Restriction map for pFN21AE4580
 (Linear) (Six-base) MAPSORT of: KIEE4580.seq  Check: 6441  from: 1  to: 2400



KIEE4580.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1452    2400 
   Size:       1452     948
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2287    2400 
   Size:       2287     113
 
AfeI AGC'GCT

Cuts at:      0    1768    2400 
   Size:       1768     632
 
AleI CACnn'nnGTG

Cuts at:      0     122     706    1399    2400 
   Size:        122     584     693    1001

  Fragments arranged by size: 

                1001     693     584     122
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     703     735    2400 
   Size:        703      32    1665

  Fragments arranged by size: 

                1665     703      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     741    1134    1380    1467    1551    2400 
   Size:        741     393     246      87      84     849

  Fragments arranged by size: 

                 849     741     393     246      87      84
 
ApaI G_GGCC'C

Cuts at:      0    1151    2400 
   Size:       1151    1249
 
AvaI C'yCGr_G

Cuts at:      0     752    1276    1397    1633    1976    2400 
   Size:        752     524     121     236     343     424

  Fragments arranged by size: 

                 752     524     424     343     236     121
 
AvrII C'CTAG_G

Cuts at:      0     567    2400 
   Size:        567    1833
 
BamHI G'GATC_C

Cuts at:      0    1629    2400 
   Size:       1629     771
 
BanI G'GyrC_C

Cuts at:      0      61      70      92     433    2299    2400 
   Size:         61       9      22     341    1866     101

  Fragments arranged by size: 

                1866     341     101      61      22       9
 
BanII G_rGCy'C

Cuts at:      0     263     449    1151    1443    1742    2212    2281    2400 
   Size:        263     186     702     292     299     470      69     119

  Fragments arranged by size: 

                 702     470     299     292     263     186     119      69
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2230    2344    2400 
   Size:       2230     114      56

  Fragments arranged by size: 

                2230     114      56
 
BbvCI CC'TCA_GC

Cuts at:      0     215    1373    2007    2400 
   Size:        215    1158     634     393

  Fragments arranged by size: 

                1158     634     393     215
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     295     329     970    1004    2400 
   Size:        295      34     641      34    1396

  Fragments arranged by size: 

                1396     641     295      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      90    1610    2400 
   Size:         90    1520     790

  Fragments arranged by size: 

                1520     790      90
 
BlpI GC'TnA_GC

Cuts at:      0    1770    2356    2400 
   Size:       1770     586      44

  Fragments arranged by size: 

                1770     586      44
 
Bme1580I G_kGCm'C

Cuts at:      0     436     753    1151    2302    2400 
   Size:        436     317     398    1151      98

  Fragments arranged by size: 

                1151     436     398     317      98
 
BmgBI CAC'GTC

Cuts at:      0     861    1667    2400 
   Size:        861     806     733

  Fragments arranged by size: 

                 861     806     733
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1785    2400 
   Size:       1785     615
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691    1114    1489    1510    1528    1615    1836    1972 
   Size:        691     423     375      21      18      87     221     136

Cuts at:   1972    2265    2299    2400 
   Size:        293      34     101

  Fragments arranged by size: 

                 691     423     375     293     221     136     101      87
                  34      21      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     215     333    1135    1373    2007    2211    2400 
   Size:        215     118     802     238     634     204     189

  Fragments arranged by size: 

                 802     634     238     215     204     189     118
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811    2400 
   Size:        811    1589
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1948    2400 
   Size:       1948     452
 
BsaAI yAC'GTr

Cuts at:      0    2136    2400 
   Size:       2136     264
 
BsaBI GATnn'nnATC

Cuts at:      0    1628    2038    2400 
   Size:       1628     410     362

  Fragments arranged by size: 

                1628     410     362
 
BsaHI Gr'CG_yC

Cuts at:      0    1518    2400 
   Size:       1518     882
 
BsaWI w'CCGG_w

Cuts at:      0     119    2400 
   Size:        119    2281
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     703     733    1768    1798    2400 
   Size:        703      30    1035      30     602

  Fragments arranged by size: 

                1035     703     602      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     336     376     457     508     630     688     727     730 
   Size:        336      40      81      51     122      58      39       3

Cuts at:    730     865     868    1111    1195    1366    1369    1441    1486 
   Size:        135       3     243      84     171       3      72      45

Cuts at:   1486    1633    1741    1750    1777    1816    1852    2269    2400 
   Size:        147     108       9      27      39      36     417     131

  Fragments arranged by size: 

                 417     336     243     171     147     135     131     122
                 108      84      81      72      58      51      45      40
                  39      39      36      27       9       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     549    1613    2400 
   Size:        549    1064     787

  Fragments arranged by size: 

                1064     787     549
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1984    2400 
   Size:       1984     416
 
BsiEI CG_ry'CG

Cuts at:      0      41      59    1898    2400 
   Size:         41      18    1839     502

  Fragments arranged by size: 

                1839     502      41      18
 
BsiHKAI G_wGCw'C

Cuts at:      0    1141    1443    1742    1822    2281    2357    2400 
   Size:       1141     302     299      80     459      76      43

  Fragments arranged by size: 

                1141     459     302     299      80      76      43
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2137    2400 
   Size:       2137     263
 
Bsp1286I G_dGCh'C

Cuts at:      0     263     436     449     753    1141    1151    1443    1742 
   Size:        263     173      13     304     388      10     292     299

Cuts at:   1742    1822    2212    2281    2302    2357    2400 
   Size:         80     390      69      21      55      43

  Fragments arranged by size: 

                 390     388     304     299     292     263     173      80
                  69      55      43      21      13      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     767     947    1452    2400 
   Size:        767     180     505     948

  Fragments arranged by size: 

                 948     767     505     180
 
BsrBI CCG'CTC

Cuts at:      0     194     270     894    1032    1086    1638    2232    2400 
   Size:        194      76     624     138      54     552     594     168

  Fragments arranged by size: 

                 624     594     552     194     168     138      76      54
            
 
BsrFI r'CCGG_y

Cuts at:      0      58     620    1520    2400 
   Size:         58     562     900     880

  Fragments arranged by size: 

                 900     880     562      58
 
BsrGI T'GTAC_A

Cuts at:      0     743    2400 
   Size:        743    1657
 
BstEII G'GTnAC_C

Cuts at:      0    1188    2400 
   Size:       1188    1212
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     669     691    1881    2400 
   Size:        669      22    1190     519

  Fragments arranged by size: 

                1190     669     519      22
 
BstYI r'GATC_y

Cuts at:      0    1100    1629    1694    2400 
   Size:       1100     529      65     706

  Fragments arranged by size: 

                1100     706     529      65
 
Bsu36I CC'TnA_GG

Cuts at:      0    1178    2400 
   Size:       1178    1222
 
BtgI C'CryG_G

Cuts at:      0      88     773    2199    2303    2400 
   Size:         88     685    1426     104      97

  Fragments arranged by size: 

                1426     685     104      97      88
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       4     308     353    2400 
   Size:          4     304      45    2047

  Fragments arranged by size: 

                2047     304      45       4
 
BtsI GCAGTG_nn'

Cuts at:      0     138     596    2400 
   Size:        138     458    1804

  Fragments arranged by size: 

                1804     458     138
 
ClaI AT'CG_AT

Cuts at:      0    2042    2400 
   Size:       2042     358
 
DraI TTT'AAA

Cuts at:      0    1197    2400 
   Size:       1197    1203
 
EaeI y'GGCC_r

Cuts at:      0      38      56    1033    2036    2400 
   Size:         38      18     977    1003     364

  Fragments arranged by size: 

                1003     977     364      38      18
 
EagI C'GGCC_G

Cuts at:      0      38      56    2400 
   Size:         38      18    2344

  Fragments arranged by size: 

                2344      38      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     649    1010    2400 
   Size:        649     361    1390

  Fragments arranged by size: 

                1390     649     361
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1498    2250    2400 
   Size:       1498     752     150

  Fragments arranged by size: 

                1498     752     150
 
EcoICRI GAG'CTC

Cuts at:      0    1441    1740    2279    2400 
   Size:       1441     299     539     121

  Fragments arranged by size: 

                1441     539     299     121
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691    1114    1489    1510    1528    1615    1836    1972 
   Size:        691     423     375      21      18      87     221     136

Cuts at:   1972    2265    2287    2299    2400 
   Size:        293      22      12     101

  Fragments arranged by size: 

                 691     423     375     293     221     136     101      87
                  22      21      18      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     571    1834    1885    2314    2400 
   Size:        571    1263      51     429      86

  Fragments arranged by size: 

                1263     571     429      86      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0     642     738     756     765    1147    1148    1454    1986 
   Size:        642      96      18       9     382       1     306     532

Cuts at:   1986    2400 
   Size:        414

  Fragments arranged by size: 

                 642     532     414     382     306      96      18       9
                   1
 
HaeII r_GCGC'y

Cuts at:      0     534    1770    2263    2400 
   Size:        534    1236     493     137

  Fragments arranged by size: 

                1236     534     493     137
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     331     363    2400 
   Size:        331      32    2037

  Fragments arranged by size: 

                2037     331      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1367    2400 
   Size:       1367    1033
 
MscI TGG'CCA

Cuts at:      0    2038    2400 
   Size:       2038     362
 
MslI CAynn'nnrTG

Cuts at:      0     122     706    1399    2400 
   Size:        122     584     693    1001

  Fragments arranged by size: 

                1001     693     584     122
 
MspA1I CmG'CkG

Cuts at:      0      90     313     457     517     627    1506    1551    1608 
   Size:         90     223     144      60     110     879      45      57

Cuts at:   1608    1918    2184    2360    2366    2400 
   Size:        310     266     176       6      34

  Fragments arranged by size: 

                 879     310     266     223     176     144     110      90
                  60      57      45      34       6
 
NaeI GCC'GGC

Cuts at:      0      60     622    1522    2400 
   Size:         60     562     900     878

  Fragments arranged by size: 

                 900     878     562      60
 
NcoI C'CATG_G

Cuts at:      0    2199    2400 
   Size:       2199     201
 
NgoMIV G'CCGG_C

Cuts at:      0      58     620    1520    2400 
   Size:         58     562     900     880

  Fragments arranged by size: 

                 900     880     562      58
 
NruI TCG'CGA

Cuts at:      0    1724    2400 
   Size:       1724     676
 
NspI r_CATG'y

Cuts at:      0     784    2400 
   Size:        784    1616
 
PasI CC'CwG_GG

Cuts at:      0    2295    2400 
   Size:       2295     105
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     549    2400 
   Size:        549    1851
 
PfoI T'CCnGG_A

Cuts at:      0    1880    2315    2400 
   Size:       1880     435      85

  Fragments arranged by size: 

                1880     435      85
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     703     735    2400 
   Size:        703      32    1665

  Fragments arranged by size: 

                1665     703      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     642     738     756    1454    2400 
   Size:        642      96      18     698     946

  Fragments arranged by size: 

                 946     698     642      96      18
 
PspOMI G'GGCC_C

Cuts at:      0    1147    2400 
   Size:       1147    1253
 
PstI C_TGCA'G

Cuts at:      0     603     632     764     848    1124    1499    2400 
   Size:        603      29     132      84     276     375     901

  Fragments arranged by size: 

                 901     603     375     276     132      84      29
 
PvuI CG_AT'CG

Cuts at:      0    1898    2400 
   Size:       1898     502
 
PvuII CAG'CTG

Cuts at:      0     457     517     627    1506    1551    1918    2184    2400 
   Size:        457      60     110     879      45     367     266     216

  Fragments arranged by size: 

                 879     457     367     266     216     110      60      45
            
 
SacI G_AGCT'C

Cuts at:      0    1443    1742    2281    2400 
   Size:       1443     299     539     119

  Fragments arranged by size: 

                1443     539     299     119
 
SacII CC_GC'GG

Cuts at:      0      91    2400 
   Size:         91    2309
 
SanDI GG'GwC_CC

Cuts at:      0    1454    2400 
   Size:       1454     946
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1010    2400 
   Size:       1010    1390
 
SbfI CC_TGCA'GG

Cuts at:      0     764    2400 
   Size:        764    1636
 
SexAI A'CCwGG_T

Cuts at:      0     878    2400 
   Size:        878    1522
 
SfcI C'TryA_G

Cuts at:      0     599     628     760     844    1120    1495    1543    2400 
   Size:        599      29     132      84     276     375      48     857

  Fragments arranged by size: 

                 857     599     375     276     132      84      48      29
            
 
SmaI CCC'GGG

Cuts at:      0     754    1978    2400 
   Size:        754    1224     422

  Fragments arranged by size: 

                1224     754     422
 
SmlI C'TyrA_G

Cuts at:      0     826    2400 
   Size:        826    1574
 
SphI G_CATG'C

Cuts at:      0     784    2400 
   Size:        784    1616
 
StyI C'CwwG_G

Cuts at:      0     401     437     521     567    1685    2165    2199    2400 
   Size:        401      36      84      46    1118     480      34     201

  Fragments arranged by size: 

                1118     480     401     201      84      46      36      34
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2178    2400 
   Size:       2178     222
 
TatI w'GTAC_w

Cuts at:      0     743    2015    2051    2400 
   Size:        743    1272      36     349

  Fragments arranged by size: 

                1272     743     349      36
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1028    1060    2400 
   Size:       1028      32    1340

  Fragments arranged by size: 

                1340    1028      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1325    2400 
   Size:       1325    1075
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1291    1707    1878    2400 
   Size:       1291     416     171     522

  Fragments arranged by size: 

                1291     522     416     171
 
XmaI C'CCGG_G

Cuts at:      0     752    1976    2400 
   Size:        752    1224     424

  Fragments arranged by size: 

                1224     752     424
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AleI     AloI    AlwNI     ApaI     AvaI
    AvrII    BamHI     BanI    BanII     BbsI    BbvCI     BcgI     BglI
     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NruI     NspI     PasI    PflMI     PfoI     PpiI
    PpuMI   PspOMI     PstI     PvuI    PvuII     SacI    SacII    SanDI
     SapI     SbfI    SexAI     SfcI     SmaI     SmlI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI     BbeI
    BciVI     BclI    BfrBI    BglII     BmrI     BmtI    BsiWI     BsmI
    BspEI    BspHI    BsrDI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
    CspCI   DraIII     DrdI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NdeI     NheI     NotI     NsiI     PacI     PciI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI    RsrII     SalI     ScaI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     XbaI     XhoI     XmnI     ZraI
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