(Circular) (Six-base) MAPSORT of: pFN21AE4580.seq Check: 1486 from: 1 to: 7105 pFN21AE4580 7105 bp With 182 enzymes: SgfI * March 28, 2012 14:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3457 3457 Size: 7105 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4915 278 Size: 53 83 186 936 3379 2468 Fragments arranged by size: 3379 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4448 1937 Size: 2511 4594 Acc65I G'GTAC_C Cuts at: 4426 4426 Size: 7105 AclI AA'CG_TT Cuts at: 1563 5232 5605 1563 Size: 3669 373 3063 Fragments arranged by size: 3669 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4292 5168 6552 6820 1632 Size: 2660 876 1384 268 1917 Fragments arranged by size: 2660 1917 1384 876 268 AfeI AGC'GCT Cuts at: 3773 3773 Size: 7105 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6908 AflIII A'CryG_T Cuts at: 1203 6004 6981 1203 Size: 4801 977 1327 Fragments arranged by size: 4801 1327 977 AgeI A'CCGG_T Cuts at: 5936 5936 Size: 7105 AhdI GACnn_n'nnGTC Cuts at: 5834 5834 Size: 7105 AleI CACnn'nnGTG Cuts at: 1166 2127 2711 3404 1166 Size: 961 584 693 4867 Fragments arranged by size: 4867 961 693 584 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7105 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2708 2740 2708 Size: 32 7073 AlwNI CAG_nnn'CTG Cuts at: 2746 3139 3385 3472 3556 4848 6420 2746 Size: 393 246 87 84 1292 1572 3431 Fragments arranged by size: 3431 1572 1292 393 246 87 84 ApaI G_GGCC'C Cuts at: 3156 3156 Size: 7105 ApaLI G'TGCA_C Cuts at: 5162 6318 5162 Size: 1156 5949 ApoI r'AATT_y Cuts at: 1493 4414 4640 4792 6901 1493 Size: 2921 226 152 2109 1697 Fragments arranged by size: 2921 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5659 160 Size: 872 4627 1606 Fragments arranged by size: 4627 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7105 AvaI C'yCGr_G Cuts at: 1870 1943 2757 3281 3402 3638 3981 4430 1870 Size: 73 814 524 121 236 343 449 4545 Fragments arranged by size: 4545 814 524 449 343 236 121 73 AvrII C'CTAG_G Cuts at: 2572 2572 Size: 7105 BamHI G'GATC_C Cuts at: 3634 4435 4802 3634 Size: 801 367 5937 Fragments arranged by size: 5937 801 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2075 2097 2438 4304 Size: 325 217 627 279 9 22 341 1866 Cuts at: 4304 4426 5881 618 Size: 122 1455 1842 Fragments arranged by size: 1866 1842 1455 627 341 325 279 217 122 22 9 BanII G_rGCy'C Cuts at: 1391 2268 2454 3156 3448 3747 4217 4286 4424 Size: 877 186 702 292 299 470 69 138 Cuts at: 4424 1391 Size: 4072 Fragments arranged by size: 4072 877 702 470 299 292 186 138 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4235 4349 928 Size: 971 2336 114 3684 Fragments arranged by size: 3684 2336 971 114 BbvCI CC'TCA_GC Cuts at: 2220 3378 4012 2220 Size: 1158 634 5313 Fragments arranged by size: 5313 1158 634 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2300 2334 2975 3009 5296 5330 1903 Size: 34 363 34 641 34 2287 34 3678 Fragments arranged by size: 3678 2287 641 363 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4997 6207 4997 Size: 1210 5895 BclI T'GATC_A Cuts at: 1548 1548 Size: 7105 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2095 3615 5716 Size: 107 122 71 631 605 423 1520 2101 Cuts at: 5716 136 Size: 1525 Fragments arranged by size: 2101 1525 1520 631 605 423 122 107 71 BglII A'GATC_T Cuts at: 7100 7100 Size: 7105 BlpI GC'TnA_GC Cuts at: 3775 4361 4522 3775 Size: 586 161 6358 Fragments arranged by size: 6358 586 161 Bme1580I G_kGCm'C Cuts at: 1790 2441 2758 3156 4307 5166 6322 1790 Size: 651 317 398 1151 859 1156 2573 Fragments arranged by size: 2573 1156 1151 859 651 398 317 BmgBI CAC'GTC Cuts at: 1318 2866 3672 1318 Size: 1548 806 4751 Fragments arranged by size: 4751 1548 806 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4836 5794 442 Size: 416 53 83 399 24 3419 958 1753 Fragments arranged by size: 3419 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7105 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3790 3790 Size: 7105 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2696 3119 3494 3515 3533 3620 3841 3977 Size: 1457 423 375 21 18 87 221 136 Cuts at: 3977 4270 4304 5765 1239 Size: 293 34 1461 2579 Fragments arranged by size: 2579 1461 1457 423 375 293 221 136 87 34 21 18 Bpu10I CC'TnA_GC Cuts at: 2220 2338 3140 3378 4012 4216 2220 Size: 118 802 238 634 204 5109 Fragments arranged by size: 5109 802 634 238 204 118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2816 5230 6095 6393 2816 Size: 2414 865 298 3528 Fragments arranged by size: 3528 2414 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3953 5768 882 Size: 617 2454 1815 2219 Fragments arranged by size: 2454 2219 1815 617 BsaAI yAC'GTr Cuts at: 493 1204 4141 4896 493 Size: 711 2937 755 2702 Fragments arranged by size: 2937 2702 755 711 BsaBI GATnn'nnATC Cuts at: 3633 4043 7099 3633 Size: 410 3056 3639 Fragments arranged by size: 3639 3056 410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3523 4912 5294 5929 Size: 53 83 186 936 1990 1389 382 635 Cuts at: 5929 275 Size: 1451 Fragments arranged by size: 1990 1451 1389 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2124 5538 5936 6210 6357 6768 2124 Size: 3414 398 274 147 411 2461 Fragments arranged by size: 3414 2461 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2708 2738 3773 3803 1178 Size: 30 165 30 1305 30 1035 30 4480 Fragments arranged by size: 4480 1305 1035 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2341 2381 2462 2513 2635 2693 2732 2735 Size: 1155 40 81 51 122 58 39 3 Cuts at: 2735 2870 2873 3116 3200 3371 3374 3446 3491 Size: 135 3 243 84 171 3 72 45 Cuts at: 3491 3638 3746 3755 3782 3821 3857 4274 1186 Size: 147 108 9 27 39 36 417 4017 Fragments arranged by size: 4017 1155 417 243 171 147 135 122 108 84 81 72 58 51 45 40 39 39 36 27 9 3 3 3 BseYI C'CCAG_C Cuts at: 2554 3618 6308 6804 2554 Size: 1064 2690 496 2855 Fragments arranged by size: 2855 2690 1064 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3989 1163 Size: 573 2253 4279 Fragments arranged by size: 4279 2253 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 2064 3903 4555 5316 6344 Size: 275 186 45 18 1839 652 761 1028 Cuts at: 6344 1540 Size: 2301 Fragments arranged by size: 2301 1839 1028 761 652 275 186 45 18 BsiHKAI G_wGCw'C Cuts at: 3146 3448 3747 3827 4286 4362 5166 5251 6322 Size: 302 299 80 459 76 804 85 1071 Cuts at: 6322 3146 Size: 3929 Fragments arranged by size: 3929 1071 804 459 302 299 85 80 76 BsmI GAATG_Cn' Cuts at: 4616 4709 4616 Size: 93 7012 BsmBI CGTCTCn'nnnn_ Cuts at: 4142 4142 Size: 7105 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2441 2454 2758 3146 3156 3448 Size: 399 478 173 13 304 388 10 292 Cuts at: 3448 3747 3827 4217 4286 4307 4362 4424 5166 Size: 299 80 390 69 21 55 62 742 Cuts at: 5166 5251 6322 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 399 390 388 304 299 292 173 85 80 69 62 55 21 13 10 BspEI T'CCGG_A Cuts at: 6768 6768 Size: 7105 BspHI T'CATG_A Cuts at: 4994 6724 4994 Size: 1730 5375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2772 2952 3457 4460 844 Size: 1928 180 505 1003 3489 Fragments arranged by size: 3489 1928 1003 505 180 BsrBI CCG'CTC Cuts at: 1394 1823 2199 2275 2899 3037 3091 3643 4237 Size: 429 376 76 624 138 54 552 594 Cuts at: 4237 4992 1394 Size: 755 3507 Fragments arranged by size: 3507 755 624 594 552 429 376 138 76 54 BsrDI GCAATG_nn' Cuts at: 65 1248 5600 5774 65 Size: 1183 4352 174 1396 Fragments arranged by size: 4352 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2063 2625 3525 5749 5936 1700 Size: 110 253 562 900 2224 187 2869 Fragments arranged by size: 2869 2224 900 562 253 187 110 BsrGI T'GTAC_A Cuts at: 96 2748 96 Size: 2652 4453 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7105 BssSI C'ACGA_G Cuts at: 5165 6177 5165 Size: 1012 6093 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7105 BstBI TT'CG_AA Cuts at: 5909 5909 Size: 7105 BstEII G'GTnAC_C Cuts at: 1184 3193 1184 Size: 2009 5096 BstXI CCAn_nnnn'nTGG Cuts at: 2674 2696 3886 2674 Size: 22 1190 5893 Fragments arranged by size: 5893 1190 22 BstYI r'GATC_y Cuts at: 1972 3105 3634 3699 4435 4802 5187 5204 6656 Size: 1133 529 65 736 367 385 17 1452 Cuts at: 6656 6742 6754 7100 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1133 736 529 385 367 346 86 65 17 12 Bsu36I CC'TnA_GG Cuts at: 3183 3183 Size: 7105 BtgI C'CryG_G Cuts at: 513 1065 2004 2093 2778 4204 4308 513 Size: 552 939 89 685 1426 104 3310 Fragments arranged by size: 3310 1426 939 685 552 104 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2009 2313 2358 487 Size: 683 56 783 304 45 5234 Fragments arranged by size: 5234 783 683 304 56 45 BtsI GCAGTG_nn' Cuts at: 2143 2601 4621 5415 5435 2143 Size: 458 2020 794 20 3813 Fragments arranged by size: 3813 2020 794 458 20 ClaI AT'CG_AT Cuts at: 1554 4047 1554 Size: 2493 4612 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7070 DraI TTT'AAA Cuts at: 3202 4409 3202 Size: 1207 5898 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6112 809 Size: 507 4796 1802 Fragments arranged by size: 4796 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2061 3038 Size: 54 1425 50 275 18 213 18 977 Cuts at: 3038 4041 4552 5441 7070 8 Size: 1003 511 889 1629 43 Fragments arranged by size: 1629 1425 1003 977 889 511 275 213 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2043 2061 4552 1537 Size: 275 231 18 2491 4090 Fragments arranged by size: 4090 2491 275 231 18 EarI CTCTTCn'nnn_ Cuts at: 1353 2654 3015 5035 1353 Size: 1301 361 2020 3423 Fragments arranged by size: 3423 2020 1301 361 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3503 4255 5692 6066 6212 387 Size: 1064 138 1914 752 1437 374 146 1280 Fragments arranged by size: 1914 1437 1280 1064 752 374 146 138 EcoICRI GAG'CTC Cuts at: 3446 3745 4284 3446 Size: 299 539 6267 Fragments arranged by size: 6267 539 299 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2696 3119 3494 3515 3533 3620 3841 Size: 393 1064 423 375 21 18 87 221 Cuts at: 3841 3977 4270 4292 4304 5168 5765 6552 6820 Size: 136 293 22 12 864 597 787 268 Cuts at: 6820 1239 Size: 1524 Fragments arranged by size: 1524 1064 864 787 597 423 393 375 293 268 221 136 87 22 21 18 12 EcoNI CCTnn'n_nnAGG Cuts at: 2576 3839 3890 4319 2576 Size: 1263 51 429 5362 Fragments arranged by size: 5362 1263 429 51 EcoO109I rG'GnC_Cy Cuts at: 2647 2743 2761 2770 3152 3153 3459 3991 2647 Size: 96 18 9 382 1 306 532 5761 Fragments arranged by size: 5761 532 382 306 96 18 9 1 EcoRI G'AATT_C Cuts at: 4414 4792 4414 Size: 378 6727 FspI TGC'GCA Cuts at: 5611 5611 Size: 7105 HaeII r_GCGC'y Cuts at: 1678 2539 3775 4268 6252 1678 Size: 861 1236 493 1984 2531 Fragments arranged by size: 2531 1984 1236 861 493 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2336 2368 5746 5778 5820 Size: 32 135 32 764 32 3378 32 42 Cuts at: 5820 5852 1373 Size: 32 2626 Fragments arranged by size: 3378 2626 764 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4449 4695 669 Size: 988 281 2511 246 3079 Fragments arranged by size: 3079 2511 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7105 HpaI GTT'AAC Cuts at: 4695 4695 Size: 7105 KpnI G_GTAC'C Cuts at: 4430 4430 Size: 7105 MfeI C'AATT_G Cuts at: 4704 4704 Size: 7105 MluI A'CGCG_T Cuts at: 6981 6981 Size: 7105 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3372 6219 6403 1305 Size: 2067 2847 184 2007 Fragments arranged by size: 2847 2067 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4043 10 Size: 54 1425 343 2211 3072 Fragments arranged by size: 3072 2211 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2127 2711 3404 5063 5422 5581 518 Size: 648 961 584 693 1659 359 159 2042 Fragments arranged by size: 2042 1659 961 693 648 584 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 2318 2462 2522 2632 3511 3556 3613 Size: 489 223 144 60 110 879 45 57 Cuts at: 3613 3923 4189 4365 4371 4521 5198 6346 6591 Size: 310 266 176 6 150 677 1148 245 Cuts at: 6591 1606 Size: 2120 Fragments arranged by size: 2120 1148 879 677 489 310 266 245 223 176 150 144 110 60 57 45 6 NaeI GCC'GGC Cuts at: 2065 2627 3527 2065 Size: 562 900 5643 Fragments arranged by size: 5643 900 562 NcoI C'CATG_G Cuts at: 513 1065 2004 4204 513 Size: 552 939 2200 3414 Fragments arranged by size: 3414 2200 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7105 NgoMIV G'CCGG_C Cuts at: 2063 2625 3525 2063 Size: 562 900 5643 Fragments arranged by size: 5643 900 562 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7105 NotI GC'GGCC_GC Cuts at: 4552 4552 Size: 7105 NruI TCG'CGA Cuts at: 3729 3729 Size: 7105 NspI r_CATG'y Cuts at: 1132 1229 2789 4463 6008 1132 Size: 97 1560 1674 1545 2229 Fragments arranged by size: 2229 1674 1560 1545 97 PasI CC'CwG_GG Cuts at: 1348 4300 1348 Size: 2952 4153 PciI A'CATG_T Cuts at: 6004 6004 Size: 7105 PflMI CCAn_nnn'nTGG Cuts at: 1108 2554 1108 Size: 1446 5659 PfoI T'CCnGG_A Cuts at: 3885 4320 3885 Size: 435 6670 PmeI GTTT'AAAC Cuts at: 4409 4409 Size: 7105 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2708 2740 5174 5206 6687 6719 2708 Size: 32 2434 32 1481 32 3094 Fragments arranged by size: 3094 2434 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2647 2743 2761 3459 2647 Size: 96 18 698 6293 Fragments arranged by size: 6293 698 96 18 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7105 PsiI TTA'TAA Cuts at: 4675 4675 Size: 7105 PspOMI G'GGCC_C Cuts at: 3152 3152 Size: 7105 PstI C_TGCA'G Cuts at: 830 2608 2637 2769 2853 3129 3504 4457 830 Size: 1778 29 132 84 276 375 953 3478 Fragments arranged by size: 3478 1778 953 375 276 132 84 29 PvuI CG_AT'CG Cuts at: 2001 3903 2001 Size: 1902 5203 PvuII CAG'CTG Cuts at: 2462 2522 2632 3511 3556 3923 4189 2462 Size: 60 110 879 45 367 266 5378 Fragments arranged by size: 5378 879 367 266 110 60 45 SacI G_AGCT'C Cuts at: 3448 3747 4286 3448 Size: 299 539 6267 Fragments arranged by size: 6267 539 299 SacII CC_GC'GG Cuts at: 2096 2096 Size: 7105 SalI G'TCGA_C Cuts at: 1936 4447 1936 Size: 2511 4594 SanDI GG'GwC_CC Cuts at: 3459 3459 Size: 7105 SapI GCTCTTCn'nnn_ Cuts at: 3015 3015 Size: 7105 SbfI CC_TGCA'GG Cuts at: 2769 4457 2769 Size: 1688 5417 ScaI AGT'ACT Cuts at: 5353 7065 5353 Size: 1712 5393 SexAI A'CCwGG_T Cuts at: 2883 2883 Size: 7105 SfcI C'TryA_G Cuts at: 826 1045 2604 2633 2765 2849 3125 3500 3548 Size: 219 1559 29 132 84 276 375 48 Cuts at: 3548 4453 5588 6269 6460 826 Size: 905 1135 681 191 1471 Fragments arranged by size: 1559 1471 1135 905 681 375 276 219 191 132 84 48 29 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7105 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7105 SmaI CCC'GGG Cuts at: 1872 2759 3983 4432 1872 Size: 887 1224 449 4545 Fragments arranged by size: 4545 1224 887 449 SmlI C'TyrA_G Cuts at: 820 1017 1943 2831 5209 6110 6372 820 Size: 197 926 888 2378 901 262 1553 Fragments arranged by size: 2378 1553 926 901 888 262 197 SnaBI TAC'GTA Cuts at: 493 4896 493 Size: 4403 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7105 SphI G_CATG'C Cuts at: 1132 2789 4463 1132 Size: 1657 1674 3774 Fragments arranged by size: 3774 1674 1657 SspI AAT'ATT Cuts at: 5 52 5029 5 Size: 47 4977 2081 Fragments arranged by size: 4977 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7105 StyI C'CwwG_G Cuts at: 513 1065 2004 2406 2442 2526 2572 3690 4170 Size: 552 939 402 36 84 46 1118 480 Cuts at: 4170 4204 4401 4544 513 Size: 34 197 143 3074 Fragments arranged by size: 3074 1118 939 552 480 402 197 143 84 46 36 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4183 5132 5149 5302 5487 5932 325 Size: 1255 2603 949 17 153 185 445 1498 Fragments arranged by size: 2603 1498 1255 949 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2748 4020 4056 Size: 274 80 33 51 1443 771 1272 36 Cuts at: 4056 5351 7063 96 Size: 1295 1712 138 Fragments arranged by size: 1712 1443 1295 1272 771 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3033 3065 7065 7097 3033 Size: 32 4000 32 3041 Fragments arranged by size: 4000 3041 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3330 1316 Size: 2014 5091 XbaI T'CTAG_A Cuts at: 4441 4441 Size: 7105 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3296 3712 3883 3296 Size: 416 171 6518 Fragments arranged by size: 6518 416 171 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7105 XmaI C'CCGG_G Cuts at: 1870 2757 3981 4430 1870 Size: 887 1224 449 4545 Fragments arranged by size: 4545 1224 887 449 XmnI GAAnn'nnTTC Cuts at: 5234 5234 Size: 7105 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4913 276 Size: 53 83 186 936 3379 2468 Fragments arranged by size: 3379 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NsiI PacI PmlI PspXI PsrI RsrII SfiI SfoI SrfI SwaI