################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I CelII CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MunI NarI NheI NotI NruI NsiI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 625 CGCG CG^CG AcyI 374 GACGCC G[GA]^CG[CT]C Alw44I 1652 GTGCAC G^TGCAC Aor51HI 206 AGCGCT AGC^GCT ApaLI 1652 GTGCAC G^TGCAC AspI 858 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 560 CCCGGG C^[CT]CG[GA]G BbsI 336 GAAGAC GAAGAC(2/6) BbvCI 988 GCTGAGG CCTCAGC(-5/-2) BfaI 835 CTAG C^TAG BpuAI 336 GAAGAC GAAGAC(2/6) BsaBI 126 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 374 GACGCC G[GA]^CG[CT]C BsiEI 621 CGATCG CG[GA][CT]^CG BsoBI 560 CCCGGG C^[CT]CG[GA]G BspDI 619 ATCGAT AT^CGAT BssSI 1677 CACGAG CACGAG(-5/-1) BstBI 1076 TTCGAA TT^CGAA BstUI 625 CGCG CG^CG CfoI 207 GCGC GCG^C ClaI 619 ATCGAT AT^CGAT DraIII 707 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1627 CCATGG C^C[GA][CT]GG Eco47III 206 AGCGCT AGC^GCT EcoRI 361 GAATTC G^AATTC HaeII 206 AGCGCT [GA]GCGC^[CT] HgaI 374 GACGC GACGC(5/10) HhaI 207 GCGC GCG^C Hin1I 374 GACGCC G[GA]^CG[CT]C HinP1I 207 GCGC G^CGC MaeI 835 CTAG C^TAG MamI 126 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 625 CGCG CG^CG NaeI 984 GCCGGC GCC^GGC NcoI 1627 CCATGG C^CATGG NdeI 356 CATATG CA^TATG NgoMIV 984 GCCGGC G^CCGGC NspV 1076 TTCGAA TT^CGAA PflFI 858 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 879 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1175 CTGCAG CTGCA^G PvuI 621 CGATCG CGAT^CG PvuII 227 CAGCTG CAG^CTG SfuI 1076 TTCGAA TT^CGAA SmaI 560 CCCGGG CCC^GGG SmlI 1687 CTCAAG C^T[CT][GA]AG StuI 479 AGGCCT AGG^CCT TspRI 711 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 858 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 879 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 860 CCAAGTCTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 560 CCCGGG C^CCGGG XmaI 560 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 198 GTAC GT^AC 762 GTAC AlwNI 1025 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1291 CAGTTACTG AocI 754 CCTGAGG CC^T[ACGT]AGG 1009 CCTGAGG AspHI 1034 GTGCTC G[AT]GC[AT]^C 1652 GTGCAC BamHI 290 GGATCC G^GATCC 1325 GGATCC BglII 831 AGATCT A^GATCT 1694 AGATCT BsaI 856 GAGACC GGTCTC(1/5) 1346 GAGACC BsiHKAI 1034 GTGCTC G[AT]GC[AT]^C 1652 GTGCAC BsrBI 1381 GAGCGG CCGCTC(-3/-3) 1453 GAGCGG BsrDI 527 GCAATG GCAATG(2/0) 1465 GCAATG BstXI 279 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 516 CCAGACCTATGG Bsu36I 754 CCTGAGG CC^T[ACGT]AGG 1009 CCTGAGG Eco81I 754 CCTGAGG CC^T[ACGT]AGG 1009 CCTGAGG MspA1I 227 CAGCTG C[AC]G^C[GT]G 1747 CAGCGG PpuMI 998 AGGACCC [GA]G^G[AT]CC[CT] 1814 GGGACCT RsaI 198 GTAC GT^AC 762 GTAC Tsp45I 587 GTGAC ^GT[GC]AC 737 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TthHB8I XhoII