(Circular) (Six-base) MAPSORT of: pFN21AE1474.seq Check: 491 from: 1 to: 6211 pFN21AE1474 6211 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3448 3448 Size: 6211 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3204 4021 278 Size: 53 83 186 936 1668 817 2468 Fragments arranged by size: 2468 1668 936 817 186 83 53 AccI GT'mk_AC Cuts at: 1937 3342 3554 1937 Size: 1405 212 4594 Fragments arranged by size: 4594 1405 212 Acc65I G'GTAC_C Cuts at: 3221 3532 3221 Size: 311 5900 AclI AA'CG_TT Cuts at: 1563 4338 4711 1563 Size: 2775 373 3063 Fragments arranged by size: 3063 2775 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2688 4274 5658 5926 1632 Size: 1056 1586 1384 268 1917 Fragments arranged by size: 1917 1586 1384 1056 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6014 AflIII A'CryG_T Cuts at: 1203 5110 6087 1203 Size: 3907 977 1327 Fragments arranged by size: 3907 1327 977 AgeI A'CCGG_T Cuts at: 5042 5042 Size: 6211 AhdI GACnn_n'nnGTC Cuts at: 4940 4940 Size: 6211 AleI CACnn'nnGTG Cuts at: 1166 2912 1166 Size: 1746 4465 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3165 1184 Size: 1981 4230 AlwNI CAG_nnn'CTG Cuts at: 2723 2784 3390 3954 5526 2723 Size: 61 606 564 1572 3408 Fragments arranged by size: 3408 1572 606 564 61 ApaI G_GGCC'C Cuts at: 2419 2419 Size: 6211 ApaLI G'TGCA_C Cuts at: 4268 5424 4268 Size: 1156 5055 ApoI r'AATT_y Cuts at: 1493 2470 3062 3520 3746 3898 6007 1493 Size: 977 592 458 226 152 2109 1697 Fragments arranged by size: 2109 1697 977 592 458 226 152 AseI AT'TA_AT Cuts at: 160 1032 4765 160 Size: 872 3733 1606 Fragments arranged by size: 3733 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2128 3536 1870 Size: 73 112 73 1408 4545 Fragments arranged by size: 4545 1408 112 73 73 BamHI G'GATC_C Cuts at: 2545 3541 3908 2545 Size: 996 367 4848 Fragments arranged by size: 4848 996 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3221 3532 4987 618 Size: 325 217 627 1434 311 1455 1842 Fragments arranged by size: 1842 1455 1434 627 325 311 217 BanII G_rGCy'C Cuts at: 1391 2419 3530 1391 Size: 1028 1111 4072 Fragments arranged by size: 4072 1111 1028 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2969 3175 928 Size: 971 1070 206 3964 Fragments arranged by size: 3964 1070 971 206 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2635 2669 3359 3393 4402 4436 1903 Size: 34 698 34 690 34 1009 34 3678 Fragments arranged by size: 3678 1009 698 690 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4103 5313 4103 Size: 1210 5001 BclI T'GATC_A Cuts at: 1548 1548 Size: 6211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3495 4822 136 Size: 107 122 71 631 605 1823 1327 1525 Fragments arranged by size: 1823 1525 1327 631 605 122 107 71 BglII A'GATC_T Cuts at: 6206 6206 Size: 6211 BlpI GC'TnA_GC Cuts at: 2021 3628 2021 Size: 1607 4604 Bme1580I G_kGCm'C Cuts at: 1790 2419 4272 5428 1790 Size: 629 1853 1156 2573 Fragments arranged by size: 2573 1853 1156 629 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2097 2733 3243 Size: 416 53 83 399 24 680 636 510 Cuts at: 3243 3942 4900 442 Size: 699 958 1753 Fragments arranged by size: 1753 958 699 680 636 510 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2082 4871 1239 Size: 843 2789 2579 Fragments arranged by size: 2789 2579 843 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2158 3246 4336 5201 5499 2158 Size: 1088 1090 865 298 2870 Fragments arranged by size: 2870 1090 1088 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4874 882 Size: 617 3375 2219 Fragments arranged by size: 3375 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3334 4002 493 Size: 711 2130 668 2702 Fragments arranged by size: 2702 2130 711 668 BsaBI GATnn'nnATC Cuts at: 6205 6205 Size: 6211 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2829 3201 4018 4400 Size: 53 83 186 936 1296 372 817 382 Cuts at: 4400 5035 275 Size: 635 1451 Fragments arranged by size: 1451 1296 936 817 635 382 372 186 83 53 BsaWI w'CCGG_w Cuts at: 4644 5042 5316 5463 5874 4644 Size: 398 274 147 411 4981 Fragments arranged by size: 4981 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2590 2620 1178 Size: 30 165 30 1187 30 4769 Fragments arranged by size: 4769 1187 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2591 2873 1186 Size: 1405 282 4524 Fragments arranged by size: 4524 1405 282 BseYI C'CCAG_C Cuts at: 2211 5414 5910 2211 Size: 3203 496 2512 Fragments arranged by size: 3203 2512 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2059 2768 3161 3308 3453 1163 Size: 573 323 709 393 147 145 3921 Fragments arranged by size: 3921 709 573 393 323 147 145 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2067 3661 4422 5450 1540 Size: 275 186 66 1594 761 1028 2301 Fragments arranged by size: 2301 1594 1028 761 275 186 66 BsiHKAI G_wGCw'C Cuts at: 2814 4272 4357 5428 2814 Size: 1458 85 1071 3597 Fragments arranged by size: 3597 1458 1071 85 BsmI GAATG_Cn' Cuts at: 3722 3815 3722 Size: 93 6118 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2419 2814 3530 4272 4357 5428 1391 Size: 399 629 395 716 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 716 629 399 395 85 BspEI T'CCGG_A Cuts at: 5874 5874 Size: 6211 BspHI T'CATG_A Cuts at: 3408 4100 5830 3408 Size: 692 1730 3789 Fragments arranged by size: 3789 1730 692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3448 3566 844 Size: 2604 118 3489 Fragments arranged by size: 3489 2604 118 BsrBI CCG'CTC Cuts at: 1394 1823 4098 1394 Size: 429 2275 3507 Fragments arranged by size: 3507 2275 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3076 4706 4880 65 Size: 1183 1828 1630 174 1396 Fragments arranged by size: 1828 1630 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4855 5042 1700 Size: 110 3045 187 2869 Fragments arranged by size: 3045 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6211 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6211 BssSI C'ACGA_G Cuts at: 2012 4271 5283 2012 Size: 2259 1012 2940 Fragments arranged by size: 2940 2259 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2784 794 Size: 1990 4221 BstBI TT'CG_AA Cuts at: 5015 5015 Size: 6211 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6211 BstXI CCAn_nnnn'nTGG Cuts at: 2611 3144 2611 Size: 533 5678 BstYI r'GATC_y Cuts at: 1972 2545 2928 3541 3908 4293 4310 5762 5848 Size: 573 383 613 367 385 17 1452 86 Cuts at: 5848 5860 6206 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 613 573 385 383 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5659 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3231 487 Size: 683 56 2005 3467 Fragments arranged by size: 3467 2005 683 56 BtsI GCAGTG_nn' Cuts at: 2556 3149 3304 3420 3727 4521 4541 2556 Size: 593 155 116 307 794 20 4226 Fragments arranged by size: 4226 794 593 307 155 116 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6211 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6176 DraI TTT'AAA Cuts at: 3515 3515 Size: 6211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3179 5218 809 Size: 507 1863 2039 1802 Fragments arranged by size: 2039 1863 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2904 3658 Size: 54 1425 50 275 18 198 876 754 Cuts at: 3658 4547 6176 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 876 754 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3658 1537 Size: 275 1846 4090 Fragments arranged by size: 4090 1846 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3275 4141 1353 Size: 1922 866 3423 Fragments arranged by size: 3423 1922 866 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4798 5172 5318 387 Size: 1064 138 3209 374 146 1280 Fragments arranged by size: 3209 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 2688 4274 4871 5658 5926 1239 Size: 393 450 606 1586 597 787 268 1524 Fragments arranged by size: 1586 1524 787 606 597 450 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3410 3410 Size: 6211 EcoO109I rG'GnC_Cy Cuts at: 2143 2415 2143 Size: 272 5939 EcoRI G'AATT_C Cuts at: 3520 3898 3520 Size: 378 5833 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2986 2986 Size: 6211 FspI TGC'GCA Cuts at: 4717 4717 Size: 6211 HaeII r_GCGC'y Cuts at: 1678 2049 5358 1678 Size: 371 3309 2531 Fragments arranged by size: 3309 2531 371 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4852 4884 4926 4958 1373 Size: 32 135 32 3280 32 42 32 2626 Fragments arranged by size: 3280 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3205 3555 3801 669 Size: 988 281 1267 350 246 3079 Fragments arranged by size: 3079 1267 988 350 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6211 HpaI GTT'AAC Cuts at: 3801 3801 Size: 6211 KpnI G_GTAC'C Cuts at: 3225 3536 3225 Size: 311 5900 MfeI C'AATT_G Cuts at: 3810 3810 Size: 6211 MluI A'CGCG_T Cuts at: 6087 6087 Size: 6211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2662 2815 5325 5509 1305 Size: 1357 153 2510 184 2007 Fragments arranged by size: 2510 2007 1357 184 153 MscI TGG'CCA Cuts at: 10 64 1489 1832 2906 10 Size: 54 1425 343 1074 3315 Fragments arranged by size: 3315 1425 1074 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2465 2912 4169 4528 4687 518 Size: 648 1299 447 1257 359 159 2042 Fragments arranged by size: 2042 1299 1257 648 447 359 159 MspA1I CmG'CkG Cuts at: 1606 2298 2752 3627 4304 5452 5697 1606 Size: 692 454 875 677 1148 245 2120 Fragments arranged by size: 2120 1148 875 692 677 454 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5659 NdeI CA'TA_TG Cuts at: 387 387 Size: 6211 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6211 NotI GC'GGCC_GC Cuts at: 3658 3658 Size: 6211 NspI r_CATG'y Cuts at: 1132 1229 3569 5114 1132 Size: 97 2340 1545 2229 Fragments arranged by size: 2340 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2611 1348 Size: 1263 4948 PciI A'CATG_T Cuts at: 5110 5110 Size: 6211 PflMI CCAn_nnn'nTGG Cuts at: 1108 2723 1108 Size: 1615 4596 PmeI GTTT'AAAC Cuts at: 3515 3515 Size: 6211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 5793 5825 4280 Size: 32 1481 32 4666 Fragments arranged by size: 4666 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 2143 Size: 6211 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6211 PsiI TTA'TAA Cuts at: 3781 3781 Size: 6211 PspOMI G'GGCC_C Cuts at: 2415 2415 Size: 6211 PstI C_TGCA'G Cuts at: 830 3299 3563 830 Size: 2469 264 3478 Fragments arranged by size: 3478 2469 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6211 PvuII CAG'CTG Cuts at: 2298 2752 2298 Size: 454 5757 SalI G'TCGA_C Cuts at: 1936 3553 1936 Size: 1617 4594 SbfI CC_TGCA'GG Cuts at: 3563 3563 Size: 6211 ScaI AGT'ACT Cuts at: 4459 6171 4459 Size: 1712 4499 SfcI C'TryA_G Cuts at: 826 1045 2293 2385 3295 3559 4694 5375 5566 Size: 219 1248 92 910 264 1135 681 191 Cuts at: 5566 826 Size: 1471 Fragments arranged by size: 1471 1248 1135 910 681 264 219 191 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6211 SmaI CCC'GGG Cuts at: 1872 2130 3538 1872 Size: 258 1408 4545 Fragments arranged by size: 4545 1408 258 SmlI C'TyrA_G Cuts at: 820 1017 1943 2137 3261 4315 5216 5478 820 Size: 197 926 194 1124 1054 901 262 1553 Fragments arranged by size: 1553 1124 1054 926 901 262 197 194 SnaBI TAC'GTA Cuts at: 493 4002 493 Size: 3509 2702 SpeI A'CTAG_T Cuts at: 152 2168 152 Size: 2016 4195 SphI G_CATG'C Cuts at: 1132 3569 1132 Size: 2437 3774 SspI AAT'ATT Cuts at: 5 52 2425 4135 5 Size: 47 2373 1710 2081 Fragments arranged by size: 2373 2081 1710 47 StuI AGG'CCT Cuts at: 1517 2324 1517 Size: 807 5404 StyI C'CwwG_G Cuts at: 513 1065 3507 3650 513 Size: 552 2442 143 3074 Fragments arranged by size: 3074 2442 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2902 4238 4255 4408 4593 5038 325 Size: 1255 1322 1336 17 153 185 445 1498 Fragments arranged by size: 1498 1336 1322 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4457 6169 96 Size: 274 80 33 51 1443 2480 1712 138 Fragments arranged by size: 2480 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2590 2622 2793 2825 6171 6203 2590 Size: 32 171 32 3346 32 2598 Fragments arranged by size: 3346 2598 171 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3176 1316 Size: 1860 4351 XbaI T'CTAG_A Cuts at: 3547 3547 Size: 6211 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2100 2914 2100 Size: 814 5397 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6211 XmaI C'CCGG_G Cuts at: 1870 2128 3536 1870 Size: 258 1408 4545 Fragments arranged by size: 4545 1408 258 XmnI GAAnn'nnTTC Cuts at: 3066 4340 3066 Size: 1274 4937 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3202 4019 276 Size: 53 83 186 936 1668 817 2468 Fragments arranged by size: 2468 1668 936 817 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI