(Linear) (Six-base) MAPSORT of: KIEE1526.seq Check: 4839 from: 1 to: 1086 KIEE1526.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 458 543 723 1086 Size: 458 85 180 363 Fragments arranged by size: 458 363 180 85 Acc65I G'GTAC_C Cuts at: 0 464 1086 Size: 464 622 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 53 200 1086 Size: 53 147 886 Fragments arranged by size: 886 147 53 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 444 466 1086 Size: 444 22 620 Fragments arranged by size: 620 444 22 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1009 1041 1086 Size: 1009 32 45 Fragments arranged by size: 1009 45 32 AlwNI CAG_nnn'CTG Cuts at: 0 231 558 777 837 990 1086 Size: 231 327 219 60 153 96 Fragments arranged by size: 327 231 219 153 96 60 ApaI G_GGCC'C Cuts at: 0 378 1086 Size: 378 708 AvaI C'yCGr_G Cuts at: 0 114 1086 Size: 114 972 BanI G'GyrC_C Cuts at: 0 464 568 1086 Size: 464 104 518 Fragments arranged by size: 518 464 104 BanII G_rGCy'C Cuts at: 0 115 178 378 894 968 1086 Size: 115 63 200 516 74 118 Fragments arranged by size: 516 200 118 115 74 63 BglI GCCn_nnn'nGGC Cuts at: 0 247 346 698 1086 Size: 247 99 352 388 Fragments arranged by size: 388 352 247 99 Bme1580I G_kGCm'C Cuts at: 0 378 573 1086 Size: 378 195 513 Fragments arranged by size: 513 378 195 BmrI ACTGGGnnnn_n' Cuts at: 0 961 1086 Size: 961 125 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 400 490 979 1041 1042 1086 Size: 182 218 90 489 62 1 44 Fragments arranged by size: 489 218 182 90 62 44 1 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 730 1086 Size: 730 356 BsaAI yAC'GTr Cuts at: 0 521 882 1086 Size: 521 361 204 Fragments arranged by size: 521 361 204 BsaBI GATnn'nnATC Cuts at: 0 163 1086 Size: 163 923 BsaHI Gr'CG_yC Cuts at: 0 222 605 1086 Size: 222 383 481 Fragments arranged by size: 481 383 222 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 125 155 229 259 1009 1039 1086 Size: 125 30 74 30 750 30 47 Fragments arranged by size: 750 125 74 47 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 90 185 229 286 958 976 1018 1030 Size: 90 95 44 57 672 18 42 12 Cuts at: 1030 1086 Size: 56 Fragments arranged by size: 672 95 90 57 56 44 42 18 12 BseYI C'CCAG_C Cuts at: 0 106 331 558 670 1062 1086 Size: 106 225 227 112 392 24 Fragments arranged by size: 392 227 225 112 106 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 543 757 918 1086 Size: 543 214 161 168 Fragments arranged by size: 543 214 168 161 BsiEI CG_ry'CG Cuts at: 0 353 1086 Size: 353 733 BsiHKAI G_wGCw'C Cuts at: 0 817 863 881 968 1086 Size: 817 46 18 87 118 Fragments arranged by size: 817 118 87 46 18 BsmI GAATG_Cn' Cuts at: 0 760 1086 Size: 760 326 Bsp1286I G_dGCh'C Cuts at: 0 115 178 378 573 817 863 881 894 Size: 115 63 200 195 244 46 18 13 Cuts at: 894 968 1086 Size: 74 118 Fragments arranged by size: 244 200 195 118 115 74 63 46 18 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 458 543 723 785 1086 Size: 458 85 180 62 301 Fragments arranged by size: 458 301 180 85 62 BsrFI r'CCGG_y Cuts at: 0 461 1086 Size: 461 625 BssSI C'ACGA_G Cuts at: 0 874 1086 Size: 874 212 BstAPI GCAn_nnn'nTGC Cuts at: 0 735 777 927 1086 Size: 735 42 150 159 Fragments arranged by size: 735 159 150 42 BstXI CCAn_nnnn'nTGG Cuts at: 0 439 825 1086 Size: 439 386 261 Fragments arranged by size: 439 386 261 BtgI C'CryG_G Cuts at: 0 170 818 1086 Size: 170 648 268 Fragments arranged by size: 648 268 170 BtsI GCAGTG_nn' Cuts at: 0 326 1086 Size: 326 760 EaeI y'GGCC_r Cuts at: 0 161 350 389 1086 Size: 161 189 39 697 Fragments arranged by size: 697 189 161 39 EagI C'GGCC_G Cuts at: 0 350 1086 Size: 350 736 EarI CTCTTCn'nnn_ Cuts at: 0 72 176 283 640 1086 Size: 72 104 107 357 446 Fragments arranged by size: 446 357 107 104 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 679 961 1086 Size: 679 282 125 Fragments arranged by size: 679 282 125 EcoICRI GAG'CTC Cuts at: 0 966 1086 Size: 966 120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 53 182 200 400 490 979 1041 1042 Size: 53 129 18 200 90 489 62 1 Cuts at: 1042 1086 Size: 44 Fragments arranged by size: 489 200 129 90 62 53 44 18 1 EcoO109I rG'GnC_Cy Cuts at: 0 374 375 630 806 1086 Size: 374 1 255 176 280 Fragments arranged by size: 374 280 255 176 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 651 1086 Size: 651 435 HaeII r_GCGC'y Cuts at: 0 43 1086 Size: 43 1043 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 30 229 261 688 720 1086 Size: 30 199 32 427 32 366 Fragments arranged by size: 427 366 199 32 32 30 KpnI G_GTAC'C Cuts at: 0 468 1086 Size: 468 618 MscI TGG'CCA Cuts at: 0 163 1086 Size: 163 923 MslI CAynn'nnrTG Cuts at: 0 823 1086 Size: 823 263 MspA1I CmG'CkG Cuts at: 0 172 267 435 837 980 987 1066 1086 Size: 172 95 168 402 143 7 79 20 Fragments arranged by size: 402 172 168 143 95 79 20 7 NcoI C'CATG_G Cuts at: 0 818 1086 Size: 818 268 NspI r_CATG'y Cuts at: 0 447 1086 Size: 447 639 PasI CC'CwG_GG Cuts at: 0 57 1086 Size: 57 1029 PflMI CCAn_nnn'nTGG Cuts at: 0 837 1086 Size: 837 249 PfoI T'CCnGG_A Cuts at: 0 545 1086 Size: 545 541 PmlI CAC'GTG Cuts at: 0 521 882 1086 Size: 521 361 204 Fragments arranged by size: 521 361 204 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1009 1041 1086 Size: 1009 32 45 Fragments arranged by size: 1009 45 32 PpuMI rG'GwC_Cy Cuts at: 0 806 1086 Size: 806 280 PspOMI G'GGCC_C Cuts at: 0 374 1086 Size: 374 712 PstI C_TGCA'G Cuts at: 0 158 236 683 773 995 1086 Size: 158 78 447 90 222 91 Fragments arranged by size: 447 222 158 91 90 78 PvuII CAG'CTG Cuts at: 0 435 837 987 1086 Size: 435 402 150 99 Fragments arranged by size: 435 402 150 99 SacI G_AGCT'C Cuts at: 0 968 1086 Size: 968 118 SacII CC_GC'GG Cuts at: 0 173 1086 Size: 173 913 SanDI GG'GwC_CC Cuts at: 0 806 1086 Size: 806 280 SapI GCTCTTCn'nnn_ Cuts at: 0 72 176 283 1086 Size: 72 104 107 803 Fragments arranged by size: 803 107 104 72 SfcI C'TryA_G Cuts at: 0 154 232 679 769 991 1042 1086 Size: 154 78 447 90 222 51 44 Fragments arranged by size: 447 222 154 90 78 51 44 SmlI C'TyrA_G Cuts at: 0 15 745 1086 Size: 15 730 341 Fragments arranged by size: 730 341 15 SphI G_CATG'C Cuts at: 0 447 1086 Size: 447 639 StyI C'CwwG_G Cuts at: 0 144 818 1086 Size: 144 674 268 Fragments arranged by size: 674 268 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 556 1086 Size: 556 530 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 125 157 1086 Size: 125 32 929 Fragments arranged by size: 929 125 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 648 1086 Size: 648 438 Enzymes that do cut: AarI Acc65I AcuI AlfI AloI AlwNI ApaI AvaI BanI BanII BglI Bme1580I BmrI BpmI BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssSI BstAPI BstXI BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KpnI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SfcI SmlI SphI StyI TaqII TstI XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaWI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI