Restriction map for pFN21AB0404
 (Linear) (Six-base) MAPSORT of: KIBB0404.seq  Check: 3574  from: 1  to: 1836



KIBB0404.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     279     880    1836 
   Size:        279     601     956

  Fragments arranged by size: 

                 956     601     279
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355    1459    1655    1664    1745    1836 
   Size:        355    1104     196       9      81      91

  Fragments arranged by size: 

                1104     355     196      91      81       9
 
AfeI AGC'GCT

Cuts at:      0     208    1836 
   Size:        208    1628
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1030    1296    1836 
   Size:       1030     266     540

  Fragments arranged by size: 

                1030     540     266
 
ApaLI G'TGCA_C

Cuts at:      0    1652    1836 
   Size:       1652     184
 
ApoI r'AATT_y

Cuts at:      0     361    1303    1825    1836 
   Size:        361     942     522      11

  Fragments arranged by size: 

                 942     522     361      11
 
AvaI C'yCGr_G

Cuts at:      0     560    1836 
   Size:        560    1276
 
BamHI G'GATC_C

Cuts at:      0     290    1325    1836 
   Size:        290    1035     511

  Fragments arranged by size: 

                1035     511     290
 
BanI G'GyrC_C

Cuts at:      0     873     981    1713    1836 
   Size:        873     108     732     123

  Fragments arranged by size: 

                 873     732     123     108
 
BanII G_rGCy'C

Cuts at:      0     310     638     901    1792    1836 
   Size:        310     328     263     891      44

  Fragments arranged by size: 

                 891     328     310     263      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     343    1836 
   Size:        343    1493
 
BbvCI CC'TCA_GC

Cuts at:      0     989    1836 
   Size:        989     847
 
BglII A'GATC_T

Cuts at:      0     831    1694    1836 
   Size:        831     863     142

  Fragments arranged by size: 

                 863     831     142
 
Bme1580I G_kGCm'C

Cuts at:      0    1656    1810    1836 
   Size:       1656     154      26

  Fragments arranged by size: 

                1656     154      26
 
BmgBI CAC'GTC

Cuts at:      0     177    1836 
   Size:        177    1659
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     468     704    1306    1836 
   Size:        468     236     602     530

  Fragments arranged by size: 

                 602     530     468     236
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     338    1369    1836 
   Size:        338    1031     467

  Fragments arranged by size: 

                1031     467     338
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     251    1531    1778    1783    1836 
   Size:        118     133    1280     247       5      53

  Fragments arranged by size: 

                1280     247     133     118      53       5
 
Bpu10I CC'TnA_GC

Cuts at:      0     989    1836 
   Size:        989     847
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1672    1836 
   Size:       1672     164
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     850    1340    1836 
   Size:        850     490     496

  Fragments arranged by size: 

                 850     496     490
 
BsaBI GATnn'nnATC

Cuts at:      0     130    1836 
   Size:        130    1706
 
BsaHI Gr'CG_yC

Cuts at:      0     375    1836 
   Size:        375    1461
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     115     180     274     662     685     772     835     991 
   Size:        115      65      94     388      23      87      63     156

Cuts at:    991    1006    1123    1261    1836 
   Size:         15     117     138     575

  Fragments arranged by size: 

                 575     388     156     138     117     115      94      87
                  65      63      23      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     617     701    1081    1836 
   Size:        617      84     380     755

  Fragments arranged by size: 

                 755     617     380      84
 
BsiEI CG_ry'CG

Cuts at:      0     624    1836 
   Size:        624    1212
 
BsiHKAI G_wGCw'C

Cuts at:      0    1038    1656    1836 
   Size:       1038     618     180

  Fragments arranged by size: 

                1038     618     180
 
Bsp1286I G_dGCh'C

Cuts at:      0     310     638     901    1038    1656    1792    1810    1836 
   Size:        310     328     263     137     618     136      18      26

  Fragments arranged by size: 

                 618     328     310     263     137     136      26      18
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     279     589     880    1053    1836 
   Size:        279     310     291     173     783

  Fragments arranged by size: 

                 783     310     291     279     173
 
BsrBI CCG'CTC

Cuts at:      0    1383    1455    1836 
   Size:       1383      72     381

  Fragments arranged by size: 

                1383     381      72
 
BsrDI GCAATG_nn'

Cuts at:      0     534    1472    1836 
   Size:        534     938     364

  Fragments arranged by size: 

                 938     534     364
 
BsrFI r'CCGG_y

Cuts at:      0      73     984    1349    1836 
   Size:         73     911     365     487

  Fragments arranged by size: 

                 911     487     365      73
 
BssSI C'ACGA_G

Cuts at:      0    1677    1836 
   Size:       1677     159
 
BstBI TT'CG_AA

Cuts at:      0    1077    1836 
   Size:       1077     759
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     286     523    1836 
   Size:        286     237    1313

  Fragments arranged by size: 

                1313     286     237
 
BstYI r'GATC_y

Cuts at:      0     131     290     831    1325    1694    1836 
   Size:        131     159     541     494     369     142

  Fragments arranged by size: 

                 541     494     369     159     142     131
 
Bsu36I CC'TnA_GG

Cuts at:      0     755    1010    1836 
   Size:        755     255     826

  Fragments arranged by size: 

                 826     755     255
 
BtgI C'CryG_G

Cuts at:      0    1627    1836 
   Size:       1627     209
 
ClaI AT'CG_AT

Cuts at:      0     620    1836 
   Size:        620    1216
 
DraIII CAC_nnn'GTG

Cuts at:      0     712    1836 
   Size:        712    1124
 
EarI CTCTTCn'nnn_

Cuts at:      0    1238    1640    1721    1836 
   Size:       1238     402      81     115

  Fragments arranged by size: 

                1238     402     115      81
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      22    1612    1836 
   Size:         22    1590     224

  Fragments arranged by size: 

                1590     224      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     251     355    1459    1531    1655    1664    1745 
   Size:        118     133     104    1104      72     124       9      81

Cuts at:   1745    1778    1783    1836 
   Size:         33       5      53

  Fragments arranged by size: 

                1104     133     124     118     104      81      72      53
                  33       9       5
 
EcoO109I rG'GnC_Cy

Cuts at:      0     283     557     838     963     999    1815    1836 
   Size:        283     274     281     125      36     816      21

  Fragments arranged by size: 

                 816     283     281     274     125      36      21
 
EcoRI G'AATT_C

Cuts at:      0     361    1836 
   Size:        361    1475
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1713    1836 
   Size:       1713     123
 
HaeII r_GCGC'y

Cuts at:      0     210    1836 
   Size:        210    1626
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     365     397     625     657     797     829     847     879 
   Size:        365      32     228      32     140      32      18      32

Cuts at:    879    1836 
   Size:        957

  Fragments arranged by size: 

                 957     365     228     140      32      32      32      32
                  18
 
MspA1I CmG'CkG

Cuts at:      0     229    1749    1836 
   Size:        229    1520      87

  Fragments arranged by size: 

                1520     229      87
 
NaeI GCC'GGC

Cuts at:      0     986    1836 
   Size:        986     850
 
NcoI C'CATG_G

Cuts at:      0    1627    1836 
   Size:       1627     209
 
NdeI CA'TA_TG

Cuts at:      0     357    1836 
   Size:        357    1479
 
NgoMIV G'CCGG_C

Cuts at:      0     984    1836 
   Size:        984     852
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     885    1836 
   Size:        885     951
 
PfoI T'CCnGG_A

Cuts at:      0    1403    1836 
   Size:       1403     433
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     562     594    1836 
   Size:        562      32    1242

  Fragments arranged by size: 

                1242     562      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     999    1815    1836 
   Size:        999     816      21

  Fragments arranged by size: 

                 999     816      21
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1277    1309    1836 
   Size:       1277      32     527

  Fragments arranged by size: 

                1277     527      32
 
PstI C_TGCA'G

Cuts at:      0    1179    1836 
   Size:       1179     657
 
PvuI CG_AT'CG

Cuts at:      0     624    1836 
   Size:        624    1212
 
PvuII CAG'CTG

Cuts at:      0     229    1836 
   Size:        229    1607
 
SfcI C'TryA_G

Cuts at:      0     748    1175    1595    1836 
   Size:        748     427     420     241

  Fragments arranged by size: 

                 748     427     420     241
 
SmaI CCC'GGG

Cuts at:      0     562    1836 
   Size:        562    1274
 
SmlI C'TyrA_G

Cuts at:      0    1687    1836 
   Size:       1687     149
 
StuI AGG'CCT

Cuts at:      0     481    1836 
   Size:        481    1355
 
StyI C'CwwG_G

Cuts at:      0     279     476     905    1627    1836 
   Size:        279     197     429     722     209

  Fragments arranged by size: 

                 722     429     279     209     197
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     723    1358    1836 
   Size:        723     635     478

  Fragments arranged by size: 

                 723     635     478
 
TatI w'GTAC_w

Cuts at:      0     197     761    1836 
   Size:        197     564    1075

  Fragments arranged by size: 

                1075     564     197
 
Tth111I GACn'n_nGTC

Cuts at:      0     861    1836 
   Size:        861     975
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     867    1836 
   Size:        867     969
 
XmaI C'CCGG_G

Cuts at:      0     560    1836 
   Size:        560    1276
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI    ApaLI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI    BglII Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BseRI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI    BstBI    BstXI    BstYI   Bsu36I     BtgI     ClaI   DraIII
     EarI     EciI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   MspA1I     NaeI     NcoI     NdeI   NgoMIV    PflMI     PfoI     PpiI
    PpuMI     PsrI     PstI     PvuI    PvuII     SfcI     SmaI     SmlI
     StuI     StyI    TaqII     TatI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
     BmtI    BsaAI    BsaWI    BsaXI    BseYI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrGI   BssHII   BstAPI   BstEII  BstZ17I    BtgZI
     BtsI    CspCI     DraI     DrdI     EaeI     EagI  EcoICRI    EcoNI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NarI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI     XbaI     XhoI     XmnI     ZraI
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