(Circular) (Six-base) MAPSORT of: pFN21AE2300.seq Check: 4938 from: 1 to: 7276 pFN21AE2300 7276 bp With 182 enzymes: SgfI * April 6, 2011 13:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3442 3442 Size: 7276 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5086 278 Size: 53 83 186 936 3550 2468 Fragments arranged by size: 3550 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4619 1937 Size: 2682 4594 Acc65I G'GTAC_C Cuts at: 4597 4597 Size: 7276 AclI AA'CG_TT Cuts at: 1563 5403 5776 1563 Size: 3840 373 3063 Fragments arranged by size: 3840 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3092 3525 4443 5339 6723 6991 1632 Size: 1460 433 918 896 1384 268 1917 Fragments arranged by size: 1917 1460 1384 918 896 433 268 AfeI AGC'GCT Cuts at: 2295 2295 Size: 7276 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7079 AflIII A'CryG_T Cuts at: 1203 6175 7152 1203 Size: 4972 977 1327 Fragments arranged by size: 4972 1327 977 AgeI A'CCGG_T Cuts at: 6107 6107 Size: 7276 AhdI GACnn_n'nnGTC Cuts at: 6005 6005 Size: 7276 AleI CACnn'nnGTG Cuts at: 1166 4512 1166 Size: 3346 3930 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3568 1184 Size: 2384 4892 AlwNI CAG_nnn'CTG Cuts at: 5019 6591 5019 Size: 1572 5704 ApaLI G'TGCA_C Cuts at: 5333 6489 5333 Size: 1156 6120 ApoI r'AATT_y Cuts at: 1493 2124 2337 2426 2560 2608 2773 2980 3517 Size: 631 213 89 134 48 165 207 537 Cuts at: 3517 3573 4268 4380 4462 4585 4811 4963 7072 Size: 56 695 112 82 123 226 152 2109 Cuts at: 7072 1493 Size: 1697 Fragments arranged by size: 2109 1697 695 631 537 226 213 207 165 152 134 123 112 89 82 56 48 AseI AT'TA_AT Cuts at: 160 1032 4218 5830 160 Size: 872 3186 1612 1606 Fragments arranged by size: 3186 1612 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7276 AvaI C'yCGr_G Cuts at: 1870 1943 2149 4601 1870 Size: 73 206 2452 4545 Fragments arranged by size: 4545 2452 206 73 BamHI G'GATC_C Cuts at: 4606 4973 4606 Size: 367 6909 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4597 6052 618 Size: 325 217 627 2810 1455 1842 Fragments arranged by size: 2810 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4359 4595 1391 Size: 2968 236 4072 Fragments arranged by size: 4072 2968 236 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2100 2870 3086 4092 928 Size: 971 201 770 216 1006 4112 Fragments arranged by size: 4112 1006 971 770 216 201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5467 5501 1903 Size: 34 3530 34 3678 Fragments arranged by size: 3678 3530 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5168 6378 5168 Size: 1210 6066 BclI T'GATC_A Cuts at: 1548 1548 Size: 7276 BfrBI ATG'CAT Cuts at: 3555 3555 Size: 7276 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5887 136 Size: 107 122 71 631 605 4215 1525 Fragments arranged by size: 4215 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4291 7271 4291 Size: 2980 4296 BlpI GC'TnA_GC Cuts at: 4693 4693 Size: 7276 Bme1580I G_kGCm'C Cuts at: 1790 5337 6493 1790 Size: 3547 1156 2573 Fragments arranged by size: 3547 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7276 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2822 5007 5965 Size: 416 53 83 399 24 1405 2185 958 Cuts at: 5965 442 Size: 1753 Fragments arranged by size: 2185 1753 1405 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7276 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3393 4090 5936 1239 Size: 2154 697 1846 2579 Fragments arranged by size: 2579 2154 1846 697 Bpu10I CC'TnA_GC Cuts at: 4353 4353 Size: 7276 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5401 6266 6564 5401 Size: 865 298 6113 Fragments arranged by size: 6113 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5939 882 Size: 617 4440 2219 Fragments arranged by size: 4440 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2390 5067 493 Size: 711 1186 2677 2702 Fragments arranged by size: 2702 2677 1186 711 BsaBI GATnn'nnATC Cuts at: 4254 7270 4254 Size: 3016 4260 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5083 5465 6100 275 Size: 53 83 186 936 3550 382 635 1451 Fragments arranged by size: 3550 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2651 2769 3232 3708 5709 6107 6381 6528 6939 Size: 118 463 476 2001 398 274 147 411 Cuts at: 6939 2651 Size: 2988 Fragments arranged by size: 2988 2001 476 463 411 398 274 147 118 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7051 Fragments arranged by size: 7051 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2609 1186 Size: 1423 5853 BseYI C'CCAG_C Cuts at: 6479 6975 6479 Size: 496 6780 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6703 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4726 5487 6515 1540 Size: 275 186 2725 761 1028 2301 Fragments arranged by size: 2725 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4359 5337 5422 6493 4359 Size: 978 85 1071 5142 Fragments arranged by size: 5142 1071 978 85 BsmI GAATG_Cn' Cuts at: 2924 3557 3984 4787 4880 2924 Size: 633 427 803 93 5320 Fragments arranged by size: 5320 803 633 427 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4359 4595 5337 5422 6493 1391 Size: 399 2569 236 742 85 1071 2174 Fragments arranged by size: 2569 2174 1071 742 399 236 85 BspEI T'CCGG_A Cuts at: 3232 6939 3232 Size: 3707 3569 BspHI T'CATG_A Cuts at: 4258 5165 6895 4258 Size: 907 1730 4639 Fragments arranged by size: 4639 1730 907 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3442 3475 4631 844 Size: 2598 33 1156 3489 Fragments arranged by size: 3489 2598 1156 33 BsrBI CCG'CTC Cuts at: 1394 1823 5163 1394 Size: 429 3340 3507 Fragments arranged by size: 3507 3340 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5771 5945 65 Size: 1183 4523 174 1396 Fragments arranged by size: 4523 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5920 6107 1700 Size: 110 4110 187 2869 Fragments arranged by size: 4110 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7276 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7276 BssSI C'ACGA_G Cuts at: 5336 6348 5336 Size: 1012 6264 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7276 BstBI TT'CG_AA Cuts at: 6080 6080 Size: 7276 BstEII G'GTnAC_C Cuts at: 1184 2765 1184 Size: 1581 5695 BstXI CCAn_nnnn'nTGG Cuts at: 2196 3926 4454 2196 Size: 1730 528 5018 Fragments arranged by size: 5018 1730 528 BstYI r'GATC_y Cuts at: 1972 2942 4291 4606 4973 5358 5375 6827 6913 Size: 970 1349 315 367 385 17 1452 86 Cuts at: 6913 6925 7271 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1349 970 385 367 346 315 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4162 4162 Size: 7276 BtgI C'CryG_G Cuts at: 513 1065 2004 3866 513 Size: 552 939 1862 3923 Fragments arranged by size: 3923 1862 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6537 Fragments arranged by size: 6537 683 56 BtsI GCAGTG_nn' Cuts at: 4792 5586 5606 4792 Size: 794 20 6462 Fragments arranged by size: 6462 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7276 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7241 DraI TTT'AAA Cuts at: 2086 2256 2565 2985 3588 4320 4580 2086 Size: 170 309 420 603 732 260 4782 Fragments arranged by size: 4782 732 603 420 309 260 170 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6283 809 Size: 507 4967 1802 Fragments arranged by size: 4967 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2314 2406 3460 Size: 54 1425 50 275 18 484 92 1054 Cuts at: 3460 4407 4723 5612 7241 8 Size: 947 316 889 1629 43 Fragments arranged by size: 1629 1425 1054 947 889 484 316 275 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4723 1537 Size: 275 2911 4090 Fragments arranged by size: 4090 2911 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2815 5206 1353 Size: 1462 2391 3423 Fragments arranged by size: 3423 2391 1462 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5863 6237 6383 387 Size: 1064 138 4274 374 146 1280 Fragments arranged by size: 4274 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4357 4357 Size: 7276 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3092 3393 3525 4090 4443 5339 5936 Size: 393 1460 301 132 565 353 896 597 Cuts at: 5936 6723 6991 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1460 896 787 597 565 393 353 301 268 132 EcoO109I rG'GnC_Cy Cuts at: 4327 4327 Size: 7276 EcoRI G'AATT_C Cuts at: 2337 2773 4462 4585 4963 2337 Size: 436 1689 123 378 4650 Fragments arranged by size: 4650 1689 436 378 123 EcoRV GAT'ATC Cuts at: 4344 4344 Size: 7276 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3442 3442 Size: 7276 FspI TGC'GCA Cuts at: 5782 5782 Size: 7276 HaeII r_GCGC'y Cuts at: 1678 2297 6423 1678 Size: 619 4126 2531 Fragments arranged by size: 4126 2531 619 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3332 3364 4142 4174 4204 Size: 32 135 32 1760 32 778 32 30 Cuts at: 4204 4236 5917 5949 5991 6023 1373 Size: 32 1681 32 42 32 2626 Fragments arranged by size: 2626 1760 1681 778 135 42 32 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 669 1657 1938 2161 2833 3450 4399 4620 4866 Size: 988 281 223 672 617 949 221 246 Cuts at: 4866 669 Size: 3079 Fragments arranged by size: 3079 988 949 672 617 281 246 223 221 HindIII A'AGCT_T Cuts at: 748 2113 748 Size: 1365 5911 HpaI GTT'AAC Cuts at: 2161 2833 4399 4866 2161 Size: 672 1566 467 4571 Fragments arranged by size: 4571 1566 672 467 KpnI G_GTAC'C Cuts at: 4601 4601 Size: 7276 MfeI C'AATT_G Cuts at: 2040 2286 4875 2040 Size: 246 2589 4441 Fragments arranged by size: 4441 2589 246 MluI A'CGCG_T Cuts at: 7152 7152 Size: 7276 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3787 6390 6574 1305 Size: 2482 2603 184 2007 Fragments arranged by size: 2603 2482 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2316 2408 3462 4409 10 Size: 54 1425 343 484 92 1054 947 2877 Fragments arranged by size: 2877 1425 1054 947 484 343 92 54 MslI CAynn'nnrTG Cuts at: 518 1166 3331 3800 3806 3924 4371 4512 5234 Size: 648 2165 469 6 118 447 141 722 Cuts at: 5234 5593 5752 518 Size: 359 159 2042 Fragments arranged by size: 2165 2042 722 648 469 447 359 159 141 118 6 MspA1I CmG'CkG Cuts at: 1606 2584 4692 5369 6517 6762 1606 Size: 978 2108 677 1148 245 2120 Fragments arranged by size: 2120 2108 1148 978 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3866 513 Size: 552 939 1862 3923 Fragments arranged by size: 3923 1862 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7276 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7276 NotI GC'GGCC_GC Cuts at: 4723 4723 Size: 7276 NsiI A_TGCA'T Cuts at: 3557 3557 Size: 7276 NspI r_CATG'y Cuts at: 1132 1229 2743 4241 4634 6179 1132 Size: 97 1514 1498 393 1545 2229 Fragments arranged by size: 2229 1545 1514 1498 393 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7276 PciI A'CATG_T Cuts at: 6175 6175 Size: 7276 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7276 PmeI GTTT'AAAC Cuts at: 4580 4580 Size: 7276 PmlI CAC'GTG Cuts at: 2390 2390 Size: 7276 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5345 5377 6858 6890 5345 Size: 32 1481 32 5731 Fragments arranged by size: 5731 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4327 4327 Size: 7276 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7276 PsiI TTA'TAA Cuts at: 4846 4846 Size: 7276 PstI C_TGCA'G Cuts at: 830 4628 830 Size: 3798 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7276 PvuII CAG'CTG Cuts at: 2584 2584 Size: 7276 SacI G_AGCT'C Cuts at: 4359 4359 Size: 7276 SalI G'TCGA_C Cuts at: 1936 4618 1936 Size: 2682 4594 SanDI GG'GwC_CC Cuts at: 4327 4327 Size: 7276 SbfI CC_TGCA'GG Cuts at: 4628 4628 Size: 7276 ScaI AGT'ACT Cuts at: 5524 7236 5524 Size: 1712 5564 SfcI C'TryA_G Cuts at: 826 1045 2139 2511 4510 4624 5759 6440 6631 Size: 219 1094 372 1999 114 1135 681 191 Cuts at: 6631 826 Size: 1471 Fragments arranged by size: 1999 1471 1135 1094 681 372 219 191 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7276 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7276 SmaI CCC'GGG Cuts at: 1872 4603 1872 Size: 2731 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5380 6281 6543 820 Size: 197 926 3437 901 262 1553 Fragments arranged by size: 3437 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5067 493 Size: 4574 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7276 SphI G_CATG'C Cuts at: 1132 4634 1132 Size: 3502 3774 SspI AAT'ATT Cuts at: 5 52 3823 5200 5 Size: 47 3771 1377 2081 Fragments arranged by size: 3771 2081 1377 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7276 StyI C'CwwG_G Cuts at: 513 1065 2004 3378 3866 4410 4715 513 Size: 552 939 1374 488 544 305 3074 Fragments arranged by size: 3074 1374 939 552 544 488 305 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5303 5320 5473 5658 6103 325 Size: 1255 3723 17 153 185 445 1498 Fragments arranged by size: 3723 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3792 4176 5522 Size: 274 80 33 51 1443 1815 384 1346 Cuts at: 5522 7234 96 Size: 1712 138 Fragments arranged by size: 1815 1712 1443 1346 384 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7236 7268 7236 Size: 32 7244 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7276 XbaI T'CTAG_A Cuts at: 2909 4612 2909 Size: 1703 5573 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7276 XmaI C'CCGG_G Cuts at: 1870 4601 1870 Size: 2731 4545 XmnI GAAnn'nnTTC Cuts at: 2295 2962 3577 5405 2295 Size: 667 615 1828 4166 Fragments arranged by size: 4166 1828 667 615 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5084 276 Size: 53 83 186 936 3550 2468 Fragments arranged by size: 3550 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI