Restriction map for pFN21AE5211
 (Circular) (Six-base) MAPSORT of: pFN21AE5211.seq  Check: 2103  from: 1  to: 5326



pFN21AE5211  5326 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:14  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3136     278 
   Size:         53      83     186     936    1600    2468

  Fragments arranged by size: 

                2468    1600     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2256    2669    1937 
   Size:        319     413    4594

  Fragments arranged by size: 

                4594     413     319
 
Acc65I G'GTAC_C

Cuts at:   2647    2647 
   Size:       5326
 
AclI AA'CG_TT

Cuts at:   1563    3453    3826    1563 
   Size:       1890     373    3063

  Fragments arranged by size: 

                3063    1890     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3389    4773    5041    1632 
   Size:       1757    1384     268    1917

  Fragments arranged by size: 

                1917    1757    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5129
 
AflIII A'CryG_T

Cuts at:   1203    4225    5202    1203 
   Size:       3022     977    1327

  Fragments arranged by size: 

                3022    1327     977
 
AgeI A'CCGG_T

Cuts at:   4157    4157 
   Size:       5326
 
AhdI GACnn_n'nnGTC

Cuts at:   4055    4055 
   Size:       5326
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5326
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5326
 
AlwNI CAG_nnn'CTG

Cuts at:   2073    2608    3069    4641    2073 
   Size:        535     461    1572    2758

  Fragments arranged by size: 

                2758    1572     535     461
 
ApaLI G'TGCA_C

Cuts at:   2593    3383    4539    2593 
   Size:        790    1156    3380

  Fragments arranged by size: 

                3380    1156     790
 
ApoI r'AATT_y

Cuts at:   1493    2635    2861    3013    5122    1493 
   Size:       1142     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1142     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3880     160 
   Size:        872    2848    1606

  Fragments arranged by size: 

                2848    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5326
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2577    2651    1870 
   Size:         73     634      74    4545

  Fragments arranged by size: 

                4545     634      74      73
 
BamHI G'GATC_C

Cuts at:   2656    3023    2656 
   Size:        367    4959
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2647    4102     618 
   Size:        325     217     627     860    1455    1842

  Fragments arranged by size: 

                1842    1455     860     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2022    2645    1391 
   Size:        631     623    4072

  Fragments arranged by size: 

                4072     631     623
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4355
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3517    3551    1903 
   Size:         34    1580      34    3678

  Fragments arranged by size: 

                3678    1580      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2279    3218    4428    2279 
   Size:        939    1210    3177

  Fragments arranged by size: 

                3177    1210     939
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5326
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3937     136 
   Size:        107     122      71     631     605    2265    1525

  Fragments arranged by size: 

                2265    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5321    5321 
   Size:       5326
 
BlpI GC'TnA_GC

Cuts at:   2401    2406    2743    2401 
   Size:          5     337    4984

  Fragments arranged by size: 

                4984     337       5
 
Bme1580I G_kGCm'C

Cuts at:   1790    2597    3387    4543    1790 
   Size:        807     790    1156    2573

  Fragments arranged by size: 

                2573    1156     807     790
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5326
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3057    4015     442 
   Size:        416      53      83     399      24    1640     958    1753

  Fragments arranged by size: 

                1753    1640     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5326
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2421    2517    2421 
   Size:         96    5230
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3986    1239 
   Size:       2747    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2396    2396 
   Size:       5326
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3451    4316    4614    3451 
   Size:        865     298    4163

  Fragments arranged by size: 

                4163     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3989     882 
   Size:        617    2490    2219

  Fragments arranged by size: 

                2490    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3117     493 
   Size:        711    1913    2702

  Fragments arranged by size: 

                2702    1913     711
 
BsaBI GATnn'nnATC

Cuts at:   5320    5320 
   Size:       5326
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3133    3515    4150     275 
   Size:         53      83     186     936    1600     382     635    1451

  Fragments arranged by size: 

                1600    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2247    3759    4157    4431    4578    4989    2247 
   Size:       1512     398     274     147     411    2584

  Fragments arranged by size: 

                2584    1512     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5101

  Fragments arranged by size: 

                5101     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2330    2516    1186 
   Size:       1144     186    3996

  Fragments arranged by size: 

                3996    1144     186
 
BseYI C'CCAG_C

Cuts at:   2063    4529    5025    2063 
   Size:       2466     496    2364

  Fragments arranged by size: 

                2466    2364     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2059    1163 
   Size:        573     323    4430

  Fragments arranged by size: 

                4430     573     323
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2776    3537    4565    1540 
   Size:        275     186     775     761    1028    2301

  Fragments arranged by size: 

                2301    1028     775     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2597    3387    3472    4543    2597 
   Size:        790      85    1071    3380

  Fragments arranged by size: 

                3380    1071     790      85
 
BsmI GAATG_Cn'

Cuts at:   2456    2837    2930    2456 
   Size:        381      93    4852

  Fragments arranged by size: 

                4852     381      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2022    2597    2645    3387    3472    4543    1391 
   Size:        399     232     575      48     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     575     399     232      85      48
            
 
BspEI T'CCGG_A

Cuts at:   4989    4989 
   Size:       5326
 
BspHI T'CATG_A

Cuts at:   3215    4945    3215 
   Size:       1730    3596
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2375    2681     844 
   Size:       1531     306    3489

  Fragments arranged by size: 

                3489    1531     306
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3213    1394 
   Size:        429    1390    3507

  Fragments arranged by size: 

                3507    1390     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2325    2503    3821    3995      65 
   Size:       1183    1077     178    1318     174    1396

  Fragments arranged by size: 

                1396    1318    1183    1077     178     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3970    4157    1700 
   Size:        110    2160     187    2869

  Fragments arranged by size: 

                2869    2160     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5326
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5326
 
BssSI C'ACGA_G

Cuts at:   3386    4398    3386 
   Size:       1012    4314
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2073    2505    2608     794 
   Size:       1279     432     103    3512

  Fragments arranged by size: 

                3512    1279     432     103
 
BstBI TT'CG_AA

Cuts at:   4130    4130 
   Size:       5326
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5326
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2143    2143 
   Size:       5326
 
BstYI r'GATC_y

Cuts at:   1972    2656    3023    3408    3425    4877    4963    4975    5321 
   Size:        684     367     385      17    1452      86      12     346

Cuts at:   5321    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     684     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2227    2227 
   Size:       5326
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2268     513 
   Size:        552     939     264    3571

  Fragments arranged by size: 

                3571     939     552     264
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4587

  Fragments arranged by size: 

                4587     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2842    3636    3656    2842 
   Size:        794      20    4512

  Fragments arranged by size: 

                4512     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5326
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5291
 
DraI TTT'AAA

Cuts at:   2630    2630 
   Size:       5326
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4333     809 
   Size:        507    3017    1802

  Fragments arranged by size: 

                3017    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2773    3662    5291 
   Size:         54    1425      50     275      18     943     889    1629

Cuts at:   5291       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     943     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2773    1537 
   Size:        275     961    4090

  Fragments arranged by size: 

                4090     961     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3256    1353 
   Size:       1903    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3913    4287    4433     387 
   Size:       1064     138    2324     374     146    1280

  Fragments arranged by size: 

                2324    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3389    3986    4773    5041    1239 
   Size:        393    1757     597     787     268    1524

  Fragments arranged by size: 

                1757    1524     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2070    2298    2070 
   Size:        228    5098
 
EcoRI G'AATT_C

Cuts at:   2635    3013    2635 
   Size:        378    4948
 
FspI TGC'GCA

Cuts at:   3832    3832 
   Size:       5326
 
HaeII r_GCGC'y

Cuts at:   1678    2413    4473    1678 
   Size:        735    2060    2531

  Fragments arranged by size: 

                2531    2060     735
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3967    3999    4041    4073    1373 
   Size:         32     135      32    2395      32      42      32    2626

  Fragments arranged by size: 

                2626    2395     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2161    2670    2916     669 
   Size:        988     281     223     509     246    3079

  Fragments arranged by size: 

                3079     988     509     281     246     223
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5326
 
HpaI GTT'AAC

Cuts at:   2916    2916 
   Size:       5326
 
KpnI G_GTAC'C

Cuts at:   2651    2651 
   Size:       5326
 
MfeI C'AATT_G

Cuts at:   2925    2925 
   Size:       5326
 
MluI A'CGCG_T

Cuts at:   5202    5202 
   Size:       5326
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2142    4440    4624    1305 
   Size:        837    2298     184    2007

  Fragments arranged by size: 

                2298    2007     837     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3504

  Fragments arranged by size: 

                3504    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3284    3643    3802     518 
   Size:        648    2118     359     159    2042

  Fragments arranged by size: 

                2118    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2240    2742    3419    4567    4812    1606 
   Size:        634     502     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     634     502     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2268     513 
   Size:        552     939     264    3571

  Fragments arranged by size: 

                3571     939     552     264
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5326
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5326
 
NotI GC'GGCC_GC

Cuts at:   2773    2773 
   Size:       5326
 
NspI r_CATG'y

Cuts at:   1132    1229    2684    4229    1132 
   Size:         97    1455    1545    2229

  Fragments arranged by size: 

                2229    1545    1455      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5326
 
PciI A'CATG_T

Cuts at:   4225    4225 
   Size:       5326
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5326
 
PmeI GTTT'AAAC

Cuts at:   2630    2630 
   Size:       5326
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3395    3427    4908    4940    3395 
   Size:         32    1481      32    3781

  Fragments arranged by size: 

                3781    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2298    2298 
   Size:       5326
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5326
 
PsiI TTA'TAA

Cuts at:   2896    2896 
   Size:       5326
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2201    2233    2201 
   Size:         32    5294
 
PstI C_TGCA'G

Cuts at:    830    2343    2372    2493    2678     830 
   Size:       1513      29     121     185    3478

  Fragments arranged by size: 

                3478    1513     185     121      29
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5326
 
SalI G'TCGA_C

Cuts at:   1936    2668    1936 
   Size:        732    4594
 
SbfI CC_TGCA'GG

Cuts at:   2678    2678 
   Size:       5326
 
ScaI AGT'ACT

Cuts at:   3574    5286    3574 
   Size:       1712    3614
 
SfcI C'TryA_G

Cuts at:    826    1045    2339    2368    2489    2674    3809    4490    4681 
   Size:        219    1294      29     121     185    1135     681     191

Cuts at:   4681     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1294    1135     681     219     191     185     121
                  29
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5326
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5326
 
SmaI CCC'GGG

Cuts at:   1872    2653    1872 
   Size:        781    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3430    4331    4593     820 
   Size:        197     926    1487     901     262    1553

  Fragments arranged by size: 

                1553    1487     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3117     493 
   Size:       2624    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5326
 
SphI G_CATG'C

Cuts at:   1132    2684    1132 
   Size:       1552    3774
 
SspI AAT'ATT

Cuts at:      5      52    3250       5 
   Size:         47    3198    2081

  Fragments arranged by size: 

                3198    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5326
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2268    2530    2765     513 
   Size:        552     939     264     262     235    3074

  Fragments arranged by size: 

                3074     939     552     264     262     235
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2109    3353    3370    3523    3708    4153     325 
   Size:       1255     529    1244      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1244     529     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3572    5284      96 
   Size:        274      80      33      51    1443    1595    1712     138

  Fragments arranged by size: 

                1712    1595    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2446    2478    5286    5318    2446 
   Size:         32    2808      32    2454

  Fragments arranged by size: 

                2808    2454      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2123    1316 
   Size:        807    4519
 
XbaI T'CTAG_A

Cuts at:   2662    2662 
   Size:       5326
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5326
 
XmaI C'CCGG_G

Cuts at:   1870    2651    1870 
   Size:        781    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3455    3455 
   Size:       5326
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3134     276 
   Size:         53      83     186     936    1600    2468

  Fragments arranged by size: 

                2468    1600     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PsrI
     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI    EcoNI
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI    PvuII
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
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