(Circular) (Six-base) MAPSORT of: pFN21AE0786.seq Check: 4504 from: 1 to: 6076 pFN21AE0786 6076 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3284 3284 Size: 6076 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3886 278 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3419 1937 Size: 1482 4594 Acc65I G'GTAC_C Cuts at: 3397 3397 Size: 6076 AclI AA'CG_TT Cuts at: 1563 4203 4576 1563 Size: 2640 373 3063 Fragments arranged by size: 3063 2640 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 3092 3244 4139 5523 5791 1632 Size: 953 507 152 895 1384 268 1917 Fragments arranged by size: 1917 1384 953 895 507 268 152 AfeI AGC'GCT Cuts at: 2702 2702 Size: 6076 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5879 AflIII A'CryG_T Cuts at: 1203 2901 4975 5952 1203 Size: 1698 2074 977 1327 Fragments arranged by size: 2074 1698 1327 977 AgeI A'CCGG_T Cuts at: 4907 4907 Size: 6076 AhdI GACnn_n'nnGTC Cuts at: 2420 4805 2420 Size: 2385 3691 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6076 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2814 1184 Size: 1630 4446 AlwNI CAG_nnn'CTG Cuts at: 2446 3819 5391 2446 Size: 1373 1572 3131 Fragments arranged by size: 3131 1572 1373 ApaI G_GGCC'C Cuts at: 2257 2257 Size: 6076 ApaLI G'TGCA_C Cuts at: 3089 4133 5289 3089 Size: 1044 1156 3876 Fragments arranged by size: 3876 1156 1044 ApoI r'AATT_y Cuts at: 1493 3385 3611 3763 5872 1493 Size: 1892 226 152 2109 1697 Fragments arranged by size: 2109 1892 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4630 160 Size: 872 3598 1606 Fragments arranged by size: 3598 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 AvaI C'yCGr_G Cuts at: 1870 1943 3401 1870 Size: 73 1458 4545 Fragments arranged by size: 4545 1458 73 BamHI G'GATC_C Cuts at: 3406 3773 3406 Size: 367 5709 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2365 2528 2581 2809 2978 Size: 325 217 627 578 163 53 228 169 Cuts at: 2978 3397 4852 618 Size: 419 1455 1842 Fragments arranged by size: 1842 1455 627 578 419 325 228 217 169 163 53 BanII G_rGCy'C Cuts at: 1391 2257 2631 3395 1391 Size: 866 374 764 4072 Fragments arranged by size: 4072 866 764 374 BbeI G_GCGC'C Cuts at: 2369 2982 2369 Size: 613 5463 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5105 BbvCI CC'TCA_GC Cuts at: 2234 2246 2234 Size: 12 6064 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2687 2710 2721 2744 4267 4301 1903 Size: 34 750 23 11 23 1523 34 3678 Fragments arranged by size: 3678 1523 750 34 34 23 23 11 BciVI GTATCCnnnnn_n' Cuts at: 3968 5178 3968 Size: 1210 4866 BclI T'GATC_A Cuts at: 1548 1548 Size: 6076 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2135 4687 136 Size: 107 122 71 631 605 463 2552 1525 Fragments arranged by size: 2552 1525 631 605 463 122 107 71 BglII A'GATC_T Cuts at: 6071 6071 Size: 6076 BlpI GC'TnA_GC Cuts at: 2156 3493 2156 Size: 1337 4739 Bme1580I G_kGCm'C Cuts at: 1790 2085 2257 2586 3093 3162 4137 5293 1790 Size: 295 172 329 507 69 975 1156 2573 Fragments arranged by size: 2573 1156 975 507 329 295 172 69 BmgBI CAC'GTC Cuts at: 1318 3007 1318 Size: 1689 4387 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2158 3807 4765 Size: 416 53 83 399 24 741 1649 958 Cuts at: 4765 442 Size: 1753 Fragments arranged by size: 1753 1649 958 741 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6076 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 3266 4736 1239 Size: 809 1218 1470 2579 Fragments arranged by size: 2579 1470 1218 809 Bpu10I CC'TnA_GC Cuts at: 2234 2246 2234 Size: 12 6064 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4201 5066 5364 4201 Size: 865 298 4913 Fragments arranged by size: 4913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4739 882 Size: 617 3240 2219 Fragments arranged by size: 3240 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3088 3867 493 Size: 711 1884 779 2702 Fragments arranged by size: 2702 1884 779 711 BsaBI GATnn'nnATC Cuts at: 6070 6070 Size: 6076 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2366 2979 3119 3883 Size: 53 83 186 936 833 613 140 764 Cuts at: 3883 4265 4900 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 833 764 635 613 382 186 140 83 53 BsaWI w'CCGG_w Cuts at: 3126 4509 4907 5181 5328 5739 3126 Size: 1383 398 274 147 411 3463 Fragments arranged by size: 3463 1383 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2124 2154 2394 2424 2909 Size: 30 165 30 721 30 240 30 485 Cuts at: 2909 2939 1178 Size: 30 4315 Fragments arranged by size: 4315 721 485 240 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2570 2930 2984 3164 1186 Size: 1384 360 54 180 4098 Fragments arranged by size: 4098 1384 360 180 54 BseYI C'CCAG_C Cuts at: 2256 2327 2599 2605 2651 2893 3020 3230 5279 Size: 71 272 6 46 242 127 210 2049 Cuts at: 5279 5775 2256 Size: 496 2557 Fragments arranged by size: 2557 2049 496 272 242 210 127 71 46 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2810 1163 Size: 573 1074 4429 Fragments arranged by size: 4429 1074 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2417 2474 2480 3526 4287 5315 Size: 275 186 416 57 6 1046 761 1028 Cuts at: 5315 1540 Size: 2301 Fragments arranged by size: 2301 1046 1028 761 416 275 186 57 6 BsiHKAI G_wGCw'C Cuts at: 2562 2726 3093 4137 4222 5293 2562 Size: 164 367 1044 85 1071 3345 Fragments arranged by size: 3345 1071 1044 367 164 85 BsmI GAATG_Cn' Cuts at: 2179 3587 3680 2179 Size: 1408 93 4575 Fragments arranged by size: 4575 1408 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2338 2338 Size: 6076 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2257 2562 2586 2631 2726 3093 Size: 399 295 172 305 24 45 95 367 Cuts at: 3093 3162 3395 4137 4222 5293 1391 Size: 69 233 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 367 305 295 233 172 95 85 69 45 24 BspEI T'CCGG_A Cuts at: 3126 5739 3126 Size: 2613 3463 BspHI T'CATG_A Cuts at: 3965 5695 3965 Size: 1730 4346 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3284 3431 844 Size: 2440 147 3489 Fragments arranged by size: 3489 2440 147 BsrBI CCG'CTC Cuts at: 1394 1823 2410 3963 1394 Size: 429 587 1553 3507 Fragments arranged by size: 3507 1553 587 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4571 4745 65 Size: 1183 3323 174 1396 Fragments arranged by size: 3323 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2260 2749 4720 4907 1700 Size: 110 450 489 1971 187 2869 Fragments arranged by size: 2869 1971 489 450 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6076 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6076 BssSI C'ACGA_G Cuts at: 4136 5148 4136 Size: 1012 5064 BstAPI GCAn_nnn'nTGC Cuts at: 794 3201 794 Size: 2407 3669 BstBI TT'CG_AA Cuts at: 4880 4880 Size: 6076 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6076 BstXI CCAn_nnnn'nTGG Cuts at: 2043 2043 Size: 6076 BstYI r'GATC_y Cuts at: 1972 2211 3406 3773 4158 4175 5627 5713 5725 Size: 239 1195 367 385 17 1452 86 12 Cuts at: 5725 6071 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1195 385 367 346 239 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2139 2298 2309 2474 3205 513 Size: 552 939 135 159 11 165 731 3384 Fragments arranged by size: 3384 939 731 552 165 159 135 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5337 Fragments arranged by size: 5337 683 56 BtsI GCAGTG_nn' Cuts at: 3091 3592 4386 4406 3091 Size: 501 794 20 4761 Fragments arranged by size: 4761 794 501 20 ClaI AT'CG_AT Cuts at: 1554 2163 1554 Size: 609 5467 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6041 DraI TTT'AAA Cuts at: 3380 3380 Size: 6076 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2491 5083 809 Size: 507 1175 2592 1802 Fragments arranged by size: 2592 1802 1175 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2471 2477 Size: 54 1425 50 275 18 306 335 6 Cuts at: 2477 2751 3523 4412 6041 8 Size: 274 772 889 1629 43 Fragments arranged by size: 1629 1425 889 772 335 306 275 274 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2471 2477 3523 1537 Size: 275 659 6 1046 4090 Fragments arranged by size: 4090 1046 659 275 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2946 4006 1353 Size: 1593 1060 3423 Fragments arranged by size: 3423 1593 1060 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2278 4663 5037 5183 387 Size: 1064 138 689 2385 374 146 1280 Fragments arranged by size: 2385 1280 1064 689 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2585 3092 3244 3266 4139 4736 Size: 393 416 537 507 152 22 873 597 Cuts at: 4736 5523 5791 1239 Size: 787 268 1524 Fragments arranged by size: 1524 873 787 597 537 507 416 393 268 152 22 EcoNI CCTnn'n_nnAGG Cuts at: 2951 2951 Size: 6076 EcoO109I rG'GnC_Cy Cuts at: 2253 2944 3135 3250 2253 Size: 691 191 115 5079 Fragments arranged by size: 5079 691 191 115 EcoRI G'AATT_C Cuts at: 3385 3763 3385 Size: 378 5698 FspI TGC'GCA Cuts at: 4582 4582 Size: 6076 HaeII r_GCGC'y Cuts at: 1678 2233 2369 2388 2704 2894 2982 5223 1678 Size: 555 136 19 316 190 88 2241 2531 Fragments arranged by size: 2531 2241 555 316 190 136 88 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2394 2406 2426 2438 4717 Size: 32 135 32 822 12 20 12 2279 Cuts at: 4717 4749 4791 4823 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2279 822 135 42 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3420 3666 669 Size: 988 281 1482 246 3079 Fragments arranged by size: 3079 1482 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6076 HpaI GTT'AAC Cuts at: 3666 3666 Size: 6076 KasI G'GCGC_C Cuts at: 2365 2978 2365 Size: 613 5463 KpnI G_GTAC'C Cuts at: 3401 3401 Size: 6076 MfeI C'AATT_G Cuts at: 3675 3675 Size: 6076 MluI A'CGCG_T Cuts at: 2901 5952 2901 Size: 3051 3025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2437 5190 5374 1305 Size: 1132 2753 184 2007 Fragments arranged by size: 2753 2007 1132 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4254 Fragments arranged by size: 4254 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3210 4034 4393 4552 518 Size: 648 2044 824 359 159 2042 Fragments arranged by size: 2044 2042 824 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2300 2311 2378 2419 2443 2476 2767 2798 Size: 694 11 67 41 24 33 291 31 Cuts at: 2798 3024 3264 3492 4169 5317 5562 1606 Size: 226 240 228 677 1148 245 2120 Fragments arranged by size: 2120 1148 694 677 291 245 240 228 226 67 41 33 31 24 11 NaeI GCC'GGC Cuts at: 2751 2751 Size: 6076 NarI GG'CG_CC Cuts at: 2366 2979 2366 Size: 613 5463 NcoI C'CATG_G Cuts at: 513 1065 2004 3205 513 Size: 552 939 1201 3384 Fragments arranged by size: 3384 1201 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6076 NgoMIV G'CCGG_C Cuts at: 2749 2749 Size: 6076 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6076 NotI GC'GGCC_GC Cuts at: 2471 3523 2471 Size: 1052 5024 NspI r_CATG'y Cuts at: 1132 1229 3434 4979 1132 Size: 97 2205 1545 2229 Fragments arranged by size: 2229 2205 1545 97 PasI CC'CwG_GG Cuts at: 1348 2673 3253 1348 Size: 1325 580 4171 Fragments arranged by size: 4171 1325 580 PciI A'CATG_T Cuts at: 4975 4975 Size: 6076 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6076 PfoI T'CCnGG_A Cuts at: 2946 3243 2946 Size: 297 5779 PmeI GTTT'AAAC Cuts at: 3380 3380 Size: 6076 PmlI CAC'GTG Cuts at: 3088 3088 Size: 6076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4145 4177 5658 5690 4145 Size: 32 1481 32 4531 Fragments arranged by size: 4531 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2944 2944 Size: 6076 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6076 PsiI TTA'TAA Cuts at: 3646 3646 Size: 6076 PspOMI G'GGCC_C Cuts at: 2253 2253 Size: 6076 PstI C_TGCA'G Cuts at: 830 2772 3428 830 Size: 1942 656 3478 Fragments arranged by size: 3478 1942 656 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6076 PvuII CAG'CTG Cuts at: 2798 3024 3264 2798 Size: 226 240 5610 Fragments arranged by size: 5610 240 226 SacII CC_GC'GG Cuts at: 2301 2312 2477 2301 Size: 11 165 5900 Fragments arranged by size: 5900 165 11 SalI G'TCGA_C Cuts at: 1936 3418 1936 Size: 1482 4594 SbfI CC_TGCA'GG Cuts at: 3428 3428 Size: 6076 ScaI AGT'ACT Cuts at: 4324 6036 4324 Size: 1712 4364 SexAI A'CCwGG_T Cuts at: 2883 2883 Size: 6076 SfcI C'TryA_G Cuts at: 826 1045 2768 3424 4559 5240 5431 826 Size: 219 1723 656 1135 681 191 1471 Fragments arranged by size: 1723 1471 1135 681 656 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2135 2135 Size: 6076 SfoI GGC'GCC Cuts at: 2367 2980 2367 Size: 613 5463 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6076 SmaI CCC'GGG Cuts at: 1872 3403 1872 Size: 1531 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4180 5081 5343 820 Size: 197 926 2237 901 262 1553 Fragments arranged by size: 2237 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3867 493 Size: 3374 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6076 SphI G_CATG'C Cuts at: 1132 3434 1132 Size: 2302 3774 SspI AAT'ATT Cuts at: 5 52 4000 5 Size: 47 3948 2081 Fragments arranged by size: 3948 2081 47 StuI AGG'CCT Cuts at: 1517 2285 3343 1517 Size: 768 1058 4250 Fragments arranged by size: 4250 1058 768 StyI C'CwwG_G Cuts at: 513 1065 2004 2042 3205 3515 513 Size: 552 939 38 1163 310 3074 Fragments arranged by size: 3074 1163 939 552 310 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2132 2140 2341 3034 4103 4120 4273 Size: 1255 552 8 201 693 1069 17 153 Cuts at: 4273 4458 4903 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1069 693 552 445 201 185 153 17 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4322 6034 96 Size: 274 80 33 51 1443 2345 1712 138 Fragments arranged by size: 2345 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2124 2156 2909 2941 6036 6068 2124 Size: 32 753 32 3095 32 2132 Fragments arranged by size: 3095 2132 753 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6076 XbaI T'CTAG_A Cuts at: 3412 3412 Size: 6076 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6076 XmaI C'CCGG_G Cuts at: 1870 3401 1870 Size: 1531 4545 XmnI GAAnn'nnTTC Cuts at: 4205 4205 Size: 6076 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3884 276 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SanDI SapI SrfI SwaI XcmI