(Linear) (Six-base) MAPSORT of: KIEE4580.seq Check: 6441 from: 1 to: 2400 KIEE4580.orf With 182 enzymes: SgfI * March 22, 2012 18:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1452 2400 Size: 1452 948 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2287 2400 Size: 2287 113 AfeI AGC'GCT Cuts at: 0 1768 2400 Size: 1768 632 AleI CACnn'nnGTG Cuts at: 0 122 706 1399 2400 Size: 122 584 693 1001 Fragments arranged by size: 1001 693 584 122 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 703 735 2400 Size: 703 32 1665 Fragments arranged by size: 1665 703 32 AlwNI CAG_nnn'CTG Cuts at: 0 741 1134 1380 1467 1551 2400 Size: 741 393 246 87 84 849 Fragments arranged by size: 849 741 393 246 87 84 ApaI G_GGCC'C Cuts at: 0 1151 2400 Size: 1151 1249 AvaI C'yCGr_G Cuts at: 0 752 1276 1397 1633 1976 2400 Size: 752 524 121 236 343 424 Fragments arranged by size: 752 524 424 343 236 121 AvrII C'CTAG_G Cuts at: 0 567 2400 Size: 567 1833 BamHI G'GATC_C Cuts at: 0 1629 2400 Size: 1629 771 BanI G'GyrC_C Cuts at: 0 61 70 92 433 2299 2400 Size: 61 9 22 341 1866 101 Fragments arranged by size: 1866 341 101 61 22 9 BanII G_rGCy'C Cuts at: 0 263 449 1151 1443 1742 2212 2281 2400 Size: 263 186 702 292 299 470 69 119 Fragments arranged by size: 702 470 299 292 263 186 119 69 BbsI GAAGACnn'nnnn_ Cuts at: 0 2230 2344 2400 Size: 2230 114 56 Fragments arranged by size: 2230 114 56 BbvCI CC'TCA_GC Cuts at: 0 215 1373 2007 2400 Size: 215 1158 634 393 Fragments arranged by size: 1158 634 393 215 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 295 329 970 1004 2400 Size: 295 34 641 34 1396 Fragments arranged by size: 1396 641 295 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 90 1610 2400 Size: 90 1520 790 Fragments arranged by size: 1520 790 90 BlpI GC'TnA_GC Cuts at: 0 1770 2356 2400 Size: 1770 586 44 Fragments arranged by size: 1770 586 44 Bme1580I G_kGCm'C Cuts at: 0 436 753 1151 2302 2400 Size: 436 317 398 1151 98 Fragments arranged by size: 1151 436 398 317 98 BmgBI CAC'GTC Cuts at: 0 861 1667 2400 Size: 861 806 733 Fragments arranged by size: 861 806 733 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1785 2400 Size: 1785 615 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 691 1114 1489 1510 1528 1615 1836 1972 Size: 691 423 375 21 18 87 221 136 Cuts at: 1972 2265 2299 2400 Size: 293 34 101 Fragments arranged by size: 691 423 375 293 221 136 101 87 34 21 18 Bpu10I CC'TnA_GC Cuts at: 0 215 333 1135 1373 2007 2211 2400 Size: 215 118 802 238 634 204 189 Fragments arranged by size: 802 634 238 215 204 189 118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 811 2400 Size: 811 1589 BsaI GGTCTCn'nnnn_ Cuts at: 0 1948 2400 Size: 1948 452 BsaAI yAC'GTr Cuts at: 0 2136 2400 Size: 2136 264 BsaBI GATnn'nnATC Cuts at: 0 1628 2038 2400 Size: 1628 410 362 Fragments arranged by size: 1628 410 362 BsaHI Gr'CG_yC Cuts at: 0 1518 2400 Size: 1518 882 BsaWI w'CCGG_w Cuts at: 0 119 2400 Size: 119 2281 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 703 733 1768 1798 2400 Size: 703 30 1035 30 602 Fragments arranged by size: 1035 703 602 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 336 376 457 508 630 688 727 730 Size: 336 40 81 51 122 58 39 3 Cuts at: 730 865 868 1111 1195 1366 1369 1441 1486 Size: 135 3 243 84 171 3 72 45 Cuts at: 1486 1633 1741 1750 1777 1816 1852 2269 2400 Size: 147 108 9 27 39 36 417 131 Fragments arranged by size: 417 336 243 171 147 135 131 122 108 84 81 72 58 51 45 40 39 39 36 27 9 3 3 3 BseYI C'CCAG_C Cuts at: 0 549 1613 2400 Size: 549 1064 787 Fragments arranged by size: 1064 787 549 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1984 2400 Size: 1984 416 BsiEI CG_ry'CG Cuts at: 0 41 59 1898 2400 Size: 41 18 1839 502 Fragments arranged by size: 1839 502 41 18 BsiHKAI G_wGCw'C Cuts at: 0 1141 1443 1742 1822 2281 2357 2400 Size: 1141 302 299 80 459 76 43 Fragments arranged by size: 1141 459 302 299 80 76 43 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2137 2400 Size: 2137 263 Bsp1286I G_dGCh'C Cuts at: 0 263 436 449 753 1141 1151 1443 1742 Size: 263 173 13 304 388 10 292 299 Cuts at: 1742 1822 2212 2281 2302 2357 2400 Size: 80 390 69 21 55 43 Fragments arranged by size: 390 388 304 299 292 263 173 80 69 55 43 21 13 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 767 947 1452 2400 Size: 767 180 505 948 Fragments arranged by size: 948 767 505 180 BsrBI CCG'CTC Cuts at: 0 194 270 894 1032 1086 1638 2232 2400 Size: 194 76 624 138 54 552 594 168 Fragments arranged by size: 624 594 552 194 168 138 76 54 BsrFI r'CCGG_y Cuts at: 0 58 620 1520 2400 Size: 58 562 900 880 Fragments arranged by size: 900 880 562 58 BsrGI T'GTAC_A Cuts at: 0 743 2400 Size: 743 1657 BstEII G'GTnAC_C Cuts at: 0 1188 2400 Size: 1188 1212 BstXI CCAn_nnnn'nTGG Cuts at: 0 669 691 1881 2400 Size: 669 22 1190 519 Fragments arranged by size: 1190 669 519 22 BstYI r'GATC_y Cuts at: 0 1100 1629 1694 2400 Size: 1100 529 65 706 Fragments arranged by size: 1100 706 529 65 Bsu36I CC'TnA_GG Cuts at: 0 1178 2400 Size: 1178 1222 BtgI C'CryG_G Cuts at: 0 88 773 2199 2303 2400 Size: 88 685 1426 104 97 Fragments arranged by size: 1426 685 104 97 88 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 4 308 353 2400 Size: 4 304 45 2047 Fragments arranged by size: 2047 304 45 4 BtsI GCAGTG_nn' Cuts at: 0 138 596 2400 Size: 138 458 1804 Fragments arranged by size: 1804 458 138 ClaI AT'CG_AT Cuts at: 0 2042 2400 Size: 2042 358 DraI TTT'AAA Cuts at: 0 1197 2400 Size: 1197 1203 EaeI y'GGCC_r Cuts at: 0 38 56 1033 2036 2400 Size: 38 18 977 1003 364 Fragments arranged by size: 1003 977 364 38 18 EagI C'GGCC_G Cuts at: 0 38 56 2400 Size: 38 18 2344 Fragments arranged by size: 2344 38 18 EarI CTCTTCn'nnn_ Cuts at: 0 649 1010 2400 Size: 649 361 1390 Fragments arranged by size: 1390 649 361 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1498 2250 2400 Size: 1498 752 150 Fragments arranged by size: 1498 752 150 EcoICRI GAG'CTC Cuts at: 0 1441 1740 2279 2400 Size: 1441 299 539 121 Fragments arranged by size: 1441 539 299 121 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 691 1114 1489 1510 1528 1615 1836 1972 Size: 691 423 375 21 18 87 221 136 Cuts at: 1972 2265 2287 2299 2400 Size: 293 22 12 101 Fragments arranged by size: 691 423 375 293 221 136 101 87 22 21 18 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 571 1834 1885 2314 2400 Size: 571 1263 51 429 86 Fragments arranged by size: 1263 571 429 86 51 EcoO109I rG'GnC_Cy Cuts at: 0 642 738 756 765 1147 1148 1454 1986 Size: 642 96 18 9 382 1 306 532 Cuts at: 1986 2400 Size: 414 Fragments arranged by size: 642 532 414 382 306 96 18 9 1 HaeII r_GCGC'y Cuts at: 0 534 1770 2263 2400 Size: 534 1236 493 137 Fragments arranged by size: 1236 534 493 137 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 331 363 2400 Size: 331 32 2037 Fragments arranged by size: 2037 331 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1367 2400 Size: 1367 1033 MscI TGG'CCA Cuts at: 0 2038 2400 Size: 2038 362 MslI CAynn'nnrTG Cuts at: 0 122 706 1399 2400 Size: 122 584 693 1001 Fragments arranged by size: 1001 693 584 122 MspA1I CmG'CkG Cuts at: 0 90 313 457 517 627 1506 1551 1608 Size: 90 223 144 60 110 879 45 57 Cuts at: 1608 1918 2184 2360 2366 2400 Size: 310 266 176 6 34 Fragments arranged by size: 879 310 266 223 176 144 110 90 60 57 45 34 6 NaeI GCC'GGC Cuts at: 0 60 622 1522 2400 Size: 60 562 900 878 Fragments arranged by size: 900 878 562 60 NcoI C'CATG_G Cuts at: 0 2199 2400 Size: 2199 201 NgoMIV G'CCGG_C Cuts at: 0 58 620 1520 2400 Size: 58 562 900 880 Fragments arranged by size: 900 880 562 58 NruI TCG'CGA Cuts at: 0 1724 2400 Size: 1724 676 NspI r_CATG'y Cuts at: 0 784 2400 Size: 784 1616 PasI CC'CwG_GG Cuts at: 0 2295 2400 Size: 2295 105 PflMI CCAn_nnn'nTGG Cuts at: 0 549 2400 Size: 549 1851 PfoI T'CCnGG_A Cuts at: 0 1880 2315 2400 Size: 1880 435 85 Fragments arranged by size: 1880 435 85 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 703 735 2400 Size: 703 32 1665 Fragments arranged by size: 1665 703 32 PpuMI rG'GwC_Cy Cuts at: 0 642 738 756 1454 2400 Size: 642 96 18 698 946 Fragments arranged by size: 946 698 642 96 18 PspOMI G'GGCC_C Cuts at: 0 1147 2400 Size: 1147 1253 PstI C_TGCA'G Cuts at: 0 603 632 764 848 1124 1499 2400 Size: 603 29 132 84 276 375 901 Fragments arranged by size: 901 603 375 276 132 84 29 PvuI CG_AT'CG Cuts at: 0 1898 2400 Size: 1898 502 PvuII CAG'CTG Cuts at: 0 457 517 627 1506 1551 1918 2184 2400 Size: 457 60 110 879 45 367 266 216 Fragments arranged by size: 879 457 367 266 216 110 60 45 SacI G_AGCT'C Cuts at: 0 1443 1742 2281 2400 Size: 1443 299 539 119 Fragments arranged by size: 1443 539 299 119 SacII CC_GC'GG Cuts at: 0 91 2400 Size: 91 2309 SanDI GG'GwC_CC Cuts at: 0 1454 2400 Size: 1454 946 SapI GCTCTTCn'nnn_ Cuts at: 0 1010 2400 Size: 1010 1390 SbfI CC_TGCA'GG Cuts at: 0 764 2400 Size: 764 1636 SexAI A'CCwGG_T Cuts at: 0 878 2400 Size: 878 1522 SfcI C'TryA_G Cuts at: 0 599 628 760 844 1120 1495 1543 2400 Size: 599 29 132 84 276 375 48 857 Fragments arranged by size: 857 599 375 276 132 84 48 29 SmaI CCC'GGG Cuts at: 0 754 1978 2400 Size: 754 1224 422 Fragments arranged by size: 1224 754 422 SmlI C'TyrA_G Cuts at: 0 826 2400 Size: 826 1574 SphI G_CATG'C Cuts at: 0 784 2400 Size: 784 1616 StyI C'CwwG_G Cuts at: 0 401 437 521 567 1685 2165 2199 2400 Size: 401 36 84 46 1118 480 34 201 Fragments arranged by size: 1118 480 401 201 84 46 36 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2178 2400 Size: 2178 222 TatI w'GTAC_w Cuts at: 0 743 2015 2051 2400 Size: 743 1272 36 349 Fragments arranged by size: 1272 743 349 36 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1028 1060 2400 Size: 1028 32 1340 Fragments arranged by size: 1340 1028 32 Tth111I GACn'n_nGTC Cuts at: 0 1325 2400 Size: 1325 1075 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1291 1707 1878 2400 Size: 1291 416 171 522 Fragments arranged by size: 1291 522 416 171 XmaI C'CCGG_G Cuts at: 0 752 1976 2400 Size: 752 1224 424 Fragments arranged by size: 1224 752 424 Enzymes that do cut: AarI AcuI AfeI AleI AloI AlwNI ApaI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NruI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuI PvuII SacI SacII SanDI SapI SbfI SexAI SfcI SmaI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI BaeI BbeI BciVI BclI BfrBI BglII BmrI BmtI BsiWI BsmI BspEI BspHI BsrDI BssHII BssSI BstAPI BstBI BstZ17I CspCI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI RsrII SalI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI