################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HinfI HpaI KpnI MaeII MamI MfeI MroI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcyI 382 GGCGCC G[GA]^CG[CT]C AflIII 161 ACGCGT A^C[GA][CT]GT Asp700I 159 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 589 CCTAGG C^CTAGG BalI 422 TGGCCA TGG^CCA BbeI 382 GGCGCC GGCGC^C BfaI 590 CTAG C^TAG BglII 233 AGATCT A^GATCT BlnI 589 CCTAGG C^CTAGG BlpI 184 GCTCAGC GC^T[ACGT]AGC Bpu1102I 184 GCTCAGC GC^T[ACGT]AGC BsaHI 382 GGCGCC G[GA]^CG[CT]C BsiEI 151 CGGCCG CG[GA][CT]^CG BsrFI 353 ACCGGC [GA]^CCGG[CT] BsrI 549 ACTGG ACTGG(1/-1) BssHII 251 GCGCGC G^CGCGC BstXI 239 CCAAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 233 AGATCT [GA]^GATC[CT] CelII 184 GCTCAGC GC^T[ACGT]AGC Cfr10I 353 ACCGGC [GA]^CCGG[CT] DpnII 234 GATC ^GATC DraII 81 GGGACCC [GA]G^G[ACGT]CC[CT] EagI 151 CGGCCG C^GGCCG EclXI 151 CGGCCG C^GGCCG Eco52I 151 CGGCCG C^GGCCG EcoNI 135 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 81 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 589 CCTAGG C^C[AT][AT]GG HgaI 126 GACGC GACGC(5/10) Hin1I 382 GGCGCC G[GA]^CG[CT]C KasI 382 GGCGCC G^GCGCC KspI 113 CCGCGG CCGC^GG MaeI 590 CTAG C^TAG MaeIII 584 GTGAC ^GT[ACGT]AC MboI 234 GATC ^GATC MflI 233 AGATCT [GA]^GATC[CT] MluI 161 ACGCGT A^CGCGT MluNI 422 TGGCCA TGG^CCA MscI 422 TGGCCA TGG^CCA NarI 382 GGCGCC GG^CGCC NdeII 234 GATC ^GATC PpuMI 81 GGGACCC [GA]G^G[AT]CC[CT] SacII 113 CCGCGG CCGC^GG SapI 533 GCTCTTC GCTCTTC(1/4) Sau3AI 234 GATC ^GATC SfaNI 219 GATGC GCATC(5/9) SfoI 382 GGCGCC GGC^GCC SmaI 121 CCCGGG CCC^GGG StuI 29 AGGCCT AGG^CCT StyI 589 CCTAGG C^C[AT][AT]GG TaqI 505 TCGA T^CGA Tsp45I 584 GTGAC ^GT[GC]AC TthHB8I 505 TCGA T^CGA XhoII 233 AGATCT [GA]^GATC[CT] XmaCI 121 CCCGGG C^CCGGG XmaI 121 CCCGGG C^CCGGG XmnI 159 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AvaI 121 CCCGGG C^[CT]CG[GA]G 378 CTCGGG BanI 382 GGCGCC G^G[CT][GA]CC 673 GGTGCC BsmFI 81 GGGAC GGGAC(10/14) 124 GGGAC BsoBI 121 CCCGGG C^[CT]CG[GA]G 378 CTCGGG DdeI 185 CTCAG C^T[ACGT]AG 578 CTCAG DsaI 56 CCACGG C^C[GA][CT]GG 113 CCGCGG EaeI 151 CGGCCG [CT]^GGCC[GA] 422 TGGCCA EarI 534 CTCTTC CTCTTC(1/4) 410 GAAGAG HphI 144 GGTGA GGTGA(8/7) 562 TCACC Ksp632I 534 CTCTTC CTCTTC(1/4) 410 GAAGAG NlaIII 168 CATG CATG^ 681 CATG SacI 177 GAGCTC GAGCT^C 375 GAGCTC TspRI 256 CAGTG CA[GC]TG[ACGT][ACGT]^ 541 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrBI BssKI BstNI BstUI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV RsaI Sau96I ScrFI TseI