(Circular) (Six-base) MAPSORT of: pFN21AB8900.seq Check: 4310 from: 1 to: 5404 pFN21AB8900 5404 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2340 2340 Size: 5404 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3214 278 Size: 53 83 186 936 1678 2468 Fragments arranged by size: 2468 1678 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2747 1937 Size: 810 4594 Acc65I G'GTAC_C Cuts at: 2725 2725 Size: 5404 AclI AA'CG_TT Cuts at: 1563 3531 3904 1563 Size: 1968 373 3063 Fragments arranged by size: 3063 1968 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3467 4851 5119 1632 Size: 1835 1384 268 1917 Fragments arranged by size: 1917 1835 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5207 AflIII A'CryG_T Cuts at: 1203 2166 4303 5280 1203 Size: 963 2137 977 1327 Fragments arranged by size: 2137 1327 977 963 AgeI A'CCGG_T Cuts at: 4235 4235 Size: 5404 AhdI GACnn_n'nnGTC Cuts at: 4133 4133 Size: 5404 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5404 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5404 AlwNI CAG_nnn'CTG Cuts at: 3147 4719 3147 Size: 1572 3832 ApaLI G'TGCA_C Cuts at: 3461 4617 3461 Size: 1156 4248 ApoI r'AATT_y Cuts at: 1493 2713 2939 3091 5200 1493 Size: 1220 226 152 2109 1697 Fragments arranged by size: 2109 1697 1220 226 152 AseI AT'TA_AT Cuts at: 160 1032 3958 160 Size: 872 2926 1606 Fragments arranged by size: 2926 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5404 AvaI C'yCGr_G Cuts at: 1870 1943 2126 2383 2729 1870 Size: 73 183 257 346 4545 Fragments arranged by size: 4545 346 257 183 73 AvrII C'CTAG_G Cuts at: 2594 2594 Size: 5404 BamHI G'GATC_C Cuts at: 2734 3101 2734 Size: 367 5037 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2387 2678 2725 4180 618 Size: 325 217 627 600 291 47 1455 1842 Fragments arranged by size: 1842 1455 627 600 325 291 217 47 BanII G_rGCy'C Cuts at: 1391 2117 2186 2384 2723 1391 Size: 726 69 198 339 4072 Fragments arranged by size: 4072 726 339 198 69 BbeI G_GCGC'C Cuts at: 2391 2391 Size: 5404 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4433 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3595 3629 1903 Size: 34 1658 34 3678 Fragments arranged by size: 3678 1658 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3296 4506 3296 Size: 1210 4194 BclI T'GATC_A Cuts at: 1548 1548 Size: 5404 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4015 136 Size: 107 122 71 631 605 2343 1525 Fragments arranged by size: 2343 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2238 5399 2238 Size: 3161 2243 BlpI GC'TnA_GC Cuts at: 2190 2821 2190 Size: 631 4773 Bme1580I G_kGCm'C Cuts at: 1790 2683 3465 4621 1790 Size: 893 782 1156 2573 Fragments arranged by size: 2573 1156 893 782 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5404 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3135 4093 442 Size: 416 53 83 399 24 1718 958 1753 Fragments arranged by size: 1753 1718 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5404 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2205 2205 Size: 5404 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2059 2066 2162 2585 4064 1239 Size: 820 7 96 423 1479 2579 Fragments arranged by size: 2579 1479 820 423 96 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3529 4394 4692 3529 Size: 865 298 4241 Fragments arranged by size: 4241 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4067 882 Size: 617 2568 2219 Fragments arranged by size: 2568 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3195 493 Size: 711 1991 2702 Fragments arranged by size: 2702 1991 711 BsaBI GATnn'nnATC Cuts at: 5398 5398 Size: 5404 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2388 3211 3593 4228 Size: 53 83 186 936 855 823 382 635 Cuts at: 4228 275 Size: 1451 Fragments arranged by size: 1451 936 855 823 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3837 4235 4509 4656 5067 3837 Size: 398 274 147 411 4174 Fragments arranged by size: 4174 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5179 Fragments arranged by size: 5179 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2011 2065 2318 1186 Size: 825 54 253 4272 Fragments arranged by size: 4272 825 253 54 BseYI C'CCAG_C Cuts at: 2108 2501 4607 5103 2108 Size: 393 2106 496 2409 Fragments arranged by size: 2409 2106 496 393 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4831 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2159 2854 3615 4643 1540 Size: 275 186 158 695 761 1028 2301 Fragments arranged by size: 2301 1028 761 695 275 186 158 BsiHKAI G_wGCw'C Cuts at: 2186 2384 2576 3465 3550 4621 2186 Size: 198 192 889 85 1071 2969 Fragments arranged by size: 2969 1071 889 198 192 85 BsmI GAATG_Cn' Cuts at: 2915 3008 2915 Size: 93 5311 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2186 2384 2576 2683 2723 3465 Size: 399 327 69 198 192 107 40 742 Cuts at: 3465 3550 4621 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 327 198 192 107 85 69 40 BspEI T'CCGG_A Cuts at: 5067 5067 Size: 5404 BspHI T'CATG_A Cuts at: 3293 5023 3293 Size: 1730 3674 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2340 2529 2643 2759 844 Size: 1496 189 114 116 3489 Fragments arranged by size: 3489 1496 189 116 114 BsrBI CCG'CTC Cuts at: 1394 1823 2098 2189 2443 3291 1394 Size: 429 275 91 254 848 3507 Fragments arranged by size: 3507 848 429 275 254 91 BsrDI GCAATG_nn' Cuts at: 65 1248 3899 4073 65 Size: 1183 2651 174 1396 Fragments arranged by size: 2651 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2358 4048 4235 1700 Size: 110 548 1690 187 2869 Fragments arranged by size: 2869 1690 548 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5404 BssHII G'CGCG_C Cuts at: 1925 2256 1925 Size: 331 5073 BssSI C'ACGA_G Cuts at: 3464 4476 3464 Size: 1012 4392 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5404 BstBI TT'CG_AA Cuts at: 4208 4208 Size: 5404 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5404 BstXI CCAn_nnnn'nTGG Cuts at: 2251 2251 Size: 5404 BstYI r'GATC_y Cuts at: 1972 2238 2734 3101 3486 3503 4955 5041 5053 Size: 266 496 367 385 17 1452 86 12 Cuts at: 5053 5399 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 496 385 367 346 266 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 2118 513 Size: 552 939 57 57 3799 Fragments arranged by size: 3799 939 552 57 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4665 Fragments arranged by size: 4665 683 56 BtsI GCAGTG_nn' Cuts at: 2267 2545 2920 3714 3734 2267 Size: 278 375 794 20 3937 Fragments arranged by size: 3937 794 375 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5404 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5369 DraI TTT'AAA Cuts at: 2708 2708 Size: 5404 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4411 809 Size: 507 3095 1802 Fragments arranged by size: 3095 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2156 2427 2851 Size: 54 1425 50 275 18 326 271 424 Cuts at: 2851 3740 5369 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 424 326 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2156 2851 1537 Size: 275 344 695 4090 Fragments arranged by size: 4090 695 344 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2410 2545 3334 1353 Size: 1057 135 789 3423 Fragments arranged by size: 3423 1057 789 135 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3991 4365 4511 387 Size: 1064 138 2402 374 146 1280 Fragments arranged by size: 2402 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2184 2382 2184 Size: 198 5206 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2066 2162 2585 3467 4064 4851 Size: 393 427 7 96 423 882 597 787 Cuts at: 4851 5119 1239 Size: 268 1524 Fragments arranged by size: 1524 882 787 597 427 423 393 268 96 7 EcoNI CCTnn'n_nnAGG Cuts at: 2144 2144 Size: 5404 EcoO109I rG'GnC_Cy Cuts at: 2087 2087 Size: 5404 EcoRI G'AATT_C Cuts at: 2713 3091 2713 Size: 378 5026 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2311 2311 Size: 5404 FspI TGC'GCA Cuts at: 3910 3910 Size: 5404 HaeII r_GCGC'y Cuts at: 1678 2012 2109 2197 2251 2391 4551 1678 Size: 334 97 88 54 140 2160 2531 Fragments arranged by size: 2531 2160 334 140 97 88 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4045 4077 4119 4151 1373 Size: 32 135 32 2473 32 42 32 2626 Fragments arranged by size: 2626 2473 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2748 2994 669 Size: 988 281 810 246 3079 Fragments arranged by size: 3079 988 810 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5404 HpaI GTT'AAC Cuts at: 2994 2994 Size: 5404 KasI G'GCGC_C Cuts at: 2387 2387 Size: 5404 KpnI G_GTAC'C Cuts at: 2729 2729 Size: 5404 MfeI C'AATT_G Cuts at: 3003 3003 Size: 5404 MluI A'CGCG_T Cuts at: 2166 5280 2166 Size: 3114 2290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4518 4702 1305 Size: 3213 184 2007 Fragments arranged by size: 3213 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2429 10 Size: 54 1425 343 597 2985 Fragments arranged by size: 2985 1425 597 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3362 3721 3880 518 Size: 648 2196 359 159 2042 Fragments arranged by size: 2196 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2120 2403 2433 2820 3497 4645 4890 1606 Size: 514 283 30 387 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 514 387 283 245 30 NarI GG'CG_CC Cuts at: 2388 2388 Size: 5404 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3913 Fragments arranged by size: 3913 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5404 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5404 NotI GC'GGCC_GC Cuts at: 2851 2851 Size: 5404 NspI r_CATG'y Cuts at: 1132 1229 2762 4307 1132 Size: 97 1533 1545 2229 Fragments arranged by size: 2229 1545 1533 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5404 PciI A'CATG_T Cuts at: 4303 4303 Size: 5404 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5404 PmeI GTTT'AAAC Cuts at: 2708 2708 Size: 5404 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3473 3505 4986 5018 3473 Size: 32 1481 32 3859 Fragments arranged by size: 3859 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2087 2087 Size: 5404 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5404 PsiI TTA'TAA Cuts at: 2974 2974 Size: 5404 PstI C_TGCA'G Cuts at: 830 2756 830 Size: 1926 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5404 SacI G_AGCT'C Cuts at: 2186 2384 2186 Size: 198 5206 SacII CC_GC'GG Cuts at: 2121 2121 Size: 5404 SalI G'TCGA_C Cuts at: 1936 2746 1936 Size: 810 4594 SanDI GG'GwC_CC Cuts at: 2087 2087 Size: 5404 SapI GCTCTTCn'nnn_ Cuts at: 2545 2545 Size: 5404 SbfI CC_TGCA'GG Cuts at: 2756 2756 Size: 5404 ScaI AGT'ACT Cuts at: 3652 5364 3652 Size: 1712 3692 SfcI C'TryA_G Cuts at: 826 1045 2752 3887 4568 4759 826 Size: 219 1707 1135 681 191 1471 Fragments arranged by size: 1707 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2389 2389 Size: 5404 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5404 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5404 SmaI CCC'GGG Cuts at: 1872 2128 2731 1872 Size: 256 603 4545 Fragments arranged by size: 4545 603 256 SmlI C'TyrA_G Cuts at: 820 1017 1943 3508 4409 4671 820 Size: 197 926 1565 901 262 1553 Fragments arranged by size: 1565 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3195 493 Size: 2702 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5404 SphI G_CATG'C Cuts at: 1132 2762 1132 Size: 1630 3774 SspI AAT'ATT Cuts at: 5 52 3328 5 Size: 47 3276 2081 Fragments arranged by size: 3276 2081 47 StuI AGG'CCT Cuts at: 1517 2036 1517 Size: 519 4885 StyI C'CwwG_G Cuts at: 513 1065 2004 2594 2843 513 Size: 552 939 590 249 3074 Fragments arranged by size: 3074 939 590 552 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2483 3431 3448 3601 3786 4231 325 Size: 1255 903 948 17 153 185 445 1498 Fragments arranged by size: 1498 1255 948 903 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3650 5362 96 Size: 274 80 33 51 1443 1673 1712 138 Fragments arranged by size: 1712 1673 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5364 5396 5364 Size: 32 5372 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5404 XbaI T'CTAG_A Cuts at: 2740 2740 Size: 5404 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5404 XmaI C'CCGG_G Cuts at: 1870 2126 2729 1870 Size: 256 603 4545 Fragments arranged by size: 4545 603 256 XmnI GAAnn'nnTTC Cuts at: 2168 3533 2168 Size: 1365 4039 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3212 276 Size: 53 83 186 936 1678 2468 Fragments arranged by size: 2468 1678 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SexAI SfiI SrfI SwaI XcmI