################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AviII AvrII BamHI BanII BbeI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspEI BspLU11I BsrDI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT14I FseI FspI HaeII HincII HindII HindIII HpaI HphI KasI KspI MamI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SpeI SphI Sse8387I StuI StyI SwaI Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### Acc65I 1271 GGTACC G^GTACC AcyI 1052 GACGCC G[GA]^CG[CT]C AhdI 1122 GACGATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 558 GATCC GGATC(4/5) Asp718I 1271 GGTACC G^GTACC AspEI 1122 GACGATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 270 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 422 TGGCCA TGG^CCA BanI 1271 GGTACC G^G[CT][GA]CC BbrPI 528 CACGTG CAC^GTG BclI 50 TGATCA T^GATCA BfaI 815 CTAG C^TAG BpmI 683 CTGGAG CTGGAG(16/14) BsaHI 1052 GACGCC G[GA]^CG[CT]C BsaI 460 GAGACC GGTCTC(1/5) BsgI 1242 GTGCAG GTGCAG(16/14) Bsp1407I 2 TGTACA T^GTACA BspDI 881 ATCGAT AT^CGAT BspMI 1169 ACCTGC ACCTGC(4/8) BsrBI 1159 GAGCGG CCGCTC(-3/-3) BsrGI 2 TGTACA T^GTACA BstAPI 144 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 497 TTCGAA TT^CGAA BstYI 557 AGATCC [GA]^GATC[CT] ClaI 881 ATCGAT AT^CGAT DraIII 487 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 422 TGGCCA [CT]^GGCC[GA] Eam1105I 1122 GACGATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 719 CTCTTC CTCTTC(1/4) EcoNI 229 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 733 GATATC GAT^ATC EcoT22I 885 ATGCAT ATGCA^T FauI 1039 GCGGG CCCGC(4/6) FbaI 50 TGATCA T^GATCA HgaI 1052 GACGC GACGC(5/10) Hin1I 1052 GACGCC G[GA]^CG[CT]C KpnI 1271 GGTACC GGTAC^C Ksp632I 719 CTCTTC CTCTTC(1/4) MaeI 815 CTAG C^TAG MboII 720 TCTTC GAAGA(8/7) MfeI 203 CAATTG C^AATTG MflI 557 AGATCC [GA]^GATC[CT] MluNI 422 TGGCCA TGG^CCA MscI 422 TGGCCA TGG^CCA MspA1I 61 CAGCTG C[AC]G^C[GT]G MunI 203 CAATTG C^AATTG NlaIV 1271 GGTACC GG[ACGT]^[ACGT]CC NsiI 885 ATGCAT ATGCA^T NspI 301 ACATGC [GA]CATG^[CT] NspV 497 TTCGAA TT^CGAA PaeR7I 1146 CTCGAG C^TCGAG PflFI 270 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 528 CACGTG CAC^GTG PmlI 528 CACGTG CAC^GTG PvuII 61 CAGCTG CAG^CTG SfuI 497 TTCGAA TT^CGAA SmaI 1228 CCCGGG CCC^GGG SnaBI 829 TACGTA TAC^GTA SspBI 2 TGTACA T^GTACA SspI 1192 AATATT AAT^ATT TfiI 495 GATTC G^A[AT]TC Tth111I 270 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1146 CTCGAG C^TCGAG XhoII 557 AGATCC [GA]^GATC[CT] XmaCI 1228 CCCGGG C^CCGGG XmaI 1228 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 1038 CGCG CG^CG 1262 CGCG AcsI 250 GAATTT [GA]^AATT[CT] 948 AAATTC ApoI 250 GAATTT [GA]^AATT[CT] 948 AAATTC AvaI 1146 CTCGAG C^[CT]CG[GA]G 1228 CCCGGG AvaII 27 GGACC G^G[AT]CC 325 GGACC BcnI 1228 CCCGG CC^[GC]GG 1229 CCGGG BsaAI 528 CACGTG [CT]AC^GT[GA] 829 TACGTA BsaWI 1064 ACCGGA [AT]^CCGG[AT] 1274 ACCGGA BsoBI 1146 CTCGAG C^[CT]CG[GA]G 1228 CCCGGG BspHI 449 TCATGA T^CATGA 1235 TCATGA BstUI 1038 CGCG CG^CG 1262 CGCG Cac8I 32 GCCAGC GC[ACGT]^[ACGT]GC 59 GCCAGC CfoI 1037 GCGC GCG^C 1261 GCGC Cfr13I 27 GGACC G^G[ACGT]CC 325 GGACC HaeIII 97 GGCC GG^CC 423 GGCC HhaI 1037 GCGC GCG^C 1261 GCGC HinP1I 1037 GCGC G^CGC 1261 GCGC MvnI 1038 CGCG CG^CG 1262 CGCG NciI 1228 CCCGG CC^[GC]GG 1229 CCGGG PleI 979 GACTC GAGTC(4/5) 1144 GACTC RcaI 449 TCATGA T^CATGA 1235 TCATGA Sau96I 27 GGACC G^G[ACGT]CC 325 GGACC SfcI 345 CTATAG C^T[GA][CT]AG 983 CTGTAG Tsp45I 310 GTCAC ^GT[GC]AC 526 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI Alw44I AlwNI ApaLI AspHI BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HapII HinfI HpaII ItaI MaeII MaeIII MboI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI SmlI TaqI Tru9I TseI Tsp509I TspRI TthHB8I