Restriction map for pFN21AE2473
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        AocI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvrII       BbrPI       BbvCI       BclI        
BfrI        BglII       BlnI        BsaBI       BseAI       
BsiWI       BsmI        BspDI       BspEI       BssHII      
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        Eam1105I    Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MluI        MroI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI          708  GTCTAC               GT^[AC][GT]AC
Alw44I       1783  GTGCAC               G^TGCAC
ApaLI        1783  GTGCAC               G^TGCAC
BamHI        1001  GGATCC               G^GATCC
BcgI         2327  GCACCACCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2271  GGATAC               GTATCC(6/5)
BfaI          115  CTAG                 C^TAG
BsaAI         217  TACGTG               [CT]AC^GT[GA]
BsaWI        1985  TCCGGT               [AT]^CCGG[AT]
BsiEI        2058  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1619  TGTACA               T^GTACA
BspHI         654  TCATGA               T^CATGA
BsrGI        1619  TGTACA               T^GTACA
BstAPI         14  GCAACTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1968  GGTGACC              G^GT[ACGT]ACC
BstPI        1968  GGTGACC              G^GT[ACGT]ACC
DraIII       1293  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2058  CGGCCG               C^GGCCG
EclXI        2058  CGGCCG               C^GGCCG
Eco52I       2058  CGGCCG               C^GGCCG
EcoO65I      1968  GGTGACC              G^GT[ACGT]ACC
MaeI          115  CTAG                 C^TAG
MfeI         1632  CAATTG               C^AATTG
MseI         2027  TTAA                 T^TAA
MunI         1632  CAATTG               C^AATTG
NcoI         2516  CCATGG               C^CATGG
PaeR7I        764  CTCGAG               C^TCGAG
PpuMI        1724  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI          654  TCATGA               T^CATGA
SacI         1063  GAGCTC               GAGCT^C
SfiI          268  GGCCTGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI        1619  TGTACA               T^GTACA
StuI         1976  AGGCCT               AGG^CCT
TfiI          178  GATTC                G^A[AT]TC
Tru9I        2027  TTAA                 T^TAA
XhoI          764  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AlwNI         400  CAGCGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1501  CAGAACCTG           
Aor51HI      1471  AGCGCT               AGC^GCT
             2265  AGCGCT              
AspI          623  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2014  GACACTGTC           
AviII         986  TGCGCA               TGC^GCA
             1546  TGCGCA              
BalI          236  TGGCCA               TGG^CCA
             2306  TGGCCA              
BbeI          352  GGCGCC               GGCGC^C
             1088  GGCGCC              
BbsI         2497  GAAGAC               GAAGAC(2/6)
              928  GTCTTC              
BlpI         1149  GCTCAGC              GC^T[ACGT]AGC
             2178  GCTGAGC             
Bpu1102I     1149  GCTCAGC              GC^T[ACGT]AGC
             2178  GCTGAGC             
BpuAI        2497  GAAGAC               GAAGAC(2/6)
              928  GTCTTC              
BsgI         1057  CTGCAC               GTGCAG(16/14)
             1009  GTGCAG              
BsmBI        1022  CGTCTC               CGTCTC(1/5)
             1381  GAGACG              
BspLU11I     1688  ACATGT               A^CATGT
             2459  ACATGT              
BstYI        1001  GGATCC               [GA]^GATC[CT]
             2378  AGATCC              
CelII        1149  GCTCAGC              GC^T[ACGT]AGC
             2178  GCTGAGC             
DsaI           38  CCGTGG               C^C[GA][CT]GG
             2516  CCATGG              
Eco47III     1471  AGCGCT               AGC^GCT
             2265  AGCGCT              
EcoNI        1646  CCTACTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1732  CCTGGAGCAGG         
FseI          195  GGCCGGCC             GGCCGG^CC
              264  GGCCGGCC            
FspI          986  TGCGCA               TGC^GCA
             1546  TGCGCA              
KasI          352  GGCGCC               G^GCGCC
             1088  GGCGCC              
MflI         1001  GGATCC               [GA]^GATC[CT]
             2378  AGATCC              
MluNI         236  TGGCCA               TGG^CCA
             2306  TGGCCA              
MscI          236  TGGCCA               TGG^CCA
             2306  TGGCCA              
NarI          352  GGCGCC               GG^CGCC
             1088  GGCGCC              
PflFI         623  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2014  GACACTGTC           
PflMI         666  CCAAGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1500  CCAGAACCTGG         
SapI         1884  GAAGAGC              GCTCTTC(1/4)
              139  GCTCTTC             
SexAI        1157  ACCTGGT              A^CC[AT]GGT
             1505  ACCTGGT             
SfoI          352  GGCGCC               GGC^GCC
             1088  GGCGCC              
SmaI          293  CCCGGG               CCC^GGG
             1346  CCCGGG              
Tth111I       623  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2014  GACACTGTC           
Van91I        666  CCAAGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1500  CCAGAACCTGG         
XcmI          511  CCACAGTGGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2330  CCACCATCGTAGTGG     
XhoII        1001  GGATCC               [GA]^GATC[CT]
             2378  AGATCC              
XmaCI         293  CCCGGG               C^CCGGG
             1346  CCCGGG              
XmaI          293  CCCGGG               C^CCGGG
             1346  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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