Restriction map for
pFN21AE0786
(Linear) (Six-base) MAPSORT of: KIEE0786.seq Check: 7047 from: 1 to: 1371
KIEE0786.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1279 1371
Size: 1279 92
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1087 1239 1371
Size: 580 507 152 132
Fragments arranged by size:
580 507 152 132
AfeI AGC'GCT
Cuts at: 0 697 1371
Size: 697 674
AflIII A'CryG_T
Cuts at: 0 896 1371
Size: 896 475
AhdI GACnn_n'nnGTC
Cuts at: 0 415 1371
Size: 415 956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 809 1371
Size: 809 562
AlwNI CAG_nnn'CTG
Cuts at: 0 441 1371
Size: 441 930
ApaI G_GGCC'C
Cuts at: 0 252 1371
Size: 252 1119
ApaLI G'TGCA_C
Cuts at: 0 1084 1371
Size: 1084 287
BanI G'GyrC_C
Cuts at: 0 360 523 576 804 973 1371
Size: 360 163 53 228 169 398
Fragments arranged by size:
398 360 228 169 163 53
BanII G_rGCy'C
Cuts at: 0 252 626 1371
Size: 252 374 745
Fragments arranged by size:
745 374 252
BbeI G_GCGC'C
Cuts at: 0 364 977 1371
Size: 364 613 394
Fragments arranged by size:
613 394 364
BbvCI CC'TCA_GC
Cuts at: 0 229 241 1371
Size: 229 12 1130
Fragments arranged by size:
1130 229 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 682 705 716 739 1371
Size: 682 23 11 23 632
Fragments arranged by size:
682 632 23 23 11
BglI GCCn_nnn'nGGC
Cuts at: 0 130 1371
Size: 130 1241
BlpI GC'TnA_GC
Cuts at: 0 151 1371
Size: 151 1220
Bme1580I G_kGCm'C
Cuts at: 0 80 252 581 1088 1157 1371
Size: 80 172 329 507 69 214
Fragments arranged by size:
507 329 214 172 80 69
BmgBI CAC'GTC
Cuts at: 0 1002 1371
Size: 1002 369
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 1371
Size: 153 1218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1261 1371
Size: 43 1218 110
Fragments arranged by size:
1218 110 43
Bpu10I CC'TnA_GC
Cuts at: 0 229 241 1371
Size: 229 12 1130
Fragments arranged by size:
1130 229 12
BsaAI yAC'GTr
Cuts at: 0 1083 1371
Size: 1083 288
BsaHI Gr'CG_yC
Cuts at: 0 361 974 1114 1371
Size: 361 613 140 257
Fragments arranged by size:
613 361 257 140
BsaWI w'CCGG_w
Cuts at: 0 1121 1371
Size: 1121 250
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 119 149 389 419 904 934 1371
Size: 119 30 240 30 485 30 437
Fragments arranged by size:
485 437 240 119 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 565 925 979 1159 1371
Size: 565 360 54 180 212
Fragments arranged by size:
565 360 212 180 54
BseYI C'CCAG_C
Cuts at: 0 251 322 594 600 646 888 1015 1225
Size: 251 71 272 6 46 242 127 210
Cuts at: 1225 1371
Size: 146
Fragments arranged by size:
272 251 242 210 146 127 71 46
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 1371
Size: 805 566
BsiEI CG_ry'CG
Cuts at: 0 412 469 475 1371
Size: 412 57 6 896
Fragments arranged by size:
896 412 57 6
BsiHKAI G_wGCw'C
Cuts at: 0 557 721 1088 1371
Size: 557 164 367 283
Fragments arranged by size:
557 367 283 164
BsmI GAATG_Cn'
Cuts at: 0 174 1371
Size: 174 1197
BsmBI CGTCTCn'nnnn_
Cuts at: 0 333 1371
Size: 333 1038
Bsp1286I G_dGCh'C
Cuts at: 0 80 252 557 581 626 721 1088 1157
Size: 80 172 305 24 45 95 367 69
Cuts at: 1157 1371
Size: 214
Fragments arranged by size:
367 305 214 172 95 80 69 45
24
BspEI T'CCGG_A
Cuts at: 0 1121 1371
Size: 1121 250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1279 1371
Size: 1279 92
BsrBI CCG'CTC
Cuts at: 0 405 1371
Size: 405 966
BsrFI r'CCGG_y
Cuts at: 0 255 744 1371
Size: 255 489 627
Fragments arranged by size:
627 489 255
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1196 1371
Size: 1196 175
BstXI CCAn_nnnn'nTGG
Cuts at: 0 38 1371
Size: 38 1333
BstYI r'GATC_y
Cuts at: 0 206 1371
Size: 206 1165
BtgI C'CryG_G
Cuts at: 0 134 293 304 469 1200 1371
Size: 134 159 11 165 731 171
Fragments arranged by size:
731 171 165 159 134 11
BtsI GCAGTG_nn'
Cuts at: 0 1086 1371
Size: 1086 285
ClaI AT'CG_AT
Cuts at: 0 158 1371
Size: 158 1213
DrdI GACnn_nn'nnGTC
Cuts at: 0 486 1371
Size: 486 885
EaeI y'GGCC_r
Cuts at: 0 131 466 472 746 1371
Size: 131 335 6 274 625
Fragments arranged by size:
625 335 274 131 6
EagI C'GGCC_G
Cuts at: 0 466 472 1371
Size: 466 6 899
Fragments arranged by size:
899 466 6
EarI CTCTTCn'nnn_
Cuts at: 0 941 1371
Size: 941 430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 273 1371
Size: 273 1098
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 580 1087 1239 1261 1371
Size: 43 537 507 152 22 110
Fragments arranged by size:
537 507 152 110 43 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 946 1371
Size: 946 425
EcoO109I rG'GnC_Cy
Cuts at: 0 248 939 1130 1245 1371
Size: 248 691 191 115 126
Fragments arranged by size:
691 248 191 126 115
HaeII r_GCGC'y
Cuts at: 0 228 364 383 699 889 977 1371
Size: 228 136 19 316 190 88 394
Fragments arranged by size:
394 316 228 190 136 88 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 389 401 421 433 1371
Size: 389 12 20 12 938
Fragments arranged by size:
938 389 20 12 12
KasI G'GCGC_C
Cuts at: 0 360 973 1371
Size: 360 613 398
Fragments arranged by size:
613 398 360
MluI A'CGCG_T
Cuts at: 0 896 1371
Size: 896 475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1371
Size: 432 939
MslI CAynn'nnrTG
Cuts at: 0 1205 1371
Size: 1205 166
MspA1I CmG'CkG
Cuts at: 0 295 306 373 414 438 471 762 793
Size: 295 11 67 41 24 33 291 31
Cuts at: 793 1019 1259 1371
Size: 226 240 112
Fragments arranged by size:
295 291 240 226 112 67 41 33
31 24 11
NaeI GCC'GGC
Cuts at: 0 746 1371
Size: 746 625
NarI GG'CG_CC
Cuts at: 0 361 974 1371
Size: 361 613 397
Fragments arranged by size:
613 397 361
NcoI C'CATG_G
Cuts at: 0 1200 1371
Size: 1200 171
NgoMIV G'CCGG_C
Cuts at: 0 744 1371
Size: 744 627
NotI GC'GGCC_GC
Cuts at: 0 466 1371
Size: 466 905
PasI CC'CwG_GG
Cuts at: 0 668 1248 1371
Size: 668 580 123
Fragments arranged by size:
668 580 123
PfoI T'CCnGG_A
Cuts at: 0 941 1238 1371
Size: 941 297 133
Fragments arranged by size:
941 297 133
PmlI CAC'GTG
Cuts at: 0 1083 1371
Size: 1083 288
PpuMI rG'GwC_Cy
Cuts at: 0 939 1371
Size: 939 432
PspOMI G'GGCC_C
Cuts at: 0 248 1371
Size: 248 1123
PstI C_TGCA'G
Cuts at: 0 767 1371
Size: 767 604
PvuII CAG'CTG
Cuts at: 0 793 1019 1259 1371
Size: 793 226 240 112
Fragments arranged by size:
793 240 226 112
SacII CC_GC'GG
Cuts at: 0 296 307 472 1371
Size: 296 11 165 899
Fragments arranged by size:
899 296 165 11
SexAI A'CCwGG_T
Cuts at: 0 878 1371
Size: 878 493
SfcI C'TryA_G
Cuts at: 0 763 1371
Size: 763 608
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 130 1371
Size: 130 1241
SfoI GGC'GCC
Cuts at: 0 362 975 1371
Size: 362 613 396
Fragments arranged by size:
613 396 362
StuI AGG'CCT
Cuts at: 0 280 1338 1371
Size: 280 1058 33
Fragments arranged by size:
1058 280 33
StyI C'CwwG_G
Cuts at: 0 37 1200 1371
Size: 37 1163 171
Fragments arranged by size:
1163 171 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 127 135 336 1029 1371
Size: 127 8 201 693 342
Fragments arranged by size:
693 342 201 127 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 119 151 904 936 1371
Size: 119 32 753 32 435
Fragments arranged by size:
753 435 119 32 32
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AlfI AlwNI ApaI
ApaLI BanI BanII BbeI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI BstXI BstYI
BtgI BtsI ClaI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MluI MmeI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI PasI
PfoI PmlI PpuMI PspOMI PstI PvuII SacII SexAI
SfcI SfiI SfoI StuI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AleI AloI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbsI BciVI BclI BfrBI BglII BmtI BplI BpuEI
BsaI BsaBI BsiWI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MscI NdeI NheI NruI
NsiI NspI PacI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI