################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsgI BsiHKAI BsiWI BsmBI BsmFI Bsp1407I BspHI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MseI MunI MvnI NarI NcoI NdeI NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 122 TCCGGA T^CCGGA AflIII 353 ACATGT A^C[GA][CT]GT AlwI 120 GATCC GGATC(4/5) Asp700I 387 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 176 CCCGGG C^[CT]CG[GA]G AvaII 168 GGTCC G^G[AT]CC BanII 179 GGGCTC G[GA]GC[CT]^C BbvCI 475 GCTGAGG CCTCAGC(-5/-2) BbvI 312 GCTGC GCAGC(8/12) BclI 146 TGATCA T^GATCA BfaI 35 CTAG C^TAG BglI 209 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 179 GGGCTC G[AGT]GC[ACT]^C BpmI 529 CTCCAG CTGGAG(16/14) BsaWI 122 TCCGGA [AT]^CCGG[AT] BseAI 122 TCCGGA T^CCGGA BsiEI 345 CGATCG CG[GA][CT]^CG BsmI 263 GAATGC GAATGC(1/-1) BsoBI 176 CCCGGG C^[CT]CG[GA]G Bsp1286I 179 GGGCTC G[AGT]GC[ACT]^C BspDI 343 ATCGAT AT^CGAT BspEI 122 TCCGGA T^CCGGA BspLU11I 353 ACATGT A^CATGT BspMI 485 GCAGGT ACCTGC(4/8) BsrFI 308 GCCGGC [GA]^CCGG[CT] BstYI 119 AGATCC [GA]^GATC[CT] Cfr10I 308 GCCGGC [GA]^CCGG[CT] ClaI 343 ATCGAT AT^CGAT FbaI 146 TGATCA T^GATCA Fnu4HI 312 GCTGC GC^[ACGT]GC HphI 378 GGTGA GGTGA(8/7) ItaI 312 GCTGC GC^[ACGT]GC MaeI 35 CTAG C^TAG MflI 119 AGATCC [GA]^GATC[CT] MroI 122 TCCGGA T^CCGGA MslI 279 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 45 CCGCTG C[AC]G^C[GT]G NaeI 308 GCCGGC GCC^GGC NgoMIV 308 GCCGGC G^CCGGC NspI 353 ACATGT [GA]CATG^[CT] PvuI 345 CGATCG CGAT^CG SapI 520 GAAGAGC GCTCTTC(1/4) SfaNI 185 GATGC GCATC(5/9) SmaI 176 CCCGGG CCC^GGG TseI 312 GCTGC G^C[AT]GC XhoII 119 AGATCC [GA]^GATC[CT] XmaCI 176 CCCGGG C^CCGGG XmaI 176 CCCGGG C^CCGGG XmnI 387 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 248 GTAC GT^AC 369 GTAC BcnI 176 CCCGG CC^[GC]GG 177 CCGGG BsaI 82 GAGACC GGTCTC(1/5) 577 GAGACC BseRI 288 GAGGAG GAGGAG(10/8) 544 GAGGAG BsmAI 82 GAGAC GTCTC(1/5) 577 GAGAC HaeII 512 AGCGCC [GA]GCGC^[CT] 524 AGCGCC HinfI 331 GAATC G^A[ACGT]TC 408 GATTC MaeIII 163 GTGAC ^GT[ACGT]AC 277 GTCAC NciI 176 CCCGG CC^[GC]GG 177 CCGGG RsaI 248 GTAC GT^AC 369 GTAC TaqI 183 TCGA T^CGA 344 TCGA TfiI 331 GAATC G^A[AT]TC 408 GATTC Tsp45I 163 GTGAC ^GT[GC]AC 277 GTCAC TthHB8I 183 TCGA T^CGA 344 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I FauI FokI HaeIII HapII HhaI HinP1I HpaII Ksp632I MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI StyI