################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BciVI BclI BfrI BglI BglII BlnI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FokI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MfeI MflI MluI MluNI MroI MscI MseI MunI NarI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AluI 561 AGCT AG^CT AlwI 743 GGATC GGATC(4/5) ApaI 24 GGGCCC GGGCC^C Asp700I 330 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 21 CTCGGG C^[CT]CG[GA]G BanII 24 GGGCCC G[GA]GC[CT]^C BbsI 303 GTCTTC GAAGAC(2/6) BbvCI 147 CCTCAGC CCTCAGC(-5/-2) BcgI 13 GCAGCCCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 162 GCTGAGC GC^T[ACGT]AGC BpmI 353 CTGGAG CTGGAG(16/14) Bpu1102I 162 GCTGAGC GC^T[ACGT]AGC BpuAI 303 GTCTTC GAAGAC(2/6) BsaAI 783 CACGTA [CT]AC^GT[GA] BsaBI 601 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 761 CTCCTC GAGGAG(10/8) BsgI 88 GTGCAG GTGCAG(16/14) BsmI 390 GAATGC GAATGC(1/-1) BsoBI 21 CTCGGG C^[CT]CG[GA]G BsrDI 672 CATTGC GCAATG(2/0) BsrFI 182 GCCGGC [GA]^CCGG[CT] BssHII 71 GCGCGC G^CGCGC BstAPI 376 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 162 GCTGAGC GC^T[ACGT]AGC Cfr10I 182 GCCGGC [GA]^CCGG[CT] EcoT22I 731 ATGCAT ATGCA^T HgaI 528 GCGTC GACGC(5/10) HinfI 157 GAGTC G^A[ACGT]TC KspI 57 CCGCGG CCGC^GG MamI 601 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 304 TCTTC GAAGA(8/7) MspA1I 57 CCGCGG C[AC]G^C[GT]G NaeI 182 GCCGGC GCC^GGC NcoI 714 CCATGG C^CATGG NdeI 377 CATATG CA^TATG NgoMIV 182 GCCGGC G^CCGGC NsiI 731 ATGCAT ATGCA^T PleI 157 GAGTC GAGTC(4/5) PstI 563 CTGCAG CTGCA^G SacII 57 CCGCGG CCGC^GG SfcI 563 CTGCAG C^T[GA][CT]AG TspRI 798 CAGTG CA[GC]TG[ACGT][ACGT]^ XbaI 603 TCTAGA T^CTAGA XcmI 797 CCAGTGACGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 330 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 296 GTAC GT^AC 667 GTAC BanI 99 GGTGCC G^G[CT][GA]CC 252 GGTGCC BcnI 111 CCCGG CC^[GC]GG 153 CCGGG BfaI 326 CTAG C^TAG 604 CTAG BmyI 24 GGGCCC G[AGT]GC[ACT]^C 100 GTGCCC Bsp1286I 24 GGGCCC G[AGT]GC[ACT]^C 100 GTGCCC BsrBI 18 CCGCTC CCGCTC(-3/-3) 165 GAGCGG BstNI 171 CCAGG CC^[AT]GG 444 CCTGG DdeI 148 CTCAG C^T[ACGT]AG 163 CTGAG DraII 587 GGGACCC [GA]G^G[ACGT]CC[CT] 753 GGGTCCT EaeI 47 CGGCCA [CT]^GGCC[GA] 168 CGGCCA EcoO109I 587 GGGACCC [GA]G^G[ACGT]CC[CT] 753 GGGTCCT EcoRII 171 CCAGG ^CC[AT]GG 444 CCTGG EcoT14I 83 CCTTGG C^C[AT][AT]GG 714 CCATGG MaeI 326 CTAG C^TAG 604 CTAG MaeII 52 ACGT A^CGT 784 ACGT MslI 246 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 724 CACTATGATG MvaI 171 CCAGG CC^[AT]GG 444 CCTGG NciI 111 CCCGG CC^[GC]GG 153 CCGGG PpuMI 587 GGGACCC [GA]G^G[AT]CC[CT] 753 GGGTCCT RsaI 296 GTAC GT^AC 667 GTAC SfaNI 730 GATGC GCATC(5/9) 733 GCATC StyI 83 CCTTGG C^C[AT][AT]GG 714 CCATGG TaqI 455 TCGA T^CGA 609 TCGA Tsp509I 549 AATT ^AATT 572 AATT TthHB8I 455 TCGA T^CGA 609 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AccII AciI AvaII BbvI BsaJI BsiYI BslI BsmFI BsrI BssKI BstUI Cac8I CfoI Cfr13I DpnII DsaI FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MnlI MspI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I