################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI StyI SwaI TaqI TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 1195 GACGTC GACGT^C Acc65I 1216 GGTACC G^GTACC AccI 1336 GTCTAC GT^[AC][GT]AC ApaI 410 GGGCCC GGGCC^C Asp700I 1057 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1216 GGTACC G^GTACC AspHI 805 GTGCTC G[AT]GC[AT]^C AspI 1168 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 899 TGGCCA TGG^CCA BamHI 540 GGATCC G^GATCC BanI 1216 GGTACC G^G[CT][GA]CC BanII 410 GGGCCC G[GA]GC[CT]^C BglI 1484 GCCACGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 15 GCTAAGC GC^T[ACGT]AGC BpmI 92 CTCCAG CTGGAG(16/14) Bpu1102I 15 GCTAAGC GC^T[ACGT]AGC BsaAI 1327 TACGTG [CT]AC^GT[GA] BsiEI 59 CGACCG CG[GA][CT]^CG BsiHKAI 805 GTGCTC G[AT]GC[AT]^C BspHI 1403 TCATGA T^CATGA BspMI 1452 GCAGGT ACCTGC(4/8) BsrDI 1072 CATTGC GCAATG(2/0) BssSI 7 CTCGTG CACGAG(-5/-1) BstAPI 773 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 15 GCTAAGC GC^T[ACGT]AGC DrdI 1168 GACAAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1264 CTCTTC CTCTTC(1/4) EcoNI 1401 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 40 AGCGCC [GA]GCGC^[CT] HgaI 824 GCGTC GACGC(5/10) HincII 1198 GTCAAC GT[CT]^[GA]AC HindII 1198 GTCAAC GT[CT]^[GA]AC KpnI 1216 GGTACC GGTAC^C Ksp632I 1264 CTCTTC CTCTTC(1/4) MluNI 899 TGGCCA TGG^CCA MscI 899 TGGCCA TGG^CCA PflFI 1168 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 712 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 137 GGGACCT [GA]G^G[AT]CC[CT] PstI 1290 CTGCAG CTGCA^G RcaI 1403 TCATGA T^CATGA SmaI 123 CCCGGG CCC^GGG SpeI 163 ACTAGT A^CTAGT SspI 418 AATATT AAT^ATT StuI 317 AGGCCT AGG^CCT Tth111I 1168 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 712 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 123 CCCGGG C^CCGGG XmaI 123 CCCGGG C^CCGGG XmnI 1057 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AccII 27 CGCG CG^CG 692 CGCG AciI 26 CCGC CCGC(-3/-1) 234 CCGC AcsI 465 GAATTT [GA]^AATT[CT] 1057 GAATTT AcyI 823 GGCGTC G[GA]^CG[CT]C 1195 GACGTC ApoI 465 GAATTT [GA]^AATT[CT] 1057 GAATTT AvaI 50 CCCGAG C^[CT]CG[GA]G 123 CCCGGG AvaII 138 GGACC G^G[AT]CC 1129 GGTCC BbsI 957 GAAGAC GAAGAC(2/6) 1177 GTCTTC BcgI 1365 CGATTACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 641 GCACTATCATCG BfaI 164 CTAG C^TAG 487 CTAG BmyI 410 GGGCCC G[AGT]GC[ACT]^C 805 GTGCTC BpuAI 957 GAAGAC GAAGAC(2/6) 1177 GTCTTC BsaHI 823 GGCGTC G[GA]^CG[CT]C 1195 GACGTC BseRI 877 CTCCTC GAGGAG(10/8) 571 GAGGAG BsoBI 50 CCCGAG C^[CT]CG[GA]G 123 CCCGGG Bsp1286I 410 GGGCCC G[AGT]GC[ACT]^C 805 GTGCTC BstUI 27 CGCG CG^CG 692 CGCG BstXI 599 CCACTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1132 CCAGTGCAGTGG BstYI 540 GGATCC [GA]^GATC[CT] 923 GGATCT CfoI 28 GCGC GCG^C 41 GCGC DraII 137 GGGACCT [GA]G^G[ACGT]CC[CT] 409 GGGGCCC EaeI 23 TGGCCG [CT]^GGCC[GA] 899 TGGCCA EcoO109I 137 GGGACCT [GA]G^G[ACGT]CC[CT] 409 GGGGCCC HhaI 28 GCGC GCG^C 41 GCGC Hin1I 823 GGCGTC G[GA]^CG[CT]C 1195 GACGTC HinP1I 28 GCGC G^CGC 41 GCGC MaeI 164 CTAG C^TAG 487 CTAG MaeII 1196 ACGT A^CGT 1328 ACGT MflI 540 GGATCC [GA]^GATC[CT] 923 GGATCT MseI 995 TTAA T^TAA 1280 TTAA MslI 456 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 903 CACATGGGTG MspA1I 291 CAGCTG C[AC]G^C[GT]G 745 CAGCTG MvnI 27 CGCG CG^CG 692 CGCG PleI 327 GACTC GAGTC(4/5) 750 GACTC PvuII 291 CAGCTG CAG^CTG 745 CAGCTG SmlI 132 CTTGAG C^T[CT][GA]AG 1256 CTCAAG TfiI 708 GATTC G^A[AT]TC 1311 GATTC Tru9I 995 TTAA T^TAA 1280 TTAA Tsp45I 262 GTGAC ^GT[GC]AC 715 GTGAC XcmI 88 CCATCTCCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 902 CCACATGGGTGCTGG XhoII 540 GGATCC [GA]^GATC[CT] 923 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AfaI AluI AlwI AlwNI BbvI BcnI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp509I TspRI