Restriction map for
pFN21AE0786
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BalI BamHI
BbsI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaBI BsaI
BsiWI BsoBI Bsp1407I BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI DraI DraIII Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluNI
MscI MseI MunI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 1121 TCCGGA T^CCGGA
AflIII 896 ACGCGT A^C[GA][CT]GT
AhdI 410 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1084 GTGCAC G^TGCAC
AlwI 207 GATCC GGATC(4/5)
AlwNI 436 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 695 AGCGCT AGC^GCT
ApaI 248 GGGCCC GGGCC^C
ApaLI 1084 GTGCAC G^TGCAC
AspEI 410 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1081 CACGTG CAC^GTG
BglI 124 GCCGGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 150 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 150 GCTGAGC GC^T[ACGT]AGC
BsaAI 1081 CACGTG [CT]AC^GT[GA]
BsaWI 1121 TCCGGA [AT]^CCGG[AT]
BseAI 1121 TCCGGA T^CCGGA
BsgI 784 GTGCAG GTGCAG(16/14)
BsmBI 327 CGTCTC CGTCTC(1/5)
BsmI 168 GAATGC GAATGC(1/-1)
BspDI 157 ATCGAT AT^CGAT
BspEI 1121 TCCGGA T^CCGGA
BspMI 1270 ACCTGC ACCTGC(4/8)
BsrBI 403 CCGCTC CCGCTC(-3/-3)
BstAPI 1190 GCACGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 31 CCAGCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 206 AGATCC [GA]^GATC[CT]
CelII 150 GCTGAGC GC^T[ACGT]AGC
ClaI 157 ATCGAT AT^CGAT
DrdI 480 GACAGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 410 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 946 GAAGAG CTCTTC(1/4)
Eco47III 695 AGCGCT AGC^GCT
EcoNI 942 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Ksp632I 946 GAAGAG CTCTTC(1/4)
MflI 206 AGATCC [GA]^GATC[CT]
MluI 896 ACGCGT A^CGCGT
MroI 1121 TCCGGA T^CCGGA
MslI 1201 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 744 GCCGGC GCC^GGC
NcoI 1200 CCATGG C^CATGG
NgoMIV 744 GCCGGC G^CCGGC
NotI 465 GCGGCCGC GC^GGCCGC
PleI 834 GACTC GAGTC(4/5)
PmaCI 1081 CACGTG CAC^GTG
PmlI 1081 CACGTG CAC^GTG
PpuMI 938 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 763 CTGCAG CTGCA^G
SexAI 878 ACCTGGT A^CC[AT]GGT
SfcI 763 CTGCAG C^T[GA][CT]AG
SfiI 123 GGCCGGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 4 GATTC G^A[AT]TC
XhoII 206 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 309 GTAC GT^AC
900 GTAC
BanII 248 GGGCCC G[GA]GC[CT]^C
622 GAGCCC
BbeI 360 GGCGCC GGCGC^C
973 GGCGCC
BbvCI 228 CCTCAGC CCTCAGC(-5/-2)
240 CCTCAGC
BcgI 693 CGAGCGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
716 CGAGCACAGTGC
BpmI 22 CTGGAG CTGGAG(16/14)
1240 CTGGAG
BsmAI 1169 GAGAC GTCTC(1/5)
328 GTCTC
BsrFI 255 GCCGGT [GA]^CCGG[CT]
744 GCCGGC
Cfr10I 255 GCCGGT [GA]^CCGG[CT]
744 GCCGGC
EagI 466 CGGCCG C^GGCCG
472 CGGCCG
EclXI 466 CGGCCG C^GGCCG
472 CGGCCG
Eco52I 466 CGGCCG C^GGCCG
472 CGGCCG
EcoT14I 37 CCTTGG C^C[AT][AT]GG
1200 CCATGG
HinfI 4 GATTC G^A[ACGT]TC
834 GACTC
HphI 139 GGTGA GGTGA(8/7)
846 TCACC
KasI 360 GGCGCC G^GCGCC
973 GGCGCC
MaeII 1001 ACGT A^CGT
1082 ACGT
NarI 360 GGCGCC GG^CGCC
973 GGCGCC
RsaI 309 GTAC GT^AC
900 GTAC
SfoI 360 GGCGCC GGC^GCC
973 GGCGCC
StuI 278 AGGCCT AGG^CCT
1336 AGGCCT
StyI 37 CCTTGG C^C[AT][AT]GG
1200 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaII BanI BbvI BcnI BmyI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI KspI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PvuII SacII Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I TspRI
TthHB8I