 153 539 1572 2921 Fragments arranged by size: 2921 1572 539 327 219 153 60 ApaI G_GGCC'C Cuts at: 2383 2383 Size: 5791 ApaLI G'TGCA_C Cuts at: 3848 5004 3848 Size: 1156 4635 ApoI r'AATT_y Cuts at: 1493 3100 3326 3478 5587 1493 Size: 1607 226 152 2109 1697 Fragments arranged by size: 2109 1697 1607 226 152 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 2119 3116 1870 Size: 73 176 997 4545 Fragments arranged by size: 4545 997 176 73 BamHI G'GATC_C Cuts at: 3121 3488 3121 Size: 367 5424 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2469 2573 3112 4567 618 Size: 325 217 627 682 104 539 1455 1842 Fragments arranged by size: 1842 1455 682 627 539 325 217 104 BanII G_rGCy'C Cuts at: 1391 2120 2183 2383 2899 2973 3110 1391 Size: 729 63 200 516 74 137 4072 Fragments arranged by size: 4072 729 516 200 137 74 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3982 4016 1903 Size: 34 2045 34 3678 Fragments arranged by size: 3678 2045 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3683 4893 3683 Size: 1210 4581 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2252 2351 2703 Size: 107 122 71 631 605 580 99 352 Cuts at: 2703 4402 136 Size: 1699 1525 Fragments arranged by size: 1699 1525 631 605 580 352 122 107 99 71 BglII A'GATC_T Cuts at: 5786 5786 Size: 5791 BlpI GC'TnA_GC Cuts at: 3208 3208 Size: 5791 Bme1580I G_kGCm'C Cuts at: 1790 2383 2578 3852 5008 1790 Size: 593 195 1274 1156 2573 Fragments arranged by size: 2573 1274 1156 593 195 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5791 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2966 3522 4480 Size: 416 53 83 399 24 1549 556 958 Cuts at: 4480 442 Size: 1753 Fragments arranged by size: 1753 1549 958 556 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2187 2405 2495 2984 3046 3047 4451 1239 Size: 948 218 90 489 62 1 1404 2579 Fragments arranged by size: 2579 1404 948 489 218 90 62 1 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2005 2735 3916 4781 5079 2005 Size: 730 1181 865 298 2717 Fragments arranged by size: 2717 1181 865 730 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4454 882 Size: 617 2955 2219 Fragments arranged by size: 2955 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2526 2887 3582 493 Size: 711 1322 361 695 2702 Fragments arranged by size: 2702 1322 711 695 361 BsaBI GATnn'nnATC Cuts at: 2168 5785 2168 Size: 3617 2174 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2227 2610 3598 3980 Size: 53 83 186 936 694 383 988 382 Cuts at: 3980 4615 275 Size: 635 1451 Fragments arranged by size: 1451 988 936 694 635 383 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4224 4622 4896 5043 5454 4224 Size: 398 274 147 411 4561 Fragments arranged by size: 4561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2130 2160 2234 2264 3014 Size: 30 165 30 727 30 74 30 750 Cuts at: 3014 3044 1178 Size: 30 3925 Fragments arranged by size: 3925 750 727 165 74 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2095 2190 2234 2291 2963 2981 3023 3035 Size: 909 95 44 57 672 18 42 12 Cuts at: 3035 1186 Size: 3942 Fragments arranged by size: 3942 909 672 95 57 44 42 18 12 BseYI C'CCAG_C Cuts at: 2111 2336 2563 2675 3067 4994 5490 2111 Size: 225 227 112 392 1927 496 2412 Fragments arranged by size: 2412 1927 496 392 227 225 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2548 2762 2923 1163 Size: 573 812 214 161 4031 Fragments arranged by size: 4031 812 573 214 161 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2358 3241 4002 5030 1540 Size: 275 186 357 883 761 1028 2301 Fragments arranged by size: 2301 1028 883 761 357 275 186 BsiHKAI G_wGCw'C Cuts at: 2822 2868 2886 2973 3852 3937 5008 2822 Size: 46 18 87 879 85 1071 3605 Fragments arranged by size: 3605 1071 879 87 85 46 18 BsmI GAATG_Cn' Cuts at: 2765 3302 3395 2765 Size: 537 93 5161 Fragments arranged by size: 5161 537 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2183 2383 2578 2822 2868 2886 Size: 399 330 63 200 195 244 46 18 Cuts at: 2886 2899 2973 3110 3852 3937 5008 1391 Size: 13 74 137 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 330 244 200 195 137 85 74 63 46 18 13 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 3680 5410 3680 Size: 1730 4061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2463 2548 2728 2790 3146 844 Size: 1619 85 180 62 356 3489 Fragments arranged by size: 3489 1619 356 180 85 62 BsrBI CCG'CTC Cuts at: 1394 1823 3678 1394 Size: 429 1855 3507 Fragments arranged by size: 3507 1855 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4286 4460 65 Size: 1183 3038 174 1396 Fragments arranged by size: 3038 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2466 4435 4622 1700 Size: 110 656 1969 187 2869 Fragments arranged by size: 2869 1969 656 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5791 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5791 BssSI C'ACGA_G Cuts at: 2879 3851 4863 2879 Size: 972 1012 3807 Fragments arranged by size: 3807 1012 972 BstAPI GCAn_nnn'nTGC Cuts at: 794 2740 2782 2932 794 Size: 1946 42 150 3653 Fragments arranged by size: 3653 1946 150 42 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5791 BstXI CCAn_nnnn'nTGG Cuts at: 2444 2830 2444 Size: 386 5405 BstYI r'GATC_y Cuts at: 1972 3121 3488 3873 3890 5342 5428 5440 5786 Size: 1149 367 385 17 1452 86 12 346 Cuts at: 5786 1972 Size: 1977 Fragments arranged by size: 1977 1452 1149 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2175 2823 513 Size: 552 1110 648 3481 Fragments arranged by size: 3481 1110 648 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5052 Fragments arranged by size: 5052 683 56 BtsI GCAGTG_nn' Cuts at: 2331 3307 4101 4121 2331 Size: 976 794 20 4001 Fragments arranged by size: 4001 976 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5756 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4798 809 Size: 507 3482 1802 Fragments arranged by size: 3482 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2355 2394 Size: 54 1425 50 275 18 336 189 39 Cuts at: 2394 3238 4127 5756 8 Size: 844 889 1629 43 Fragments arranged by size: 1629 1425 889 844 336 275 189 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2355 3238 1537 Size: 275 543 883 4090 Fragments arranged by size: 4090 883 543 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2077 2181 2288 2645 3721 1353 Size: 724 104 107 357 1076 3423 Fragments arranged by size: 3423 1076 724 357 107 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2684 2966 4378 4752 4898 387 Size: 1064 138 1095 282 1412 374 146 1280 Fragments arranged by size: 1412 1280 1095 1064 374 282 146 138 EcoICRI GAG'CTC Cuts at: 2971 2971 Size: 5791 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2187 2205 2405 2495 2984 3046 Size: 393 426 129 18 200 90 489 62 Cuts at: 3046 3047 3854 4451 5238 5506 1239 Size: 1 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 597 489 426 393 268 200 129 90 62 18 1 EcoO109I rG'GnC_Cy Cuts at: 2379 2380 2635 2811 2379 Size: 1 255 176 5359 Fragments arranged by size: 5359 255 176 1 EcoRI G'AATT_C Cuts at: 3100 3478 3100 Size: 378 5413 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2656 2656 Size: 5791 FspI TGC'GCA Cuts at: 4297 4297 Size: 5791 HaeII r_GCGC'y Cuts at: 1678 2048 4938 1678 Size: 370 2890 2531 Fragments arranged by size: 2890 2531 370 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2234 2266 2693 Size: 32 135 32 431 32 199 32 427 Cuts at: 2693 2725 4432 4464 4506 4538 1373 Size: 32 1707 32 42 32 2626 Fragments arranged by size: 2626 1707 431 427 199 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3135 3381 669 Size: 988 281 1197 246 3079 Fragments arranged by size: 3079 1197 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5791 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KpnI G_GTAC'C Cuts at: 2473 3116 2473 Size: 643 5148 MfeI C'AATT_G Cuts at: 3390 3390 Size: 5791 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4905 5089 1305 Size: 3600 184 2007 Fragments arranged by size: 3600 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2168 10 Size: 54 1425 343 336 3633 Fragments arranged by size: 3633 1425 343 336 54 MslI CAynn'nnrTG Cuts at: 518 1166 2828 3749 4108 4267 518 Size: 648 1662 921 359 159 2042 Fragments arranged by size: 2042 1662 921 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2177 2272 2440 2842 2985 2992 3071 3207 Size: 571 95 168 402 143 7 79 136 Cuts at: 3207 3884 5032 5277 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 571 402 245 168 143 136 95 79 7 NcoI C'CATG_G Cuts at: 513 1065 2823 513 Size: 552 1758 3481 Fragments arranged by size: 3481 1758 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 2452 3149 4694 1132 Size: 97 1223 697 1545 2229 Fragments arranged by size: 2229 1545 1223 697 97 PasI CC'CwG_GG Cuts at: 1348 2062 1348 Size: 714 5077 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 2842 1108 Size: 1734 4057 PfoI T'CCnGG_A Cuts at: 2550 2550 Size: 5791 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PmlI CAC'GTG Cuts at: 2526 2887 2526 Size: 361 5430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3014 3046 3860 3892 5373 5405 3014 Size: 32 814 32 1481 32 3400 Fragments arranged by size: 3400 1481 814 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2811 2811 Size: 5791 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PspOMI G'GGCC_C Cuts at: 2379 2379 Size: 5791 PstI C_TGCA'G Cuts at: 830 2163 2241 2688 2778 3000 3143 830 Size: 1333 78 447 90 222 143 3478 Fragments arranged by size: 3478 1333 447 222 143 90 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 PvuII CAG'CTG Cuts at: 2440 2842 2992 2440 Size: 402 150 5239 Fragments arranged by size: 5239 402 150 SacI G_AGCT'C Cuts at: 2973 2973 Size: 5791 SacII CC_GC'GG Cuts at: 2178 2178 Size: 5791 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SanDI GG'GwC_CC Cuts at: 2811 2811 Size: 5791 SapI GCTCTTCn'nnn_ Cuts at: 2077 2181 2288 2077 Size: 104 107 5580 Fragments arranged by size: 5580 107 104 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SfcI C'TryA_G Cuts at: 826 1045 2159 2237 2684 2774 2996 3047 3139 Size: 219 1114 78 447 90 222 51 92 Cuts at: 3139 4274 4955 5146 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1114 681 447 222 219 191 92 90 78 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5791 SmaI CCC'GGG Cuts at: 1872 3118 1872 Size: 1246 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2020 2750 3895 4796 5058 820 Size: 197 926 77 730 1145 901 262 1553 Fragments arranged by size: 1553 1145 926 901 730 262 197 77 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 2452 3149 1132 Size: 1320 697 3774 Fragments arranged by size: 3774 1320 697 SspI AAT'ATT Cuts at: 5 52 3715 5 Size: 47 3663 2081 Fragments arranged by size: 3663 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5791 StyI C'CwwG_G Cuts at: 513 1065 2149 2823 3230 513 Size: 552 1084 674 407 3074 Fragments arranged by size: 3074 1084 674 552 407 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2561 3818 3835 3988 4173 4618 325 Size: 1255 981 1257 17 153 185 445 1498 Fragments arranged by size: 1498 1257 1255 981 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4037 5749 96 Size: 274 80 33 51 1443 2060 1712 138 Fragments arranged by size: 2060 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2130 2162 5751 5783 2130 Size: 32 3589 32 2138 Fragments arranged by size: 3589 2138 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5791 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2653 2653 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 3116 1870 Size: 1246 4545 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3599 276 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI SfoI SrfI SwaI