(Circular) (Six-base) MAPSORT of: pFN21AE4440.seq Check: 8433 from: 1 to: 6076 pFN21AE4440 6076 bp With 182 enzymes: SgfI * March 28, 2012 14:01 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3886 278 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3419 1937 Size: 1482 4594 Acc65I G'GTAC_C Cuts at: 3397 3397 Size: 6076 AclI AA'CG_TT Cuts at: 1563 4203 4576 1563 Size: 2640 373 3063 Fragments arranged by size: 3063 2640 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 3136 4139 5523 5791 1632 Size: 620 884 1003 1384 268 1917 Fragments arranged by size: 1917 1384 1003 884 620 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5879 AflIII A'CryG_T Cuts at: 1203 4975 5952 1203 Size: 3772 977 1327 Fragments arranged by size: 3772 1327 977 AgeI A'CCGG_T Cuts at: 4907 4907 Size: 6076 AhdI GACnn_n'nnGTC Cuts at: 4805 4805 Size: 6076 AleI CACnn'nnGTG Cuts at: 1166 2759 1166 Size: 1593 4483 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2070 1184 Size: 886 5190 AlwNI CAG_nnn'CTG Cuts at: 2632 3304 3819 5391 2632 Size: 672 515 1572 3317 Fragments arranged by size: 3317 1572 672 515 ApaI G_GGCC'C Cuts at: 3139 3161 3139 Size: 22 6054 ApaLI G'TGCA_C Cuts at: 4133 5289 4133 Size: 1156 4920 ApoI r'AATT_y Cuts at: 1493 2224 3385 3611 3763 5872 1493 Size: 731 1161 226 152 2109 1697 Fragments arranged by size: 2109 1697 1161 731 226 152 AseI AT'TA_AT Cuts at: 160 1032 4630 160 Size: 872 3598 1606 Fragments arranged by size: 3598 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 AvaI C'yCGr_G Cuts at: 1870 1943 2738 2776 3138 3198 3248 3401 1870 Size: 73 795 38 362 60 50 153 4545 Fragments arranged by size: 4545 795 362 153 73 60 50 38 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2082 2115 2082 Size: 33 6043 BamHI G'GATC_C Cuts at: 2199 3406 3773 2199 Size: 1207 367 4502 Fragments arranged by size: 4502 1207 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2261 3397 4852 618 Size: 325 217 627 474 1136 1455 1842 Fragments arranged by size: 1842 1455 1136 627 474 325 217 BanII G_rGCy'C Cuts at: 1391 2241 2475 3139 3161 3395 1391 Size: 850 234 664 22 234 4072 Fragments arranged by size: 4072 850 664 234 234 22 BbeI G_GCGC'C Cuts at: 2265 2265 Size: 6076 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5105 BbvCI CC'TCA_GC Cuts at: 2493 2715 2859 2493 Size: 222 144 5710 Fragments arranged by size: 5710 222 144 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4267 4301 1903 Size: 34 50 34 2246 34 3678 Fragments arranged by size: 3678 2246 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2083 3968 5178 2083 Size: 1885 1210 2981 Fragments arranged by size: 2981 1885 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6076 BfrBI ATG'CAT Cuts at: 2109 2109 Size: 6076 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2671 3326 4687 Size: 107 122 71 631 605 999 655 1361 Cuts at: 4687 136 Size: 1525 Fragments arranged by size: 1525 1361 999 655 631 605 122 107 71 BglII A'GATC_T Cuts at: 6071 6071 Size: 6076 BlpI GC'TnA_GC Cuts at: 3493 3493 Size: 6076 Bme1580I G_kGCm'C Cuts at: 1790 2278 3139 3161 4137 5293 1790 Size: 488 861 22 976 1156 2573 Fragments arranged by size: 2573 1156 976 861 488 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6076 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3807 4765 442 Size: 416 53 83 399 24 2390 958 1753 Fragments arranged by size: 2390 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6076 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2140 2638 3016 2140 Size: 498 378 5200 Fragments arranged by size: 5200 498 378 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2544 2742 2999 3049 3098 3205 3215 3326 Size: 1305 198 257 50 49 107 10 111 Cuts at: 3326 3356 4736 1239 Size: 30 1380 2579 Fragments arranged by size: 2579 1380 1305 257 198 111 107 50 49 30 10 Bpu10I CC'TnA_GC Cuts at: 2493 2715 2859 2868 2493 Size: 222 144 9 5701 Fragments arranged by size: 5701 222 144 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2348 3233 4201 5066 5364 2348 Size: 885 968 865 298 3060 Fragments arranged by size: 3060 968 885 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4739 882 Size: 617 3240 2219 Fragments arranged by size: 3240 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3867 493 Size: 711 2663 2702 Fragments arranged by size: 2702 2663 711 BsaBI GATnn'nnATC Cuts at: 6070 6070 Size: 6076 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 3883 4265 4900 Size: 53 83 186 936 729 1621 382 635 Cuts at: 4900 275 Size: 1451 Fragments arranged by size: 1621 1451 936 729 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2799 4509 4907 5181 5328 5739 2799 Size: 1710 398 274 147 411 3136 Fragments arranged by size: 3136 1710 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2542 2572 3299 3329 1178 Size: 30 165 30 1139 30 727 30 3925 Fragments arranged by size: 3925 1139 727 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2202 2540 2543 2546 2549 2552 2705 2708 Size: 1016 338 3 3 3 3 153 3 Cuts at: 2708 2711 2714 2717 2720 2723 2741 2876 2930 Size: 3 3 3 3 3 18 135 54 Cuts at: 2930 2933 2936 2939 3002 3005 3104 3107 3110 Size: 3 3 3 63 3 99 3 3 Cuts at: 3110 3266 3278 3281 1186 Size: 156 12 3 3981 Fragments arranged by size: 3981 1016 338 156 153 135 99 63 54 18 12 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2144 2573 2843 2904 5279 5775 2144 Size: 429 270 61 2375 496 2445 Fragments arranged by size: 2445 2375 496 429 270 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2679 1163 Size: 573 943 4560 Fragments arranged by size: 4560 943 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2819 3526 4287 5315 1540 Size: 275 186 818 707 761 1028 2301 Fragments arranged by size: 2301 1028 818 761 707 275 186 BsiHKAI G_wGCw'C Cuts at: 2241 2901 4137 4222 5293 2241 Size: 660 1236 85 1071 3024 Fragments arranged by size: 3024 1236 1071 660 85 BsmI GAATG_Cn' Cuts at: 3587 3680 3587 Size: 93 5983 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 2278 2475 2901 3139 3161 3395 Size: 399 451 37 197 426 238 22 234 Cuts at: 3395 4137 4222 5293 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 451 426 399 238 234 197 85 37 22 BspEI T'CCGG_A Cuts at: 2799 5739 2799 Size: 2940 3136 BspHI T'CATG_A Cuts at: 3965 5695 3965 Size: 1730 4346 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3431 844 Size: 2587 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2970 2995 3963 1394 Size: 429 1147 25 968 3507 Fragments arranged by size: 3507 1147 968 429 25 BsrDI GCAATG_nn' Cuts at: 65 1248 4571 4745 65 Size: 1183 3323 174 1396 Fragments arranged by size: 3323 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4720 4907 1700 Size: 110 2910 187 2869 Fragments arranged by size: 2910 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6076 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6076 BssSI C'ACGA_G Cuts at: 4136 5148 4136 Size: 1012 5064 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6076 BstBI TT'CG_AA Cuts at: 4880 4880 Size: 6076 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6076 BstYI r'GATC_y Cuts at: 1972 2199 2796 3406 3773 4158 4175 5627 5713 Size: 227 597 610 367 385 17 1452 86 Cuts at: 5713 5725 6071 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 610 597 385 367 346 227 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5524 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5337 Fragments arranged by size: 5337 683 56 BtsI GCAGTG_nn' Cuts at: 2052 2940 3592 4386 4406 2052 Size: 888 652 794 20 3722 Fragments arranged by size: 3722 888 794 652 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6076 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6041 DraI TTT'AAA Cuts at: 3380 3380 Size: 6076 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5083 809 Size: 507 3767 1802 Fragments arranged by size: 3767 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3048 3318 3523 Size: 54 1425 50 275 18 1218 270 205 Cuts at: 3523 4412 6041 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1218 889 275 270 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3523 1537 Size: 275 1711 4090 Fragments arranged by size: 4090 1711 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2034 2364 2517 2682 2957 2960 4006 1353 Size: 681 330 153 165 275 3 1046 3423 Fragments arranged by size: 3423 1046 681 330 275 165 153 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2002 4663 5037 5183 387 Size: 1064 138 413 2661 374 146 1280 Fragments arranged by size: 2661 1280 1064 413 374 146 138 EcoICRI GAG'CTC Cuts at: 2239 2239 Size: 6076 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2544 2742 2999 3049 3098 3136 Size: 393 620 292 198 257 50 49 38 Cuts at: 3136 3205 3215 3326 3356 4139 4736 5523 5791 Size: 69 10 111 30 783 597 787 268 Cuts at: 5791 1239 Size: 1524 Fragments arranged by size: 1524 787 783 620 597 393 292 268 257 198 111 69 50 49 38 30 10 EcoNI CCTnn'n_nnAGG Cuts at: 2624 2624 Size: 6076 EcoO109I rG'GnC_Cy Cuts at: 2454 2461 2509 2617 2650 2750 2872 3157 3158 Size: 7 48 108 33 100 122 285 1 Cuts at: 3158 2454 Size: 5372 Fragments arranged by size: 5372 285 122 108 100 48 33 7 1 EcoRI G'AATT_C Cuts at: 3385 3763 3385 Size: 378 5698 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3145 3145 Size: 6076 FspI TGC'GCA Cuts at: 4582 4582 Size: 6076 HaeII r_GCGC'y Cuts at: 1678 2265 5223 1678 Size: 587 2958 2531 Fragments arranged by size: 2958 2531 587 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2263 2295 2300 2332 2363 Size: 32 135 32 691 32 5 32 31 Cuts at: 2363 2395 2801 2833 3299 3331 4717 4749 4791 Size: 32 406 32 466 32 1386 32 42 Cuts at: 4791 4823 1373 Size: 32 2626 Fragments arranged by size: 2626 1386 691 466 406 135 42 32 32 32 32 32 32 32 32 32 31 5 HincII GTy'rAC Cuts at: 669 1657 1938 3420 3666 669 Size: 988 281 1482 246 3079 Fragments arranged by size: 3079 1482 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6076 HpaI GTT'AAC Cuts at: 3666 3666 Size: 6076 KasI G'GCGC_C Cuts at: 2261 2261 Size: 6076 KpnI G_GTAC'C Cuts at: 3401 3401 Size: 6076 MfeI C'AATT_G Cuts at: 3675 3675 Size: 6076 MluI A'CGCG_T Cuts at: 5952 5952 Size: 6076 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2151 2799 5190 5374 1305 Size: 846 648 2391 184 2007 Fragments arranged by size: 2391 2007 846 648 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3050 10 Size: 54 1425 343 1218 3036 Fragments arranged by size: 3036 1425 1218 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2759 4034 4393 4552 518 Size: 648 1593 1275 359 159 2042 Fragments arranged by size: 2042 1593 1275 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2298 3046 3172 3355 3492 4169 5317 Size: 412 280 748 126 183 137 677 1148 Cuts at: 5317 5562 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 748 677 412 280 245 183 137 126 NarI GG'CG_CC Cuts at: 2262 2262 Size: 6076 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5524 NdeI CA'TA_TG Cuts at: 387 387 Size: 6076 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6076 NotI GC'GGCC_GC Cuts at: 3523 3523 Size: 6076 NsiI A_TGCA'T Cuts at: 2111 2111 Size: 6076 NspI r_CATG'y Cuts at: 1132 1229 3434 4979 1132 Size: 97 2205 1545 2229 Fragments arranged by size: 2229 2205 1545 97 PasI CC'CwG_GG Cuts at: 1348 2513 3161 1348 Size: 1165 648 4263 Fragments arranged by size: 4263 1165 648 PciI A'CATG_T Cuts at: 4975 4975 Size: 6076 PflMI CCAn_nnn'nTGG Cuts at: 1108 3009 3027 3304 1108 Size: 1901 18 277 3880 Fragments arranged by size: 3880 1901 277 18 PfoI T'CCnGG_A Cuts at: 2466 2466 Size: 6076 PmeI GTTT'AAAC Cuts at: 3380 3380 Size: 6076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4145 4177 5658 5690 4145 Size: 32 1481 32 4531 Fragments arranged by size: 4531 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2872 2872 Size: 6076 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6076 PsiI TTA'TAA Cuts at: 3646 3646 Size: 6076 PspOMI G'GGCC_C Cuts at: 3135 3157 3135 Size: 22 6054 PstI C_TGCA'G Cuts at: 830 3360 3428 830 Size: 2530 68 3478 Fragments arranged by size: 3478 2530 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6076 PvuII CAG'CTG Cuts at: 3046 3172 3355 3046 Size: 126 183 5767 Fragments arranged by size: 5767 183 126 SacI G_AGCT'C Cuts at: 2241 2241 Size: 6076 SalI G'TCGA_C Cuts at: 1936 3418 1936 Size: 1482 4594 SapI GCTCTTCn'nnn_ Cuts at: 2034 2364 2960 2034 Size: 330 596 5150 Fragments arranged by size: 5150 596 330 SbfI CC_TGCA'GG Cuts at: 3428 3428 Size: 6076 ScaI AGT'ACT Cuts at: 4324 6036 4324 Size: 1712 4364 SfcI C'TryA_G Cuts at: 826 1045 2757 3356 3424 4559 5240 5431 826 Size: 219 1712 599 68 1135 681 191 1471 Fragments arranged by size: 1712 1471 1135 681 599 219 191 68 SfoI GGC'GCC Cuts at: 2263 2263 Size: 6076 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6076 SmaI CCC'GGG Cuts at: 1872 3140 3200 3403 1872 Size: 1268 60 203 4545 Fragments arranged by size: 4545 1268 203 60 SmlI C'TyrA_G Cuts at: 820 1017 1943 2327 3212 4180 5081 5343 820 Size: 197 926 384 885 968 901 262 1553 Fragments arranged by size: 1553 968 926 901 885 384 262 197 SnaBI TAC'GTA Cuts at: 493 3867 493 Size: 3374 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6076 SphI G_CATG'C Cuts at: 1132 3434 1132 Size: 2302 3774 SrfI GCCC'GGGC Cuts at: 3140 3140 Size: 6076 SspI AAT'ATT Cuts at: 5 52 4000 5 Size: 47 3948 2081 Fragments arranged by size: 3948 2081 47 StuI AGG'CCT Cuts at: 1517 2949 1517 Size: 1432 4644 StyI C'CwwG_G Cuts at: 513 1065 2257 2265 2382 2412 2487 2782 3003 Size: 552 1192 8 117 30 75 295 221 Cuts at: 3003 3051 3515 513 Size: 48 464 3074 Fragments arranged by size: 3074 1192 552 464 295 221 117 75 48 30 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2567 2805 4103 4120 4273 4458 4903 Size: 1255 987 238 1298 17 153 185 445 Cuts at: 4903 325 Size: 1498 Fragments arranged by size: 1498 1298 1255 987 445 238 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4322 6034 96 Size: 274 80 33 51 1443 2345 1712 138 Fragments arranged by size: 2345 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2542 2574 6036 6068 2542 Size: 32 3462 32 2550 Fragments arranged by size: 3462 2550 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6076 XbaI T'CTAG_A Cuts at: 3412 3412 Size: 6076 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2780 2389 Size: 391 5685 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6076 XmaI C'CCGG_G Cuts at: 1870 3138 3198 3401 1870 Size: 1268 60 203 4545 Fragments arranged by size: 4545 1268 203 60 XmnI GAAnn'nnTTC Cuts at: 4205 4205 Size: 6076 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3884 276 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SwaI