(Circular) (Six-base) MAPSORT of: pFN21AE4418.seq Check: 2633 from: 1 to: 5998 pFN21AE4418 5998 bp With 182 enzymes: SgfI * March 28, 2012 14:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3341 1937 Size: 1404 4594 Acc65I G'GTAC_C Cuts at: 3276 3319 3276 Size: 43 5955 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2946 3244 4061 5445 5713 1632 Size: 1314 298 817 1384 268 1917 Fragments arranged by size: 1917 1384 1314 817 298 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 3132 4727 3132 Size: 1595 4403 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5998 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2701 2733 2701 Size: 32 5966 AlwNI CAG_nnn'CTG Cuts at: 2155 2225 2566 3741 5313 2155 Size: 70 341 1175 1572 2840 Fragments arranged by size: 2840 1572 1175 341 70 ApaLI G'TGCA_C Cuts at: 2136 3065 3215 4055 5211 2136 Size: 929 150 840 1156 2923 Fragments arranged by size: 2923 1156 929 840 150 ApoI r'AATT_y Cuts at: 1493 2255 2953 3307 3533 3685 5794 1493 Size: 762 698 354 226 152 2109 1697 Fragments arranged by size: 2109 1697 762 698 354 226 152 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 3151 3233 3323 1870 Size: 73 1208 82 90 4545 Fragments arranged by size: 4545 1208 90 82 73 BamHI G'GATC_C Cuts at: 3328 3695 3328 Size: 367 5631 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3276 3319 4774 618 Size: 325 217 627 1489 43 1455 1842 Fragments arranged by size: 1842 1489 1455 627 325 217 43 BanII G_rGCy'C Cuts at: 1391 3317 1391 Size: 1926 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5027 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4189 4223 1903 Size: 34 2252 34 3678 Fragments arranged by size: 3678 2252 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 2055 1548 Size: 507 5491 BfrBI ATG'CAT Cuts at: 2892 2892 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4609 136 Size: 107 122 71 631 605 2937 1525 Fragments arranged by size: 2937 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5993 5993 Size: 5998 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 2140 3069 3192 3219 4059 5215 1790 Size: 350 929 123 27 840 1156 2573 Fragments arranged by size: 2573 1156 929 840 350 123 27 BmgBI CAC'GTC Cuts at: 1318 3246 1318 Size: 1928 4070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2388 2489 3729 Size: 416 53 83 399 24 971 101 1240 Cuts at: 3729 4687 442 Size: 958 1753 Fragments arranged by size: 1753 1240 971 958 416 399 101 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2709 4658 1239 Size: 1470 1949 2579 Fragments arranged by size: 2579 1949 1470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2760 2956 4123 4988 5286 2760 Size: 196 1167 865 298 3472 Fragments arranged by size: 3472 1167 865 298 196 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2459 4661 882 Size: 617 960 2202 2219 Fragments arranged by size: 2219 2202 960 617 BsaAI yAC'GTr Cuts at: 493 1204 2535 2836 3789 493 Size: 711 1331 301 953 2702 Fragments arranged by size: 2702 1331 953 711 301 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3058 3805 4187 4822 Size: 53 83 186 936 1525 747 382 635 Cuts at: 4822 275 Size: 1451 Fragments arranged by size: 1525 1451 936 747 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3069 3279 4431 4829 5103 5250 5661 3069 Size: 210 1152 398 274 147 411 3406 Fragments arranged by size: 3406 1152 411 398 274 210 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5773 Fragments arranged by size: 5773 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5998 BseYI C'CCAG_C Cuts at: 3144 5201 5697 3144 Size: 2057 496 3445 Fragments arranged by size: 3445 2057 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3268 1163 Size: 573 1532 3893 Fragments arranged by size: 3893 1532 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2140 3069 3219 4059 4144 5215 2140 Size: 929 150 840 85 1071 2923 Fragments arranged by size: 2923 1071 929 840 150 85 BsmI GAATG_Cn' Cuts at: 3509 3602 3509 Size: 93 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 3069 3192 3219 3317 4059 4144 Size: 399 350 929 123 27 98 742 85 Cuts at: 4144 5215 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 929 742 399 350 123 98 85 27 BspEI T'CCGG_A Cuts at: 5661 5661 Size: 5998 BspHI T'CATG_A Cuts at: 2454 3240 3887 5617 2454 Size: 786 647 1730 2835 Fragments arranged by size: 2835 1730 786 647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3183 3353 844 Size: 2339 170 3489 Fragments arranged by size: 3489 2339 170 BsrBI CCG'CTC Cuts at: 1394 1823 3166 3885 1394 Size: 429 1343 719 3507 Fragments arranged by size: 3507 1343 719 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4493 4667 65 Size: 1183 3245 174 1396 Fragments arranged by size: 3245 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4642 4829 1700 Size: 110 2832 187 2869 Fragments arranged by size: 2869 2832 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 4087 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 4058 5070 4058 Size: 1012 4986 BstAPI GCAn_nnn'nTGC Cuts at: 794 2155 794 Size: 1361 4637 BstBI TT'CG_AA Cuts at: 2503 4802 2503 Size: 2299 3699 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstYI r'GATC_y Cuts at: 1972 2562 3328 3695 4080 4097 5549 5635 5647 Size: 590 766 367 385 17 1452 86 12 Cuts at: 5647 5993 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 766 590 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5446 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2077 3267 487 Size: 683 56 851 1190 3218 Fragments arranged by size: 3218 1190 851 683 56 BtsI GCAGTG_nn' Cuts at: 3514 4308 4328 3514 Size: 794 20 5184 Fragments arranged by size: 5184 794 20 ClaI AT'CG_AT Cuts at: 1554 2887 1554 Size: 1333 4665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DraIII CAC_nnn'GTG Cuts at: 2497 2497 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2427 3445 4334 Size: 54 1425 50 275 18 597 1018 889 Cuts at: 4334 5963 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1018 889 597 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2730 3928 1353 Size: 1377 1198 3423 Fragments arranged by size: 3423 1377 1198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4585 4959 5105 387 Size: 1064 138 2996 374 146 1280 Fragments arranged by size: 2996 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2709 2946 3244 4061 4658 5445 5713 Size: 393 1077 237 298 817 597 787 268 Cuts at: 5713 1239 Size: 1524 Fragments arranged by size: 1524 1077 817 787 597 393 298 268 237 EcoNI CCTnn'n_nnAGG Cuts at: 2238 2238 Size: 5998 EcoRI G'AATT_C Cuts at: 3307 3685 3307 Size: 378 5620 EcoRV GAT'ATC Cuts at: 2740 2740 Size: 5998 FspI TGC'GCA Cuts at: 4504 4504 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 5145 1678 Size: 3467 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2491 2523 3118 3150 4639 Size: 32 135 32 919 32 595 32 1489 Cuts at: 4639 4671 4713 4745 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1489 919 595 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3342 3588 669 Size: 988 281 1404 246 3079 Fragments arranged by size: 3079 1404 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KpnI G_GTAC'C Cuts at: 3280 3323 3280 Size: 43 5955 MfeI C'AATT_G Cuts at: 2208 3597 2208 Size: 1389 4609 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2686 5112 5296 1305 Size: 1381 2426 184 2007 Fragments arranged by size: 2426 2007 1381 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2429 10 Size: 54 1425 343 597 3579 Fragments arranged by size: 3579 1425 597 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2523 2790 2919 3956 4315 4474 518 Size: 648 1357 267 129 1037 359 159 2042 Fragments arranged by size: 2042 1357 1037 648 359 267 159 129 MspA1I CmG'CkG Cuts at: 1606 2068 3414 4091 5239 5484 1606 Size: 462 1346 677 1148 245 2120 Fragments arranged by size: 2120 1346 1148 677 462 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5446 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NsiI A_TGCA'T Cuts at: 2894 2894 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 2310 3356 4901 1132 Size: 97 1081 1046 1545 2229 Fragments arranged by size: 2229 1545 1081 1046 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5998 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5998 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PmlI CAC'GTG Cuts at: 2535 2535 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4099 5580 5612 4067 Size: 32 1481 32 4453 Fragments arranged by size: 4453 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PspXI vC'TCGA_Gb Cuts at: 3151 3151 Size: 5998 PstI C_TGCA'G Cuts at: 830 3350 830 Size: 2520 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 PvuII CAG'CTG Cuts at: 2068 2068 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SfcI C'TryA_G Cuts at: 826 1045 2350 2988 3346 4481 5162 5353 826 Size: 219 1305 638 358 1135 681 191 1471 Fragments arranged by size: 1471 1305 1135 681 638 358 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 3235 3325 1872 Size: 1363 90 4545 Fragments arranged by size: 4545 1363 90 SmlI C'TyrA_G Cuts at: 820 1017 1943 2775 2971 3151 4102 5003 5265 Size: 197 926 832 196 180 951 901 262 Cuts at: 5265 820 Size: 1553 Fragments arranged by size: 1553 951 926 901 832 262 197 196 180 SnaBI TAC'GTA Cuts at: 493 2836 3789 493 Size: 2343 953 2702 Fragments arranged by size: 2702 2343 953 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3199 3922 5 Size: 47 3147 723 2081 Fragments arranged by size: 3147 2081 723 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5998 StyI C'CwwG_G Cuts at: 513 1065 3437 513 Size: 552 2372 3074 Fragments arranged by size: 3074 2372 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2508 2565 4025 4042 4195 4380 4825 Size: 1255 928 57 1460 17 153 185 445 Cuts at: 4825 325 Size: 1498 Fragments arranged by size: 1498 1460 1255 928 445 185 153 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 4244 5956 Size: 274 80 33 51 1443 30 2237 1712 Cuts at: 5956 96 Size: 138 Fragments arranged by size: 2237 1712 1443 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3048 3080 5958 5990 3048 Size: 32 2878 32 3056 Fragments arranged by size: 3056 2878 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2278 1316 Size: 962 5036 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 3151 1943 Size: 1208 4790 XmaI C'CCGG_G Cuts at: 1870 3233 3323 1870 Size: 1363 90 4545 Fragments arranged by size: 4545 1363 90 XmnI GAAnn'nnTTC Cuts at: 4127 4127 Size: 5998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI